breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 659,964 T→G D228A (GAT→GCT)  rlpA ← endolytic peptidoglycan transglycosylase RlpA
RA 687,389 C→A E137* (GAA→TAA)  ybeY ← rRNA maturation RNase YbeY
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
JC 4,094,845 (TCAACA)1→2 coding (59/1374 nt) cpxA ← envelope stress sensor histidine kinase CpxA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 275007–275642 286989–286345 10704–11983 47 [46] [46] 47 [BW25113_RS01350]–[BW25113_RS01405] [BW25113_RS01350],BW25113_RS01350,BW25113_RS23265,BW25113_RS01360,yagB,BW25113_RS26050,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,BW25113_RS23270,BW25113_RS01405,[BW25113_RS01405]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =46 (0.520)54 (0.610) 48/496 2.0 54.5% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 44 (0.500)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 2654891 =4 (0.050)93 (1.050) 82/494 0.1 95.9% intergenic (‑1/+111) iscS/iscR cysteine desulfurase/Fe‑S cluster assembly transcriptional regulator IscR
?NZ_CP009273 3576788 = NA (NA)coding (391/417 nt) BW25113_RS17825 YrhA family protein
* ? NZ_CP009273 = 26548993 (0.030)85 (0.960) 75/496 0.3 96.6% intergenic (‑9/+103) iscS/iscR cysteine desulfurase/Fe‑S cluster assembly transcriptional regulator IscR
?NZ_CP009273 = 3577555 NA (NA)intergenic (+15/‑209) BW25113_RS17830/BW25113_RS25760 IS1‑like element IS1A family transposase/protein YrhD