breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,884 C→A P21Q (CCG→CAG)  folA → type 3 dihydrofolate reductase
RA 49,898 G→A A26T (GCC→ACC)  folA → type 3 dihydrofolate reductase
RA 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase
RA 59,627 C→A intergenic (‑153/+60) BW25113_RS25400 ← / ← rluA transposase/bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
RA 1,314,400 C→T A414A (GCG→GCA trpD ← bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 4,062,410 G→T P76Q (CCG→CAG)  yihQ ← sulfoquinovosidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357567 587 13 [0] [0] 7 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 2505243 2505288 46 3 [1] [0] 3 mntH Nramp family divalent metal transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 19796 =NA (NA)36 (1.040) 29/242 0.2 100% intergenic (‑1/+15) BW25113_RS25960/BW25113_RS00100 fimbrial family protein/IS1‑like element IS1A family transposase
?NZ_CP009273 3370648 = 0 (0.000)coding (132/639 nt) sspA stringent starvation protein A
* ? NZ_CP009273 360104 =154 (4.470)176 (5.100) 70/242 0.0 53.3% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =4 (0.120)19 (0.630) 16/210 0.5 81.8% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 5 (0.170)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032614 (0.120)21 (0.700) 18/210 0.4 83.2% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 5 (0.170)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 1972826 =1 (0.030)26 (0.750) 23/242 0.3 96.3% intergenic (‑372/‑408) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 3576788 = NA (NA)coding (391/417 nt) BW25113_RS17825 YrhA family protein
* ? NZ_CP009273 = 19728330 (0.000)23 (0.670) 20/242 0.4 100% intergenic (‑379/‑401) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 3577555 NA (NA)intergenic (+15/‑209) BW25113_RS17830/BW25113_RS25760 IS1‑like element IS1A family transposase/hypothetical protein
* ? NZ_CP009273 = 33706552 (0.060)45 (1.300) 38/242 0.0 95.7% coding (125/639 nt) sspA stringent starvation protein A
?NZ_CP009273 3576788 = NA (NA)coding (391/417 nt) BW25113_RS17825 YrhA family protein