BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	981,566	C→T	R433C (CGC→TGC)	ycbB →	putative amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	981,566	0	C	T	99.7%	1047.7 / NA	295	R433C (CGC→TGC)	ycbB	putative amidase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (141/153); minor base G (1/0); total (142/153)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

GGTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCACGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAA  >  NC_000913/981476‑981655

ggtcgCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGc                                                                                         >  1:7040480/1‑93 (MQ=255)

 gtcgCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCa                                                                                        <  1:5350563/93‑1 (MQ=255)

  tcgCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCaa                                                                                       >  2:876400/1‑93 (MQ=255)

      cccGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGat                                                                                   >  1:1293212/1‑93 (MQ=255)

       ccGATCGCAAAACGCCGATGATGAGCAGTGGCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGata                                                                                  >  1:2691545/1‑93 (MQ=255)

       ccGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGata                                                                                  <  1:4286981/93‑1 (MQ=255)

       ccGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGata                                                                                  >  1:670958/1‑93 (MQ=255)

       ccGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGata                                                                                  >  1:6646426/1‑93 (MQ=255)

        cGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGatat                                                                                 <  2:604284/93‑1 (MQ=255)

         gATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATAtt                                                                                >  2:6543928/1‑93 (MQ=255)

          aTCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGCCATGCAAAGATATTc                                                                               >  1:4538704/1‑93 (MQ=255)

          aTCGCAAAACGCCGATGATGAGCAGGGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTCGCATGCAAAGatatac                                                                               >  2:1575262/1‑91 (MQ=255)

            cGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTCCCTCCAACTCTGGCATGCAAAGATATTCTg                                                                             <  2:6714399/93‑1 (MQ=255)

             gCAAAACGCCGATGATTAGCAGTGCCCTTAACAACGTAGTGGTAAT‑CCCTTCGTGGAACGTACCTCCAACTCTGGCATGCTAAGATAATCTGc                                                                            >  2:7836448/1‑93 (MQ=255)

               aaaaCGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCa                                                                          >  1:41207/1‑93 (MQ=255)

                aaaCGCCGATGATGAGCAGTGCCCTTAACAAGGTAGGGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaa                                                                         <  1:566964/93‑1 (MQ=255)

                aaaCGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaa                                                                         >  2:6265299/1‑93 (MQ=255)

                aaaCGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaa                                                                         >  1:7440692/1‑93 (MQ=255)

                  aCGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaaaa                                                                       >  1:8777346/1‑93 (MQ=255)

                  aCGCCGATGAGGGAGAGTGCCCTTAACAACGTAGTGGTAAACCCCGCCG‑GGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCaaaa                                                                       <  1:5755052/93‑1 (MQ=255)

                   cGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAg                                                                      <  2:7952243/93‑1 (MQ=255)

                    gCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGt                                                                     <  1:8212275/93‑1 (MQ=255)

                    gCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGt                                                                     >  1:5202091/1‑93 (MQ=255)

                    gCCGATAATGGGCAGTGCCCTAAACAACGAAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGt                                                                     <  2:7008686/93‑1 (MQ=255)

                      cGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTgc                                                                   >  1:2250415/1‑93 (MQ=255)

                        atgatgAGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTgcgc                                                                 <  2:260704/93‑1 (MQ=255)

                        atgatgAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTgcgc                                                                 <  2:4965911/93‑1 (MQ=255)

                          gatgaGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCaa                                                               >  2:2729172/1‑93 (MQ=255)

                           atgaGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAAc                                                              <  1:5885964/93‑1 (MQ=255)

                             gaGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGa                                                            >  1:537266/1‑93 (MQ=255)

                             gaGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGa                                                            >  1:1859746/1‑93 (MQ=255)

                             gaGCAGTCCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGa                                                            <  2:4015705/93‑1 (MQ=255)

                              atcggTGCCCTTAACAACGTAGTGGAAAG‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGAt                                                           <  1:7595343/89‑1 (MQ=255)

                              aGCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGAt                                                           <  1:6844837/93‑1 (MQ=255)

                               gCAGTGCCCTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATc                                                          <  2:1909708/93‑1 (MQ=255)

                               gCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATc                                                          <  2:7036442/93‑1 (MQ=255)

                                cggTGCCCTTAACATCTTGGTGGAAAC‑CCCGCCGTGGAACGTACCTCCAACCCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATcc                                                         <  2:4779264/91‑1 (MQ=255)

                                 aGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATACg                                                        <  1:8365424/93‑1 (MQ=255)

                                  gTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCgg                                                       <  1:4346193/93‑1 (MQ=255)

                                  gTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCgg                                                       >  1:1680689/1‑93 (MQ=255)

                                   tGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCggg                                                      <  1:4470952/93‑1 (MQ=255)

                                    ccccTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGa                                                     <  2:2143006/92‑1 (MQ=255)

                                     cccTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGat                                                    >  2:2306753/1‑93 (MQ=255)

                                     cccTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGat                                                    <  2:1320430/93‑1 (MQ=255)

                                       cTTAACAACGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGatat                                                  <  2:4318734/93‑1 (MQ=255)

                                        ttAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATAtc                                                 >  1:2367097/1‑93 (MQ=255)

                                        ttAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATAtc                                                 <  2:7358100/93‑1 (MQ=255)

                                         tAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATAtct                                                <  1:3848832/93‑1 (MQ=255)

                                           acaccGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCg                                              <  2:5070571/93‑1 (MQ=255)

                                           acaacGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCg                                              <  1:714984/93‑1 (MQ=255)

                                             agcGTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGaa                                            <  2:6843800/91‑1 (MQ=255)

                                             aacTTAGTGGTAAC‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGaa                                            <  2:412698/93‑1 (MQ=255)

                                              acGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGaaa                                           >  1:2580781/1‑93 (MQ=255)

                                                gTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGc                                         >  2:1280742/1‑93 (MQ=255)

                                                gTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGc                                         <  1:3234335/93‑1 (MQ=255)

                                                gTAGTGGAAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGc                                         <  1:7551149/93‑1 (MQ=255)

                                                 tAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGcc                                        >  1:2112854/1‑93 (MQ=255)

                                                 tAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGcc                                        <  2:8709635/93‑1 (MQ=255)

                                                  aGTGGTAAA‑CCCGCCGTGGAACGTGCCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCa                                       <  1:7780253/93‑1 (MQ=255)

                                                  aGTGGTAAA‑CCCGCCGTGGAACGTACCTGCAACTCTGGCATGCAAAGATATTCTGCCAAAAGGGCGCAACGATCCGGGATATCTCGAAAGCCa                                       >  1:858627/1‑93 (MQ=255)

                                                  aGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGcaa                                       >  1:2853413/1‑91 (MQ=255)

                                                   gTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCAt                                      >  1:7883100/1‑93 (MQ=255)

                                                     ggTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATgg                                    >  2:7574996/1‑93 (MQ=255)

                                                     ggTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATgg                                    <  2:3637455/93‑1 (MQ=255)

                                                      gTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGc                                   >  1:1071227/1‑93 (MQ=255)

                                                      gTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGc                                   <  2:7102692/93‑1 (MQ=255)

                                                       gaaa‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCt                                  <  1:1822716/92‑1 (MQ=255)

                                                         aa‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCtat                                >  1:5092763/1‑93 (MQ=255)

                                                          a‑CCCGCCGTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCtata                               <  1:6159783/93‑1 (MQ=255)

                                                               gccgTGGAGCGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATATCGAAAGCCATGGCTATACGGt                           <  2:8323803/93‑1 (MQ=255)

                                                               gccgTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGt                           >  2:6167981/1‑93 (MQ=255)

                                                                ccgTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTg                          >  2:4721605/1‑93 (MQ=255)

                                                                 cgTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTTCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGa                         <  1:7371958/93‑1 (MQ=255)

                                                                 cgTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGa                         <  1:978622/93‑1 (MQ=255)

                                                                 cgTGAAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGa                         <  1:119610/93‑1 (MQ=255)

                                                                  gTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAt                        >  1:472543/1‑93 (MQ=255)

                                                                  gTGGAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGAt                        >  2:3853380/1‑93 (MQ=255)

                                                                    ggAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgc                      <  2:5420300/93‑1 (MQ=255)

                                                                    ggAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgc                      <  2:3760926/93‑1 (MQ=255)

                                                                     gAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgcg                     >  2:4820368/1‑93 (MQ=255)

                                                                     gAACGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgcg                     >  2:1240756/1‑93 (MQ=255)

                                                                      aaCGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATgcgc                    >  2:240928/1‑93 (MQ=255)

                                                                       aCGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGcgcg                   >  2:2900724/1‑93 (MQ=255)

                                                                       aCGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGcgcg                   >  2:1127566/1‑93 (MQ=255)

                                                                        cGTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCgg                  >  2:8498959/1‑93 (MQ=255)

                                                                         gTACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGc                 <  2:3063501/93‑1 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                <  2:5833895/93‑1 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                >  1:4058093/1‑93 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                >  2:3124782/1‑93 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                >  2:6996336/1‑93 (MQ=255)

                                                                          tACCTCCAACTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCt                >  1:3059836/1‑93 (MQ=255)

                                                                                 aaCTCTGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcag         <  2:5299148/93‑1 (MQ=255)

                                                                                  aCTCTGGCATGCAAAGATATTCCGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcagc        >  2:7784294/1‑93 (MQ=255)

                                                                                   ctctGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcagca       <  1:6321701/93‑1 (MQ=255)

                                                                                   ctctGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcagca       <  2:4515610/93‑1 (MQ=255)

                                                                                    tctGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAAcagcag      >  2:5583480/1‑93 (MQ=255)

                                                                                     ctGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCaga     <  1:1468822/93‑1 (MQ=255)

                                                                                     caggCATGCAAAGATATTCTGCCAAAAGTCCGCATCGATCCGGGATATCACGAAAGCCATGGCTATACTGGGATGCGCGGCTGGAACAGCaga     <  2:7686420/91‑1 (MQ=255)

                                                                                      tGGCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCagag    >  1:65646/1‑93 (MQ=255)

                                                                                       ggCATGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAgaga   <  2:334319/93‑1 (MQ=255)

GGTCGCCCCGATCGCAAAACGCCGATGATGAGCAGTGCCCTTAACAACGTAGTGGTAAA‑CCCGCCGTGGAACGTACCTCCAACTCTGGCACGCAAAGATATTCTGCCAAAAGTGCGCAACGATCCGGGATATCTCGAAAGCCATGGCTATACGGTGATGCGCGGCTGGAACAGCAGAGAA  >  NC_000913/981476‑981655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`