breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
JC 2,134,839 Δ5 bp coding (251‑255/1854 nt) asmA ← outer membrane assembly protein AsmA
MC JC 2,459,864 Δ10,215 bp intStorI 17 genes
RA 3,631,327 T→G L109R (CTG→CGG)  pitA → inorganic phosphate transporter PitA
RA 4,322,608 A→C V164G (GTG→GGG)  pmrB ← two‑component system sensor histidine kinase PmrB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 235454 270747–269666 34213–35294 134 [0] [57] 59 [BW25113_RS01100]–[BW25113_RS01325] [BW25113_RS01100],BW25113_RS25420,yafV,ivy,fadE,lpcA,yafJ,dpaA,yafQ,dinJ,yafL,rayT,BW25113_RS01160,lafU,dinB,yafN,yafO,yafP,BW25113_RS01190,prfH,pepD,gpt,frsA,crl,phoE,proB,proA,BW25113_RS01235,ykfI,yafW,ykfH,ykfG,yafX,ykfF,BW25113_RS01265,BW25113_RS25425,BW25113_RS25430,BW25113_RS25435,yafZ,ykfA,perR,BW25113_RS01295,BW25113_RS01300,BW25113_RS01305,ykfC,[BW25113_RS01325]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 2354530 (0.000)134 (1.290) 112/492 0.0 100% pseudogene (137/357 nt) BW25113_RS01100 hypothetical protein
?NZ_CP009273 269666 = NA (NA)intergenic (+2/+146) ykfC/BW25113_RS01325 reverse transcriptase domain‑containing protein/IS5‑like element IS5 family transposase
* ? NZ_CP009273 360104 =44 (0.420)67 (0.640) 59/492 1.8 56.1% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 61 (0.590)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =25 (0.240)76 (0.780) 72/460 0.6 77.0% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 22 (0.230)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326125 (0.240)52 (0.540) 48/460 2.6 69.6% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 22 (0.230)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 2457817 =8 (0.080)115 (1.110) 101/492 0.1 93.5% coding (670/756 nt) mlaA phospholipid‑binding lipoprotein MlaA
?NZ_CP009273 = 3577555 NA (NA)intergenic (+15/‑209) BW25113_RS17830/BW25113_RS25760 IS1‑like element IS1A family transposase/protein YrhD
* ? NZ_CP009273 = 24578262 (0.020)109 (1.050) 98/490 0.1 98.2% coding (661/756 nt) mlaA phospholipid‑binding lipoprotein MlaA
?NZ_CP009273 3576789 = NA (NA)coding (392/417 nt) BW25113_RS17825 YrhA family protein