breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,903 T→A D27E (GAT→GAA folA → type 3 dihydrofolate reductase
RA 49,910 T→A W30R (TGG→AGG)  folA → type 3 dihydrofolate reductase
JC 1,613,631 Δ3 bp coding (255‑257/435 nt) marR → multiple antibiotic resistance transcriptional regulator MarR
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,632,461 T→G L487R (CTT→CGT)  pitA → inorganic phosphate transporter PitA
RA 3,754,231 G→T R127S (CGC→AGC)  selA ← L‑seryl‑tRNA(Sec) selenium transferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357567 587 4 [0] [0] 4 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 579014 579053 40 2 [1] [0] 2 BW25113_RS26085/appY tail fiber assembly protein/DNA‑binding transcriptional activator AppY
* * ÷ NZ_CP009273 656818 656829 12 2 [0] [1] 3 ybeF YbeF family transcriptional regulator
* * ÷ NZ_CP009273 716424 716481 58 2 [1] [1] 2 BW25113_RS26090/kdpE leader peptide SpeFL/two‑component system response regulator KdpE
* * ÷ NZ_CP009273 1091297 1091306 10 2 [0] [1] 2 [BW25113_RS05355]–[BW25113_RS05360] [BW25113_RS05355],[BW25113_RS05360]
* * ÷ NZ_CP009273 1164362 1164427 66 2 [1] [1] 3 comR/bhsA TetR family copper‑responsive transcriptional repressor ComR/multiple stress resistance protein BhsA
* * ÷ NZ_CP009273 1192452 1192489 38 2 [1] [1] 2 ymfD class I SAM‑dependent methyltransferase
* * ÷ NZ_CP009273 1193084 1193144 61 3 [0] [0] 2 BW25113_RS05920 hypothetical protein
* * ÷ NZ_CP009273 1193262 1193312 51 2 [0] [1] 2 BW25113_RS05920 hypothetical protein
* * ÷ NZ_CP009273 1206767 1206774 8 2 [0] [1] 2 mcrA/BW25113_RS23640 5‑methylcytosine‑specific endonuclease McrA/isocitrate/isopropylmalate family dehydrogenase
* * ÷ NZ_CP009273 1807468 1807552 85 2 [1] [1] 2 BW25113_RS09050/cedA hypothetical protein/cell division activator CedA
* * ÷ NZ_CP009273 2027104 2027124 21 2 [1] [0] 2 drpB/ompC cell division protein DrpB/porin OmpC
* * ÷ NZ_CP009273 2462910 2462944 35 3 [1] [1] 2 BW25113_RS12270 hypothetical protein
* * ÷ NZ_CP009273 2463141 2463180 40 2 [1] [0] 2 BW25113_RS12270 hypothetical protein
* * ÷ NZ_CP009273 2463618 2463665 48 2 [1] [0] 2 BW25113_RS12270 hypothetical protein
* * ÷ NZ_CP009273 2877661 2877688 28 2 [1] [1] 2 casA/cas3 type I‑E CRISPR‑associated protein Cse1/CasA/CRISPR‑associated helicase/endonuclease Cas3

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)100 (3.350) 33/164 0.0 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =7 (0.230)16 (0.670) 9/132 0.8 78.8% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 3 (0.120)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032617 (0.230)17 (0.710) 12/132 0.5 79.8% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 3 (0.120)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)19 (0.640) 11/162 0.8 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)28 (0.940) 22/164 0.2 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 4532499 =2 (0.070)10 (0.380) 6/146 1.4 84.1% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 2 (0.080)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 45325072 (0.070)5 (0.190) 5/146 1.6 72.6% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 2 (0.080)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA