breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 50,102 A→C I94L (ATT→CTT)  folA → type 3 dihydrofolate reductase
RA 1,858,499 G→C W131C (TGG→TGC yeaD → D‑hexose‑6‑phosphate mutarotase
RA 2,013,868 A→G N101D (AAC→GAC)  fliM → flagellar motor switch protein FliM
RA 2,236,491 A→C F214C (TTT→TGT)  folE ← GTP cyclohydrolase I FolE
JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 4,171,725 G→C D185H (GAC→CAC)  rpoB → DNA‑directed RNA polymerase subunit beta

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356970 357574 605 12 [11] [10] 13 [lacA] [lacA]
* * ÷ NZ_CP009273 3629063 3646526–3645391 16329–17464 149 [0] [11] 13 [yhiM]–BW25113_RS18160 [yhiM],yhiN,pitA,uspB,uspA,BW25113_RS26225,BW25113_RS26230,dtpB,rsmJ,prlC,rlmJ,gorA,dinQ,arsR,arsB,arsC,BW25113_RS18155,BW25113_RS18160

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)71 (0.910) 29/160 0.8 45.8% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 84 (1.070)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =14 (0.180)57 (0.910) 34/128 0.3 82.5% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 13 (0.210)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326114 (0.180)26 (0.420) 18/128 1.2 68.3% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 13 (0.210)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 1972818 =3 (0.040)92 (1.180) 50/160 0.1 96.8% intergenic (‑364/‑416) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2096424 NA (NA)intergenic (‑68/+3) BW25113_RS10605/wbbL IS5‑like element IS5 family transposase/N‑acetylglucosaminyl‑diphospho‑decaprenol L‑rhamnosyltransferase WbbL
* ? NZ_CP009273 = 19728212 (0.030)68 (0.870) 36/160 0.5 97.1% intergenic (‑367/‑413) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 2095230 = NA (NA)pseudogene (1/354 nt) wbbL N‑acetylglucosaminyl‑diphospho‑decaprenol L‑rhamnosyltransferase WbbL
* ? NZ_CP009273 2282398 =NA (NA)148 (1.890) 56/160 0.1 100% pseudogene (3/2532 nt) yejO autotransporter YejO
?NZ_CP009273 = 3629062 0 (0.000)coding (862/1053 nt) yhiM DUF2776 domain‑containing protein
* ? NZ_CP009273 4532499 =9 (0.120)73 (1.050) 34/142 0.4 82.5% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 23 (0.330)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 45325079 (0.120)69 (1.000) 35/142 0.4 81.7% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 23 (0.330)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA