breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 9,720 G→A S24N (AGC→AAC)  RPPX_00065 → hypothetical protein
RA 25,728 G→A intergenic (+153/‑418) RPPX_00140 → / → RPPX_00145 MarR family transcriptional regulator/(2Fe‑2S)‑binding protein
RA 25,739 A→T intergenic (+164/‑407) RPPX_00140 → / → RPPX_00145 MarR family transcriptional regulator/(2Fe‑2S)‑binding protein
RA 25,890 G→A intergenic (+315/‑256) RPPX_00140 → / → RPPX_00145 MarR family transcriptional regulator/(2Fe‑2S)‑binding protein
RA 139,240 G→T intergenic (+242/+57) RPPX_00595 → / ← RPPX_00600 3‑methylcrotonyl‑CoA carboxylase/Cro/Cl family transcriptional regulator
RA 382,164 T→C intergenic (+300/+229) RPPX_01535 → / ← RPPX_01540 guanine permease/5‑hydroxyisourate hydrolase
RA 382,181 T→C intergenic (+317/+212) RPPX_01535 → / ← RPPX_01540 guanine permease/5‑hydroxyisourate hydrolase
RA 382,187 C→T intergenic (+323/+206) RPPX_01535 → / ← RPPX_01540 guanine permease/5‑hydroxyisourate hydrolase
RA 382,193 T→C intergenic (+329/+200) RPPX_01535 → / ← RPPX_01540 guanine permease/5‑hydroxyisourate hydrolase
RA 418,393 C→T C10Y (TGC→TAC)  RPPX_01715 ← hypothetical protein
RA 418,408 C→G C5S (TGC→TCC)  RPPX_01715 ← hypothetical protein
RA 418,441 Δ1 bp intergenic (‑20/+631) RPPX_01715 ← / ← RPPX_01720 hypothetical protein/lipoprotein
RA 418,449 Δ1 bp intergenic (‑28/+623) RPPX_01715 ← / ← RPPX_01720 hypothetical protein/lipoprotein
RA 542,045 T→C intergenic (‑306/+223) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator
RA 542,069 T→C intergenic (‑330/+199) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator
RA 542,073 T→C intergenic (‑334/+195) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator
RA 542,098 A→T intergenic (‑359/+170) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator
RA 542,104 C→G intergenic (‑365/+164) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator
RA 542,115 2 bp→TG intergenic (‑376/+152) argD ← / ← RPPX_02300 acetylornithine aminotransferase/AraC family transcriptional regulator
RA 554,461 G→A P432P (CCG→CCA RPPX_02350 → hypothetical protein
RA 654,275 G→A L73L (CTG→CTA RPPX_02810 → 23S rRNA methyltransferase
RA 659,171 A→G V185A (GTC→GCC)  RPPX_02840 ← ribonuclease D
RA 753,050 A→G intergenic (+50/‑204) RPPX_03275 → / → RPPX_03280 porin/NAD‑dependent dehydratase
RA 768,193 A→G G462G (GGT→GGC RPPX_03350 ← acetolactate synthase
RA 775,414 C→T intergenic (‑176/‑282) RPPX_03375 ← / → RPPX_03380 16S ribosomal RNA/membrane protein
RA 775,456 G→A intergenic (‑218/‑240) RPPX_03375 ← / → RPPX_03380 16S ribosomal RNA/membrane protein
RA 775,486 G→A intergenic (‑248/‑210) RPPX_03375 ← / → RPPX_03380 16S ribosomal RNA/membrane protein
RA 775,488 G→C intergenic (‑250/‑208) RPPX_03375 ← / → RPPX_03380 16S ribosomal RNA/membrane protein
RA 1,050,398 G→A A205T (GCC→ACC)  RPPX_04625 → fumarate hydratase
RA 1,154,311 T→C T337T (ACT→ACC RPPX_05115 → hypothetical protein
RA 1,154,773 2 bp→GG coding (1473‑1474/2580 nt) RPPX_05115 → hypothetical protein
RA 1,155,980 A→T T23S (ACC→TCT)  RPPX_05120 → hypothetical protein
RA 1,155,982 C→T T23S (ACC→TCT RPPX_05120 → hypothetical protein
RA 1,158,307 G→A intergenic (+1830/‑13453) RPPX_05120 → / → RPPX_05130 hypothetical protein/channel protein TolC
RA 1,158,399 G→C intergenic (+1922/‑13361) RPPX_05120 → / → RPPX_05130 hypothetical protein/channel protein TolC
RA 1,158,403 A→G intergenic (+1926/‑13357) RPPX_05120 → / → RPPX_05130 hypothetical protein/channel protein TolC
RA 1,159,719 T→A intergenic (+3242/‑12041) RPPX_05120 → / → RPPX_05130 hypothetical protein/channel protein TolC
RA 1,160,779 C→T intergenic (+4302/‑10981) RPPX_05120 → / → RPPX_05130 hypothetical protein/channel protein TolC
RA 1,160,785 T→C intergenic (+4308/‑10975) RPPX_05120 → / → RPPX_05130 hypothetical protein/channel protein TolC
RA 1,174,412 A→G D407G (GAT→GGT)  RPPX_05135 → ATP‑binding protein
RA 1,215,711 G→C intergenic (‑9/+17) RPPX_05325 ← / ← RPPX_05330 hypothetical protein/type V secretory pathway, adhesin AidA
RA 1,247,078 G→C intergenic (‑212/‑599) RPPX_05495 ← / → RPPX_05500 glutamyl‑tRNA reductase/hypothetical protein
RA 1,247,115 2 bp→T intergenic (‑249/‑561) RPPX_05495 ← / → RPPX_05500 glutamyl‑tRNA reductase/hypothetical protein
MC JC 1,303,424 Δ2,602 bp ISS12‑mediated RPPX_05775RPPX_05780 RPPX_05775, RPPX_05780
MC JC 1,343,208 Δ2,581 bp RPPX_05930RPPX_05935 RPPX_05930, RPPX_05935
RA 1,346,042 2 bp→GT intergenic (+365/+561) RPPX_05935 → / ← RPPX_05940 ATPase AAA/DNA‑binding protein
RA 1,358,374 G→T intergenic (+235/+2455) RPPX_05970 → / ← RPPX_05980 hypothetical protein/tRNA‑Thr
RA 1,358,392 C→A intergenic (+253/+2437) RPPX_05970 → / ← RPPX_05980 hypothetical protein/tRNA‑Thr
RA 1,358,394 T→A intergenic (+255/+2435) RPPX_05970 → / ← RPPX_05980 hypothetical protein/tRNA‑Thr
RA 1,358,424 2 bp→TC intergenic (+285/+2404) RPPX_05970 → / ← RPPX_05980 hypothetical protein/tRNA‑Thr
RA 1,358,523 C→A intergenic (+384/+2306) RPPX_05970 → / ← RPPX_05980 hypothetical protein/tRNA‑Thr
RA 1,449,631 T→C intergenic (+144/‑45) RPPX_06395 → / → RPPX_06400 chemotaxis protein/thiol:disulfide interchange protein
RA 1,490,016 G→A intergenic (+31/‑317) RPPX_06570 → / → RPPX_06575 1‑deoxy‑D‑xylulose‑5‑phosphate synthase/TonB‑dependent receptor
RA 1,490,020 G→C intergenic (+35/‑313) RPPX_06570 → / → RPPX_06575 1‑deoxy‑D‑xylulose‑5‑phosphate synthase/TonB‑dependent receptor
RA 1,533,045 G→A S453S (TCG→TCA RPPX_06795 → GntR family transcriptional regulator
RA 1,554,345 A→T V354V (GTT→GTA tuf ← elongation factor Tu
RA 1,554,630 A→G C259C (TGT→TGC tuf ← elongation factor Tu
RA 1,554,729 C→A G226G (GGG→GGT tuf ← elongation factor Tu
RA 1,554,744 T→C V221V (GTA→GTG tuf ← elongation factor Tu
RA 1,554,990 A→G D139D (GAT→GAC tuf ← elongation factor Tu
RA 1,555,277 T→C I44V (ATC→GTC)  tuf ← elongation factor Tu
RA 1,555,302 A→G V35V (GTT→GTC tuf ← elongation factor Tu
RA 1,561,107 T→C D622G (GAC→GGC)  RPPX_06985 ← DNA‑directed RNA polymerase subunit beta'
RA 1,796,148 C→T Y243Y (TAC→TAT RPPX_08005 → integrase
RA 1,844,787 G→A R199Q (CGG→CAG)  RPPX_08230 → monooxygenase
RA 1,896,148 T→C intergenic (‑3926/‑4671) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
RA 1,896,150 C→G intergenic (‑3928/‑4669) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
RA 1,897,249 G→A intergenic (‑5027/‑3570) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
RA 1,897,594 G→C intergenic (‑5372/‑3225) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
RA 1,897,654 T→C intergenic (‑5432/‑3165) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
RA 1,897,666 G→A intergenic (‑5444/‑3153) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
RA 1,897,669 A→G intergenic (‑5447/‑3150) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
RA 1,897,927 A→G intergenic (‑5705/‑2892) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
RA 1,897,951 G→T intergenic (‑5729/‑2868) RPPX_08470 ← / → RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
RA 1,915,165 T→C intergenic (+54/+14) RPPX_08535 → / ← RPPX_08540 ferrioxamine B receptor/5S ribosomal RNA
RA 1,915,170 Δ1 bp intergenic (+59/+9) RPPX_08535 → / ← RPPX_08540 ferrioxamine B receptor/5S ribosomal RNA
RA 1,915,172 3 bp→GTT intergenic (+61/+5) RPPX_08535 → / ← RPPX_08540 ferrioxamine B receptor/5S ribosomal RNA
RA 1,915,245 A→G noncoding (50/116 nt) RPPX_08540 ← 5S ribosomal RNA
RA 1,920,735 T→C intergenic (‑364/+191) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,738 T→C intergenic (‑367/+188) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,747 T→C intergenic (‑376/+179) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,749 A→C intergenic (‑378/+177) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,754 1 bp→TTT intergenic (‑383/+172) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,759 C→T intergenic (‑388/+167) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,761 Δ1 bp intergenic (‑390/+165) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,763 A→C intergenic (‑392/+163) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,765 2 bp→AC intergenic (‑394/+160) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,768 Δ1 bp intergenic (‑397/+158) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,794 G→T intergenic (‑423/+132) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,920,803 T→A intergenic (‑432/+123) RPPX_08560 ← / ← RPPX_08565 16S ribosomal RNA/CoA‑transferase
RA 1,984,399 T→C intergenic (+229/+55) RPPX_08865 → / ← RPPX_08870 hypothetical protein/SulP family inorganic anion transporter
RA 1,994,226 G→A intergenic (+26/+84) RPPX_08905 → / ← RPPX_08910 HAD family hydrolase/aminopeptidase
RA 2,034,815 G→A A82A (GCC→GCT RPPX_09120 ← beta‑lactamase
RA 2,122,818 C→T R165C (CGT→TGT)  RPPX_09510 → ATP F0F1 synthase subunit alpha
RA 2,331,550 C→T Q88Q (CAG→CAA RPPX_10445 ← membrane protein
RA 2,336,379 (C)10→9 intergenic (‑343/+12) RPPX_10460 ← / ← RPPX_10465 EVE domain protein/5‑formyltetrahydrofolate cyclo‑ligase
RA 2,337,138 A→G intergenic (‑139/+182) RPPX_10465 ← / ← RPPX_10470 5‑formyltetrahydrofolate cyclo‑ligase/cell division protein ZapA
MC JC 2,509,653 Δ2,602 bp RPPX_11210[RPPX_11220] RPPX_11210, RPPX_11215, [RPPX_11220]
RA 2,738,465 T→C A226A (GCT→GCC RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,471 C→T T228T (ACC→ACT RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,498 G→A A237A (GCG→GCA RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,501 T→C G238G (GGT→GGC RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,507 G→A E240E (GAG→GAA RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,513 G→A K242K (AAG→AAA RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,522 T→C S245S (AGT→AGC RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,525 T→C A246A (GCT→GCC RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,529 G→T A248S (GCC→TCT)  RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,531 C→T A248S (GCC→TCT RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,537 C→T A250A (GCC→GCT RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,546 C→T G253G (GGC→GGT RPPX_12165 → benzene 1,2‑dioxygenase
RA 2,738,549 C→T G254G (GGC→GGT RPPX_12165 → benzene 1,2‑dioxygenase
MC JC 2,756,352 Δ2,603 bp [RPPX_12250]RPPX_12260 [RPPX_12250], RPPX_12255, RPPX_12260
MC JC 2,767,372 Δ2,604 bp RPPX_12300RPPX_12305 RPPX_12300, RPPX_12305
JC 2,783,938 (CTTGCGTT)7→8 intergenic (+386/‑55) RPPX_12345 → / → RPPX_12350 hypothetical protein/hypothetical protein
RA 2,825,986 G→A A69V (GCC→GTC)  RPPX_12545 ← hypothetical protein
RA 2,867,069 G→A intergenic (‑156/+60) RPPX_12750 ← / ← RPPX_12755 lipoyl synthase/DNA polymerase III subunit delta
RA 2,910,161 T→C intergenic (+65/‑594) gudD → / → RPPX_12965 glucarate dehydratase/GABA permease
RA 2,910,232 T→C intergenic (+136/‑523) gudD → / → RPPX_12965 glucarate dehydratase/GABA permease
RA 2,910,258 A→G intergenic (+162/‑497) gudD → / → RPPX_12965 glucarate dehydratase/GABA permease
MC JC 2,913,100 Δ2,603 bp RPPX_12970[RPPX_12980] RPPX_12970, RPPX_12975, [RPPX_12980]
RA 2,982,223 T→G intergenic (‑302/+644) RPPX_13295 ← / ← RPPX_13300 integrase/integrase
RA 2,982,248 T→G intergenic (‑327/+619) RPPX_13295 ← / ← RPPX_13300 integrase/integrase
RA 3,079,418 C→T intergenic (+46/+85) RPPX_13695 → / ← RPPX_13700 membrane protein/LysR family transcriptional regulator
RA 3,148,563 G→A intergenic (+90/+122) RPPX_14035 → / ← RPPX_14040 membrane protein/bifunctional glyoxylate/hydroxypyruvate reductase B
JC 3,148,596 +96 bp intergenic (+123/+89) RPPX_14035 → / ← RPPX_14040 membrane protein/bifunctional glyoxylate/hydroxypyruvate reductase B
RA 3,161,709 T→C intergenic (+115/+136) RPPX_14090 → / ← RPPX_14095 RND transporter/short‑chain dehydrogenase
RA 3,288,855 G→A G198R (GGG→AGG)  RPPX_14650 → TetR family transcriptional regulator
RA 3,297,191 +G coding (158/921 nt) RPPX_14685 ← AraC family transcriptional regulator
RA 3,297,192 T→G T53P (ACC→CCC)  RPPX_14685 ← AraC family transcriptional regulator
RA 3,400,410 A→G T381A (ACC→GCC)  RPPX_15160 → chemotaxis protein
RA 3,406,966 +T intergenic (+325/‑1049) RPPX_15185 → / → RPPX_15195 chemotaxis protein CheY/peptide chain release factor 2
MC JC 3,486,773 Δ2,604 bp RPPX_15555RPPX_15560 RPPX_15555, RPPX_15560
RA 3,492,467 (T)5→4 coding (124/2574 nt) RPPX_15570 ← DNA mismatch repair protein MutS
MC JC 3,503,916 Δ2,603 bp RPPX_15645RPPX_15650 RPPX_15645, RPPX_15650
RA 3,514,540 (G)6→5 coding (316/2754 nt) RPPX_15700 ← chemotaxis protein CheY
RA 3,530,581 C→T G54S (GGC→AGC)  RPPX_15770 ← phosphoribosylaminoimidazole synthetase
RA 3,535,334 A→G D113G (GAC→GGC)  RPPX_15795 → hydrolase Nlp/P60
RA 3,566,025 T→G intergenic (‑154/+160) RPPX_15935 ← / ← RPPX_15940 molecular chaperone/recombinase RdgC
RA 3,566,031 C→G intergenic (‑160/+154) RPPX_15935 ← / ← RPPX_15940 molecular chaperone/recombinase RdgC
RA 3,566,083 C→T intergenic (‑212/+102) RPPX_15935 ← / ← RPPX_15940 molecular chaperone/recombinase RdgC
RA 3,585,070 C→G intergenic (+210/+13) RPPX_16020 → / ← RPPX_16025 hypothetical protein/diguanylate phosphodiesterase
RA 3,614,972 A→G T438A (ACC→GCC)  RPPX_16160 → pilus assembly protein PapC
RA 3,673,509 C→T intergenic (+108/‑429) RPPX_16400 → / → RPPX_16405 glucose‑1‑phosphate thymidylyltransferase/beta‑ketoacyl synthase
RA 3,721,374 A→G intergenic (+316/+51) RPPX_16570 → / ← RPPX_16575 glutathione S‑transferase/glutaredoxin
RA 3,756,668 C→G intergenic (+550/+90) RPPX_16775 → / ← RPPX_16780 hypothetical protein/phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
RA 3,756,907 G→C L400V (CTC→GTC)  RPPX_16780 ← phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
RA 3,756,917 G→A T396T (ACC→ACT RPPX_16780 ← phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
RA 3,756,925 G→C Q394E (CAG→GAG)  RPPX_16780 ← phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
RA 3,756,930 A→T L392H (CTT→CAT)  RPPX_16780 ← phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
RA 3,756,936 T→C D390G (GAT→GGT)  RPPX_16780 ← phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
RA 3,765,881 C→T D109D (GAC→GAT RPPX_16815 → peptide methionine sulfoxide reductase
RA 3,793,987 2 bp→CC coding (293‑294/3894 nt) RPPX_16900 → YD repeat‑/RHS repeat‑containing protein
RA 3,837,678 C→T intergenic (+229/‑322) RPPX_17095 → / → RPPX_17100 proteophosphoglycan precursor/cupin
RA 3,837,781 C→T intergenic (+332/‑219) RPPX_17095 → / → RPPX_17100 proteophosphoglycan precursor/cupin
RA 3,837,790 T→C intergenic (+341/‑210) RPPX_17095 → / → RPPX_17100 proteophosphoglycan precursor/cupin
RA 3,837,799 A→G intergenic (+350/‑201) RPPX_17095 → / → RPPX_17100 proteophosphoglycan precursor/cupin
RA 3,844,127 C→T intergenic (+345/‑1) RPPX_17120 → / → RPPX_17125 glutamyl‑tRNA synthetase/tRNA‑Ala
JC 3,858,455 (GAAGCAA)5→13 intergenic (+458/+161) RPPX_17190 → / ← RPPX_17195 aspartate‑semialdehyde dehydrogenase/hypothetical protein
JC 3,898,294 (TGGCCGTCGGGTTGCCGAGGACGGTGCAGACCGACTCAA)1→2 coding (197/246 nt) RPPX_17380 → hypothetical protein
RA 3,961,904 C→T T416T (ACC→ACT RPPX_17635 → damage‑inducible protein CinA
RA 3,971,951 G→T S9S (TCC→TCA RPPX_17675 ← hemolysin D
RA 4,030,927 G→A intergenic (+69/‑715) RPPX_17905 → / → RPPX_17915 23S rRNA methyltransferase/sulfurtransferase
RA 4,031,017 Δ1 bp intergenic (+159/‑625) RPPX_17905 → / → RPPX_17915 23S rRNA methyltransferase/sulfurtransferase
RA 4,132,231 A→C intergenic (+147/+82) RPPX_18375 → / ← RPPX_18380 membrane protein/phosphonoacetaldehyde hydrolase
RA 4,147,169 (CTTCTTG)5→6 intergenic (+421/‑600) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR
RA 4,147,204 A→G intergenic (+456/‑565) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR
RA 4,147,209 A→G intergenic (+461/‑560) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR
RA 4,147,332 A→T intergenic (+584/‑437) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR
RA 4,147,342 T→C intergenic (+594/‑427) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR
RA 4,147,416 C→G intergenic (+668/‑353) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR
RA 4,147,431 A→G intergenic (+683/‑338) RPPX_18425 → / → RPPX_18430 enoyl‑CoA hydratase/conjugal transfer protein TraR
RA 4,165,100 C→T intergenic (‑294/‑181) RPPX_18525 ← / → RPPX_18530 transporter/16S ribosomal RNA
RA 4,165,105 G→A intergenic (‑299/‑176) RPPX_18525 ← / → RPPX_18530 transporter/16S ribosomal RNA
RA 4,165,107 T→C intergenic (‑301/‑174) RPPX_18525 ← / → RPPX_18530 transporter/16S ribosomal RNA
RA 4,165,118 Δ1 bp intergenic (‑312/‑163) RPPX_18525 ← / → RPPX_18530 transporter/16S ribosomal RNA
RA 4,170,224 T→C noncoding (2917/2917 nt) RPPX_18545 → 23S ribosomal RNA
RA 4,242,144 G→A G443D (GGC→GAC)  RPPX_18895 → aconitate hydratase
RA 4,258,919 C→G intergenic (+132/+99) prpE → / ← RPPX_18965 propionyl‑CoA synthetase/ATPase
RA 4,286,522 C→T intergenic (+378/+266) RPPX_19070 → / ← RPPX_19075 LacI family transcriptional regulator/hypothetical protein
RA 4,363,550 T→C intergenic (+206/‑109) RPPX_19410 → / → RPPX_19415 alkyl hydroperoxide reductase/hypothetical protein
RA 4,384,773 A→G G174G (GGA→GGG RPPX_19530 → nucleoside hydrolase
MC JC 4,425,765 Δ2,602 bp RPPX_19815RPPX_19820 RPPX_19815, RPPX_19820
RA 4,537,108 A→G Y105Y (TAT→TAC RPPX_20270 ← hypothetical protein
RA 4,537,135 C→G L96L (CTG→CTC RPPX_20270 ← hypothetical protein
RA 4,540,058 T→C A592A (GCA→GCG RPPX_20280 ← hypothetical protein
RA 4,540,457 A→G R459R (CGT→CGC RPPX_20280 ← hypothetical protein
RA 4,540,460 C→T R458R (CGG→CGA RPPX_20280 ← hypothetical protein
RA 4,541,504 C→T L110L (CTG→CTA RPPX_20280 ← hypothetical protein
RA 4,541,636 C→T P66P (CCG→CCA RPPX_20280 ← hypothetical protein
RA 4,541,648 A→G R62R (CGT→CGC RPPX_20280 ← hypothetical protein
RA 4,543,770 A→G A451A (GCT→GCC RPPX_20290 ← hypothetical protein
RA 4,543,776 C→G K449N (AAG→AAC RPPX_20290 ← hypothetical protein
RA 4,543,787 G→A L446L (CTA→TTA)  RPPX_20290 ← hypothetical protein
RA 4,543,809 C→T A438A (GCG→GCA RPPX_20290 ← hypothetical protein
RA 4,543,833 C→G S430S (TCG→TCC RPPX_20290 ← hypothetical protein
RA 4,544,682 T→G S147S (TCA→TCC RPPX_20290 ← hypothetical protein
RA 4,544,703 G→A P140P (CCC→CCT RPPX_20290 ← hypothetical protein
RA 4,544,907 T→C T72T (ACA→ACG RPPX_20290 ← hypothetical protein
RA 4,631,683 G→A intergenic (+245/‑585) emrB → / → RPPX_20645 multidrug resistance protein B/oxidoreductase
RA 4,631,689 Δ1 bp intergenic (+251/‑579) emrB → / → RPPX_20645 multidrug resistance protein B/oxidoreductase
RA 4,632,092 C→G intergenic (+654/‑176) emrB → / → RPPX_20645 multidrug resistance protein B/oxidoreductase
RA 4,632,096 G→C intergenic (+658/‑172) emrB → / → RPPX_20645 multidrug resistance protein B/oxidoreductase
RA 4,693,000 (C)5→6 coding (1974/5865 nt) RPPX_20910 → hypothetical protein
RA 4,759,297 T→C A167A (GCA→GCG RPPX_21175 ← diguanylate phosphodiesterase
MC JC 4,905,871 Δ2,603 bp RPPX_21790RPPX_21795 RPPX_21790, RPPX_21795
RA 4,957,158 C→G V197V (GTC→GTG RPPX_21945 → ATPase AAA
RA 4,957,179 C→T D204D (GAC→GAT RPPX_21945 → ATPase AAA
RA 4,957,191 G→A A208A (GCG→GCA RPPX_21945 → ATPase AAA
RA 4,957,218 C→T D217D (GAC→GAT RPPX_21945 → ATPase AAA
RA 4,957,233 2 bp→GT coding (666‑667/756 nt) RPPX_21945 → ATPase AAA
RA 5,007,281 A→T Y68F (TAC→TTC)  RPPX_22185 → phenylacetate‑‑CoA ligase
RA 5,007,303 T→C D75D (GAT→GAC RPPX_22185 → phenylacetate‑‑CoA ligase
RA 5,007,323 T→A F82Y (TTC→TAC)  RPPX_22185 → phenylacetate‑‑CoA ligase
RA 5,007,375 C→T S99S (AGC→AGT RPPX_22185 → phenylacetate‑‑CoA ligase
RA 5,081,736 T→C D46G (GAC→GGC)  RPPX_22555 ← TenA family transcriptional regulator
RA 5,105,351 T→C P167P (CCA→CCG RPPX_22650 ← benzene 1,2‑dioxygenase
RA 5,105,354 T→C K166K (AAA→AAG RPPX_22650 ← benzene 1,2‑dioxygenase
RA 5,105,366 A→G N162N (AAT→AAC RPPX_22650 ← benzene 1,2‑dioxygenase
RA 5,105,372 A→G S160S (AGT→AGC RPPX_22650 ← benzene 1,2‑dioxygenase
RA 5,105,378 A→G F158F (TTT→TTC RPPX_22650 ← benzene 1,2‑dioxygenase
RA 5,105,383 2 bp→AA coding (468‑469/1359 nt) RPPX_22650 ← benzene 1,2‑dioxygenase
RA 5,105,402 G→A F150F (TTC→TTT RPPX_22650 ← benzene 1,2‑dioxygenase
RA 5,105,405 T→A R149R (CGA→CGT RPPX_22650 ← benzene 1,2‑dioxygenase
RA 5,157,352 C→T intergenic (+178/+65) RPPX_22875 → / ← dnaE2 integrase/DNA polymerase
MC JC 5,273,059 Δ2,603 bp RPPX_23450[RPPX_23460] RPPX_23450, RPPX_23455, [RPPX_23460]
RA 5,305,850 A→G I115V (ATA→GTA)  RPPX_23590 → leucyl‑tRNA synthetase
RA 5,306,590 (C)6→7 coding (801/972 nt) RPPX_23595 ← allophanate hydrolase
RA 5,426,639 T→C V332V (GTA→GTG RPPX_24160 ← damage‑inducible protein CinA
RA 5,513,866 G→A A266V (GCA→GTA)  RPPX_24600 ← integrase
RA 5,513,872 T→C Q264R (CAG→CGG)  RPPX_24600 ← integrase
RA 5,515,235 A→G intergenic (‑22/+120) RPPX_24605 ← / ← RPPX_24610 transposase/hypothetical protein
RA 5,595,953 T→C L225P (CTC→CCC)  RPPX_24915 → hypothetical protein
RA 5,597,121 C→T T614T (ACC→ACT RPPX_24915 → hypothetical protein
RA 5,597,130 T→C R617R (CGT→CGC RPPX_24915 → hypothetical protein
RA 5,602,532 Δ1 bp coding (258/2253 nt) RPPX_24935 → isoquinoline 1‑oxidoreductase
RA 5,672,449 G→A G827G (GGG→GGA RPPX_25245 → hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009974 341141–341386 342308 923–1168 221 [219] [215] 220 RPPX_01355/RPPX_01370 cytochrome C oxidase/cbb3‑type cytochrome c oxidase subunit I
* * ÷ CP009974 1155612 1155782 171 224 [219] [7] 445 RPPX_05115 hypothetical protein
* * ÷ CP009974 1156053 1156266 214 427 [22] [219] 223 RPPX_05120 hypothetical protein
* * ÷ CP009974 1156367 1157206 840 373 [36] [0] 802 [RPPX_05120] [RPPX_05120]
* * ÷ CP009974 1157290 1158132–1157501 212–843 223 [212] [219] 225 RPPX_05120/RPPX_05130 hypothetical protein/channel protein TolC
* * ÷ CP009974 1896592–1897071 1897158–1897127 57–567 222 [219] [208] 220 RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* * ÷ CP009974 5324682 5328660–5327261 2580–3979 337 [0] [218] 221 RPPX_23675–RPPX_23690 RPPX_23675,RPPX_23680,RPPX_23685,RPPX_23690
* * ÷ CP009974 5798527 5798534 8 252 [0] [0] NA RPPX_25885/– integrase/–

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009974 2596 =1 (0.000)239 (0.770) 152/284 1.5 99.8% intergenic (‑203/+211) RPPX_00010/RPPX_00015 integrase/peptidase S24
?CP009974 = 5798526 0 (0.000)intergenic (‑14/–) RPPX_25885/– integrase/–
* ? CP009974 884033 =127 (0.390)237 (0.970)
+38 bp
138/224 0.3 70.3% intergenic (+81/+252) RPPX_03845/RPPX_03850 FAD‑binding protein/general secretion pathway protein GspN
?CP009974 = 884084 142 (0.430)intergenic (+132/+201) RPPX_03845/RPPX_03850 FAD‑binding protein/general secretion pathway protein GspN
* ? CP009974 1154348 =NA (NA)240 (1.060)
+46 bp
107/208 1.8 56.9% coding (1048/2580 nt) RPPX_05115 hypothetical protein
?CP009974 = 1155588 263 (0.800)coding (2288/2580 nt) RPPX_05115 hypothetical protein
* ? CP009974 1154348 =NA (NA)252 (1.020)
+37 bp
123/226 1.2 58.5% coding (1048/2580 nt) RPPX_05115 hypothetical protein
?CP009974 = 1155597 238 (0.720)coding (2297/2580 nt) RPPX_05115 hypothetical protein
* ? CP009974 1154854 =312 (0.950)296 (1.260)
+43 bp
133/214 0.3 72.3% coding (1554/2580 nt) RPPX_05115 hypothetical protein
?CP009974 1155783 = 7 (0.020)coding (2483/2580 nt) RPPX_05115 hypothetical protein
* ? CP009974 = 1155303602 (1.830)320 (1.040) 169/282 0.5 53.1% coding (2003/2580 nt) RPPX_05115 hypothetical protein
?CP009974 = 1156366 0 (0.000)coding (453/564 nt) RPPX_05120 hypothetical protein
* ? CP009974 1155488 =372 (1.130)372 (1.280)
+AGGGTCCAGGGTGATGG
127/266 2.9 69.2% coding (2188/2580 nt) RPPX_05115 hypothetical protein
?CP009974 = 1156052 1 (0.000)coding (139/564 nt) RPPX_05120 hypothetical protein
* ? CP009974 1155977 =1 (0.000)385 (1.590)
+39 bp
137/222 0.3 72.5% coding (64/564 nt) RPPX_05120 hypothetical protein
?CP009974 = 1158685 395 (1.200)intergenic (+2208/‑13075) RPPX_05120/RPPX_05130 hypothetical protein/channel protein TolC
* ? CP009974 1157207 =0 (0.000)355 (1.980) 77/164 2.6 100% intergenic (+730/‑14553) RPPX_05120/RPPX_05130 hypothetical protein/channel protein TolC
?CP009974 = 1158025 NA (NA)intergenic (+1548/‑13735) RPPX_05120/RPPX_05130 hypothetical protein/channel protein TolC
* ? CP009974 1277572 =329 (1.000)269 (0.950) 142/258 1.2 48.5% intergenic (+41/+197) RPPX_05635/RPPX_05640 aldehyde dehydrogenase/mechanosensitive ion channel protein MscS
?CP009974 = 1277722 289 (1.020)intergenic (+191/+47) RPPX_05635/RPPX_05640 aldehyde dehydrogenase/mechanosensitive ion channel protein MscS
* ? CP009974 1296410 =343 (1.040)58 (0.970)
+122 bp
29/56 1.0 48.2% intergenic (+98/+134) RPPX_05720/RPPX_05725 chromosome partitioning protein ParA/CreA
?CP009974 = 1296432 344 (1.050)intergenic (+120/+112) RPPX_05720/RPPX_05725 chromosome partitioning protein ParA/CreA
* ? CP009974 = 15087600 (0.000)300 (0.960) 175/284 0.3 100% intergenic (+932/‑204) RPPX_06680/RPPX_06690 membrane protein/integrase
?CP009974 1511364 = 0 (0.000)intergenic (+113/‑364) RPPX_06695/RPPX_06700 ATPase AAA/HxlR family transcriptional regulator
* ? CP009974 1574780 =NA (NA)163 (0.750)
+50 bp
102/200 1.8 100% intergenic (‑136/+50) RPPX_07060/RPPX_07065 biotin‑‑protein ligase/5S ribosomal RNA
?CP009974 = 1915303 0 (0.000)intergenic (‑9/+117) RPPX_08540/RPPX_08545 5S ribosomal RNA/23S ribosomal RNA
* ? CP009974 = 16586120 (0.000)287 (0.900) 164/290 0.9 100% intergenic (‑17/‑64) RPPX_07420/RPPX_07425 acyl‑CoA dehydrogenase/transposase
?CP009974 1659808 = 0 (0.000)intergenic (+152/+271) RPPX_07425/RPPX_07430 transposase/pyrroloquinoline quinone biosynthesis protein PqqE
* ? CP009974 = 1897294NA (NA)1050 (3.300) 209/290 0.0 98.1% intergenic (‑5072/‑3525) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
?CP009974 1897952 = 21 (0.060)intergenic (‑5730/‑2867) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* ? CP009974 = 189730832 (0.100)715 (2.300) 137/284 2.8 97.6% intergenic (‑5086/‑3511) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
?CP009974 1897966 = 5 (0.020)intergenic (‑5744/‑2853) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* ? CP009974 1897973 =34 (0.100)787 (2.760) 174/260 0.1 96.4% intergenic (‑5751/‑2846) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
?CP009974 = 1898647 NA (NA)intergenic (‑6425/‑2172) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* ? CP009974 1898984 =172 (0.520)604 (2.740)
+49 bp
181/202 0.0 67.4% intergenic (‑6762/‑1835) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
?CP009974 = 1899234 697 (2.120)intergenic (‑7012/‑1585) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* ? CP009974 1898984 =172 (0.520)1051 (3.380)
+CGACTCGG
270/284 -0.0 81.9% intergenic (‑6762/‑1835) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
?CP009974 = 1899275 319 (0.970)intergenic (‑7053/‑1544) RPPX_08470/RPPX_08480 tryptophan synthase subunit beta/hypothetical protein
* ? CP009974 = 19015062 (0.010)337 (1.070) 189/286 0.1 99.7% coding (409/426 nt) RPPX_08485 hypothetical protein
?CP009974 1904110 = 0 (0.000)intergenic (+113/‑1737) RPPX_08495/RPPX_08505 ATPase AAA/hemolysin secretion protein D
* ? CP009974 = 27471174 (0.010)305 (0.980) 177/284 0.3 98.6% coding (76/867 nt) RPPX_12205 LysR family transcriptional regulator
?CP009974 2749721 = 5 (0.020)coding (99/216 nt) RPPX_12220 hypothetical protein
* ? CP009974 = 3063941NA (NA)852 (2.760)
+TTTTTCCTG
243/282 0.0 NA intergenic (+52/‑16) RPPX_13645/RPPX_13650 16S ribosomal RNA/tRNA‑Ile
?CP009974 3064156 = NA (NA)intergenic (+23/‑214) RPPX_13655/RPPX_13660 tRNA‑Ala/23S ribosomal RNA
* ? CP009974 = 3064033NA (NA)281 (0.880)
+ATATG
168/290 0.7 NA noncoding (77/77 nt) RPPX_13650 tRNA‑Ile
?CP009974 3064057 = NA (NA)intergenic (+24/‑1) RPPX_13650/RPPX_13655 tRNA‑Ile/tRNA‑Ala
* ? CP009974 = 3756397273 (0.830)237 (1.210)
+60 bp
122/180 0.1 62.5% intergenic (+279/+361) RPPX_16775/RPPX_16780 hypothetical protein/phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
?CP009974 4031306 = 204 (0.620)intergenic (+448/‑336) RPPX_17905/RPPX_17915 23S rRNA methyltransferase/sulfurtransferase
* ? CP009974 = 3844204269 (0.820)146 (0.770)
+63 bp
88/174 1.8 48.1% intergenic (+1/‑42) RPPX_17125/RPPX_17130 tRNA‑Ala/tRNA‑Glu
?CP009974 3844237 = 277 (0.840)intergenic (+34/‑9) RPPX_17125/RPPX_17130 tRNA‑Ala/tRNA‑Glu
* ? CP009974 = 53246810 (0.000)282 (0.900) 162/286 0.9 100% intergenic (+71/‑180) RPPX_23670/RPPX_23675 histidine kinase/integrase
?CP009974 5327261 = NA (NA)intergenic (+113/‑39) RPPX_23680/RPPX_23685 ATPase AAA/transposase