BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,684	+A	coding (400/501 nt)	ytfI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,681	1	.	A	99.2%	1915.8 / 5.3	471	coding (397/501 nt)	ytfI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/2); new base A (240/227); total (242/229)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 518 total aligned reads displayed.

TGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑C‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGG  >  NC_000913/4435586‑4435771

tGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGAATTTCAAAAACCGTGAATTTCCGTTAGaa‑c                                                                                             <  1:14146672/96‑1 (MQ=255)

  cGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaa‑c‑aa                                                                                          >  2:9515515/1‑96 (MQ=255)

   gAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaa‑c‑aca                                                                                         >  1:6816895/1‑94 (MQ=255)

    aGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaa‑c‑aaaa                                                                                        <  1:2426185/96‑2 (MQ=255)

    aGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaa‑c‑aaaa                                                                                        >  1:1996265/1‑95 (MQ=255)

      gTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaa‑c‑aaaaca                                                                                      <  1:4997188/96‑4 (MQ=255)

      gTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaa‑c‑aaaaca                                                                                      >  2:4609975/1‑93 (MQ=255)

         gTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGAc                                                                                    <  1:3445812/96‑1 (MQ=255)

          tGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACg                                                                                   >  2:11004683/1‑96 (MQ=255)

           ggATTTAAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGa                                                                                  >  2:13719037/1‑96 (MQ=255)

            gATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGAt                                                                                 >  1:544880/1‑96 (MQ=255)

             aTTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTACCGTTAGAA‑CAAAACAGACGAtt                                                                                >  1:12563211/1‑96 (MQ=255)

              tttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTg                                                                               <  2:5403538/96‑1 (MQ=255)

               ttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGt                                                                              >  2:13116057/1‑96 (MQ=255)

                   aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTAttc                                                                          <  1:3095899/96‑3 (MQ=255)

                    tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGcc                                                                         <  2:4760027/96‑1 (MQ=255)

                     aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCt                                                                        >  1:740944/1‑96 (MQ=255)

                     aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCt                                                                        <  1:2783165/96‑1 (MQ=255)

                      cGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTg                                                                       <  1:9249624/96‑1 (MQ=255)

                      cGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTg                                                                       <  1:6416694/96‑1 (MQ=255)

                       gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTgg                                                                      >  2:4946416/1‑96 (MQ=255)

                         agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGa                                                                    <  2:6745924/96‑1 (MQ=255)

                         agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGa                                                                    >  1:13243757/1‑96 (MQ=255)

                          gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAACCCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGat                                                                   <  2:223698/96‑1 (MQ=255)

                          gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGat                                                                   >  2:413014/1‑96 (MQ=255)

                            aaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGatat                                                                 <  2:3469159/96‑1 (MQ=255)

                             aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGatact                                                                <  1:11784123/96‑3 (MQ=255)

                             aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATAtt                                                                >  2:5200302/1‑96 (MQ=255)

                             aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGACAtt                                                                <  2:10107161/96‑1 (MQ=255)

                              tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTc                                                               >  1:14349977/1‑96 (MQ=255)

                              tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTc                                                               <  1:11004683/96‑1 (MQ=255)

                              tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTc                                                               >  1:1382206/1‑96 (MQ=255)

                              tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTc                                                               >  2:895320/1‑96 (MQ=255)

                               aCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCt                                                              <  2:6608209/96‑1 (MQ=255)

                                cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTa                                                             >  1:4061926/1‑96 (MQ=255)

                                cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTa                                                             <  2:9301618/96‑1 (MQ=255)

                                 ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTAc                                                            <  2:3978345/96‑1 (MQ=255)

                                 ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTAc                                                            >  2:6894028/1‑96 (MQ=255)

                                    tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGa                                                         >  2:2629442/1‑96 (MQ=255)

                                     aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGaa                                                        >  1:3615275/1‑96 (MQ=255)

                                      aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaa                                                       >  1:11163721/1‑96 (MQ=255)

                                          tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                   <  1:252920/96‑1 (MQ=255)

                                           aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                  <  1:7105488/96‑1 (MQ=255)

                                           aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                  >  1:13008385/1‑96 (MQ=255)

                                             aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGt                                                >  1:13568027/1‑96 (MQ=255)

                                              tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGGATGCCTGGGATATTCTACAGAAAACACAGTc                                               >  1:1205793/1‑96 (MQ=255)

                                               gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                              >  1:2134070/1‑96 (MQ=255)

                                               gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                              <  2:4513248/96‑1 (MQ=255)

                                                 atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                            <  2:8392089/96‑1 (MQ=255)

                                                  tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTgcc                                           <  1:11452154/96‑3 (MQ=255)

                                                  tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                           >  2:6388634/1‑96 (MQ=255)

                                                  tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCAGAc                                           >  1:10027850/1‑96 (MQ=255)

                                                    taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                         <  2:9837148/96‑1 (MQ=255)

                                                     aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                        >  2:9043437/1‑96 (MQ=255)

                                                     aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                        >  1:6713556/1‑96 (MQ=255)

                                                     aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                        >  1:943946/1‑96 (MQ=255)

                                                      aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                       >  1:9086403/1‑96 (MQ=255)

                                                      aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                       <  2:1211894/96‑1 (MQ=255)

                                                        tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATCCCg                                     >  2:1393397/1‑96 (MQ=255)

                                                        tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                     >  2:10800854/1‑96 (MQ=255)

                                                        tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                     >  2:908131/1‑96 (MQ=255)

                                                        tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                     <  2:5690242/96‑1 (MQ=255)

                                                         cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                    <  2:11223619/96‑1 (MQ=255)

                                                         cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACACACCGa                                    >  2:12730705/1‑96 (MQ=255)

                                                         cattcatGGATTTCAAAAACCGGGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                    >  2:3027374/1‑96 (MQ=255)

                                                           ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                  <  2:10389353/96‑1 (MQ=255)

                                                              atGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAACCAGTCTGACATACCGAAAAgg                               <  2:11932360/96‑1 (MQ=255)

                                                              atGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                               >  1:3329305/1‑96 (MQ=255)

                                                               tGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                              <  2:1948926/96‑1 (MQ=255)

                                                                ggATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                             <  2:2071997/96‑1 (MQ=255)

                                                                ggATTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                             <  1:7719919/96‑1 (MQ=255)

                                                                 gATTTCAAAAACCGTGAATTTCCGTTAGAATA‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGagt                            <  1:10280701/96‑3 (MQ=255)

                                                                  aTTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa                           <  2:11846544/96‑1 (MQ=255)

                                                                  aTTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa                           <  1:5358193/96‑1 (MQ=255)

                                                                  aTTTCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa                           <  2:10532627/96‑1 (MQ=255)

                                                                  aTTTCAAAAACCGGGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa                           >  1:13446672/1‑96 (MQ=255)

                                                                    ttCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                         <  1:8281485/96‑1 (MQ=255)

                                                                    ttCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                         >  2:5936221/1‑96 (MQ=255)

                                                                     tCAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                        >  1:184879/1‑96 (MQ=255)

                                                                      cAAAAACCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                       >  2:3841413/1‑96 (MQ=255)

                                                                        aaaaCCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATACTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAggct                     <  1:10559582/96‑3 (MQ=255)

                                                                         aaaCCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                    <  2:2840745/96‑1 (MQ=255)

                                                                         aaaCCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                    >  2:11523171/1‑96 (MQ=255)

                                                                          aaCCGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                   >  1:4753758/1‑96 (MQ=255)

                                                                          aaCCGTGAATTTCCGTTAGAA‑CAAAACAGAAGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                   <  2:4293636/96‑1 (MQ=255)

                                                                            ccGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat                 >  2:13444938/1‑96 (MQ=255)

                                                                            ccGTGAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat                 <  1:5849589/96‑1 (MQ=255)

                                                                                gAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTccc             <  1:10730075/96‑3 (MQ=255)

                                                                                gAATTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc             >  1:6104715/1‑96 (MQ=255)

                                                                                 aaTTTCCGTTAGAA‑CAAAACAGACGATTGTATTCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCCAAAAGGATCAAAGGTTATATTCAcc            >  2:14393231/1‑96 (MQ=255)

                                                                                 aaTTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc            <  1:7974267/96‑1 (MQ=255)

                                                                                 aaTTTCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc            >  2:737556/1‑96 (MQ=255)

                                                                                   tttCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg          >  1:12261626/1‑96 (MQ=255)

                                                                                   tttCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg          <  2:12467356/96‑1 (MQ=255)

                                                                                   tttCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg          <  1:4249253/96‑1 (MQ=255)

                                                                                    ttCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAgg         <  2:7157903/96‑1 (MQ=255)

                                                                                     tCCGTTAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc        <  1:3827564/96‑1 (MQ=255)

                                                                                          tAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTg   >  2:7971602/1‑96 (MQ=255)

                                                                                          tAGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTg   <  1:4609975/96‑1 (MQ=255)

                                                                                           aGAA‑CAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTgg  <  1:12759404/96‑1 (MQ=255)

TGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA‑C‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGG  >  NC_000913/4435586‑4435771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`