breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 702,330 Δ1 bp 22.2% coding (422/1149 nt) nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 1,158,383 G→A 13.7% W172* (TGG→TAG)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
JC JC 1,159,287 IS2 (–) +5 bp 60.2% coding (1419‑1423/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,466,438 T→G 5.9% pseudogene (1047/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,712 G→A 5.8% pseudogene (1321/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,714 T→C 6.6% pseudogene (1323/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,717 C→T 8.7% pseudogene (1326/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,729 G→A 6.4% pseudogene (1338/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,708,709 T→C 9.5% R597R (CGT→CGC rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,726,155 T→G 55.2% F45V (TTC→GTC)  nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 1,726,373 Δ1 bp 16.7% coding (351/600 nt) nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 1,726,374 Δ1 bp 16.7% coding (352/600 nt) nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
JC 1,726,492 Δ4 bp 17.6% coding (470‑473/600 nt) nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 1,726,528 G→T 7.4% G169V (GGC→GTC)  nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 1,726,570:1 +G 7.5% coding (548/600 nt) nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,233,246 T→G 10.4% F528V (TTC→GTC)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,875,529 C→G 6.3% S31C (TCC→TGC)  yidX → putative lipoprotein
RA 3,875,536 C→A 8.8% T33T (ACC→ACA yidX → putative lipoprotein
MC JC 3,909,312 Δ774 bp 100% [pstA][pstC] [pstA], [pstC]
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,171,648 A→C 100% noncoding (12/120 nt) rrfB → 5S ribosomal RNA of rrnB operon
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,060 C→T 6.9% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→G 26.0% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 23.2% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 23.4% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,326,088 T→G 6.2% intergenic (‑347/+311) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
JC 4,606,167 Δ236 bp 5.1% leuPleuQ leuP, leuQ

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4110687 4111551 865 63 [61] [61] 63 tpiA tpiA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 225456 =70 (0.480)5 (0.040) 5/502 NT 5.7% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 4037237 = 97 (0.700)intergenic (+20/‑23) ileT/alaT tRNA‑Ile/tRNA‑Ala
* ? NC_000913 228016 =NA (NA)3 (0.020) 3/506 NT NA noncoding (2258/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 228041 = NA (NA)noncoding (2283/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 1207790 =53 (0.370)83 (0.610) 60/488 NT 64.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 42 (0.310)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120779455 (0.380)3 (0.020)
+G
3/518 NT 5.8% coding (294/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209612 43 (0.300)pseudogene (8/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780553 (0.370)86 (0.630) 57/488 NT 65.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 42 (0.310)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)81 (0.570) 61/512 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein