breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
RA | 278,744 | +A | intergenic (‑239/+75) | PP_0224 ← / ← PP_0225 | Monooxygenase, DszC family/sulfur compound ABC transporter ‑ ATP‑binding subunit |
RA | 307,866 | +C | intergenic (+881/‑988) | PP_0252 → / → PP_0254 | 33 kDa chaperonin/conserved protein of unknown function |
RA | 336,125 | +T | coding (210/237 nt) | PP_0278 → | conserved protein of unknown function |
RA | 353,067 | A→G | intergenic (+34/+84) | PP_0293 → / ← PP_0294 | Imidazole glycerol phosphate synthase subunit HisF/choline / betaine / carnitine ABC transporter ‑ ATP binding subunit |
RA | 499,203 | A→G | intergenic (+47/‑249) | PP_0410 → / → PP_0411 | DNA‑binding response regulator UhpA/Polyamine ABC transporter, ATP‑binding protein |
RA | 1,070,248 | +GA | intergenic (+48/+94) | PP_0927 → / ← PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger related‑protein |
RA | 1,126,647 | +C | intergenic (‑101/+48) | PP_0987 ← / ← PP_0988 | L‑serine dehydratase/glycine dehydrogenase |
RA | 1,419,549 | +C | intergenic (‑38/+98) | PP_1242 ← / ← PP_1244 | conserved protein of unknown function/conserved protein of unknown function |
RA | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | PP_1311 → / → PP_1312 | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
RA | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | PP_1311 → / → PP_1312 | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
RA | 1,499,508 | +C | intergenic (+85/‑79) | PP_1311 → / → PP_1312 | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
RA | 1,938,272 | +C | intergenic (‑107/+43) | PP_1731 ← / ← PP_1732 | 23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor |
RA | 2,964,155 | C→T | A428V (GCA→GTA) | PP_2589 → | Aldehyde dehydrogenase family protein |
RA | 3,074,368 | G→A | S592N (AGC→AAC) | PP_2679 → | quinoprotein ethanol dehydrogenase |
RA | 3,284,241 | +T | intergenic (‑103/‑105) | xylE (Ppu opt) ← / → xylA (Ppu opt) | –/– |
RA | 3,286,869 | G→T | G395W (GGG→TGG) | xylB (Ppu opt) → | |
RA | 3,857,849 | +G | intergenic (‑72/+26) | PP_3394 ← / ← PP_3395 | putative 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/Transcriptional regulator, LysR family |
RA | 3,963,303 | T→C | intergenic (‑123/+72) | PP_3486 ← / ← PP_3487 | putative cytochrome c/conserved exported protein of unknown function |
RA | 3,963,305 | A→T | intergenic (‑125/+70) | PP_3486 ← / ← PP_3487 | putative cytochrome c/conserved exported protein of unknown function |
RA | 4,598,174 | 2 bp→TC | intergenic (+113/+101) | PP_4061 → / ← PP_4063 | conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase |
RA | 4,598,179 | +G | intergenic (+118/+97) | PP_4061 → / ← PP_4063 | conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase |
RA | 4,598,201 | +C | intergenic (+140/+75) | PP_4061 → / ← PP_4063 | conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase |
RA | 4,752,955 | (T)7→6 | intergenic (+48/+705) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,752,960 | T→G | intergenic (+53/+700) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,752,963 | 2 bp→CT | intergenic (+56/+696) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,373 | A→C | intergenic (+466/+287) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,375 | C→G | intergenic (+468/+285) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,378 | C→T | intergenic (+471/+282) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,380 | 2 bp→TG | intergenic (+473/+279) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,383 | A→G | intergenic (+476/+277) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,387 | 3 bp→TGC | intergenic (+480/+271) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,399 | C→T | intergenic (+492/+261) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,401 | A→C | intergenic (+494/+259) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,404 | 2 bp→GC | intergenic (+497/+255) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,407 | A→C | intergenic (+500/+253) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,416 | A→G | intergenic (+509/+244) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,418 | A→C | intergenic (+511/+242) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,420 | G→C | intergenic (+513/+240) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,422 | C→T | intergenic (+515/+238) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,427 | +T | intergenic (+520/+233) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,433 | C→G | intergenic (+526/+227) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,436 | C→T | intergenic (+529/+224) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,438 | C→G | intergenic (+531/+222) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,440 | A→C | intergenic (+533/+220) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
RA | 4,753,442 | C→T | intergenic (+535/+218) | PP_4194 → / ← PP_4195 | citrate synthase/putative oxidoreductase |
JC | 4,992,729 | +GGC | intergenic (‑24/+44) | PP_4387 ← / ← PP_4388 | conserved exported protein of unknown function/Flagellar hook protein FlgE |
RA | 5,566,684 | +GCC | intergenic (‑84/+87) | PP_4887 ← / ← PP_4888 | conserved protein of unknown function/putative methyl‑accepting chemotaxis transducer |
RA | 5,685,827 | +G | intergenic (+113/+27) | PP_4980 → / ← PP_4981 | ATP‑dependent RNA helicase/polyisoprene‑binding protein, acidic stress response factor |
RA | 5,685,829 | G→C | intergenic (+115/+25) | PP_4980 → / ← PP_4981 | ATP‑dependent RNA helicase/polyisoprene‑binding protein, acidic stress response factor |
JC | 5,692,491 | +CGGG | intergenic (‑133/+71) | PP_4986 ← / ← PP_4987 | Channel protein, hemolysin III family/putative Chemotaxis protein |
RA | 6,024,961 | T→C | intergenic (+90/+20) | PP_5265 → / ← PP_5266 | Xanthine phosphoribosyltransferase/putative acetyl‑CoA hydrolase family protein |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | JE3692‑4 | 171113–172965 | 176363–172967 | 3–5251 | 52 [50] | [50] 51 | PP_16SA–PP_5SA | PP_16SA,PP_23SA,PP_5SA |
* | * | ÷ | JE3692‑4 | 176570–178395 | 181778–178397 | 3–5209 | 52 [50] | [50] 52 | PP_16SB–[PP_r01] | PP_16SB,PP_23SB,[PP_r01] |
* | * | ÷ | JE3692‑4 | 1652986 | 1652986 | 1 | 66 [0] | [0] 77 | nahF (Ppu opt)/PP_1445 | –/carbohydrate‑selective porin |
* | * | ÷ | JE3692‑4 | 4820304 | 4820363 | 60 | 64 [0] | [50] 51 | PP_4234/PP_4235 | Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | JE3692‑4 | = 652782 | 91 (0.930) | 50 (0.640) | 30/158 | 2.7 | 58.0% | coding (1961/1962 nt) | PP_0562 | Methyl‑accepting chemotaxis protein CtpL |
? | JE3692‑4 | = 4820303 | 0 (0.000) | intergenic (‑268/‑287) | PP_4234/PP_4235 | Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2 | |||||
* | ? | JE3692‑4 | = 1644999 | 0 (0.000) | 66 (0.680) | 53/196 | 1.0 | 100% | intergenic (‑258/‑188) | PP_1443/araE (Ppu opt) | DNA‑binding, ATP‑dependent protease/– |
? | JE3692‑4 | = 1652985 | 0 (0.000) | intergenic (+174/+34) | nahF (Ppu opt)/PP_1445 | –/carbohydrate‑selective porin | |||||
* | ? | JE3692‑4 | 1644999 = | 0 (0.000) | 74 (0.760) | 57/196 | 0.7 | 100% | intergenic (‑258/‑188) | PP_1443/araE (Ppu opt) | DNA‑binding, ATP‑dependent protease/– |
? | JE3692‑4 | 1652987 = | 0 (0.000) | intergenic (+176/+32) | nahF (Ppu opt)/PP_1445 | –/carbohydrate‑selective porin | |||||
* | ? | JE3692‑4 | 4727628 = | 48 (0.490) | 97 (1.060) | 61/186 | 0.4 | 66.9% | coding (715/1932 nt) | PP_4173 | Sensor histidine kinase/response regulator |
? | JE3692‑4 | = 4727669 | 51 (0.550) | coding (756/1932 nt) | PP_4173 | Sensor histidine kinase/response regulator |