breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 44,865 T→A 21.0% intergenic (‑46/‑455) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
RA 44,870 C→T 21.0% intergenic (‑51/‑450) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
RA 44,871 G→C 20.9% intergenic (‑52/‑449) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
RA 44,881 G→A 76.7% intergenic (‑62/‑439) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
RA 132,099 T→C 62.4% V531A (GTA→GCA)  fusA → elongation factor G
RA 132,185 G→A 24.2% D560N (GAC→AAC)  fusA → elongation factor G
RA 135,117 G→T 6.5% intergenic (+164/‑74) EO946_RS00715 → / → rpsJ alpha/beta hydrolase/30S ribosomal protein S10
RA 209,812 G→T 5.3% A234S (GCA→TCA)  EO946_RS01190 → alpha/beta hydrolase
RA 240,211 G→T 11.6% A107A (GCC→GCA EO946_RS01360 ← hypothetical protein
RA 316,262 C→A 8.2% Q28K (CAA→AAA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA
RA 399,035 C→A 8.2% V949V (GTC→GTA srfAC → surfactin non‑ribosomal peptide synthetase SrfAC
RA 607,359 A→C 13.5% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
JC 607,424 27 bp→108 bp 47.1% coding (267‑293/1125 nt) EO946_RS03185 → collagen‑like protein
RA 607,529 G→C 6.4% A124A (GCG→GCC EO946_RS03185 → collagen‑like protein
RA 607,562 G→A 6.3% G135G (GGG→GGA EO946_RS03185 → collagen‑like protein
RA 607,565 C→G 9.8% A136A (GCC→GCG EO946_RS03185 → collagen‑like protein
RA 607,638 G→C 9.5% A161P (GCC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,700 A→C 16.7% E181D (GAA→GAC EO946_RS03185 → collagen‑like protein
JC 607,746 Δ45 bp 8.4% coding (589‑633/1125 nt) EO946_RS03185 → collagen‑like protein
RA 607,838 A→G 8.8% T227T (ACA→ACG EO946_RS03185 → collagen‑like protein
RA 634,086 G→T 6.2% S562* (TCA→TAA)  EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein
RA 814,356 T→G 8.8% H340Q (CAT→CAG nosA → nitric oxide synthase
RA 886,747 A→C 10.5% P47P (CCT→CCG EO946_RS04490 ← MFS transporter
RA 924,135 A→T 5.4% intergenic (+10/+41) EO946_RS04760 → / ← spo0M tRNA‑Leu/sporulation control protein Spo0M
RA 986,801 G→T 10.3% D372Y (GAT→TAT)  spoVR → stage V sporulation protein SpoVR
RA 1,040,043 A→C 5.2% E431D (GAA→GAC EO946_RS05365 → AAA family ATPase
RA 1,225,192 C→A 9.5% intergenic (‑71/‑48) EO946_RS06345 ← / → EO946_RS06350 ATP‑dependent helicase/hypothetical protein
RA 1,488,182 G→T 6.2% A575S (GCA→TCA)  ade → adenine deaminase
RA 1,512,556 G→T 5.2% intergenic (‑84/‑29) EO946_RS07905 ← / → typA YlaF family protein/translational GTPase TypA
RA 1,514,881 G→T 7.8% Q62K (CAA→AAA)  EO946_RS07920 ← YlaI family protein
RA 1,582,922 C→A 7.2% A38E (GCA→GAA)  ylyA → sporulation‑related RNA polymerase‑binding protein YlyA
RA 1,800,874 A→C 15.0% S3519R (AGC→CGC)  EO946_RS09135 → SDR family NAD(P)‑dependent oxidoreductase
RA 1,936,593 G→T 14.8% P3553Q (CCA→CAA)  EO946_RS09735 ← non‑ribosomal peptide synthetase
RA 2,034,564 C→A 9.5% intergenic (‑230/‑164) EO946_RS10175 ← / → EO946_RS10180 UPF0715 family protein/hypothetical protein
RA 2,139,480 A→C 6.3% A119A (GCT→GCG deoD ← purine‑nucleoside phosphorylase
RA 2,179,645 G→T 5.8% A215S (GCC→TCC)  EO946_RS11020 → queuosine precursor transporter
RA 2,214,518 T→G 8.3% K60T (AAA→ACA)  nth ← endonuclease III
RA 2,214,528 G→T 6.6% Q57K (CAA→AAA)  nth ← endonuclease III
RA 2,214,555 C→A 8.1% V48L (GTA→TTA)  nth ← endonuclease III
RA 2,269,970 G→T 6.1% S70Y (TCT→TAT)  prsW ← glutamic‑type intramembrane protease PrsW
RA 2,269,984 G→C 7.5% F65L (TTC→TTG prsW ← glutamic‑type intramembrane protease PrsW
RA 2,270,057 A→C 10.8% V41G (GTT→GGT)  prsW ← glutamic‑type intramembrane protease PrsW
RA 2,387,066 T→G 12.6% S354R (AGC→CGC)  EO946_RS12195 ← acetyl‑CoA C‑acetyltransferase
RA 2,502,242 T→G 21.1% T274P (ACG→CCG)  hrcA ← heat‑inducible transcriptional repressor HrcA
RA 2,532,199 G→T 5.0% intergenic (+412/+251) EO946_RS13065 → / ← EO946_RS13070 DUF1569 domain‑containing protein/nitronate monooxygenase
RA 2,532,210 A→T 5.1% intergenic (+423/+240) EO946_RS13065 → / ← EO946_RS13070 DUF1569 domain‑containing protein/nitronate monooxygenase
RA 2,910,843 G→C 6.4% intergenic (‑43/‑147) EO946_RS14985 ← / → EO946_RS14990 MFS transporter/LMBR1 domain‑containing protein
RA 2,962,633 G→T 5.8% V313L (GTA→TTA)  EO946_RS15265 → glycosyltransferase family 4 protein
RA 2,979,248 T→C 31.2% noncoding (1553/2928 nt) EO946_RS15430 ← 23S ribosomal RNA
RA 2,991,262 G→T 16.9% intergenic (+63/‑144) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA
RA 3,139,479 C→G 7.0% S40C (TCT→TGT)  pucL → urate oxidase
RA 3,247,065 C→T 100% intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
RA 3,268,725 C→A 9.4% intergenic (+73/+137) catR → / ← EO946_RS16920 catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase
RA 3,274,191 G→T 6.9% intergenic (‑528/+58) EO946_RS16945 ← / ← EO946_RS16950 NisI/SpaI family lantibiotic immunity lipoprotein/gallidermin/nisin family lantibiotic
RA 3,280,306 G→T 5.5% N138K (AAC→AAA EO946_RS16965 ← lantibiotic dehydratase
RA 3,285,567 C→T 36.9% L278L (CTG→CTA EO946_RS16995 ← IS3 family transposase
RA 3,285,575 T→G 37.2% N276H (AAT→CAT)  EO946_RS16995 ← IS3 family transposase
RA 3,285,580 A→G 39.3% I274T (ATA→ACA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,647 C→A 41.8% V252L (GTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,713 G→A 29.4% L230L (CTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,446,970 G→T 21.0% Q206K (CAA→AAA)  degU ← two‑component system response regulator DegU
RA 3,446,987 T→A 7.0% H200L (CAT→CTT)  degU ← two‑component system response regulator DegU
RA 3,446,995 C→A 8.1% V197V (GTG→GTT degU ← two‑component system response regulator DegU
RA 3,447,038 T→G 10.7% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU
RA 3,496,440 G→T 6.6% Q330K (CAA→AAA)  EO946_RS17995 ← glucosaminidase domain‑containing protein
RA 3,559,345 T→G 7.2% N205H (AAC→CAC)  EO946_RS18295 ← SpaA isopeptide‑forming pilin‑related protein
RA 3,562,015 A→C 12.5% intergenic (‑661/‑137) EO946_RS18300 ← / → EO946_RS21105 response regulator/HAMP domain‑containing histidine kinase
RA 3,584,316 G→C 7.9% E333Q (GAA→CAA)  EO946_RS18420 → N‑acetylneuraminate synthase family protein
RA 3,746,962 C→A 5.5% D36Y (GAT→TAT)  EO946_RS19275 ← galactokinase
RA 3,769,576 T→G 7.6% I281S (ATT→AGT)  sacX → SacY negative regulator SacX
RA 3,851,230 G→T 5.8% Q49K (CAG→AAG)  EO946_RS19820 ← hypothetical protein
RA 3,851,254 G→C 5.3% L41V (CTT→GTT)  EO946_RS19820 ← hypothetical protein
RA 3,851,274 A→C 18.4% F34C (TTT→TGT)  EO946_RS19820 ← hypothetical protein
RA 3,996,851 A→T 10.6% intergenic (‑157/+49) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein
RA 3,996,857 C→A 6.6% intergenic (‑163/+43) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 91969NA (NA)28 (0.090) 10/250 NT NA intergenic (+131/‑39) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
?NZ_CP034943 91973 = NA (NA)intergenic (+135/‑35) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
* ? NZ_CP034943 917510 =509 (1.620)1216 (3.900)
+G
4/250 NT 73.0% intergenic (+294/‑1) EO946_RS04665/EO946_RS04670 nucleotidyltransferase‑like protein/16S ribosomal RNA
?NZ_CP034943 2982587 = 398 (1.270)intergenic (‑68/‑606) EO946_RS15435/thiT 16S ribosomal RNA/energy‑coupled thiamine transporter ThiT