breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,903 T→G D27E (GAT→GAG folA → type 3 dihydrofolate reductase
RA 49,905 T→G L28R (CTC→CGC)  folA → type 3 dihydrofolate reductase
RA 50,279 T→G F153V (TTT→GTT)  folA → type 3 dihydrofolate reductase
RA 59,627 C→A intergenic (‑153/+60) BW25113_RS25400 ← / ← rluA transposase/bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
RA 1,314,400 C→T A414A (GCG→GCA trpD ← bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 4,015,411 T→C I36T (ATC→ACC)  tatA → Sec‑independent protein translocase subunit TatA
RA 4,062,410 G→T P76Q (CCG→CAG)  yihQ ← sulfoquinovosidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357576 596 8 [0] [2] 3 [lacA] [lacA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =22 (0.660)31 (0.940) 20/246 0.4 58.5% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =24 (0.720)8 (0.280) 8/214 1.3 30.8% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 15 (0.520)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391495NA (NA)21 (0.630) 17/246 0.6 100% intergenic (+146/‑127) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 = 1972751 0 (0.000)intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 1972747 =0 (0.000)25 (0.760) 17/244 0.6 100% intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283591 NA (NA)intergenic (‑67/‑388) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP