Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,449,738 +G coding (1106/1254 nt) ytfR → YtfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,449,7371.G99.3% 1705.1 / 1.4 446coding (1105/1254 nt)ytfRYtfR
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (241/202);  total (243/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 483 total aligned reads displayed.

TTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC  >  NC_000913/4449642‑4449827
                                                                                                 |                                                                                          
ttGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t                                                                                             >  1:2890525/1‑96 (MQ=255)
  ggCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑gg                                                                                          <  2:14403549/96‑2 (MQ=255)
  ggCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑gg                                                                                          >  1:4504407/1‑95 (MQ=255)
    cTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑ggct                                                                                        <  2:10410184/96‑4 (MQ=255)
    cTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑ggct                                                                                        <  2:9877702/96‑4 (MQ=255)
     ttCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑ggcta                                                                                       <  2:2813253/96‑5 (MQ=255)
       cTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACg                                                                                      <  2:6929057/96‑1 (MQ=255)
         gCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTc                                                                                    >  2:3679458/1‑96 (MQ=255)
          cccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTcc                                                                                   >  2:13636733/1‑96 (MQ=255)
           ccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTccc                                                                                  >  2:2243619/1‑96 (MQ=255)
            cGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCa                                                                                 >  2:2398414/1‑96 (MQ=255)
             ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCAt                                                                                >  1:8346292/1‑96 (MQ=255)
             ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCAt                                                                                >  2:9261417/1‑96 (MQ=255)
                aGACAGGAAAACCAATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTc                                                                             <  2:10265906/96‑1 (MQ=255)
                 gACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTcc                                                                            >  1:3995855/1‑96 (MQ=255)
                  aCAGGAAAGCCGATGGCATCCTGGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCGGCGCGGC‑TGGCTACGTCCCCCTTccc                                                                           <  1:3345197/96‑1 (MQ=255)
                   cAGGAAAACCGATTGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCg                                                                          >  1:13900260/1‑96 (MQ=255)
                   cAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCg                                                                          <  2:9415059/96‑1 (MQ=255)
                   cAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCg                                                                          <  1:14136139/96‑1 (MQ=255)
                    aGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGc                                                                         <  2:9495198/96‑1 (MQ=255)
                    aGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGc                                                                         >  1:8714842/1‑96 (MQ=255)
                     ggAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCa                                                                        <  1:14401803/96‑1 (MQ=255)
                      gAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCaa                                                                       >  1:13385465/1‑96 (MQ=255)
                         aaCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGa                                                                    >  1:2496551/1‑96 (MQ=255)
                         aaCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGa                                                                    >  1:7840281/1‑96 (MQ=255)
                           ccGATGGCATCCTCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAAc                                                                  <  1:11621213/96‑1 (MQ=255)
                           ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAAc                                                                  <  1:9726010/96‑1 (MQ=255)
                            cGATGGCATCGTCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACa                                                                 <  1:7203000/96‑1 (MQ=255)
                             gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAg                                                                >  1:10031976/1‑96 (MQ=255)
                             gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAg                                                                >  1:3629581/1‑96 (MQ=255)
                             gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGGCCCATTTCCCGCAAAGAACAg                                                                >  2:6000674/1‑96 (MQ=255)
                               tGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCa                                                              <  2:169350/96‑1 (MQ=255)
                                 gCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                            <  1:5339291/96‑1 (MQ=255)
                                    tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                         <  2:2976779/96‑1 (MQ=255)
                                     catcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAGGAACAGCAAGAGAt                                                        >  1:3076074/1‑96 (MQ=255)
                                     catcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                        >  2:10042583/1‑96 (MQ=255)
                                      atcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGGtt                                                       >  2:3406439/1‑96 (MQ=255)
                                       tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTg                                                      <  1:7614221/96‑1 (MQ=255)
                                        cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGc                                                     >  2:4720079/1‑96 (MQ=255)
                                         gCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGcc                                                    >  2:11352536/1‑96 (MQ=255)
                                           tGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGa                                                  >  2:10749722/1‑96 (MQ=255)
                                            gccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                                 <  2:8905105/96‑1 (MQ=255)
                                            gccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                                 >  2:7357525/1‑96 (MQ=255)
                                            gccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAGTGCCGaa                                                 >  2:8422641/1‑96 (MQ=255)
                                             ccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGACTGCCGAAc                                                <  2:12956955/96‑1 (MQ=255)
                                             ccgccTCGGTGAGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCTGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                                <  1:12385585/96‑1 (MQ=255)
                                              cgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCAATTCCCGCAAAGAAGAGCAATATATTGTCGAACg                                               >  1:2795225/1‑96 (MQ=255)
                                              cgccTCGGTGCGGGAAAATATCATCCTCGCACTCCAGGCCTAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACg                                               <  2:11626403/96‑1 (MQ=255)
                                                ccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCt                                             >  2:6344792/1‑96 (MQ=255)
                                                 cTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCtt                                            >  1:5002845/1‑96 (MQ=255)
                                                   cGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                          >  1:8989494/1‑96 (MQ=255)
                                                    ggTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAt                                         >  2:4188171/1‑96 (MQ=255)
                                                     gTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                        >  1:13008622/1‑96 (MQ=255)
                                                      tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                       <  1:6563845/96‑1 (MQ=255)
                                                      tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTGTATct                                       >  1:6841276/1‑95 (MQ=255)
                                                       gCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCg                                      >  1:13288045/1‑96 (MQ=255)
                                                       gCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCg                                      >  1:13632753/1‑96 (MQ=255)
                                                         gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCGGTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGGTTTATCCGcc                                    >  2:14012848/1‑96 (MQ=255)
                                                         gcgAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGcc                                    <  1:10406372/94‑1 (MQ=255)
                                                           gAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAg                                  >  2:4932842/1‑96 (MQ=255)
                                                           gAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAg                                  <  2:3795227/96‑1 (MQ=255)
                                                            aaaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGc                                 >  2:8963372/1‑96 (MQ=255)
                                                             aaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCt                                >  1:7500976/1‑96 (MQ=255)
                                                               atatCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTg                              <  1:6199437/96‑1 (MQ=255)
                                                                tatCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTgg                             >  2:7172895/1‑96 (MQ=255)
                                                                   catcCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAt                          >  2:7825467/1‑96 (MQ=255)
                                                                     tcCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTc                        <  1:6310441/96‑1 (MQ=255)
                                                                      ccTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCg                       >  1:2220007/1‑96 (MQ=255)
                                                                       cTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGc                      <  1:5277161/96‑1 (MQ=255)
                                                                       cTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGc                      >  2:7597770/1‑96 (MQ=255)
                                                                        tCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                     >  1:1803825/1‑96 (MQ=255)
                                                                          gCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcaca                   >  2:13878466/1‑96 (MQ=255)
                                                                          gCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcaca                   >  2:3966134/1‑96 (MQ=255)
                                                                          gCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcaca                   >  2:14078158/1‑96 (MQ=255)
                                                                           ctctCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCacac                  <  1:11995655/96‑1 (MQ=255)
                                                                            tctcCAGGCCCAGCGCGGCGG‑GCTACGTCCCATTTCCCGCAAAGAACAACAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCCCACAcc                 >  2:12778040/1‑96 (MQ=255)
                                                                            tctcCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc                 >  2:4416108/1‑96 (MQ=255)
                                                                            tctcCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc                 >  1:2064102/1‑96 (MQ=255)
                                                                            tctcCAGGCCCAGCGCGGC‑TGGCTACGCCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGTTTTATCCGCCAGCTTGGCATTCGCACAcc                 <  1:5561208/96‑1 (MQ=255)
                                                                            accccAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc                 <  1:6048899/93‑1 (MQ=255)
                                                                              tcCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCtt               >  2:2570441/1‑96 (MQ=255)
                                                                              tcCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCtt               <  2:10510860/96‑1 (MQ=255)
                                                                               ccAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTc              >  2:12569850/1‑96 (MQ=255)
                                                                               ccAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTc              >  1:1057659/1‑96 (MQ=255)
                                                                                cAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCa             <  2:11938121/96‑1 (MQ=255)
                                                                                cAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCa             >  2:12250847/1‑96 (MQ=255)
                                                                                   gCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt          <  1:8248347/96‑1 (MQ=255)
                                                                                    cccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTTCCGAAGGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTg         >  2:8546904/1‑96 (MQ=255)
                                                                                    cccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGGACCCCTTCAACTg         <  1:10970568/96‑1 (MQ=255)
                                                                                    cccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTg         >  2:5176744/1‑96 (MQ=255)
                                                                                    cccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAAAAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAATg         >  2:13204150/1‑96 (MQ=255)
                                                                                     ccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCTAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa        >  1:9336775/1‑96 (MQ=255)
                                                                                     ccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa        >  2:2711717/1‑96 (MQ=255)
                                                                                        gcgcGGC‑TGGCTGCGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaaca     <  1:4271137/96‑1 (MQ=255)
                                                                                         cgcgGCTT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCCCACCTTCAACTGaacaa    <  1:1649828/96‑1 (MQ=255)
                                                                                         cgcgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaacaa    >  1:10310608/1‑96 (MQ=255)
                                                                                         cgcgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaacaa    <  2:11115140/96‑1 (MQ=255)
                                                                                          gcgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaacaac   >  2:10005775/1‑96 (MQ=255)
                                                                                          gcgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaacaac   >  2:5580219/1‑96 (MQ=255)
                                                                                           cgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAAcc  <  2:8714842/96‑1 (MQ=255)
                                                                                                 |                                                                                          
TTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC  >  NC_000913/4449642‑4449827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑