breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,236,683 A→C M282R (ATG→AGG)  cvrA ← potassium/proton antiporter
RA 1,334,821 G→A E3K (GAG→AAG)  lapB → lipopolysaccharide assembly protein LapB
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 3,330,552 G→A Q22* (CAG→TAG)  mlaB ← lipid asymmetry maintenance protein MlaB
RA 4,323,610 C→A G53V (GGG→GTG)  basR ← two‑component system response regulator BasR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 275023–275637 286961–286346 10710–11939 40 [39] [39] 40 [BW25113_RS01350]–[BW25113_RS01405] [BW25113_RS01350],BW25113_RS01350,BW25113_RS23265,BW25113_RS01360,yagB,BW25113_RS26050,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,BW25113_RS23270,BW25113_RS01405,[BW25113_RS01405]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =39 (0.500)51 (0.660) 45/496 1.6 59.0% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 32 (0.410)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 2095230 =NA (NA)103 (1.320) 82/496 0.1 100% pseudogene (1/354 nt) wbbL N‑acetylglucosaminyl‑diphospho‑decaprenol L‑rhamnosyltransferase WbbL
?NZ_CP009273 2123121 = 0 (0.000)coding (11/747 nt) wcaE colanic acid biosynthesis glycosyltransferase WcaE
* ? NZ_CP009273 = 21231254 (0.050)89 (1.150) 65/494 0.4 95.7% coding (7/747 nt) wcaE colanic acid biosynthesis glycosyltransferase WcaE
?NZ_CP009273 = 2283591 NA (NA)intergenic (‑67/‑388) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 3576788 =NA (NA)93 (1.190) 84/496 0.0 94.9% coding (391/417 nt) BW25113_RS17825 YrhA family protein
?NZ_CP009273 = 3794204 5 (0.060)coding (122/699 nt) rfaY lipopolysaccharide core heptose(II) kinase RfaY
* ? NZ_CP009273 = 3577555NA (NA)83 (1.070) 74/496 0.1 95.4% intergenic (+15/‑209) BW25113_RS17830/BW25113_RS25760 IS1‑like element IS1A family transposase/protein YrhD
?NZ_CP009273 3794196 = 4 (0.050)coding (130/699 nt) rfaY lipopolysaccharide core heptose(II) kinase RfaY