BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002094	305,914	C→T	K226K (AAG→AAA)	LLNZ_01670 ←	Nisin biosynthesis two‑component system, sensor histidine kinase NisK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002094	305,914	0	C	T	100.0%	418.4 / NA	103	K226K (AAG→AAA)	LLNZ_01670	Nisin biosynthesis two‑component system, sensor histidine kinase NisK
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (52/51); total (52/51)

Only 100 of 103 total aligned reads displayed.

GTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGCTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAATTTTTGATT  >  CP002094/305822‑306008

gTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCa                                                                                          <  2:822619/99‑1 (MQ=255)

gTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCa                                                                                          >  1:1532207/1‑99 (MQ=255)

 tCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCAt                                                                                         <  2:111622/99‑1 (MQ=255)

 tCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCAt                                                                                         <  1:1182486/99‑1 (MQ=255)

 tCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCAt                                                                                         >  2:629867/1‑99 (MQ=255)

 tCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCAt                                                                                         >  2:24541/1‑99 (MQ=255)

 tCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCAt                                                                                         >  1:1563184/1‑99 (MQ=255)

  cATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATg                                                                                        >  2:378263/1‑99 (MQ=255)

   aTCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGa                                                                                       <  1:1625361/99‑1 (MQ=255)

   aTCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGa                                                                                       <  1:575055/99‑1 (MQ=255)

    tctcTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGAc                                                                                      >  2:335363/1‑99 (MQ=255)

        tAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTc                                                                                  <  2:1274204/99‑1 (MQ=255)

        tAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTc                                                                                  <  1:898181/99‑1 (MQ=255)

          gCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCg                                                                                >  2:465763/1‑99 (MQ=255)

           cAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGc                                                                               <  2:1541092/99‑1 (MQ=255)

            aGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCt                                                                              >  1:1471149/1‑99 (MQ=255)

             gTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCtt                                                                             >  1:629142/1‑99 (MQ=255)

              ttCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTc                                                                            >  2:1576839/1‑99 (MQ=255)

                 aaTATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAAt                                                                         <  1:34193/99‑1 (MQ=255)

                 aaTATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAAt                                                                         <  2:1525809/99‑1 (MQ=255)

                  atatTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATa                                                                        >  1:1642192/1‑99 (MQ=255)

                  atatTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATa                                                                        <  2:24105/99‑1 (MQ=255)

                  atatTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATa                                                                        >  2:69746/1‑99 (MQ=255)

                   tatTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATaa                                                                       >  1:665591/1‑99 (MQ=255)

                   tatTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATaa                                                                       >  1:580055/1‑99 (MQ=255)

                     tttCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAgg                                                                     >  1:1359808/1‑99 (MQ=255)

                     tttCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAgg                                                                     >  2:1351064/1‑99 (MQ=255)

                      ttCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGa                                                                    <  1:1315970/99‑1 (MQ=255)

                       tCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGaa                                                                   >  2:703018/1‑99 (MQ=255)

                       tCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGaa                                                                   >  2:905925/1‑99 (MQ=255)

                         cTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAg                                                                 >  1:120959/1‑99 (MQ=255)

                         cTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAg                                                                 <  2:1631888/99‑1 (MQ=255)

                          ttttAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGc                                                                <  1:695170/99‑1 (MQ=255)

                          ttttAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGc                                                                <  2:451764/99‑1 (MQ=255)

                          ttttAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGc                                                                <  2:1110789/99‑1 (MQ=255)

                            ttAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAGTCATGTTTTTCATGACGCTCCGCTTCAATAAAGAAAGCTa                                                              >  1:1565514/1‑99 (MQ=255)

                            ttAATACTGTTAAAGGCGTCTTAACATCATGTGAAAGGGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTa                                                              >  2:813280/1‑99 (MQ=255)

                             taataaTGTAAAATGTTTATAAACAACAGGTGAAAGTGCAGCAACCGGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTaa                                                             <  1:1583847/99‑1 (MQ=255)

                               aTACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACt                                                           <  2:1545034/99‑1 (MQ=255)

                               aTACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACt                                                           <  1:24541/99‑1 (MQ=255)

                               aTACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACt                                                           >  1:439008/1‑99 (MQ=255)

                               aTACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACt                                                           >  1:1272175/1‑99 (MQ=255)

                                tACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTc                                                          >  2:339780/1‑99 (MQ=255)

                                tACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTc                                                          >  2:381095/1‑99 (MQ=255)

                                tACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTc                                                          >  2:636316/1‑99 (MQ=255)

                                tACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTc                                                          >  2:936051/1‑99 (MQ=255)

                                tACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAAGCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTc                                                          <  1:1086696/99‑1 (MQ=255)

                                 aCTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTcc                                                         >  1:720875/1‑99 (MQ=255)

                                  cTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCt                                                        <  2:37790/99‑1 (MQ=255)

                                    gTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTa                                                      >  2:1056963/1‑99 (MQ=255)

                                     ttAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTAc                                                     <  1:775204/99‑1 (MQ=255)

                                        aaGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACttt                                                  >  2:234915/1‑99 (MQ=255)

                                         aGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACtttt                                                 <  1:1121242/99‑1 (MQ=255)

                                         aGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACtttt                                                 <  2:82265/99‑1 (MQ=255)

                                          ggTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTc                                                <  2:597834/99‑1 (MQ=255)

                                            tgtCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGa                                              >  1:1470649/1‑99 (MQ=255)

                                             gtCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGaa                                             <  2:894122/99‑1 (MQ=255)

                                             gtCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGaa                                             <  2:1071570/99‑1 (MQ=255)

                                              tCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAat                                            >  2:48301/1‑99 (MQ=255)

                                               cTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAata                                           >  2:775717/1‑99 (MQ=255)

                                               cTTAACATCATGCGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAata                                           <  1:1255019/99‑1 (MQ=255)

                                                ttAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAatat                                          <  1:1488276/99‑1 (MQ=255)

                                                  aaCATCATGTGAAAGTGGAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATaa                                        <  1:994104/99‑1 (MQ=255)

                                                  aaCATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATaa                                        <  2:1382963/99‑1 (MQ=255)

                                                  aaCATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATaa                                        >  1:558109/1‑99 (MQ=255)

                                                   aCATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAg                                       >  2:1201524/1‑99 (MQ=255)

                                                    catcatGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGt                                      >  2:1655638/1‑99 (MQ=255)

                                                     atcatGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGtt                                     >  1:818588/1‑99 (MQ=255)

                                                      tcatGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTa                                    >  2:817086/1‑99 (MQ=255)

                                                      tcatGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTa                                    >  2:1065127/1‑99 (MQ=255)

                                                       catGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTAt                                   <  1:987298/99‑1 (MQ=255)

                                                          gtgAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCa                                >  1:919021/1‑99 (MQ=255)

                                                          gtgAAAGTGCAGCAACCTGGAAGAATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCa                                <  2:62642/99‑1 (MQ=255)

                                                           tgAAAGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAg                               <  1:1505725/99‑1 (MQ=255)

                                                             aaaGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGaa                             <  2:351608/99‑1 (MQ=255)

                                                              aaGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAAc                            <  1:844683/99‑1 (MQ=255)

                                                              aaGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAAc                            <  2:1631383/99‑1 (MQ=255)

                                                              aaGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAAc                            >  2:827742/1‑99 (MQ=255)

                                                               aGTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAAcc                           <  1:23717/99‑1 (MQ=255)

                                                                gTGCAGCAACCTGGAAGGATAAATCATGTTTTTCATGACTCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCt                          <  2:216831/99‑1 (MQ=255)

                                                                   cagcaACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGAt                       <  1:165832/99‑1 (MQ=255)

                                                                     gcaACCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCa                     >  2:387723/1‑99 (MQ=255)

                                                                       aaCCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCaaa                   <  1:1155830/99‑1 (MQ=255)

                                                                       aaCCTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCaaa                   >  2:1546751/1‑99 (MQ=255)

                                                                          cTGGAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTc                <  1:1351064/99‑1 (MQ=255)

                                                                            ggAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCaa              <  1:1655638/99‑1 (MQ=255)

                                                                             gAAGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCaat             <  1:387723/99‑1 (MQ=255)

                                                                               aGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCaataa           >  1:52065/1‑99 (MQ=255)

                                                                               aGGATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCaataa           >  2:1235809/1‑99 (MQ=255)

                                                                                ggATAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCaataat          >  1:157572/1‑99 (MQ=255)

                                                                                  aTAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAAttt        >  1:325967/1‑99 (MQ=255)

                                                                                  aTAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAAttt        <  1:191035/99‑1 (MQ=255)

                                                                                   tAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAAtttt       <  2:646655/99‑1 (MQ=255)

                                                                                   tAAATCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAAtttt       <  2:450077/99‑1 (MQ=255)

                                                                                     aaTCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAATTTTTg     >  1:1539047/1‑99 (MQ=255)

                                                                                     aaTCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAATTTTTg     <  2:868080/99‑1 (MQ=255)

                                                                                      aTCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAATTTTTGa    >  2:306233/1‑99 (MQ=255)

                                                                                       tCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAATTTTTGAt   >  1:897248/1‑99 (MQ=255)

                                                                                       tCATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAATTTTTGAt   <  2:1305704/99‑1 (MQ=255)

                                                                                        cATGTTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAATTTTTGAtt  <  1:1237174/99‑1 (MQ=255)

GTCATCTCTAGCAGTTCAATATTTCCTTTTAATACTGTTAAAGGTGTCTTAACATCATGTGAAAGTGCAGCAACCTGGAAGGATAAATCATGCTTTTCATGACGCTCCGCTTCAATAAGGAAAGCTAACTCCTTACTTTTCGAATATAAGTTATTCAGAACCTGATCAAATTCAATAATTTTTGATT  >  CP002094/305822‑306008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`