breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 6,672 G→A L430F (CTC→TTC)  yaaJ ← sodium:alanine symporter family protein
RA 45,367 C→T S396S (AGC→AGT fixC → FAD‑dependent oxidoreductase
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,898 G→A A26T (GCC→ACC)  folA → type 3 dihydrofolate reductase
RA 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase
RA 59,627 C→A intergenic (‑153/+60) BW25113_RS25400 ← / ← rluA transposase/bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
RA 62,451 C→G A272P (GCC→CCC)  rapA ← RNA polymerase‑associated protein RapA
RA 85,430 G→A A306T (GCA→ACA)  cra → catabolite repressor/activator
RA 85,763 C→T intergenic (+244/‑358) cra → / → mraZ catabolite repressor/activator/division/cell wall cluster transcriptional repressor MraZ
RA 96,834 A→C Q235P (CAA→CCA)  murG → undecaprenyldiphospho‑muramoylpentapeptide beta‑N‑acetylglucosaminyltransferase
RA 100,067 A→G G142G (GGA→GGG ftsQ → cell division protein FtsQ
RA 103,795 G→A G251D (GGT→GAT)  lpxC → UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
RA 123,341 T→A L387Q (CTG→CAG)  aceF → pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase
RA 125,152 A→G M252V (ATG→GTG)  lpdA → dihydrolipoyl dehydrogenase
RA 149,183 A→G C39C (TGT→TGC yadM ← fimbrial protein
RA 183,409 A→G F577L (TTT→CTT)  glnD ← bifunctional uridylyltransferase/uridylyl‑removing protein GlnD
RA 188,059 G→T S239I (AGC→ATC)  tsf → translation elongation factor Ts
RA 189,045 A→T E235V (GAA→GTA)  pyrH → UMP kinase
RA 193,289 A→G H86R (CAC→CGC)  rseP → sigma E protease regulator RseP
RA 221,468 T→C noncoding (1211/1542 nt) BW25113_RS01010 → 16S ribosomal RNA
RA 232,843 T→A I97N (ATC→AAC)  dnaQ → DNA polymerase III subunit epsilon
RA 249,348 G→A E51E (GAG→GAA yafP → GNAT family N‑acetyltransferase
RA 251,770 T→C E145G (GAA→GGA)  pepD ← cytosol nonspecific dipeptidase
RA 288,667 A→G intergenic (‑8/+264) yagK ← / ← yagL inovirus Gp2 family protein/recombinase family protein
RA 306,481 (T)5→4 coding (519/543 nt) ecpR ← ECP biosynthesis operon DNA‑binding transcriptional regulator EcpR
RA 307,835 C→T P5S (CCG→TCG)  BW25113_RS01515 → hypothetical protein
RA 339,921 T→C M12T (ATG→ACG)  BW25113_RS01680 → hypothetical protein
RA 345,822 A→T T34S (ACC→TCC)  prpC → 2‑methylcitrate synthase
RA 362,870 G→T Q9H (CAG→CAT mhpA → bifunctional 3‑(3‑hydroxy‑phenyl)propionate/3‑hydroxycinnamic acid hydroxylase
RA 369,445 T→A V41E (GTG→GAG)  dmpG → 4‑hydroxy‑2‑oxovalerate aldolase
RA 381,199 A→G D171G (GAT→GGT)  tauA → taurine ABC transporter substrate‑binding protein
RA 400,441 T→C E220E (GAA→GAG proC ← pyrroline‑5‑carboxylate reductase
RA 409,497 T→C R571R (CGA→CGG sbcC ← exonuclease subunit SbcC
RA 412,148 G→A V87V (GTC→GTT sbcD ← exonuclease subunit SbcD
RA 436,326 C→T A78T (GCT→ACT)  ispA ← (2E,6E)‑farnesyl diphosphate synthase
RA 443,081 C→T L27L (CTG→CTA cyoE ← heme o synthase
RA 449,442 A→G V61A (GTC→GCC)  yajG ← lipoprotein
RA 481,749 G→A W178* (TGG→TAG)  acrR → multidrug efflux transporter transcriptional repressor AcrR
RA 484,563 G→A A858T (GCG→ACG)  mscK → mechanosensitive channel MscK
RA 493,309 (C)5→4 intergenic (+34/‑202) adk → / → hemH adenylate kinase/ferrochelatase
RA 518,671 (T)9→8 intergenic (+385/‑46) ybbP → / → rhsD ABC transporter permease/rhs element protein RhsD
RA 528,392 C→A intergenic (+3/‑75) allA → / → allR ureidoglycolate lyase/HTH‑type transcriptional repressor AllR
RA 543,592 C→T T486I (ACC→ATC)  fdrA → acyl‑CoA synthetase FdrA
RA 547,284 A→G I256T (ATC→ACC)  purK ← 5‑(carboxyamino)imidazole ribonucleotide synthase
RA 547,771 T→C T94A (ACT→GCT)  purK ← 5‑(carboxyamino)imidazole ribonucleotide synthase
RA 547,953 T→C D33G (GAC→GGC)  purK ← 5‑(carboxyamino)imidazole ribonucleotide synthase
RA 579,413 (T)6→5 coding (277/750 nt) appY → DNA‑binding transcriptional activator AppY
RA 585,704 T→A D236V (GAT→GTT)  nfrA ← bacteriophage adsorption protein NfrA
RA 599,729 C→T A131T (GCA→ACA)  ybdG ← mechanosensitive ion channel YbdG
RA 624,200 G→A R65Q (CGA→CAA)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase EntA
RA 628,572 G→A A121V (GCC→GTC)  hcxA ← hydroxycarboxylate dehydrogenase HcxA
RA 633,831 C→T A67T (GCT→ACT)  dsbG ← thiol:disulfide interchange protein DsbG
RA 635,912 G→A G235D (GGC→GAC)  ahpF → alkyl hydroperoxide reductase subunit F
RA 640,333 A→C W43G (TGG→GGG)  rna ← ribonuclease I
RA 683,163 G→A A14V (GCG→GTG)  gltI ← glutamate/aspartate ABC transporter substrate‑binding protein GltI
RA 714,441 A→T D492E (GAT→GAA speF ← ornithine decarboxylase SpeF
RA 721,666 A→G V276A (GTC→GCC)  kdpB ← potassium‑transporting ATPase subunit KdpB
RA 734,002 T→C pseudogene (455/759 nt) ybfD → ISAs1 family transposase
RA 753,637 A→G N165D (AAT→GAT)  sdhB → succinate dehydrogenase iron‑sulfur subunit SdhB
RA 783,406 T→C G296G (GGA→GGG galM ← galactose‑1‑epimerase
RA 797,386 T→C I15T (ATC→ACC)  ybhI → anion permease
RA 798,635 T→C G431G (GGT→GGC ybhI → anion permease
RA 803,049 G→A A106V (GCA→GTA)  ybhB ← kinase inhibitor
RA 861,119 G→A R234R (CGC→CGT moeA ← molybdopterin molybdotransferase MoeA
RA 869,360 T→C F309S (TTC→TCC)  pdeI → EAL domain‑containing protein
RA 883,821 C→T V485V (GTG→GTA ybjL ← aspartate:alanine antiporter
RA 918,237 T→A N14K (AAT→AAA yljB → protein YljB
RA 929,816 T→C G379G (GGT→GGC ftsK → DNA translocase FtsK
RA 937,543 A→C T377P (ACC→CCC)  dmsA → dimethylsulfoxide reductase subunit A
RA 958,894 G→A E482K (GAA→AAA)  rpsA → 30S ribosomal protein S1
RA 980,640 G→A L176L (CTG→TTG)  aspC ← aspartate transaminase
RA 1,006,907 T→C D496D (GAT→GAC uup → ABC transporter ATP‑binding protein
RA 1,017,704 C→A A549S (GCC→TCC)  yccS ← YccS family putative transporter
RA 1,043,563 A→C G195G (GGT→GGG gfcC ← capsule biosynthesis GfcC family protein
RA 1,047,923 (T)5→4 coding (896/1074 nt) yccM ← 4Fe‑4S binding protein
RA 1,049,216 A→G S807P (TCG→CCG)  torS ← TMAO reductase system sensor histidine kinase/response regulator TorS
RA 1,069,819 T→G L41R (CTG→CGG)  rutR → HTH‑type transcriptional regulator RutR
RA 1,070,975 G→A L1122F (CTC→TTC)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA 1,077,887 G→A K63K (AAG→AAA efeO → iron uptake system protein EfeO
RA 1,083,482 T→C Q611R (CAA→CGA)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase PgaB
RA 1,097,827 A→C G57G (GGT→GGG csgE ← curli production assembly/transport protein CsgE
RA 1,100,664 A→T D83V (GAT→GTT)  csgC → curli assembly chaperone CsgC
RA 1,119,962 T→C E274G (GAA→GGA)  mdtH ← multidrug efflux MFS transporter MdtH
RA 1,121,190 G→A G58D (GGC→GAC)  rimJ → ribosomal protein S5‑alanine N‑acetyltransferase
RA 1,134,272 A→G T147A (ACC→GCC)  flgK → flagellar hook‑associated protein FlgK
RA 1,158,853 Δ1 bp coding (138/825 nt) thiK → thiamine kinase
RA 1,196,042 A→G I74T (ATC→ACC)  intE ← site‑specific integrase
RA 1,199,864 G→A pseudogene (239/246 nt)
pseudogene (8/1137 nt)
BW25113_RS26105 →
BW25113_RS05965 →
helix‑turn‑helix domain‑containing protein
terminase large subunit
RA 1,213,874 Δ1 bp coding (1091/1524 nt) pdeG → cyclic diguanylate phosphodiesterase
RA 1,236,170 A→C L453R (CTT→CGT)  cvrA ← potassium/proton antiporter
RA 1,256,207 G→A A9V (GCT→GTT)  dauA ← C4‑dicarboxylic acid transporter DauA
RA 1,280,272 G→A A405T (GCA→ACA)  narH → nitrate reductase subunit beta
RA 1,284,091 (A)5→4 intergenic (‑11/+39) purU ← / ← ychJ formyltetrahydrofolate deformylase/YchJ family protein
RA 1,314,400 C→T A414A (GCG→GCA trpD ← bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
RA 1,350,506 G→T R288S (CGT→AGT)  sapA ← peptide ABC transporter substrate‑binding protein SapA
RA 1,367,307 T→C V381A (GTA→GCA)  ycjN → ABC transporter substrate‑binding protein
RA 1,373,111 G→A G324D (GGC→GAC)  ycjT → kojibiose phosphorylase
RA 1,386,326 C→T R27C (CGC→TGC)  pgrR → DNA‑binding transcriptional regulator PgrR
RA 1,401,680 G→A R125C (CGC→TGC)  dgcM ← diguanylate cyclase DgcM
RA 1,405,453 C→T P251P (CCG→CCA ttcA ← tRNA 2‑thiocytidine(32) synthetase TtcA
RA 1,412,718 A→C intergenic (‑232/‑210) kilR ← / → sieB killing protein KilR/superinfection exclusion B family protein
RA 1,506,942 T→G G344G (GGT→GGG ydcS → ABC transporter substrate‑binding protein
RA 1,513,670 G→A V129V (GTG→GTA curA → NADPH‑dependent curcumin/dihydrocurcumin reductase
RA 1,524,293 A→G intergenic (+98/‑105) BW25113_RS07645 → / → BW25113_RS26145 RHS repeat protein/hypothetical protein
RA 1,546,168 T→G W192G (TGG→GGG)  fdnI → formate dehydrogenase‑N subunit gamma
RA 1,547,080 G→A intergenic (‑141/+5) yddM ← / ← adhP HigA family addiction module antitoxin/alcohol dehydrogenase AdhP
RA 1,549,065 C→T A288T (GCG→ACG)  maeA ← malate dehydrogenase
RA 1,550,159 C→T R21H (CGC→CAC)  sra ← stationary‑phase‑induced ribosome‑associated protein
RA 1,561,943 C→T E380K (GAA→AAA)  digH ← glycoside hydrolase family 10 protein
RA 1,576,900 (A)6→5 intergenic (‑119/+283) ydeN ← / ← ydeO sulfatase/acid stress response transcriptional regulator YdeO
RA 1,579,503 A→G V414A (GTC→GCC)  ydeP ← acid resistance putative oxidoreductase YdeP
RA 1,581,105 A→G V296A (GTC→GCC)  ydeQ ← fimbrial protein
RA 1,582,843 A→G V85A (GTT→GCT)  ydeS ← fimbrial protein
RA 1,597,630 G→A V119I (GTT→ATT)  lsrC → autoinducer 2 ABC transporter permease LsrC
RA 1,603,097 A→C G61G (GGT→GGG yneE ← bestrophin family protein
RA 1,603,103 A→G I59I (ATT→ATC yneE ← bestrophin family protein
RA 1,605,384 T→C pseudogene (767/987 nt) dgcF ← GGDEF domain‑containing protein
RA 1,635,187 T→C P42P (CCT→CCC ynfU → putative zinc‑binding protein YnfU
RA 1,664,928 T→C S325P (TCG→CCG)  ynfM → MFS transporter
RA 1,718,009 A→G E544E (GAA→GAG ydhK → FUSC family protein
RA 1,744,804 T→C E156G (GAG→GGG)  ydhX ← 4Fe‑4S dicluster domain‑containing protein
RA 1,752,926 C→T Q19Q (CAG→CAA ldtE ← L,D‑transpeptidase LdtE
RA 1,785,565 T→C *238* (TAA→TGA)  ydiV ← anti‑FlhDC factor
RA 1,799,085 T→C pseudogene (1735/1900 nt) espL1 → type III secretion system effector EspL1
RA 1,809,775 A→T D551V (GAT→GTT)  katE → catalase HPII
RA 1,820,976 C→T A69T (GCG→ACG)  astE ← succinylglutamate desuccinylase
RA 1,891,283 A→G D32G (GAT→GGT)  sdaA → L‑serine ammonia‑lyase
RA 1,895,566 (C)6→7 coding (277/1557 nt) yoaE ← CNNM family cation transport protein YoaE
RA 1,913,346 T→G V527G (GTG→GGG)  yebT → lipid‑binding membrane homeostasis protein YebT
RA 1,927,446 A→G V472V (GTT→GTC edd ← phosphogluconate dehydratase
RA 1,977,975 C→T E192E (GAG→GAA araG ← arabinose ABC transporter ATP‑binding protein AraG
RA 2,014,277 A→G E237G (GAA→GGA)  fliM → flagellar motor switch protein FliM
RA 2,018,781 A→G intergenic (+87/‑211) yodD → / → yedP peroxide/acid resistance protein YodD/mannosyl‑3‑phosphoglycerate phosphatase‑related protein
RA 2,057,587 T→C E121G (GAG→GGG)  cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,068,298 A→C A13A (GCA→GCC BW25113_RS10450 → hypothetical protein
RA 2,069,296 A→G E346G (GAA→GGA)  BW25113_RS10450 → hypothetical protein
RA 2,099,575 A→G F378S (TTC→TCC)  wzy ← O16 family O‑antigen polymerase
RA 2,115,376 A→G L362P (CTG→CCG)  cpsG ← colanic acid biosynthesis phosphomannomutase CpsG
RA 2,121,319 G→A V159V (GTC→GTT gmd ← GDP‑mannose 4,6‑dehydratase
RA 2,124,319 G→A P14L (CCG→CTG)  wcaD ← colanic acid polymerase WcaD
RA 2,139,649 A→G E968G (GAA→GGA)  dgcE → diguanylate cyclase
RA 2,142,301 A→G T383A (ACG→GCG)  yegD → molecular chaperone
RA 2,146,283 C→T E109E (GAG→GAA yegL ← VWA domain‑containing protein
RA 2,158,576 C→T intergenic (+97/‑94) baeR → / → yegP two‑component system response regulator BaeR/YegP family protein
RA 2,176,075 A→G I62I (ATT→ATC yegW ← GntR family transcriptional regulator
RA 2,190,509 T→C pseudogene (557/3796 nt) BW25113_RS25680 → WGR and DUF4132 domain‑containing protein
RA 2,217,891 C→A S153S (TCG→TCT pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,245,489 C→T A104V (GCG→GTG)  yeiI → sugar kinase
RA 2,250,936 G→A A304V (GCC→GTC)  psuG ← pseudouridine‑5'‑phosphate glycosidase
RA 2,328,749 A→G S459P (TCC→CCC)  yfaA ← DUF2138 domain‑containing protein
RA 2,351,422 A→C pseudogene (140/198 nt) BW25113_RS11725 → hypothetical protein
RA 2,368,479 T→C I2V (ATC→GTC) 
*321W (TGA→TGG
menE ←
menC ←
o‑succinylbenzoate‑‑CoA ligase
o‑succinylbenzoate synthase
RA 2,370,899 A→T V58D (GTC→GAC)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,371,693 A→G I349T (ATC→ACC)  menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase
RA 2,376,303 (A)8→7 coding (112/1212 nt) elaD → deubiquitinase ElaD
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 2,400,959 A→C intergenic (‑839/‑81) lrhA ← / → alaA transcriptional regulator LrhA/alanine transaminase AlaA
RA 2,401,096 T→C G19G (GGT→GGC alaA → alanine transaminase AlaA
RA 2,441,796 A→C W42G (TGG→GGG)  prmB ← 50S ribosomal protein L3 N(5)‑glutamine methyltransferase
RA 2,448,105 G→A pseudogene (376/2646 nt) yfcU ← fimbrial usher protein YfcU
RA 2,451,709 G→T S310R (AGC→AGA fadJ ← fatty acid oxidation complex subunit alpha FadJ
RA 2,453,267 T→C T228A (ACT→GCT)  fadI ← acetyl‑CoA C‑acyltransferase FadI
RA 2,464,440 T→C pseudogene (147/291 nt) BW25113_RS12275 → tail fiber assembly protein
RA 2,484,113 A→G S439S (TCT→TCC oxc ← oxalyl‑CoA decarboxylase
RA 2,497,341 (C)5→4 coding (624/2496 nt) ptsP ← phosphoenolpyruvate‑‑protein phosphotransferase
RA 2,519,318 G→C R300G (CGG→GGG)  yfeR ← LysR family transcriptional regulator
RA 2,521,249 A→C K315N (AAA→AAC yfeH → bile acid:sodium symporter
RA 2,557,468 A→T N44Y (AAC→TAC)  BW25113_RS12770 → hypothetical protein
RA 2,569,373 A→G intergenic (‑209/+84) eutS ← / ← maeB ethanolamine utilization microcompartment protein EutS/NADP‑dependent oxaloacetate‑decarboxylating malate dehydrogenase
RA 2,575,962 T→C S59G (AGC→GGC)  ypfG ← DUF1176 domain‑containing protein
RA 2,580,168 A→G E360G (GAA→GGA)  narQ → nitrate/nitrite two‑component system sensor histidine kinase NarQ
RA 2,590,869 Δ1 bp coding (109/714 nt) purC ← phosphoribosylaminoimidazolesuccinocarboxamide synthase
RA 2,595,868 T→C L231S (TTG→TCG)  hyfB → hydrogenase 4 subunit B
RA 2,625,832 T→C Q21Q (CAA→CAG guaA ← glutamine‑hydrolyzing GMP synthase
RA 2,631,737 A→G I92T (ATC→ACC)  bamB ← outer membrane protein assembly factor BamB
RA 2,636,307 A→G intergenic (‑104/+181) rodZ ← / ← rlmN cytoskeleton protein RodZ/bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN
RA 2,667,151 A→G intergenic (+24/+24) yphA → / ← yphB DoxX family protein/aldose 1‑epimerase
RA 2,700,943 C→T S73N (AGC→AAC)  rseC ← SoxR‑reducing system protein RseC
RA 2,705,165 G→A A463T (GCC→ACC)  nadB → L‑aspartate oxidase
RA 2,716,348 A→G D88G (GAC→GGC)  pssA → CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
RA 2,718,722 A→G L128L (CTT→CTC kgtP ← alpha‑ketoglutarate permease
RA 2,718,745 T→G T121P (ACG→CCG)  kgtP ← alpha‑ketoglutarate permease
RA 2,724,669 T→G intergenic (‑153/+290) BW25113_RS13535 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA 2,724,674 T→C intergenic (‑158/+285) BW25113_RS13535 ← / ← clpB 16S ribosomal RNA/ATP‑dependent chaperone ClpB
RA 2,727,607 A→G intergenic (‑75/+55) clpB ← / ← pgeF ATP‑dependent chaperone ClpB/purine nucleoside phosphorylase YfiH
RA 2,732,532 A→G D299D (GAT→GAC tyrA ← bifunctional chorismate mutase/prephenate dehydrogenase
RA 2,761,884 T→C R272R (CGT→CGC yfjP → GTPase family protein
RA 2,762,893 T→C intergenic (+48/‑169) yfjQ → / → yfjR DUF932 domain‑containing protein/WYL domain‑containing protein
RA 2,775,752 (C)5→4 coding (334/4581 nt) ypjA ← adhesin‑like autotransporter YpjA/EhaD
RA 2,778,322 T→A pseudogene (49/1374 nt) BW25113_RS25705 ← DUF5507 domain‑containing protein
RA 2,781,609 T→C pseudogene (1854/2253 nt) BW25113_RS26005 → alpha‑amylase family glycosyl hydrolase
RA 2,867,513 A→G D422D (GAT→GAC cysN ← sulfate adenylyltransferase subunit CysN
RA 2,894,306 T→G V119G (GTG→GGG)  yqcE → MFS transporter
RA 2,897,407 A→C intergenic (+674/+699) ygcE → / ← queE FGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
RA 2,901,362 A→G intergenic (‑62/+26) eno ← / ← pyrG phosphopyruvate hydratase/CTP synthase (glutamine hydrolyzing)
RA 2,909,267 G→A K284K (AAG→AAA barA → two‑component sensor histidine kinase BarA
RA 2,910,284 A→G K623K (AAA→AAG barA → two‑component sensor histidine kinase BarA
RA 2,919,491 A→G D262G (GAC→GGC)  queF → NADPH‑dependent 7‑cyano‑7‑deazaguanine reductase QueF
RA 2,923,200 A→G D89G (GAT→GGT)  sdaB → L‑serine ammonia‑lyase II
RA 2,932,567 T→C R107R (CGT→CGC fucU → L‑fucose mutarotase
RA 2,972,598 A→G R73R (CGA→CGG lysR → DNA‑binding transcriptional regulator LysR
RA 2,976,301 T→C E106G (GAA→GGA)  kduD ← 2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase KduD
RA 2,982,682 (A)8→7 coding (20/483 nt) BW25113_RS14815 → hypothetical protein
RA 2,985,850 A→G D133G (GAT→GGT)  ygeH → HilA family transcriptional regulator YgeH
RA 2,999,490 T→C intergenic (‑345/‑134) ygeV ← / → ygeW sigma‑54‑dependent Fis family transcriptional regulator/knotted carbamoyltransferase YgeW
RA 2,999,765 C→A R48S (CGT→AGT)  ygeW → knotted carbamoyltransferase YgeW
RA 3,005,890 C→A intergenic (+138/+83) arcC → / ← yqeB carbamate kinase/selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA 3,015,364 T→C V230V (GTT→GTC xdhD → molybdopterin‑dependent oxidoreductase Mo/Fe‑S‑binding subunit
RA 3,034,841 A→C H57P (CAC→CCC)  ygfZ → tRNA‑modifying protein YgfZ
RA 3,050,507 A→G intergenic (‑199/+30) ibsC ← / ← serA type I toxin‑antitoxin system toxin IbsC/phosphoglycerate dehydrogenase
RA 3,063,057 A→T I37N (ATC→AAC)  mscS ← small‑conductance mechanosensitive channel MscS
RA 3,082,721 T→C V360A (GTC→GCC)  galP → galactose/proton symporter
RA 3,085,483 A→T I83F (ATC→TTC)  gshB → glutathione synthase
RA 3,086,782 G→A A163T (GCC→ACC)  yqgE → YqgE/AlgH family protein
RA 3,095,990 (C)5→4 coding (222/720 nt) trmB ← tRNA (guanosine(46)‑N7)‑methyltransferase TrmB
RA 3,100,032 C→T C320C (TGC→TGT nupG → nucleoside permease NupG
RA 3,168,157 A→T I200N (ATC→AAC)  parE ← DNA topoisomerase IV subunit B
RA 3,223,174 A→T I310F (ATC→TTC)  ygjK → alpha‑glucosidase
RA 3,266,228 C→T G230D (GGC→GAC)  garL ← 2‑dehydro‑3‑deoxyglucarate aldolase
RA 3,286,821 C→T A21A (GCC→GCT lpoA → penicillin‑binding protein activator LpoA
RA 3,297,285 G→T G160V (GGC→GTC)  yhbW → luciferase‑like monooxygenase
RA 3,300,885 C→T G112D (GGC→GAC)  deaD ← ATP‑dependent RNA helicase DeaD
RA 3,310,402 T→A I162F (ATC→TTC)  nusA ← transcription termination factor NusA
RA 3,312,159 C→T A55V (GCG→GTG)  argG → argininosuccinate synthase
RA 3,353,444 C→A G296G (GGC→GGA gltD → glutamate synthase subunit GltD
RA 3,357,838 T→C V558A (GTC→GCC)  yhcD → fimbrial biogenesis outer membrane usher protein
RA 3,366,821 T→C I39V (ATC→GTC)  nanA ← N‑acetylneuraminate lyase
RA 3,394,698 T→C intergenic (‑252/+53) mreB ← / ← csrD rod shape‑determining protein MreB/RNase E specificity factor CsrD
RA 3,403,270 A→G E281G (GAA→GGA)  prmA → 50S ribosomal protein L11 methyltransferase
RA 3,413,569 T→C I26T (ATC→ACC)  yhdX → amino acid ABC transporter permease
RA 3,429,447 T→G G294G (GGT→GGG rsmB → 16S rRNA (cytosine(967)‑C(5))‑methyltransferase RsmB
RA 3,452,746 G→T G350V (GGC→GTC)  gspE → type II secretion system ATPase GspE
RA 3,460,220 Δ1 bp coding (131/195 nt) bfd ← bacterioferritin‑associated ferredoxin
RA 3,467,417 A→G pseudogene (24/468 nt) rpsG ← 30S ribosomal protein S7
RA 3,486,387 T→C F154S (TTT→TCT)  tsgA → MFS transporter TsgA
RA 3,486,985 A→C E353D (GAA→GAC tsgA → MFS transporter TsgA
RA 3,501,576 T→C E125G (GAG→GGG)  php ← phosphotriesterase‑related protein
RA 3,517,393 T→C D388D (GAT→GAC mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,521,284 A→G D486G (GAT→GGT)  igaA → intracellular growth attenuator protein IgaA
RA 3,539,325 C→T L115L (CTG→TTG)  nfuA → Fe‑S biogenesis protein NfuA
RA 3,540,121 C→T G68G (GGC→GGT gntT → gluconate transporter
RA 3,543,436 T→C intergenic (‑7/+3) malQ ← / ← malP 4‑alpha‑glucanotransferase/maltodextrin phosphorylase
RA 3,562,031 C→A A220S (GCC→TCC)  glgC ← glucose‑1‑phosphate adenylyltransferase
RA 3,576,651 T→C I85T (ATT→ACT)  BW25113_RS17825 → YrhA family protein
RA 3,595,866 A→G I81T (ATT→ACT)  ftsE ← cell division ATP‑binding protein FtsE
RA 3,637,299 T→C E415G (GAA→GGA)  prlC ← oligopeptidase A
RA 3,655,663 G→A K630K (AAG→AAA mdtF → multidrug efflux pump RND permease MdtF
RA 3,660,392 Δ1 bp coding (549/1401 nt) gadA ← glutamate decarboxylase
RA 3,674,379 G→T H308N (CAT→AAT)  yhjJ ← pitrilysin family protein
RA 3,705,327 A→G L14P (CTC→CCC)  yhjX ← MFS transporter
RA 3,706,510 A→G E20G (GAG→GGG)  tag → DNA‑3‑methyladenine glycosylase I
RA 3,708,729 C→T T342T (ACG→ACA bisC ← biotin sulfoxide reductase
RA 3,710,430 C→T A175V (GCG→GTG)  yiaD → OmpA family lipoprotein
RA 3,724,067 T→C N20S (AAC→AGC)  xylA ← xylose isomerase
RA 3,750,235 T→C E318G (GAA→GGA)  yiaY ← L‑threonine dehydrogenase
RA 3,753,672 G→A A313V (GCC→GTC)  selA ← L‑seryl‑tRNA(Sec) selenium transferase
RA 3,766,062 T→C I141T (ATC→ACC)  mtlA → PTS mannitol transporter subunit IICBA
RA 3,781,002 A→G T269A (ACG→GCG)  envC → murein hydrolase activator EnvC
RA 3,805,353 T→C E189E (GAA→GAG radC ← DNA repair protein RadC
RA 3,816,198 C→T L147L (CTG→TTG)  spoT → bifunctional GTP diphosphokinase/guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,828,209 T→A I361F (ATC→TTC)  yicJ ← glycoside‑pentoside‑hexuronide family transporter
RA 3,829,894 (C)6→5 pseudogene (140/162 nt) BW25113_RS26245 → virulence RhuM family protein
RA 3,845,367 T→C M260V (ATG→GTG)  ilvB ← acetolactate synthase large subunit
RA 3,845,459 A→C V229G (GTG→GGG)  ilvB ← acetolactate synthase large subunit
RA 3,853,538 T→C intergenic (‑62/‑105) yidK ← / → yidL solute:sodium symporter family transporter/AraC family transcriptional regulator
RA 3,858,886 T→C T250A (ACC→GCC)  yidE ← putative transporter
RA 3,883,081 T→G L331R (CTG→CGG)  tnaA → tryptophanase
RA 3,913,243 A→C V174G (GTC→GGC)  atpH ← F0F1 ATP synthase subunit delta
RA 3,914,592 T→C intergenic (‑43/+4) atpE ← / ← atpB F0F1 ATP synthase subunit C/F0F1 ATP synthase subunit A
RA 3,931,799 T→C N71N (AAT→AAC rbsR → ribose operon transcriptional repressor RbsR
RA 3,935,427 +G noncoding (260/1542 nt) BW25113_RS19520 → 16S ribosomal RNA
RA 3,946,052 T→C F70S (TTC→TCC)  ilvE → branched‑chain‑amino‑acid transaminase
RA 3,961,670 T→G V132G (GTG→GGG)  wecA → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 3,994,324 A→G V31V (GTT→GTC yigE ← phosphodiester glycosidase family protein
RA 3,994,362 T→C I19V (ATT→GTT)  yigE ← phosphodiester glycosidase family protein
RA 3,994,419 T→C intergenic (‑3/‑159) yigE ← / → ysgD phosphodiester glycosidase family protein/protein YsgD
RA 3,995,690 C→T A302V (GCG→GTG)  corA → magnesium/cobalt transporter CorA
RA 4,001,645 C→T R177C (CGC→TGC)  rhtC → threonine export protein RhtC
RA 4,016,642 A→C I183L (ATT→CTT)  tatC → Sec‑independent protein translocase subunit TatC
RA 4,038,395 T→C N279N (AAT→AAC yihF → YdgA family protein
RA 4,041,206 G→A G294D (GGC→GAC)  polA → DNA polymerase I
RA 4,062,410 G→T P76Q (CCG→CAG)  yihQ ← sulfoquinovosidase
RA 4,064,803 T→C D105G (GAT→GGT)  yihS ← sulfoquinovose isomerase
RA 4,077,063 T→A D707V (GAT→GTT)  fdnG ← formate dehydrogenase‑N subunit alpha
RA 4,078,866 C→T S106N (AGC→AAC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA 4,078,948 C→A E79* (GAA→TAA)  fdnG ← formate dehydrogenase‑N subunit alpha
RA 4,081,683 C→T E511E (GAG→GAA frvR ← putative frv operon regulatory protein
RA 4,140,022 T→C I58V (ATC→GTC)  eptC ← phosphoethanolamine transferase CptA
RA 4,141,321 (T)5→4 coding (1706/2652 nt) ppc ← phosphoenolpyruvate carboxylase
RA 4,222,007 A→C G385G (GGT→GGG lysC ← lysine‑sensitive aspartokinase 3
RA 4,257,469 T→C F143S (TTC→TCC)  tyrB → aromatic amino acid transaminase
RA 4,263,842 T→G intergenic (‑43/‑211) uvrA ← / → ssb1 excinuclease ABC subunit UvrA/single‑stranded DNA‑binding protein SSB1
RA 4,272,855 A→G L50P (CTC→CCC)  espX5 ← T3SS effector pentapeptide repeat protein EspX5
RA 4,324,872 T→G Y179S (TAC→TCC)  eptA ← phosphoethanolamine transferase EptA
RA 4,359,148 G→A A361V (GCA→GTA)  yjeH ← L‑methionine/branched‑chain amino acid transporter
RA 4,443,731 C→T A111V (GCC→GTC)  yjfF → sugar ABC transporter permease YjfF
RA 4,445,051 T→G T126P (ACC→CCC)  fbp ← class 1 fructose‑bisphosphatase
RA 4,469,838 C→T L98F (CTT→TTT)  yjgN → YjgN family protein
RA 4,516,576 A→G Y44H (TAT→CAT)  sgcR ← DeoR/GlpR family DNA‑binding transcription regulator
RA 4,525,950 T→A intergenic (+102/+166) yjhR → / ← BW25113_RS26295 helicase YjhR/HNH endonuclease
RA 4,527,329 A→G L28P (CTG→CCG)  nanS ← 9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
RA 4,528,751 T→C T190A (ACG→GCG)  nanC ← N‑acetylneuraminic acid outer membrane channel NanC
RA 4,534,382 C→T P88S (CCT→TCT)  fimC → type 1 fimbria chaperone FimC
RA 4,585,319 Δ1 bp coding (350/915 nt) lgoR ← GntR family transcriptional regulator
RA 4,616,195 A→G E156G (GAA→GGA)  radA → DNA repair protein RadA
RA 4,616,725 C→T L333L (CTG→TTG)  radA → DNA repair protein RadA
RA 4,630,135 A→G intergenic (‑12/‑84) arcA ← / → BW25113_RS22930 two‑component system response regulator ArcA/hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 295786 295852 67 15 [14] [14] 15 paoC aldehyde oxidoreductase molybdenum‑binding subunit PaoC
* * ÷ NZ_CP009273 356947 357592 646 19 [13] [12] 15 lacA lacA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =74 (1.160)99 (1.550) 50/240 0.1 57.2% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =6 (0.090)45 (0.810) 33/208 0.3 90.7% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 4 (0.070)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032616 (0.090)45 (0.810) 35/208 0.3 90.7% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 4 (0.070)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 1390700 =NA (NA)47 (0.740) 32/240 0.6 50.5% coding (332/981 nt) BW25113_RS06980 IS5‑like element IS5 family transposase
?NZ_CP009273 1422137 = 46 (0.720)coding (847/981 nt) BW25113_RS07180 IS5‑like element ISKpn26 family transposase