Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 25,037 A→G S883G (AGC→GGC)  ileS → isoleucine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091325,0370AG82.8% 568.7 / 114.5 244S883G (AGC→GGC) ileSisoleucine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (27/15);  new base G (101/101);  total (128/116)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

Only 100 of 245 total aligned reads displayed.

ACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGG  >  NC_000913/24947‑25125
                                                                                          |                                                                                        
 ccGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCg                                                                                       >  2:3402018/1‑93 (MQ=255)
   gcgcTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGaa                                                                                     >  1:5742959/1‑93 (MQ=255)
    cgcTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAg                                                                                    <  1:2801844/93‑1 (MQ=255)
     gcTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGt                                                                                   >  2:366387/1‑93 (MQ=255)
     gcTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGt                                                                                   <  2:3355062/93‑1 (MQ=255)
     gcTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGt                                                                                   >  2:6463599/1‑93 (MQ=255)
      cTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTa                                                                                  >  2:4289060/1‑93 (MQ=255)
       tGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCCGCAGGGCGAAGTAc                                                                                 <  1:5177999/93‑1 (MQ=255)
       tGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTAc                                                                                 <  1:6378177/93‑1 (MQ=255)
       tGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTAc                                                                                 <  1:4807265/93‑1 (MQ=255)
       tGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTAc                                                                                 >  2:6010780/1‑93 (MQ=255)
        gggCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACt                                                                                >  1:1035771/1‑93 (MQ=255)
         ggCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTc                                                                               <  1:1741799/93‑1 (MQ=255)
            gATGAATTACGATTTGTCCTGTTGCCCTCCGGCGCTACCTTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCaaa                                                                            <  1:2768115/93‑1 (MQ=255)
            gATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCaaa                                                                            <  1:2488399/93‑1 (MQ=255)
            gATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCaaa                                                                            >  1:6558674/1‑93 (MQ=255)
            gATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCaaa                                                                            >  1:3128278/1‑93 (MQ=255)
             aTGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAg                                                                           >  1:1323168/1‑93 (MQ=255)
              tGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAgg                                                                          >  1:5790331/1‑93 (MQ=255)
              tGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAgg                                                                          <  1:5389327/93‑1 (MQ=255)
              tGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAgg                                                                          >  1:48986/1‑93 (MQ=255)
               gAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAggg                                                                         >  2:3960879/1‑93 (MQ=255)
                aaTTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGc                                                                        <  2:5813666/93‑1 (MQ=255)
                 aTTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCt                                                                       <  2:2084600/93‑1 (MQ=255)
                  ttACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTg                                                                      <  2:1099878/93‑1 (MQ=255)
                   tACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGa                                                                     <  2:4252878/93‑1 (MQ=255)
                      gATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAg                                                                  >  1:995693/1‑93 (MQ=255)
                       aTTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGt                                                                 >  1:2557230/1‑93 (MQ=255)
                       aTTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGt                                                                 >  2:5749728/1‑93 (MQ=255)
                        tttGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTc                                                                <  2:4445774/93‑1 (MQ=255)
                         ttGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTcg                                                               <  2:4129765/93‑1 (MQ=255)
                         ttGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTcg                                                               <  1:4050120/93‑1 (MQ=255)
                         ttGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTcg                                                               >  2:3825792/1‑93 (MQ=255)
                          tGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTcgc                                                              <  2:1006456/93‑1 (MQ=255)
                           gTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTcgcg                                                             <  2:3517198/93‑1 (MQ=255)
                           gTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTcgcg                                                             <  2:517998/93‑1 (MQ=255)
                            tCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGt                                                            <  2:1897018/93‑1 (MQ=255)
                            tCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGt                                                            <  1:1834060/93‑1 (MQ=255)
                             ccTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGtt                                                           >  1:2345719/1‑93 (MQ=255)
                              cTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTg                                                          >  2:454214/1‑93 (MQ=255)
                               tGTTGACCTCCGGCTCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGa                                                         >  2:4155744/1‑93 (MQ=255)
                                gTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAg                                                        >  1:3284812/1‑93 (MQ=255)
                                  tGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTa                                                      <  1:159213/93‑1 (MQ=255)
                                  tGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTa                                                      <  2:268765/93‑1 (MQ=255)
                                  tGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTa                                                      <  1:3352222/93‑1 (MQ=255)
                                    aCCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTaaa                                                    <  1:2420681/93‑1 (MQ=255)
                                    aCCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTaaa                                                    >  1:508296/1‑93 (MQ=255)
                                     ccTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAg                                                   >  2:5154210/1‑93 (MQ=255)
                                     ccTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAg                                                   <  1:4188482/93‑1 (MQ=255)
                                      cTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTaaacc                                                  <  1:5275758/93‑3 (MQ=255)
                                      cTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGc                                                  >  1:3046031/1‑93 (MQ=255)
                                      cTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGc                                                  <  2:1318207/93‑1 (MQ=255)
                                      cTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGCTGAGTAAAGc                                                  >  1:3295914/1‑93 (MQ=255)
                                       tCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGcc                                                 <  2:2010537/93‑1 (MQ=255)
                                        ccGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCg                                                >  2:4557403/1‑93 (MQ=255)
                                          ggCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGaa                                              <  1:6343759/93‑1 (MQ=255)
                                          ggCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGaa                                              <  1:429229/93‑1 (MQ=255)
                                           gcgcTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAg                                             >  1:4625801/1‑93 (MQ=255)
                                            cgcTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAgg                                            <  1:2708591/93‑1 (MQ=255)
                                             gcTCCCGTTGCAGACTATAACGACGCACGTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGt                                           <  2:3270677/93‑1 (MQ=255)
                                             gcTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGt                                           <  2:2262555/93‑1 (MQ=255)
                                             gcTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGt                                           >  1:2933605/1‑93 (MQ=255)
                                              ctcccGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTg                                          <  1:1639128/90‑1 (MQ=255)
                                               tACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTga                                         <  1:5463015/93‑1 (MQ=255)
                                                aCCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTgag                                        <  1:4532242/93‑1 (MQ=255)
                                                aCCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTgag                                        >  2:799544/1‑93 (MQ=255)
                                                 ccGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTgaga                                       <  2:133243/93‑1 (MQ=255)
                                                  cGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGaa                                      <  2:6328365/93‑1 (MQ=255)
                                                  cGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGCTGAGTAAAGCCGAAGGTGAGaa                                      >  2:4757328/1‑93 (MQ=255)
                                                      gCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGc                                  <  1:4765478/93‑1 (MQ=255)
                                                      gCAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGc                                  >  2:303829/1‑93 (MQ=255)
                                                       cAGACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGcc                                 <  1:4113955/93‑1 (MQ=255)
                                                        aGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGccc                                >  1:3609078/1‑93 (MQ=255)
                                                         gACTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCa                               <  2:2669333/93‑1 (MQ=255)
                                                         gACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCa                               >  2:6571196/1‑93 (MQ=255)
                                                          aCTATAACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCAc                              <  1:1055083/93‑1 (MQ=255)
                                                            tataACGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACgc                            <  2:5683465/93‑1 (MQ=255)
                                                             ataACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACgct                           >  2:6627740/1‑93 (MQ=255)
                                                               aaCGACGCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACgctgc                         >  2:4859341/1‑93 (MQ=255)
                                                                acgacgCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACgctgct                        <  1:2613618/93‑1 (MQ=255)
                                                                acgacgCACATGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACgctgct                        <  1:6600272/93‑1 (MQ=255)
                                                                 cgacgCACCTGCTGATGCTCAGCAGGCCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGctgctg                       <  1:5357156/93‑1 (MQ=255)
                                                                  gacgCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTgg                      >  1:3642596/1‑93 (MQ=255)
                                                                  gacgCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTgg                      >  1:2041056/1‑93 (MQ=255)
                                                                    cgCACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCa                    <  2:1807380/93‑1 (MQ=255)
                                                                      cACCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACt                  <  2:2921176/93‑1 (MQ=255)
                                                                      cACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAATGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACt                  >  1:6130070/1‑93 (MQ=255)
                                                                       aCCTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTa                 <  2:1678230/93‑1 (MQ=255)
                                                                        ccTGCTGATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTac                <  2:6588299/93‑1 (MQ=255)
                                                                          tgctgATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTacac              >  1:6637659/1‑93 (MQ=255)
                                                                           gctgATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACAcc             >  2:6079715/1‑93 (MQ=255)
                                                                            ctgATGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACAccc            <  1:6022458/93‑1 (MQ=255)
                                                                             tgATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCa           >  1:1521028/1‑93 (MQ=255)
                                                                                tGCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGa        <  2:2376585/93‑1 (MQ=255)
                                                                                tGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGa        <  1:3939614/93‑1 (MQ=255)
                                                                                 gCTCAGCAGGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGAt       <  2:5565378/93‑1 (MQ=255)
                                                                                    cagcagGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTc    <  2:5935226/93‑1 (MQ=255)
                                                                                    cagcagGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTc    <  2:1191909/93‑1 (MQ=255)
                                                                                     agcagGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCg   >  1:3797282/1‑93 (MQ=255)
                                                                                      gcagGGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCgg  <  1:4002384/93‑1 (MQ=255)
                                                                                          |                                                                                        
ACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGG  >  NC_000913/24947‑25125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑