BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,014,440	C→T	P103S (CCT→TCT)	ymbA →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,014,440	0	C	T	80.9%	592.3 / 117.8	246	P103S (CCT→TCT)	ymbA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (14/33); new base T (89/110); total (103/143)
Fisher's exact test for biased strand distribution p-value = 7.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01

Only 100 of 253 total aligned reads displayed.

TTGTGGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGG  >  NC_000913/1014350‑1014529

ttGTGGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTct                                                                                         <  2:1327554/93‑1 (MQ=255)

ttGTGGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTct                                                                                         >  2:1746350/1‑93 (MQ=255)

 tgtgGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGtctc                                                                                        <  2:3542646/93‑1 (MQ=255)

 tgtgGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGtctc                                                                                        <  2:6186645/93‑1 (MQ=255)

 tgtgGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCctc                                                                                        >  1:2033810/1‑93 (MQ=255)

  gtgGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCctct                                                                                       <  1:3746414/93‑1 (MQ=255)

   tgGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTCCCCGGCTGGGTGGTTGCCTCCCAGTCTCTg                                                                                      >  2:1530850/1‑93 (MQ=255)

    gggCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTgg                                                                                     >  1:3176599/1‑93 (MQ=255)

     ggCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTggg                                                                                    >  1:6499157/1‑93 (MQ=255)

     ggCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTggg                                                                                    >  2:2824920/1‑93 (MQ=255)

      gCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGa                                                                                   <  1:4780756/93‑1 (MQ=255)

       ccAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGaa                                                                                  <  1:6242952/93‑1 (MQ=255)

        cAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAg                                                                                 >  1:1603137/1‑93 (MQ=255)

        cAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGCGGTTCCCTCCCAGTCTCTGGGacg                                                                                 >  1:4301357/1‑91 (MQ=255)

         aGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAgc                                                                                <  2:1126510/93‑1 (MQ=255)

         aGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAgc                                                                                >  2:12203/1‑93 (MQ=255)

         aGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAgc                                                                                <  2:3207213/93‑1 (MQ=255)

          gCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAgcg                                                                               >  2:1620651/1‑93 (MQ=255)

          gCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAgcg                                                                               <  2:4023272/93‑1 (MQ=255)

           cccGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCCACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAgcgc                                                                              >  2:2508266/1‑93 (MQ=255)

           cccGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAgcgc                                                                              <  1:4754375/93‑1 (MQ=255)

             cGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGccc                                                                            <  1:3829068/93‑1 (MQ=255)

              gTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCa                                                                           <  1:5899917/93‑1 (MQ=255)

              gTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCa                                                                           <  1:1906296/93‑1 (MQ=255)

               ttGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAg                                                                          >  2:4248597/1‑93 (MQ=255)

                tGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAgg                                                                         <  1:89684/93‑1 (MQ=255)

                   aTCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGaca                                                                      <  2:2288208/93‑1 (MQ=255)

                   aTCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGaca                                                                      <  2:4328972/93‑1 (MQ=255)

                    tCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGacac                                                                     <  2:5583875/93‑1 (MQ=255)

                    tCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGacac                                                                     <  2:3364795/93‑1 (MQ=255)

                     cAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACg                                                                    >  2:3907318/1‑93 (MQ=255)

                     cAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACg                                                                    <  1:5695198/93‑1 (MQ=255)

                     cAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACg                                                                    >  2:5526166/1‑93 (MQ=255)

                       aCAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCt                                                                  >  2:6115475/1‑93 (MQ=255)

                         aGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCa                                                                >  2:2456427/1‑93 (MQ=255)

                          gTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCaa                                                               >  2:6617287/1‑93 (MQ=255)

                          gTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCaa                                                               <  1:5984422/93‑1 (MQ=255)

                           ttGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAAt                                                              >  2:1275098/1‑93 (MQ=255)

                           ttGCCCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAAt                                                              <  1:2999938/93‑1 (MQ=255)

                                 aaCACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTAcc                                                        <  2:256696/93‑1 (MQ=255)

                                 aaCACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTAcc                                                        <  2:5441011/93‑1 (MQ=255)

                                  acacCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCg                                                       >  2:1052317/1‑93 (MQ=255)

                                  acacCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCg                                                       >  1:1760484/1‑93 (MQ=255)

                                  acacCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCg                                                       >  2:4481856/1‑93 (MQ=255)

                                   cacCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGt                                                      <  2:4542678/93‑1 (MQ=255)

                                      ccTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAAc                                                   >  2:515537/1‑93 (MQ=255)

                                      ccTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAAc                                                   >  2:4689542/1‑93 (MQ=255)

                                       cTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACg                                                  >  2:6340484/1‑93 (MQ=255)

                                        tGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACgg                                                 <  1:3572583/93‑1 (MQ=255)

                                        tGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACgg                                                 <  2:6442722/93‑1 (MQ=255)

                                        tGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACgg                                                 <  2:1970076/93‑1 (MQ=255)

                                         ggTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCGATGTTACAGTAACGGa                                                >  1:4276509/1‑93 (MQ=255)

                                         ggTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGa                                                >  2:525072/1‑93 (MQ=255)

                                            tGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGtt                                             <  1:2521092/93‑1 (MQ=255)

                                            tGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGtt                                             <  1:3146844/93‑1 (MQ=255)

                                             gCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGttt                                            >  1:5804493/1‑93 (MQ=255)

                                               cAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTaa                                          <  1:5650849/93‑1 (MQ=255)

                                               cAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTaa                                          <  1:2705500/93‑1 (MQ=255)

                                               cAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTaa                                          <  1:22400/93‑1 (MQ=255)

                                                aaCCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAAc                                         >  2:4848511/1‑93 (MQ=255)

                                                 aCCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACg                                        <  1:5827643/93‑1 (MQ=255)

                                                  ccTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACgg                                       <  2:5621989/93‑1 (MQ=255)

                                                   cTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGt                                      <  1:3386192/93‑1 (MQ=255)

                                                    tGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTc                                     >  2:4231259/1‑93 (MQ=255)

                                                    tGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTc                                     >  1:5135922/1‑93 (MQ=255)

                                                    tGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTc                                     <  2:5931429/93‑1 (MQ=255)

                                                    tGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTc                                     <  1:6602212/93‑1 (MQ=255)

                                                      aGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGc                                   >  1:529901/1‑93 (MQ=255)

                                                       gTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCt                                  >  1:3719557/1‑93 (MQ=255)

                                                         aCGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTat                                <  1:5080480/93‑1 (MQ=255)

                                                         aCGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTat                                <  2:475685/93‑1 (MQ=255)

                                                         aCGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTat                                <  2:5957259/93‑1 (MQ=255)

                                                           gCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTatga                              >  2:2116085/1‑93 (MQ=255)

                                                           gCAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTatga                              <  2:3786148/93‑1 (MQ=255)

                                                            cAACTGCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTatgat                             <  1:4990381/93‑1 (MQ=255)

                                                                 gCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCaa                        >  1:2682372/1‑93 (MQ=255)

                                                                 gCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCaa                        >  1:5860785/1‑93 (MQ=255)

                                                                 gCCCGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCaa                        <  1:5543919/93‑1 (MQ=255)

                                                                    cGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGt                     <  2:827322/93‑1 (MQ=255)

                                                                    cGGCTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGt                     <  1:6077774/93‑1 (MQ=255)

                                                                       cTGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCAt                  <  1:4858577/93‑1 (MQ=255)

                                                                        tGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCAtt                 <  2:4605833/93‑1 (MQ=255)

                                                                        tGGGTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCAtt                 >  1:5272473/1‑93 (MQ=255)

                                                                         gggTGGTTGCCTCCCAGTCTCTGGGAAGCGCCCAGGAAACGCTCAATGTTAACGTAACGAAGCTTAACGGTAGCTATGATTGCAAGGTGATTg                >  1:4705071/1‑93 (MQ=255)

                                                                          ggtggtTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGt               >  2:3041420/1‑93 (MQ=255)

                                                                           gtggtTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTc              <  2:1667382/93‑1 (MQ=255)

                                                                            tggtTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCa             <  1:1043510/93‑1 (MQ=255)

                                                                            tgcttGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCa             <  1:3886824/90‑1 (MQ=255)

                                                                             ggtTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAg            >  1:5480935/1‑93 (MQ=255)

                                                                              gtTGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAgt           >  1:4950896/1‑93 (MQ=255)

                                                                               ttGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAgtg          <  1:3461653/93‑1 (MQ=255)

                                                                                tGCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAgtgg         <  1:2480115/93‑1 (MQ=255)

                                                                                 gCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAgtggt        <  1:1415433/93‑1 (MQ=255)

                                                                                 gCCTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAgtggt        <  2:73940/93‑1 (MQ=255)

                                                                                  ccTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAgtggtg       <  2:1578619/93‑1 (MQ=255)

                                                                                  ccTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAgtggtg       <  2:3622863/93‑1 (MQ=255)

                                                                                   cTCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGa      <  1:4621969/93‑1 (MQ=255)

                                                                                    tCCCAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAg     >  1:677307/1‑93 (MQ=255)

                                                                                     cccAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGt    <  1:2318252/93‑1 (MQ=255)

                                                                                       cAGTCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTgg  >  1:2858543/1‑93 (MQ=255)

TTGTGGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGG  >  NC_000913/1014350‑1014529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`