breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 44,865 | T→A | 21.0% | intergenic (‑46/‑455) | abrB ← / → metG | transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase |
RA | 44,870 | C→T | 21.0% | intergenic (‑51/‑450) | abrB ← / → metG | transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase |
RA | 44,871 | G→C | 20.9% | intergenic (‑52/‑449) | abrB ← / → metG | transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase |
RA | 44,881 | G→A | 76.7% | intergenic (‑62/‑439) | abrB ← / → metG | transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase |
RA | 132,099 | T→C | 62.4% | V531A (GTA→GCA) | fusA → | elongation factor G |
RA | 132,185 | G→A | 24.2% | D560N (GAC→AAC) | fusA → | elongation factor G |
RA | 135,117 | G→T | 6.5% | intergenic (+164/‑74) | EO946_RS00715 → / → rpsJ | alpha/beta hydrolase/30S ribosomal protein S10 |
RA | 209,812 | G→T | 5.3% | A234S (GCA→TCA) | EO946_RS01190 → | alpha/beta hydrolase |
RA | 240,211 | G→T | 11.6% | A107A (GCC→GCA) | EO946_RS01360 ← | hypothetical protein |
RA | 316,262 | C→A | 8.2% | Q28K (CAA→AAA) | opuAA → | glycine/proline betaine ABC transporter ATP‑binding protein OpuAA |
RA | 399,035 | C→A | 8.2% | V949V (GTC→GTA) | srfAC → | surfactin non‑ribosomal peptide synthetase SrfAC |
RA | 607,359 | A→C | 13.5% | T68P (ACC→CCC) | EO946_RS03185 → | collagen‑like protein |
JC | 607,424 | 27 bp→108 bp | 47.1% | coding (267‑293/1125 nt) | EO946_RS03185 → | collagen‑like protein |
RA | 607,529 | G→C | 6.4% | A124A (GCG→GCC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,562 | G→A | 6.3% | G135G (GGG→GGA) | EO946_RS03185 → | collagen‑like protein |
RA | 607,565 | C→G | 9.8% | A136A (GCC→GCG) | EO946_RS03185 → | collagen‑like protein |
RA | 607,638 | G→C | 9.5% | A161P (GCC→CCC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,700 | A→C | 16.7% | E181D (GAA→GAC) | EO946_RS03185 → | collagen‑like protein |
JC | 607,746 | Δ45 bp | 8.4% | coding (589‑633/1125 nt) | EO946_RS03185 → | collagen‑like protein |
RA | 607,838 | A→G | 8.8% | T227T (ACA→ACG) | EO946_RS03185 → | collagen‑like protein |
RA | 634,086 | G→T | 6.2% | S562* (TCA→TAA) | EO946_RS03330 ← | ABC‑F family ATP‑binding cassette domain‑containing protein |
RA | 814,356 | T→G | 8.8% | H340Q (CAT→CAG) | nosA → | nitric oxide synthase |
RA | 886,747 | A→C | 10.5% | P47P (CCT→CCG) | EO946_RS04490 ← | MFS transporter |
RA | 924,135 | A→T | 5.4% | intergenic (+10/+41) | EO946_RS04760 → / ← spo0M | tRNA‑Leu/sporulation control protein Spo0M |
RA | 986,801 | G→T | 10.3% | D372Y (GAT→TAT) | spoVR → | stage V sporulation protein SpoVR |
RA | 1,040,043 | A→C | 5.2% | E431D (GAA→GAC) | EO946_RS05365 → | AAA family ATPase |
RA | 1,225,192 | C→A | 9.5% | intergenic (‑71/‑48) | EO946_RS06345 ← / → EO946_RS06350 | ATP‑dependent helicase/hypothetical protein |
RA | 1,488,182 | G→T | 6.2% | A575S (GCA→TCA) | ade → | adenine deaminase |
RA | 1,512,556 | G→T | 5.2% | intergenic (‑84/‑29) | EO946_RS07905 ← / → typA | YlaF family protein/translational GTPase TypA |
RA | 1,514,881 | G→T | 7.8% | Q62K (CAA→AAA) | EO946_RS07920 ← | YlaI family protein |
RA | 1,582,922 | C→A | 7.2% | A38E (GCA→GAA) | ylyA → | sporulation‑related RNA polymerase‑binding protein YlyA |
RA | 1,800,874 | A→C | 15.0% | S3519R (AGC→CGC) | EO946_RS09135 → | SDR family NAD(P)‑dependent oxidoreductase |
RA | 1,936,593 | G→T | 14.8% | P3553Q (CCA→CAA) | EO946_RS09735 ← | non‑ribosomal peptide synthetase |
RA | 2,034,564 | C→A | 9.5% | intergenic (‑230/‑164) | EO946_RS10175 ← / → EO946_RS10180 | UPF0715 family protein/hypothetical protein |
RA | 2,139,480 | A→C | 6.3% | A119A (GCT→GCG) | deoD ← | purine‑nucleoside phosphorylase |
RA | 2,179,645 | G→T | 5.8% | A215S (GCC→TCC) | EO946_RS11020 → | queuosine precursor transporter |
RA | 2,214,518 | T→G | 8.3% | K60T (AAA→ACA) | nth ← | endonuclease III |
RA | 2,214,528 | G→T | 6.6% | Q57K (CAA→AAA) | nth ← | endonuclease III |
RA | 2,214,555 | C→A | 8.1% | V48L (GTA→TTA) | nth ← | endonuclease III |
RA | 2,269,970 | G→T | 6.1% | S70Y (TCT→TAT) | prsW ← | glutamic‑type intramembrane protease PrsW |
RA | 2,269,984 | G→C | 7.5% | F65L (TTC→TTG) | prsW ← | glutamic‑type intramembrane protease PrsW |
RA | 2,270,057 | A→C | 10.8% | V41G (GTT→GGT) | prsW ← | glutamic‑type intramembrane protease PrsW |
RA | 2,387,066 | T→G | 12.6% | S354R (AGC→CGC) | EO946_RS12195 ← | acetyl‑CoA C‑acetyltransferase |
RA | 2,502,242 | T→G | 21.1% | T274P (ACG→CCG) | hrcA ← | heat‑inducible transcriptional repressor HrcA |
RA | 2,532,199 | G→T | 5.0% | intergenic (+412/+251) | EO946_RS13065 → / ← EO946_RS13070 | DUF1569 domain‑containing protein/nitronate monooxygenase |
RA | 2,532,210 | A→T | 5.1% | intergenic (+423/+240) | EO946_RS13065 → / ← EO946_RS13070 | DUF1569 domain‑containing protein/nitronate monooxygenase |
RA | 2,910,843 | G→C | 6.4% | intergenic (‑43/‑147) | EO946_RS14985 ← / → EO946_RS14990 | MFS transporter/LMBR1 domain‑containing protein |
RA | 2,962,633 | G→T | 5.8% | V313L (GTA→TTA) | EO946_RS15265 → | glycosyltransferase family 4 protein |
RA | 2,979,248 | T→C | 31.2% | noncoding (1553/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
RA | 2,991,262 | G→T | 16.9% | intergenic (+63/‑144) | gbsR → / → bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
RA | 3,139,479 | C→G | 7.0% | S40C (TCT→TGT) | pucL → | urate oxidase |
RA | 3,247,065 | C→T | 100% | intergenic (‑11/+110) | bdbD ← / ← cadA | disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase |
RA | 3,268,725 | C→A | 9.4% | intergenic (+73/+137) | catR → / ← EO946_RS16920 | catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase |
RA | 3,274,191 | G→T | 6.9% | intergenic (‑528/+58) | EO946_RS16945 ← / ← EO946_RS16950 | NisI/SpaI family lantibiotic immunity lipoprotein/gallidermin/nisin family lantibiotic |
RA | 3,280,306 | G→T | 5.5% | N138K (AAC→AAA) | EO946_RS16965 ← | lantibiotic dehydratase |
RA | 3,285,567 | C→T | 36.9% | L278L (CTG→CTA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,575 | T→G | 37.2% | N276H (AAT→CAT) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,580 | A→G | 39.3% | I274T (ATA→ACA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,647 | C→A | 41.8% | V252L (GTA→TTA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,713 | G→A | 29.4% | L230L (CTA→TTA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,446,970 | G→T | 21.0% | Q206K (CAA→AAA) | degU ← | two‑component system response regulator DegU |
RA | 3,446,987 | T→A | 7.0% | H200L (CAT→CTT) | degU ← | two‑component system response regulator DegU |
RA | 3,446,995 | C→A | 8.1% | V197V (GTG→GTT) | degU ← | two‑component system response regulator DegU |
RA | 3,447,038 | T→G | 10.7% | N183T (AAC→ACC) | degU ← | two‑component system response regulator DegU |
RA | 3,496,440 | G→T | 6.6% | Q330K (CAA→AAA) | EO946_RS17995 ← | glucosaminidase domain‑containing protein |
RA | 3,559,345 | T→G | 7.2% | N205H (AAC→CAC) | EO946_RS18295 ← | SpaA isopeptide‑forming pilin‑related protein |
RA | 3,562,015 | A→C | 12.5% | intergenic (‑661/‑137) | EO946_RS18300 ← / → EO946_RS21105 | response regulator/HAMP domain‑containing histidine kinase |
RA | 3,584,316 | G→C | 7.9% | E333Q (GAA→CAA) | EO946_RS18420 → | N‑acetylneuraminate synthase family protein |
RA | 3,746,962 | C→A | 5.5% | D36Y (GAT→TAT) | EO946_RS19275 ← | galactokinase |
RA | 3,769,576 | T→G | 7.6% | I281S (ATT→AGT) | sacX → | SacY negative regulator SacX |
RA | 3,851,230 | G→T | 5.8% | Q49K (CAG→AAG) | EO946_RS19820 ← | hypothetical protein |
RA | 3,851,254 | G→C | 5.3% | L41V (CTT→GTT) | EO946_RS19820 ← | hypothetical protein |
RA | 3,851,274 | A→C | 18.4% | F34C (TTT→TGT) | EO946_RS19820 ← | hypothetical protein |
RA | 3,996,851 | A→T | 10.6% | intergenic (‑157/+49) | purA ← / ← EO946_RS20530 | adenylosuccinate synthase/VOC family protein |
RA | 3,996,857 | C→A | 6.6% | intergenic (‑163/+43) | purA ← / ← EO946_RS20530 | adenylosuccinate synthase/VOC family protein |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP034943 | = 91969 | NA (NA) | 28 (0.090) | 10/250 | NT | NA | intergenic (+131/‑39) | EO946_RS00485/EO946_RS00490 | 16S ribosomal RNA/23S ribosomal RNA |
? | NZ_CP034943 | 91973 = | NA (NA) | intergenic (+135/‑35) | EO946_RS00485/EO946_RS00490 | 16S ribosomal RNA/23S ribosomal RNA | |||||
* | ? | NZ_CP034943 | 917510 = | 509 (1.620) | 1216 (3.900) +G |
4/250 | NT | 73.0% | intergenic (+294/‑1) | EO946_RS04665/EO946_RS04670 | nucleotidyltransferase‑like protein/16S ribosomal RNA |
? | NZ_CP034943 | 2982587 = | 398 (1.270) | intergenic (‑68/‑606) | EO946_RS15435/thiT | 16S ribosomal RNA/energy‑coupled thiamine transporter ThiT |