BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,650,355	T→C	F274L (TTC→CTC)	b1579 →	putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,650,355	0	T	C	99.9%	2676.1 / NA	657	F274L (TTC→CTC)	b1579	putative transposase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (253/402); total (254/403)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 662 total aligned reads displayed.

CGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT  >  NC_000913/1650262‑1650447

cGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTc                                                                                            <  1:6906193/96‑1 (MQ=255)

 gggTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCa                                                                                           >  1:3819423/1‑96 (MQ=255)

 gggTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCa                                                                                           <  2:8215092/96‑1 (MQ=255)

   gTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCaga                                                                                         <  2:2544153/96‑1 (MQ=255)

    tcattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCagag                                                                                        >  2:3308914/1‑96 (MQ=255)

     cattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCagaga                                                                                       <  1:6531055/96‑1 (MQ=255)

     cattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCagaga                                                                                       <  2:736365/96‑1 (MQ=255)

     cattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCagaga                                                                                       <  1:13653865/96‑1 (MQ=255)

       ttcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGt                                                                                     <  2:13104429/96‑1 (MQ=255)

       ttcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGt                                                                                     <  1:9589207/96‑1 (MQ=255)

        tcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTa                                                                                    <  2:4135708/96‑1 (MQ=255)

           tCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATgg                                                                                 >  2:931496/1‑96 (MQ=255)

           tCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATgg                                                                                 >  1:3684458/1‑96 (MQ=255)

           tCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATgg                                                                                 <  1:14263235/96‑1 (MQ=255)

            cTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGc                                                                                <  1:13895430/96‑1 (MQ=255)

            cTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGc                                                                                >  2:3455016/1‑96 (MQ=255)

             ttattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGca                                                                               <  1:10884266/96‑1 (MQ=255)

              tattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcag                                                                              <  1:12411985/96‑1 (MQ=255)

               attaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcaga                                                                             <  1:3475979/96‑1 (MQ=255)

                ttaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaa                                                                            <  2:310657/96‑1 (MQ=255)

                 taAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaac                                                                           >  2:699584/1‑96 (MQ=255)

                 taAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaac                                                                           <  1:929694/96‑1 (MQ=255)

                 taAGCCAGCAATCGACGCCCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCCCCTCAGAGAGTATGGcagaac                                                                           <  1:4088827/96‑1 (MQ=255)

                    gCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaacaga                                                                        <  1:10002110/96‑1 (MQ=255)

                    gCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGAATATCATTGATGTTCACCTCAGAGAGTATGGcagaacaga                                                                        >  2:4450903/1‑96 (MQ=255)

                     ccAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaacagaa                                                                       <  2:10130856/96‑1 (MQ=255)

                      cAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaa                                                                      <  2:5444379/96‑1 (MQ=255)

                      cAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaa                                                                      <  1:2057176/96‑1 (MQ=255)

                        gCAATCGACGCTCTCCGGAGTCAGATGAGATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGaaaaaaaaaa                                                                    >  2:13902994/1‑96 (MQ=255)

                          aaTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACa                                                                  >  2:6563025/1‑96 (MQ=255)

                          aaTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACa                                                                  >  1:5338580/1‑96 (MQ=255)

                            tCGACGCTCTCCGGAGCCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGATAGTATGGCAGAACATAAAAACaaa                                                                >  1:5609719/1‑96 (MQ=255)

                             cGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaaa                                                               >  2:7722429/1‑96 (MQ=255)

                             cGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaaa                                                               >  2:2788108/1‑96 (MQ=255)

                              naCGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaaaa                                                              >  1:3196123/2‑96 (MQ=255)

                              gACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaaaa                                                              >  1:2570908/1‑96 (MQ=255)

                              gACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaaaa                                                              >  2:5051629/1‑96 (MQ=255)

                               aCGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAt                                                             >  2:10802878/1‑96 (MQ=255)

                                 gCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGc                                                           <  2:9471708/96‑1 (MQ=255)

                                 gCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGc                                                           >  1:8147294/1‑96 (MQ=255)

                                  ctctCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCa                                                          >  1:7471985/1‑96 (MQ=255)

                                   tctcCGGAGTCAGATGACATTAACGAGACTGAGCACAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGctc                                                         <  1:10640728/96‑3 (MQ=255)

                                    ctcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCAcc                                                        >  2:1894632/1‑96 (MQ=255)

                                     tcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCt                                                       >  1:11924854/1‑96 (MQ=255)

                                       cGGAGTCAGATGACATTAACGAGCCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCtgt                                                     <  1:10155465/96‑3 (MQ=255)

                                       cGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCttt                                                     <  1:11603399/96‑1 (MQ=255)

                                        ggAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTg                                                    <  2:9733871/96‑1 (MQ=255)

                                        ggAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTg                                                    <  1:5201453/96‑1 (MQ=255)

                                         gAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGt                                                   <  1:6210905/96‑1 (MQ=255)

                                          aGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGtt                                                  >  2:6248421/1‑96 (MQ=255)

                                            tCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGtttt                                                <  2:3705510/96‑1 (MQ=255)

                                            tCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAACTCAGAGAGTATGGGAGAACAAAAAAACAAAAAGGCATCTTTGttct                                                >  1:8700673/1‑94 (MQ=255)

                                             cAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGttttt                                               <  1:7146765/96‑1 (MQ=255)

                                              aGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTc                                              <  1:2842138/96‑1 (MQ=255)

                                               gATGACATTAAGGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCa                                             >  2:8696493/1‑96 (MQ=255)

                                                 tGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAAc                                           <  2:14243897/96‑1 (MQ=255)

                                                    cATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTg                                        <  1:3327000/96‑1 (MQ=255)

                                                    cATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTg                                        >  1:7251747/1‑96 (MQ=255)

                                                    cATTAACGAGACTGAGCAAAGAGCATAGCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCGACCTg                                        >  2:12724605/1‑96 (MQ=255)

                                                     aTTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGa                                       >  1:4304467/1‑96 (MQ=255)

                                                      taaaCGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGaa                                      >  1:11473300/3‑96 (MQ=255)

                                                        aaCGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgt                                    >  2:4020092/1‑96 (MQ=255)

                                                         aCGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAgtg                                   >  2:7975826/1‑96 (MQ=255)

                                                           gagaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTc                                 <  1:13127417/96‑1 (MQ=255)

                                                           gagaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTc                                 <  2:6447994/96‑1 (MQ=255)

                                                           gagaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTc                                 <  2:1480705/96‑1 (MQ=255)

                                                           gagaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTc                                 <  2:10528355/96‑1 (MQ=255)

                                                            agaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGGCa                                >  2:2112497/1‑96 (MQ=255)

                                                            agaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTAAACCTGAAGTGTCa                                >  2:11310590/1‑96 (MQ=255)

                                                              aCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGc                              <  1:8489320/96‑1 (MQ=255)

                                                              aCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGc                              >  2:8892360/1‑96 (MQ=255)

                                                               cTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCg                             <  2:13320952/96‑1 (MQ=255)

                                                               cTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCg                             <  1:7821610/96‑1 (MQ=255)

                                                               cTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCg                             >  2:5874719/1‑96 (MQ=255)

                                                                tGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCga                            <  2:4645230/96‑1 (MQ=255)

                                                                 gAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCgag                           <  2:6704382/96‑1 (MQ=255)

                                                                  aGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCgaga                          >  1:1627903/1‑96 (MQ=255)

                                                                   gCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGagag                         >  2:11153404/1‑96 (MQ=255)

                                                                    cAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGt                        <  1:3070396/96‑1 (MQ=255)

                                                                    cAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGt                        >  1:4767176/1‑96 (MQ=255)

                                                                    cAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGt                        <  1:9135950/96‑1 (MQ=255)

                                                                     aaaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTa                       <  2:12280712/96‑1 (MQ=255)

                                                                      aaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAACGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaa                      <  1:12642726/96‑1 (MQ=255)

                                                                       agagCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCACCCTGAAGTGTCAGCGAGAGTaaa                     >  2:12633424/1‑96 (MQ=255)

                                                                       agagCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaa                     <  2:11884796/96‑1 (MQ=255)

                                                                        gagCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACTAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaa                    >  2:527673/1‑96 (MQ=255)

                                                                          gCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaaaa                  >  1:4958108/1‑96 (MQ=255)

                                                                          gCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaaaa                  >  2:13620888/1‑96 (MQ=255)

                                                                            atatCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAtt                >  2:855947/1‑96 (MQ=255)

                                                                              atCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAt              <  1:8889715/96‑1 (MQ=255)

                                                                              atCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAt              <  2:11559939/96‑1 (MQ=255)

                                                                               tCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATg             <  2:4912192/96‑1 (MQ=255)

                                                                               tCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATg             >  1:11248116/1‑96 (MQ=255)

                                                                                 aTTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGt           <  1:5978998/96‑1 (MQ=255)

                                                                                 aTTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGt           >  2:9108236/1‑96 (MQ=255)

                                                                                  ttGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCGGAAGTGTCAGCGAGAGTAAAAAATTATGGTg          <  1:12187157/96‑1 (MQ=255)

                                                                                     aTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAcc       >  2:1683205/1‑96 (MQ=255)

                                                                                      tGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGGAAAAAATTATGGGGACCa      >  2:5599781/1‑96 (MQ=255)

                                                                                          cACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCAtttt  <  1:2194282/96‑1 (MQ=255)

                                                                                          cACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCAtttt  <  1:10425044/96‑1 (MQ=255)

CGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTT  >  NC_000913/1650262‑1650447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`