BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,747,389	Δ1 bp	coding (572/591 nt)	b4540 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,747,388	0	C	.	99.8%	2852.5 / ‑4.1	593	coding (571/591 nt)	b4540	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (283/309); minor base G (1/0); total (284/309)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 650 total aligned reads displayed.

GCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTGCGCC  >  NC_000913/2747293‑2747479

gCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCATTCAGGATATGCTGCTGTCGGTGCTCGATc                                                                                             <  1:407637/96‑1 (MQ=255)

gCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATc                                                                                             <  1:4548988/96‑1 (MQ=255)

 ctctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATct                                                                                            <  2:862859/96‑2 (MQ=255)

  tctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATctg                                                                                           >  1:8032926/1‑94 (MQ=255)

  tctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATctg                                                                                           >  2:919977/1‑94 (MQ=255)

  tctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATctg                                                                                           >  1:13971231/1‑94 (MQ=255)

    tttGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATctgga                                                                                         >  2:5754284/1‑92 (MQ=255)

     ttGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaa                                                                                       <  1:272340/96‑1 (MQ=255)

      tGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaa                                                                                      >  2:744938/1‑96 (MQ=255)

        aGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaa                                                                                    >  2:9758280/1‑96 (MQ=255)

         gCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaaa                                                                                   >  1:5628309/1‑96 (MQ=255)

         gCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaaa                                                                                   >  2:1654026/1‑96 (MQ=255)

           aaaGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATg                                                                                 >  2:1314253/1‑96 (MQ=255)

            aagaagAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGa                                                                                <  2:13794441/96‑1 (MQ=255)

             agaagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGAc                                                                               >  2:7742194/1‑96 (MQ=255)

                agagTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGGGCTCGAT‑CTGGAAAAAATGACCGt                                                                            >  1:6867226/1‑96 (MQ=255)

                  agTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtg                                                                          <  2:11172410/96‑1 (MQ=255)

                    ttGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtgat                                                                        <  1:7415918/96‑1 (MQ=255)

                     tGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtgatg                                                                       <  2:6986903/96‑1 (MQ=255)

                      gcgcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtgatga                                                                      <  2:9838901/96‑1 (MQ=255)

                       cgcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtgat                                                                        >  1:2050497/1‑93 (MQ=255)

                        gcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAca                                                                    >  2:427233/1‑96 (MQ=255)

                        gcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAca                                                                    <  2:1327469/96‑1 (MQ=255)

                        gcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAca                                                                    >  1:12301571/1‑96 (MQ=255)

                        gcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAca                                                                    <  1:539014/96‑1 (MQ=255)

                        gcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAca                                                                    >  2:9108637/1‑96 (MQ=255)

                         cACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcat                                                                   >  1:525098/1‑96 (MQ=255)

                          aCTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGGT‑CTGGAAAAAATGACCGTTGATGAcatc                                                                  >  1:13715785/1‑96 (MQ=255)

                          aCTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatc                                                                  <  2:11730957/96‑1 (MQ=255)

                           cTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatca                                                                 >  1:598664/1‑96 (MQ=255)

                           cTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatca                                                                 <  1:8658861/96‑1 (MQ=255)

                           cTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatca                                                                 >  2:12923373/1‑96 (MQ=255)

                           cTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATACTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatca                                                                 <  1:8244339/96‑1 (MQ=255)

                            tATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatcat                                                                <  2:6646204/96‑1 (MQ=255)

                            tATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatcat                                                                <  1:7613437/96‑1 (MQ=255)

                             aTCGTGCACGAATCACGCTCACAAATTTCGCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGAACGTTGATGACATCATg                                                               <  1:12110448/96‑1 (MQ=255)

                             aTCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATg                                                               >  2:215184/1‑96 (MQ=255)

                             aTCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATg                                                               <  2:8255000/96‑1 (MQ=255)

                              tCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatcattg                                                              <  1:8113517/96‑3 (MQ=255)

                              tCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATgg                                                              <  1:7133865/96‑1 (MQ=255)

                               cGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGt                                                             <  2:9522382/96‑1 (MQ=255)

                                gTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTg                                                            <  1:10549863/96‑1 (MQ=255)

                                  gCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTg                                                            >  2:4022677/1‑94 (MQ=255)

                                  gCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGcc                                                          <  2:2515103/96‑1 (MQ=255)

                                  gCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGcc                                                          <  2:4887726/96‑1 (MQ=255)

                                    aCGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcgc                                                        >  2:6020486/1‑96 (MQ=255)

                                     cGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcgcg                                                       >  2:11075549/1‑96 (MQ=255)

                                      gAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCgcgc                                                      >  1:9778399/1‑96 (MQ=255)

                                      gAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCgcgc                                                      >  2:10586979/1‑96 (MQ=255)

                                       aaTCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCa                                                     >  2:3543688/1‑96 (MQ=255)

                                       aaTCACGCTCACAAATTTCCAGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCa                                                     >  2:5434081/1‑96 (MQ=255)

                                        aTCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAg                                                    >  2:8915760/1‑96 (MQ=255)

                                         tCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCaat                                                   <  1:14280635/96‑3 (MQ=255)

                                           aCGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGa                                                 >  2:8008576/1‑96 (MQ=255)

                                           aCGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGGCGGTGCTCGA‑GCTGGAAAAAATGAGCGGTTATGACATCATGGGGCCGCGCAGGGa                                                 >  1:9260103/1‑96 (MQ=255)

                                           aCGCTCACAAATTTCCCGTCGCAATCAGGATATGCTCCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGa                                                 >  2:5222313/1‑96 (MQ=255)

                                               tgacaAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAAtt                                             >  1:11042840/3‑96 (MQ=255)

                                               tCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAAtt                                             <  2:14394514/96‑1 (MQ=255)

                                               tCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGGTGATGACATCATTGTGCCGCGCAGAGAAAtt                                             >  1:3296141/1‑96 (MQ=255)

                                                 acaAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTAt                                           >  1:4360251/1‑96 (MQ=255)

                                                   aaaTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCg                                         >  2:10685613/1‑96 (MQ=255)

                                                   aaaTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCg                                         <  1:14120299/96‑1 (MQ=255)

                                                   aaaTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCg                                         >  2:2791620/1‑96 (MQ=255)

                                                      tttCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTa                                      <  1:10044674/96‑1 (MQ=255)

                                                       ttCCCGTCGCAATCAGGATATGCTGCTGTCGGTTCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAt                                     <  1:9528925/96‑1 (MQ=255)

                                                       ttCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAt                                     >  2:11526390/1‑96 (MQ=255)

                                                         cccGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTg                                   >  2:7336310/1‑96 (MQ=255)

                                                           cGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGat                                 >  1:6494866/1‑96 (MQ=255)

                                                              cGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATc                              <  2:4624302/96‑1 (MQ=255)

                                                                 gaTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAAc                           >  1:11268767/2‑96 (MQ=255)

                                                                  aTCAGGATATGCTGCTGTCGGTGCTTGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATTGGTCTTGATATCAACg                          >  1:3561570/1‑96 (MQ=255)

                                                                  aTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACg                          <  1:8509424/96‑1 (MQ=255)

                                                                    cAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                        >  1:12629678/1‑96 (MQ=255)

                                                                    cAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                        >  1:3683284/1‑96 (MQ=255)

                                                                    cAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                        >  1:2318700/1‑96 (MQ=255)

                                                                    cAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                        <  2:6236624/96‑1 (MQ=255)

                                                                    cAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCATTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                        >  1:11594223/1‑96 (MQ=255)

                                                                     aGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATg                       >  1:3171770/1‑96 (MQ=255)

                                                                     aGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGGATTGATATCAACGATg                       >  2:13620447/1‑96 (MQ=255)

                                                                      ggATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGa                      <  2:3679997/96‑1 (MQ=255)

                                                                       gATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGAc                     >  1:13298537/1‑96 (MQ=255)

                                                                       gATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGAc                     >  2:1930745/1‑96 (MQ=255)

                                                                         tatGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTg                   <  1:488239/96‑1 (MQ=255)

                                                                           tgctgcTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGa                 <  2:14165438/96‑1 (MQ=255)

                                                                             ctgctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGCGCCGCGCAGTGAAATTATCGGGATTGATATCAACGATGACTGGaaa               >  2:12016773/1‑96 (MQ=255)

                                                                              tgctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAAt              <  2:1229374/96‑1 (MQ=255)

                                                                              tgctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACGGGAAAt              >  1:6268664/1‑96 (MQ=255)

                                                                               gctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATc             <  1:1938860/96‑1 (MQ=255)

                                                                               gctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATc             >  2:10275911/1‑96 (MQ=255)

                                                                               gcggTCGGGGCTCGAT‑CGGGAAAAAATGACCGTTGATGACATCATGGGGCCGCGCAGTGAAATTATCGTAATTGATGTCAACGATGACTGGAAATc             >  1:8951974/4‑96 (MQ=255)

                                                                                ctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCg            <  1:7862469/96‑1 (MQ=255)

                                                                                ctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTACCGCGCAGTGAAATTATCGGTATTGATACCAACGATGACTGGAAATCg            <  2:12753967/96‑1 (MQ=255)

                                                                                 tgTCGGTGCTCGAT‑CTGGAAAAAATAACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGa           >  1:13537123/1‑96 (MQ=255)

                                                                                  gTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGAt          >  2:3137544/1‑96 (MQ=255)

                                                                                   tCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGAtt         <  2:3930613/96‑1 (MQ=255)

                                                                                    cGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAAGCGATTc        >  1:1538050/1‑96 (MQ=255)

                                                                                    cGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGGATTGATATCAACGATGACTGGAAATCGATTc        >  2:3338889/1‑96 (MQ=255)

                                                                                     ggTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCt       <  1:7336594/96‑1 (MQ=255)

                                                                                     ggTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCt       <  2:14019394/96‑1 (MQ=255)

                                                                                        gCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTgcg    <  1:13016578/96‑1 (MQ=255)

GCTCTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTGCGCC  >  NC_000913/2747293‑2747479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`