breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 310 (T)9→8 intergenic (+55/‑27) thrL → / → thrA thr operon leader peptide/bifunctional aspartate kinase/homoserine dehydrogenase I
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,903 T→G D27E (GAT→GAG folA → type 3 dihydrofolate reductase
RA 49,905 T→G L28R (CTC→CGC)  folA → type 3 dihydrofolate reductase
RA 60,340 T→C M3V (ATG→GTG)  rluA ← bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
RA 130,120 T→C D673D (GAT→GAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA 168,561 A→G T205A (ACG→GCG)  fhuB → Fe(3+)‑hydroxamate ABC transporter permease FhuB
RA 196,898 T→A intergenic (+51/‑71) bamA → / → skp outer membrane protein assembly factor BamA/molecular chaperone Skp
RA 204,632 T→C V1007A (GTC→GCC)  dnaE → DNA polymerase III subunit alpha
RA 209,618 A→C A267A (GCA→GCC tilS → tRNA lysidine(34) synthetase TilS
RA 232,843 T→C I97T (ATC→ACC)  dnaQ → DNA polymerase III subunit epsilon
RA 238,914 A→C F293V (TTT→GTT)  fadE ← acyl‑CoA dehydrogenase FadE
RA 243,919 A→T R241W (AGG→TGG)  yafL → C40 family peptidase
RA 250,930 A→G I425T (ATC→ACC)  pepD ← cytosol nonspecific dipeptidase
RA 251,113 A→G I364T (ATC→ACC)  pepD ← cytosol nonspecific dipeptidase
RA 253,379 T→C G122G (GGT→GGC frsA → esterase FrsA
RA 277,390 T→C D102G (GAC→GGC)  yagA ← IS481‑like element ISEc19 family transposase
RA 281,176 A→T Q24L (CAG→CTG)  yagG → MFS transporter
RA 295,216 G→C A477G (GCC→GGC)  paoC ← aldehyde oxidoreductase molybdenum‑binding subunit PaoC
RA 386,451 T→C pseudogene (745/1527 nt) BW25113_RS01915 → outer membrane autotransporter barrel domain‑containing protein
RA 441,393 A→C F244V (TTC→GTC)  yajR ← MFS transporter
RA 453,695 G→A A272T (GCG→ACG)  clpX → ATP‑dependent protease ATP‑binding subunit ClpX
RA 453,912 A→G E344G (GAA→GGA)  clpX → ATP‑dependent protease ATP‑binding subunit ClpX
RA 469,392 T→C L324P (CTC→CCC)  amtB → ammonium transporter AmtB
RA 475,405 Δ1 bp intergenic (‑31/+141) maa ← / ← hha maltose O‑acetyltransferase/HHA domain‑containing protein
RA 481,730 Δ1 bp coding (514/648 nt) acrR → multidrug efflux transporter transcriptional repressor AcrR
RA 485,143 A→G D1051G (GAT→GGT)  mscK → mechanosensitive channel MscK
RA 487,218 A→C K117N (AAA→AAC apt → adenine phosphoribosyltransferase
RA 506,540 T→C D99G (GAC→GGC)  copA ← copper‑exporting P‑type ATPase CopA
RA 509,698 A→C T84P (ACG→CCG)  cueR → Cu(I)‑responsive transcriptional regulator
RA 524,350 T→C F85F (TTT→TTC BW25113_RS24960 → hypothetical protein
RA 527,133 A→G V182A (GTT→GCT)  allS ← HTH‑type transcriptional activator AllS
RA 552,665 T→C E178G (GAA→GGA)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
RA 572,447 Δ1 bp intergenic (‑166/‑407) BW25113_RS02855 ← / → essD porin/phage lysis protein EssD
RA 587,628 A→G F336S (TTC→TCC)  nrfB ← cyclic di‑3',5'‑guanylate‑activated glycosyltransferase NrfB
RA 592,602 C→T Q6* (CAA→TAA)  cusF → Cu(+)/Ag(+) efflux RND transporter periplasmic metallochaperone CusF
RA 634,477 A→G E26G (GAA→GGA)  ahpC → alkyl hydroperoxide reductase subunit C
RA 635,449 T→C S81P (TCC→CCC)  ahpF → alkyl hydroperoxide reductase subunit F
RA 668,149 A→G N697N (AAT→AAC leuS ← leucine‑‑tRNA ligase
RA 675,373 A→G E137G (GAA→GGA)  ybeU → DUF1266 domain‑containing protein
RA 678,913 A→G intergenic (‑64/+20) hscC ← / ← rihA molecular chaperone HscC/pyrimidine‑specific ribonucleoside hydrolase RihA
RA 715,111 A→G I269T (ATC→ACC)  speF ← ornithine decarboxylase SpeF
RA 716,126 A→G intergenic (‑210/+48) speF ← / ← BW25113_RS26090 ornithine decarboxylase SpeF/leader peptide SpeFL
RA 725,137 C→A G33G (GGC→GGA rhsC → rhs element protein RhsC
RA 728,467 T→C I1143I (ATT→ATC rhsC → rhs element protein RhsC
RA 736,034 A→T I358F (ATC→TTC)  phrB → deoxyribodipyrimidine photo‑lyase
RA 737,899 A→G N38N (AAT→AAC dtpD ← dipeptide permease DtpD
RA 747,264 T→C D124G (GAT→GGT)  ybgQ ← fimbrial biogenesis outer membrane usher protein
RA 753,287 G→A S48N (AGC→AAC)  sdhB → succinate dehydrogenase iron‑sulfur subunit SdhB
RA 757,924 T→C L316P (CTG→CCG)  odhB → 2‑oxoglutarate dehydrogenase complex dihydrolipoyllysine‑residue succinyltransferase
RA 771,555 A→G E84G (GAG→GGG)  tolR → colicin uptake protein TolR
RA 771,797 A→G intergenic (+64/‑1) tolR → / → tolA colicin uptake protein TolR/cell envelope integrity protein TolA
RA 781,531 T→C I148T (ATC→ACC)  aroG → 3‑deoxy‑7‑phosphoheptulonate synthase AroG
RA 791,624 T→C S103P (TCG→CCG)  modB → molybdate ABC transporter permease subunit
RA 803,404 A→C intergenic (‑39/+20) ybhB ← / ← bioA kinase inhibitor/adenosylmethionine‑‑8‑amino‑7‑oxononanoate transaminase
RA 832,557 T→C S251P (TCC→CCC)  hcxB → hydroxycarboxylate dehydrogenase HcXB
RA 834,220 A→G D148D (GAT→GAC ybiX ← PKHD‑type hydroxylase YbiX
RA 857,539 T→C E175G (GAA→GGA)  ybiW ← glycyl radical protein
RA 858,772 T→C I66V (ATT→GTT)  ybiY ← glycyl‑radical enzyme activating protein
RA 861,316 T→C I169V (ATT→GTT)  moeA ← molybdopterin molybdotransferase MoeA
RA 870,468 A→G Q678Q (CAA→CAG pdeI → EAL domain‑containing protein
RA 871,743 A→G E318G (GAA→GGA)  dgcI → GGDEF domain‑containing protein
RA 885,786 T→G L81R (CTG→CGG)  ybjM → inner membrane protein YbjM
RA 900,176 T→G V43G (GTT→GGT)  ybjQ → heavy metal‑binding domain‑containing protein
RA 923,801 A→G V284A (GTC→GCC)  cydC ← cysteine/glutathione ABC transporter ATP‑binding protein/permease CydC
RA 928,200 T→C R50R (CGT→CGC lrp → leucine‑responsive transcriptional regulator Lrp
RA 945,384 (T)5→4 coding (261/591 nt) ycaK → NAD(P)H oxidoreductase
RA 960,653 T→C M293T (ATG→ACG)  ycaI → ComEC family protein
RA 965,365 T→C M174T (ATG→ACG)  ycaQ → winged helix DNA‑binding protein YcaQ
RA 967,323 Δ1 bp coding (116/894 nt) ycbJ → YcbJ family phosphotransferase
RA 974,173 T→C S798P (TCC→CCC)  mukB → chromosome partition protein MukB
RA 977,344 A→G D281G (GAC→GGC)  ldtD → L,D‑transpeptidase
RA 1,011,464 T→C I155V (ATC→GTC)  fabA ← bifunctional 3‑hydroxydecanoyl‑ACP dehydratase/trans‑2‑decenoyl‑ACP isomerase
RA 1,026,705 A→C V57G (GTG→GGG)  yccA ← FtsH protease modulator YccA
RA 1,056,058 T→C I449I (ATT→ATC torA → trimethylamine‑N‑oxide reductase TorA
RA 1,068,442 A→C G323G (GGT→GGG rutA ← pyrimidine utilization protein A
RA 1,079,189 T→C S120P (TCC→CCC)  efeB → iron uptake transporter deferrochelatase/peroxidase subunit
RA 1,079,811 A→C D327A (GAC→GCC)  efeB → iron uptake transporter deferrochelatase/peroxidase subunit
RA 1,097,764 A→G N78N (AAT→AAC csgE ← curli production assembly/transport protein CsgE
RA 1,124,635 T→C R447R (CGT→CGC murJ → murein biosynthesis integral membrane protein MurJ
RA 1,136,234 A→G E249G (GAA→GGA)  flgL → flagellar hook‑associated protein FlgL
RA 1,149,506 T→C S251P (TCC→CCC)  pabC → aminodeoxychorismate lyase
RA 1,156,804 A→C G68G (GGT→GGG fhuE ← ferric‑rhodotorulic acid/ferric‑coprogen receptor FhuE
RA 1,161,212 T→C intergenic (+71/‑329) ycfP → / → ndh alpha/beta hydrolase YcfP/NADH‑quinone dehydrogenase
RA 1,182,954 T→A D248V (GAT→GTT)  roxA ← [50S ribosomal protein L16]‑arginine 3‑hydroxylase
RA 1,187,888 T→C E67G (GAA→GGA)  hflD ← high frequency lysogenization protein HflD
RA 1,200,135 (A)5→4 pseudogene (279/1137 nt) BW25113_RS05965 → terminase large subunit
RA 1,201,626 A→G pseudogene (34/786 nt) BW25113_RS05980 → baseplate J/gp47 family protein
RA 1,220,495 T→A D108V (GAT→GTT)  minD ← septum site‑determining protein MinD
RA 1,223,250 T→C V27V (GTT→GTC BW25113_RS06140 → YcgL domain‑containing protein
RA 1,235,908 T→G A540A (GCA→GCC cvrA ← potassium/proton antiporter
RA 1,246,085 (C)5→4 coding (210/1071 nt) dhaK ← dihydroxyacetone kinase subunit DhaK
RA 1,251,036 T→G T125P (ACA→CCA)  ycgV ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA 1,260,284 A→G D372G (GAC→GGC)  hemA → glutamyl‑tRNA reductase
RA 1,274,887 A→G intergenic (+83/‑433) narK → / → narG nitrate transporter NarK/nitrate reductase subunit alpha
RA 1,283,900 A→G S61P (TCC→CCC)  purU ← formyltetrahydrofolate deformylase
RA 1,306,087 A→G intergenic (+22/+18) tonB → / ← yciA TonB system transport protein TonB/acyl‑CoA thioester hydrolase YciA
RA 1,336,067 G→A intergenic (+83/‑111) lapB → / → pyrF lipopolysaccharide assembly protein LapB/orotidine‑5'‑phosphate decarboxylase
RA 1,422,040 A→C V315G (GTG→GGG)  BW25113_RS07180 ← IS5‑like element ISKpn26 family transposase
RA 1,449,980 A→G D187G (GAT→GGT)  paaC → phenylacetate‑CoA oxygenase subunit PaaC
RA 1,455,278 T→G R43R (CGT→CGG paaJ → bifunctional 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
RA 1,461,422 (G)5→4 pseudogene (1774/2526 nt) BW25113_RS07350 → autotransporter domain‑containing protein
RA 1,492,661 A→G M517V (ATG→GTG)  opgD → glucan biosynthesis protein OpgD
RA 1,503,720 T→C intergenic (+1/‑45) hicA → / → hicB type II toxin‑antitoxin system mRNA interferase toxin HicA/type II toxin‑antitoxin system antitoxin HicB
RA 1,506,028 G→A A40T (GCC→ACC)  ydcS → ABC transporter substrate‑binding protein
RA 1,507,050 T→G G380G (GGT→GGG ydcS → ABC transporter substrate‑binding protein
RA 1,542,720 A→G T355A (ACT→GCT)  fdnG → formate dehydrogenase‑N subunit alpha
RA 1,575,730 T→C D351G (GAT→GGT)  ydeN ← sulfatase
RA 1,578,188 A→G I10T (ATC→ACC)  safA ← two‑component system connector SafA
RA 1,578,845 A→G D633D (GAT→GAC ydeP ← acid resistance putative oxidoreductase YdeP
RA 1,580,214 T→C D177G (GAC→GGC)  ydeP ← acid resistance putative oxidoreductase YdeP
RA 1,594,535 T→C intergenic (‑69/+10) lsrK ← / ← lsrR autoinducer‑2 kinase/transcriptional regulator LsrR
RA 1,599,483 A→C D59A (GAT→GCT)  lsrB → autoinducer 2 ABC transporter substrate‑binding protein LsrB
RA 1,608,701 C→A R87L (CGC→CTC)  sad ← succinate‑semialdehyde dehydrogenase
RA 1,609,438 T→C P126P (CCT→CCC yneJ → LysR family transcriptional regulator
RA 1,609,528 A→C I156I (ATA→ATC yneJ → LysR family transcriptional regulator
RA 1,613,590 A→C T72P (ACC→CCC)  marR → multiple antibiotic resistance transcriptional regulator MarR
RA 1,613,994 G→A G55D (GGC→GAC)  marA → MDR efflux pump AcrAB transcriptional activator MarA
RA 1,622,039 A→G E89G (GAG→GGG)  ydfG → bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG
RA 1,638,057 A→C W169G (TGG→GGG)  ydfU ← DUF968 domain‑containing protein
RA 1,643,588 T→G intergenic (+290/‑278) BW25113_RS08235 → / → dicB hypothetical protein/cell division inhibition protein DicB
RA 1,645,059 A→G pseudogene (725/768 nt) BW25113_RS23850 → exonuclease
RA 1,659,452 (T)7→6 intergenic (+75/‑84) dmsD → / → clcB Tat proofreading chaperone DmsD/voltage‑gated ClC‑type chloride channel ClcB
RA 1,701,793 A→C P206P (CCA→CCC rsxC → electron transport complex subunit RsxC
RA 1,707,599 A→T I192F (ATC→TTC)  dtpA → dipeptide/tripeptide permease DtpA
RA 1,708,538 A→G intergenic (+10/‑96) dtpA → / → gstA dipeptide/tripeptide permease DtpA/glutathione transferase GstA
RA 1,712,188 T→C D26G (GAT→GGT)  pdxH ← pyridoxamine 5'‑phosphate oxidase
RA 1,712,684 T→C intergenic (‑32/+66) mliC ← / ← anmK C‑type lysozyme inhibitor/anhydro‑N‑acetylmuramic acid kinase
RA 1,718,241 Δ1 bp coding (1864/2013 nt) ydhK → FUSC family protein
RA 1,727,442 T→G F1367V (TTT→GTT)  lhr → ATP‑dependent helicase
RA 1,732,351 (A)7→6 coding (251/1026 nt) purR → HTH‑type transcriptional repressor PurR
RA 1,737,390 T→C E37G (GAA→GGA)  ribE ← riboflavin synthase
RA 1,745,340 +G coding (633/639 nt) ydhW ← YdhW family putative oxidoreductase system protein
RA 1,765,154 A→G N95D (AAT→GAT)  ydiL → YdiL family protein
RA 1,769,436 A→G D165G (GAC→GGC)  aroD → type I 3‑dehydroquinate dehydratase
RA 1,776,037 T→G V129G (GTT→GGT)  ydiS → FAD‑dependent oxidoreductase
RA 1,776,541 T→G V297G (GTG→GGG)  ydiS → FAD‑dependent oxidoreductase
RA 1,801,453 T→G V276G (GTT→GGT)  pfkB → 6‑phosphofructokinase II
RA 1,809,395 T→G G424G (GGT→GGG katE → catalase HPII
RA 1,810,989 A→G F135S (TTC→TCC)  chbG ← chitin disaccharide deacetylase
RA 1,834,793 A→G D357G (GAT→GGT)  ynjE → thiosulfate sulfurtransferase YnjE
RA 1,856,434 A→G S85P (TCA→CCA)  msrB ← peptide‑methionine (R)‑S‑oxide reductase MsrB
RA 1,879,784 T→G V288G (GTG→GGG)  yeaW → carnitine monooxygenase subunit YeaW
RA 1,891,886 Δ1 bp coding (698/1365 nt) sdaA → L‑serine ammonia‑lyase
RA 1,900,350 A→G T136A (ACC→GCC)  mntP → manganese efflux pump MntP
RA 1,932,990 A→G E362G (GAA→GGA)  pyk → pyruvate kinase
RA 1,941,091 A→G E195G (GAA→GGA)  yebB → YebB family permuted papain‑like enzyme
RA 1,955,016 A→G D233G (GAC→GGC)  argS → arginine‑‑tRNA ligase
RA 2,000,880 A→G D415G (GAC→GGC)  amyA → alpha‑amylase
RA 2,030,709 A→G S309P (TCG→CCG)  hprS ← two‑component system sensor histidine kinase HprS
RA 2,037,050 T→G intergenic (‑12/‑82) BW25113_RS10320 ← / → mtfA tRNA‑Ser/DgsA anti‑repressor MtfA
RA 2,039,724 A→G T436A (ACA→GCA)  yeeJ → inverse autotransporter adhesin YeeJ
RA 2,066,165 A→G G382G (GGA→GGG agn43 → autotransporter adhesin Ag43
RA 2,069,946 A→G Y53C (TAT→TGT)  yeeS → RadC family protein
RA 2,088,811 T→C V69A (GTC→GCC)  hisA → 1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
RA 2,115,706 A→G F252S (TTC→TCC)  cpsG ← colanic acid biosynthesis phosphomannomutase CpsG
RA 2,135,155 T→C N181S (AAC→AGC)  dcd ← dCTP deaminase
RA 2,136,211 A→G D73D (GAT→GAC udk ← uridine kinase
RA 2,179,484 A→G E30G (GAA→GGA)  rcnA → nickel/cobalt efflux protein RcnA
RA 2,184,600 A→C G160G (GGT→GGG yehC ← fimbria/pilus periplasmic chaperone
RA 2,201,820 A→G K125K (AAA→AAG yehP → VWA domain‑containing protein
RA 2,206,195 A→G F83L (TTT→CTT)  btsR ← two‑component system response regulator BtsR
RA 2,240,933 A→G F360S (TTC→TCC)  lysP ← lysine‑specific permease
RA 2,258,402 T→A I354N (ATC→AAC)  setB → sugar efflux transporter SetB
RA 2,268,854 A→G S35G (AGT→GGT)  yejE → ABC transporter permease
RA 2,287,796 C→T L196L (CTG→CTA ccmF ← cytochrome c‑type biogenesis heme lyase CcmF
RA 2,290,049 A→C V152G (GTG→GGG)  ccmB ← heme exporter protein CcmB
RA 2,302,553 Δ1 bp coding (268/651 nt) alkB ← DNA oxidative demethylase AlkB
RA 2,303,411 T→C G158G (GGA→GGG ada ← bifunctional DNA‑binding transcriptional regulator/O6‑methylguanine‑DNA methyltransferase Ada
RA 2,306,184 T→C V15V (GTA→GTG ompC ← porin OmpC
RA 2,325,369 T→G pseudogene (2423/4515 nt) BW25113_RS11635 ← alpha‑2‑macroglobulin family protein
RA 2,345,762 A→G F31S (TTC→TCC)  glpT ← glycerol‑3‑phosphate transporter
RA 2,352,068 A→C V86G (GTC→GGC)  yfaU ← 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
RA 2,357,677 A→G G26G (GGT→GGC yfaZ ← YfaZ family protein
RA 2,370,881 A→G V64A (GTC→GCC)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 2,406,008 A→G D22D (GAT→GAC yfbU ← YfbU family protein
RA 2,438,026 T→C K69E (AAA→GAA)  epmC ← elongation factor P hydroxylase
RA 2,453,160 T→G K263N (AAA→AAC fadI ← acetyl‑CoA C‑acyltransferase FadI
RA 2,470,023 A→G intergenic (+34/+150) torI → / ← dsdC response regulator inhibitor TorI/DNA‑binding transcriptional regulator DsdC
RA 2,487,351 A→G D177D (GAT→GAC yfdX ← YfdX family protein
RA 2,499,179 C→T T311T (ACG→ACA ypdF ← aminopeptidase
RA 2,503,481 A→G T125A (ACC→GCC)  yfeO → ion channel protein
RA 2,521,647 C→T G630D (GGC→GAC)  ligA ← NAD‑dependent DNA ligase LigA
RA 2,525,250 T→A F143L (TTT→TTA cysZ → sulfate transporter CysZ
RA 2,537,187 T→C intergenic (‑300/+4) cysP ← / ← ucpA thiosulfate/sulfate ABC transporter substrate‑binding protein CysP/SDR family oxidoreductase UcpA
RA 2,550,584 A→G S25P (TCA→CCA)  eutC ← ethanolamine ammonia‑lyase subunit beta
RA 2,581,070 G→A A39A (GCG→GCA acrD → multidrug efflux RND transporter permease AcrD
RA 2,637,057 G→A L196F (CTT→TTT)  rlmN ← bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN
RA 2,638,870 T→C A605A (GCA→GCG pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,644,099 T→G A516A (GCA→GCC yfhM ← alpha2‑macroglobulin
RA 2,646,214 (G)6→7 coding (362/846 nt) sseA → 3‑mercaptopyruvate sulfurtransferase
RA 2,658,192 A→C Q148H (CAA→CAC yfhR → alpha/beta hydrolase
RA 2,666,371 T→A D322E (GAT→GAA hcaD → phenylpropionate dioxygenase ferredoxin reductase subunit
RA 2,682,977 T→C intergenic (‑112/+53) qseG ← / ← qseE two‑component system QseEF‑associated lipoprotein QseG/two component system sensor histidine kinase QseE/GlrK
RA 2,684,806 T→C intergenic (‑349/+209) qseE ← / ← purL two component system sensor histidine kinase QseE/GlrK/phosphoribosylformylglycinamidine synthase
RA 2,699,422 A→C R354R (CGT→CGG lepA ← translation elongation factor 4
RA 2,745,770 A→G E206G (GAG→GGG)  recN → DNA repair protein RecN
RA 2,772,143 A→G S1315P (TCC→CCC)  ypjA ← adhesin‑like autotransporter YpjA/EhaD
RA 2,813,426 A→G Y649H (TAT→CAT)  alaS ← alanine‑‑tRNA ligase
RA 2,829,895 A→G V297A (GTC→GCC)  hypF ← carbamoyltransferase HypF
RA 2,862,125 A→G intergenic (‑13/+127) nlpD ← / ← pcm murein hydrolase activator NlpD/protein‑L‑isoaspartate O‑methyltransferase
RA 2,890,306 T→A A332A (GCA→GCT ygcS ← MFS transporter
RA 2,891,751 T→G T343P (ACC→CCC)  ygcU ← FAD‑binding oxidoreductase
RA 2,931,836 A→G D337G (GAT→GGT)  fucK → L‑fuculokinase
RA 2,938,251 T→C D117D (GAT→GAC csdE → cysteine desulfurase sulfur acceptor subunit CsdE
RA 2,959,152 A→G F82S (TTC→TCC)  lgt ← prolipoprotein diacylglyceryl transferase
RA 3,005,362 T→C G181G (GGT→GGC arcC → carbamate kinase
RA 3,024,179 A→C F38V (TTT→GTT)  ygfT ← formate‑dependent uric acid utilization protein YgfT
RA 3,036,314 A→G F65S (TTC→TCC)  yqfA ← hemolysin III family protein
RA 3,071,778 A→C G147G (GGT→GGG cmtA ← PTS mannitol transporter subunit IICB
RA 3,148,355 A→G S56P (TCC→CCC)  yqhC ← DNA‑binding transcriptional regulator YqhC
RA 3,155,922 A→G V36A (GTT→GCT)  ftsP ← cell division protein FtsP
RA 3,184,461 T→C F157F (TTT→TTC yqiI → fimbrial protein
RA 3,193,900 A→G L142P (CTC→CCC)  ygiF ← inorganic triphosphatase
RA 3,202,275 T→G V298G (GTG→GGG)  ttdT → L‑tartrate/succinate antiporter
RA 3,210,830 A→C intergenic (+69/+85) nfeR → / ← aer DNA‑binding transcriptional regulator NfeR/aerotaxis sensor receptor Aer
RA 3,232,610 T→C I224I (ATT→ATC alx → TerC family membrane protein Alx
RA 3,292,953 +C coding (322/444 nt) yhbP ← YhbP family protein
RA 3,315,373 T→C intergenic (‑401/+58) yhbX ← / ← BW25113_RS16455 phosphoethanolamine transferase/tRNA‑Leu
RA 3,326,882 A→G intergenic (‑72/‑187) rplU ← / → ispB 50S ribosomal protein L21/octaprenyl diphosphate synthase
RA 3,349,491 T→C S470P (TCC→CCC)  gltB → glutamate synthase large subunit
RA 3,437,182 A→G I92T (ATC→ACC)  secY ← preprotein translocase subunit SecY
RA 3,446,256 A→C G10G (GGT→GGG rplC ← 50S ribosomal protein L3
RA 3,479,155 A→G V8A (GTC→GCC)  yhfA ← OsmC family protein
RA 3,516,705 T→C I159T (ATC→ACC)  mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,526,544 C→T A123V (GCT→GTT)  pckA → phosphoenolpyruvate carboxykinase (ATP)
RA 3,589,221 A→G G181G (GGT→GGC livH ← high‑affinity branched‑chain amino acid ABC transporter permease LivH
RA 3,593,877 T→C A89A (GCA→GCG rpoH ← RNA polymerase sigma factor RpoH
RA 3,602,485 A→G intergenic (‑129/‑92) tusA ← / → yhhQ sulfurtransferase TusA/7‑cyano‑7‑deazaguanine/7‑aminomethyl‑7‑ deazaguanine transporter
RA 3,617,742 A→G E2G (GAA→GGA)  yhhI → ISAs1‑like element ISEc1 family transposase
RA 3,836,431 A→G V240A (GTC→GCC)  adeQ ← adenine permease AdeQ
RA 3,841,593 A→G F131S (TTC→TCC)  uhpC ← MFS transporter family glucose‑6‑phosphate receptor UhpC
RA 3,853,103 A→G F125S (TTC→TCC)  yidK ← solute:sodium symporter family transporter
RA 3,873,583 A→G S333S (AGT→AGC recF ← DNA replication/repair protein RecF
RA 3,877,538 A→G intergenic (+72/‑158) BW25113_RS25780 → / → rpmH protein YsdD/50S ribosomal protein L34
RA 3,882,139 A→G E17G (GAG→GGG)  tnaA → tryptophanase
RA 3,896,219 A→G T281T (ACT→ACC bglB ← 6‑phospho‑beta‑glucosidase BglB
RA 3,901,162 T→C T189A (ACC→GCC)  pstB ← phosphate ABC transporter ATP‑binding protein PstB
RA 3,965,761 A→G D381G (GAC→GGC)  wecC → UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
RA 3,981,822 A→G I202T (ATC→ACC)  hemX ← uroporphyrinogen‑III C‑methyltransferase
RA 4,003,978 A→G E140G (GAG→GGG)  yigL → sugar/pyridoxal phosphate phosphatase YigL
RA 4,025,290 A→C D257A (GAC→GCC)  pepQ → Xaa‑Pro dipeptidase
RA 4,052,334 A→G I190V (ATT→GTT)  typA → ribosome‑dependent GTPase TypA
RA 4,057,690 A→T intergenic (‑35/+33) ompL ← / ← yihO porin OmpL/MFS transporter
RA 4,114,961 T→C E593G (GAA→GGA)  priA ← primosomal protein N'
RA 4,132,297 T→C intergenic (‑148/‑161) ptsP ← / → frwC phosphoenolpyruvate‑‑protein phosphotransferase/PTS fructose transporter subunit EIIC
RA 4,165,051 A→C intergenic (‑97/‑265) coaA ← / → BW25113_RS20630 type I pantothenate kinase/tRNA‑Thr
RA 4,167,337 A→G K18E (AAG→GAG)  secE → preprotein translocase subunit SecE
RA 4,172,602 A→C E477A (GAG→GCG)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,183,394 G→C G37G (GGC→GGG thiF ← thiazole biosynthesis adenylyltransferase ThiF
RA 4,208,503 T→A intergenic (+162/‑21) aceA → / → aceK isocitrate lyase/bifunctional isocitrate dehydrogenase kinase/phosphatase
RA 4,223,121 A→G V14A (GTA→GCA)  lysC ← lysine‑sensitive aspartokinase 3
RA 4,235,276 T→C N358D (AAC→GAC)  malE ← maltose/maltodextrin ABC transporter substrate‑binding protein MalE
RA 4,251,021 A→G pseudogene (773/986 nt) BW25113_RS21020 → DUF2713 domain‑containing protein
RA 4,262,930 A→T D290E (GAT→GAA uvrA ← excinuclease ABC subunit UvrA
RA 4,262,978 (C)5→4 coding (822/2823 nt) uvrA ← excinuclease ABC subunit UvrA
RA 4,264,031 A→T intergenic (‑232/‑22) uvrA ← / → ssb1 excinuclease ABC subunit UvrA/single‑stranded DNA‑binding protein SSB1
RA 4,321,803 T→C H495H (CAT→CAC proP → glycine betaine/L‑proline transporter ProP
RA 4,359,587 G→T R215S (CGT→AGT)  yjeH ← L‑methionine/branched‑chain amino acid transporter
RA 4,377,323 T→C D622G (GAC→GGC)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,378,349 T→C D280G (GAT→GGT)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,395,910 2 bp→CC intergenic (+108/‑96) purA → / → nsrR adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR
RA 4,409,650 T→C intergenic (‑208/‑147) ulaG ← / → ulaA L‑ascorbate 6‑phosphate lactonase/PTS ascorbate transporter subunit IIC
RA 4,428,964 T→C E39G (GAG→GGG)  ytfJ ← YtfJ family protein
RA 4,491,983 T→C R428R (AGA→AGG insG ← IS4‑like element IS4 family transposase
RA 4,493,312 A→G intergenic (‑46/‑563) insG ← / → nanX IS4‑like element IS4 family transposase/MFS transporter
RA 4,501,175 T→C I34V (ATC→GTC)  fecE ← Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE
RA 4,531,167 G→A A132T (GCC→ACC)  fimB → type 1 fimbria switch DNA invertase FimB
RA 4,545,596 T→C E181G (GAG→GGG)  yjiC ← YjfZ/YjiC family protein
RA 4,568,721 A→G I145T (ATC→ACC)  mcrB ← 5‑methylcytosine‑specific restriction endonuclease subunit McrB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357574 594 9 [0] [6] 9 [lacA] [lacA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)118 (2.960) 48/172 0.0 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =3 (0.080)52 (1.600) 33/140 0.0 95.0% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 3 (0.090)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032613 (0.080)31 (0.960) 19/140 0.4 91.9% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 3 (0.090)coding (57/540 nt) BW25113_RS06005 phage tail protein