breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 2,652,045 A→G S84P (TCT→CCT)  hscA ← Fe‑S protein assembly chaperone HscA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 275017–275641 286993–286348 10708–11977 51 [50] [49] 51 [BW25113_RS01350]–[BW25113_RS01405] [BW25113_RS01350],BW25113_RS01350,BW25113_RS23265,BW25113_RS01360,yagB,BW25113_RS26050,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,BW25113_RS23270,BW25113_RS01405,[BW25113_RS01405]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =51 (0.510)69 (0.690) 57/496 1.7 52.9% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 72 (0.720)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =12 (0.120)68 (0.730) 64/464 0.9 84.4% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 14 (0.150)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326112 (0.120)78 (0.840) 69/464 0.6 86.1% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 14 (0.150)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 2277591 =1 (0.010)131 (1.310) 101/496 0.1 99.2% intergenic (‑165/‑17) yejK/yejL nucleoid‑associated protein YejK/YejL family protein
?NZ_CP009273 2282398 = NA (NA)pseudogene (3/2532 nt) yejO autotransporter YejO
* ? NZ_CP009273 = 22775942 (0.020)127 (1.270) 98/496 0.1 98.5% intergenic (‑168/‑14) yejK/yejL nucleoid‑associated protein YejK/YejL family protein
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP