Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,998,290 +G coding (379/486 nt) b4566 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,998,2891.G99.8% 1918.4 / NA 488L127P (CTG→CCG) b4566hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (246/241);  minor base T (0/1);  total (246/242)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 545 total aligned reads displayed.

CCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCA  >  NC_000913/3998194‑3998384
                                                                                                |                                                                                               
 cTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                                >  2:3423853/1‑96 (MQ=255)
 cTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                                >  2:10391508/1‑96 (MQ=255)
 cTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                                >  1:9790015/1‑96 (MQ=255)
  tGACTGCACCGCAATCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gt                                                                                               <  1:11321190/96‑2 (MQ=255)
  tGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gg                                                                                               <  2:5467901/96‑2 (MQ=255)
    aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggac                                                                                             >  2:6452235/1‑93 (MQ=255)
    aCTGCACCGCAAACTGAATCCCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACCCGCCGCCA‑ggac                                                                                             <  2:1902236/96‑4 (MQ=255)
        cACCGCAAACTGAGTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                          >  2:7431181/1‑96 (MQ=255)
        cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                          <  1:8531642/96‑1 (MQ=255)
        cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                          <  2:46563/96‑1 (MQ=255)
          ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaa                                                                                        >  1:4747873/1‑96 (MQ=255)
           cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                                       >  1:9057286/1‑96 (MQ=255)
           cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                                       <  2:13741401/96‑1 (MQ=255)
             cAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGa                                                                                     <  1:4973376/96‑1 (MQ=255)
             cAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGa                                                                                     <  2:10685327/96‑1 (MQ=255)
                aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCTGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAt                                                                                  >  2:12215138/1‑96 (MQ=255)
                aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAt                                                                                  <  2:11350468/96‑1 (MQ=255)
                   gAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTcc                                                                               <  1:30906/96‑1 (MQ=255)
                    aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAAAAATTccc                                                                              >  1:6419283/1‑96 (MQ=255)
                    aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACACAAAACACGCCGCCAGGACGGATGAAGAAATTgcc                                                                              >  1:13978729/1‑96 (MQ=255)
                         ctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAc                                                                         <  2:1530636/96‑1 (MQ=255)
                          tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAcc                                                                        <  1:6117960/96‑1 (MQ=255)
                          tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTCCAcc                                                                        >  1:5761056/1‑96 (MQ=255)
                           ttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCt                                                                       <  2:277696/96‑1 (MQ=255)
                            tACTGGATTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTg                                                                      >  1:4054874/1‑96 (MQ=255)
                             aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGa                                                                     <  1:1435712/96‑1 (MQ=255)
                               tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAg                                                                   <  2:6132700/96‑1 (MQ=255)
                                ggTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGc                                                                  <  2:8005302/96‑1 (MQ=255)
                                  ttttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGcta                                                                <  1:11747646/96‑3 (MQ=255)
                                   tttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgag                                                               <  1:12357374/96‑1 (MQ=255)
                                   tttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgag                                                               <  1:2946382/96‑1 (MQ=255)
                                   gttGAAGGCATCCAGACGACCGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgag                                                               <  1:3418429/95‑1 (MQ=255)
                                      gAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAtt                                                            >  2:9143057/1‑96 (MQ=255)
                                       aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAttt                                                           >  2:4158819/1‑96 (MQ=255)
                                       aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAttt                                                           <  2:12811307/96‑1 (MQ=255)
                                       aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAttt                                                           >  2:9449366/1‑96 (MQ=255)
                                        aGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTa                                                          <  2:14237509/96‑1 (MQ=255)
                                         ggCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTaa                                                         >  2:10939667/1‑96 (MQ=255)
                                           cATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACg                                                       >  1:7271275/1‑96 (MQ=255)
                                           cATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACg                                                       >  1:9383171/1‑96 (MQ=255)
                                            aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGc                                                      <  1:43538/96‑1 (MQ=255)
                                            aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGc                                                      >  1:13738577/1‑96 (MQ=255)
                                             tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGcc                                                     >  2:7236931/1‑96 (MQ=255)
                                              ccAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                                    <  2:8409030/96‑1 (MQ=255)
                                                aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAcc                                                  >  1:1357418/1‑96 (MQ=255)
                                                 gACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCt                                                 <  1:1577017/96‑1 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                              >  2:3645432/1‑96 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                              >  2:5722856/1‑96 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                              <  2:2445726/96‑1 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAGTCCCTCCACCTGAAGCGAGATTTAACGACACCTTct                                              <  2:12983005/96‑1 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACAGAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCctct                                              >  1:12066681/1‑96 (MQ=255)
                                                     gaggaTGCCGTCTTTATCTACCGCGACATAAACCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctg                                             <  1:14138972/93‑1 (MQ=255)
                                                      acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                            <  2:7836591/96‑1 (MQ=255)
                                                      acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                            >  1:7817544/1‑96 (MQ=255)
                                                       cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgct                                           >  2:11845539/1‑96 (MQ=255)
                                                       cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgct                                           >  1:1906103/1‑96 (MQ=255)
                                                       cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgct                                           >  1:2114879/1‑96 (MQ=255)
                                                        gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgctg                                          >  1:760871/1‑96 (MQ=255)
                                                          tGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAc                                        >  2:12441525/1‑96 (MQ=255)
                                                          tGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAc                                        >  1:3669022/1‑96 (MQ=255)
                                                           gCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                                       <  1:2782700/96‑1 (MQ=255)
                                                           gCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                                       <  2:216942/96‑1 (MQ=255)
                                                           gCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                                       >  1:12984705/1‑96 (MQ=255)
                                                            ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                                      <  2:8918891/96‑1 (MQ=255)
                                                            ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                                      >  1:11622599/1‑96 (MQ=255)
                                                            ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                                      <  2:14089047/96‑1 (MQ=255)
                                                             cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGt                                     <  1:9482817/96‑1 (MQ=255)
                                                             cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGt                                     >  2:13274069/1‑96 (MQ=255)
                                                             cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGt                                     <  1:12789750/96‑1 (MQ=255)
                                                               aCTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGttt                                   <  2:4462245/96‑1 (MQ=255)
                                                                cTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                                  <  2:1788329/96‑1 (MQ=255)
                                                                cTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                                  <  2:13536578/96‑1 (MQ=255)
                                                                 tttATCTCCCGCGACATAAGACACGCCGACAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCTCCTTCTGCTGACCGTTTTc                                 >  1:83564/1‑96 (MQ=255)
                                                                  ttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCg                                >  2:5926350/1‑96 (MQ=255)
                                                                    aTCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAt                              <  2:11025260/96‑1 (MQ=255)
                                                                     tctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATg                             >  2:2995234/1‑96 (MQ=255)
                                                                        cccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                          >  1:3890752/1‑96 (MQ=255)
                                                                        cccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                          <  1:6782595/96‑1 (MQ=255)
                                                                         ccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACa                         <  1:9986507/96‑1 (MQ=255)
                                                                           gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaaa                       <  2:11477374/96‑1 (MQ=255)
                                                                            cgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc                      >  2:9719909/1‑96 (MQ=255)
                                                                             gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc                     >  1:7990829/1‑96 (MQ=255)
                                                                             gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc                     <  1:7397495/96‑1 (MQ=255)
                                                                               cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCCCCTTCTGCTGACCGTTTTCGATGTACAAACCGa                   <  2:385577/96‑1 (MQ=255)
                                                                               cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGa                   <  1:8683066/96‑1 (MQ=255)
                                                                                aTAAACCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAg                  <  1:5174475/96‑1 (MQ=255)
                                                                                aTAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAg                  >  2:108244/1‑96 (MQ=255)
                                                                                 tAAAACACGCCGCCAGGACGGATCAAGAAATTCCCTTCACCTGAAGCGAGATTTAACCCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgc                 <  2:12613817/96‑1 (MQ=255)
                                                                                 tAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgc                 >  1:13362755/1‑96 (MQ=255)
                                                                                 tAAAACACGCCGCCAGGACGGATAAAGAAAGTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGacc                 <  1:10283295/96‑3 (MQ=255)
                                                                                   aaaCACGCCGCCAGGACGGATAAAGAAATTCCATTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcgg               <  1:5419966/96‑1 (MQ=255)
                                                                                    aaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc              <  1:7169673/96‑1 (MQ=255)
                                                                                      ctcgccgcCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGgcgc            >  1:11046944/3‑96 (MQ=255)
                                                                                       acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGTGCa           >  1:2363537/1‑96 (MQ=255)
                                                                                       acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa           >  1:10017942/1‑96 (MQ=255)
                                                                                       acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa           <  2:12087058/96‑1 (MQ=255)
                                                                                       acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa           >  2:13953317/1‑96 (MQ=255)
                                                                                        cgccgcCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGgcgcct          <  1:10730084/96‑3 (MQ=255)
                                                                                           cgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGc       >  2:8645421/1‑96 (MQ=255)
                                                                                               a‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtca  <  2:5256210/96‑1 (MQ=255)
                                                                                                |                                                                                               
CCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCA  >  NC_000913/3998194‑3998384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑