Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,252,715 A→G S821P (TCC→CCC)  b1202 ← putative adhesion and penetration protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,252,7150AG99.1% 739.7 / NA 217S821P (TCC→CCC) b1202putative adhesion and penetration protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (119/96);  minor base T (1/1);  total (120/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

GGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGACAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTA  >  NC_000913/1252625‑1252804
                                                                                          |                                                                                         
ggCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCa                                                                                         <  2:3978318/93‑1 (MQ=255)
 gCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAg                                                                                        >  2:5236618/1‑93 (MQ=255)
  ccATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAgg                                                                                       >  2:1807968/1‑93 (MQ=255)
  ccATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAgg                                                                                       >  1:1088261/1‑93 (MQ=255)
   cATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGt                                                                                      <  2:3765766/93‑1 (MQ=255)
   cATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGt                                                                                      >  1:668739/1‑93 (MQ=255)
     ttACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTg                                                                                    >  1:3933720/1‑93 (MQ=255)
     ttACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTg                                                                                    >  2:7503344/1‑93 (MQ=255)
      tACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGa                                                                                   >  1:926507/1‑93 (MQ=255)
      tACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGa                                                                                   >  2:6893901/1‑93 (MQ=255)
           gCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGCTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACctc                                                                              <  1:3763634/93‑1 (MQ=255)
            ccTTCATAGTCATCTCATTGTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACctcg                                                                             >  1:2942909/1‑92 (MQ=255)
            ccTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACctct                                                                             <  2:1751067/93‑1 (MQ=255)
            ccTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACctct                                                                             >  2:5725361/1‑93 (MQ=255)
             cTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTg                                                                            >  1:7215196/1‑93 (MQ=255)
              ttCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGc                                                                           >  1:6613499/1‑93 (MQ=255)
              ttCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGc                                                                           <  2:1720600/93‑1 (MQ=255)
              ttCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGc                                                                           >  1:1286531/1‑93 (MQ=255)
               tCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGcc                                                                          >  1:5447605/1‑93 (MQ=255)
                cATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGccc                                                                         >  2:7667554/1‑93 (MQ=255)
                cATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGccc                                                                         >  2:2367750/1‑93 (MQ=255)
                 aTAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCg                                                                        <  2:5034751/93‑1 (MQ=255)
                  tAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCgg                                                                       >  1:5919086/1‑93 (MQ=255)
                   aGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGc                                                                      <  2:8526358/93‑1 (MQ=255)
                    gTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCt                                                                     <  2:8000639/93‑1 (MQ=255)
                    gTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCt                                                                     <  1:2655122/93‑1 (MQ=255)
                     tCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCtt                                                                    >  2:3750916/1‑93 (MQ=255)
                      cATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTc                                                                   >  1:2741407/1‑93 (MQ=255)
                       aTCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGATCCTCTGCCCGGCTTcc                                                                  <  1:1198509/93‑1 (MQ=255)
                       aTCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACGTCTGCCCGGCTTcc                                                                  <  2:8268668/93‑1 (MQ=255)
                       aTCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTcc                                                                  <  1:86708/93‑1 (MQ=255)
                        tctcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCa                                                                 <  1:2363300/93‑1 (MQ=255)
                         ctcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAg                                                                >  2:8396368/1‑93 (MQ=255)
                          tcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAgg                                                               <  1:6383209/93‑1 (MQ=255)
                          tcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAgg                                                               >  1:6352338/1‑93 (MQ=255)
                          tcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAgg                                                               >  2:7143024/1‑93 (MQ=255)
                          tcATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAgg                                                               >  1:8245963/1‑93 (MQ=255)
                              tCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGgagc                                                           >  2:6378935/1‑92 (MQ=255)
                              tCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGgaga                                                           <  2:6806039/93‑1 (MQ=255)
                              tCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGgaga                                                           >  1:4400054/1‑93 (MQ=255)
                               cTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGAt                                                          <  2:5654771/93‑1 (MQ=255)
                               cTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGAt                                                          >  1:210869/1‑93 (MQ=255)
                                 ggtggCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGc                                                        <  1:8715330/93‑1 (MQ=255)
                                    ggCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTca                                                     >  2:6638129/1‑93 (MQ=255)
                                    ggCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTca                                                     >  1:7810128/1‑93 (MQ=255)
                                     gCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcat                                                    >  2:4258393/1‑93 (MQ=255)
                                        gtatgtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattcc                                                 >  1:1816259/1‑93 (MQ=255)
                                        gtatgtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattcc                                                 >  1:1477107/1‑93 (MQ=255)
                                         tatgtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattcca                                                >  1:3557942/1‑93 (MQ=255)
                                          atgtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattccat                                               <  1:2792/93‑1 (MQ=255)
                                           tgtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattccatt                                              <  1:1557966/93‑1 (MQ=255)
                                           tgtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattccatt                                              <  1:4211413/93‑1 (MQ=255)
                                            gtaAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattccattc                                             <  1:2331756/93‑1 (MQ=255)
                                            gtaAGCTGGGTTTGCCGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTcattccattc                                             >  2:8710012/1‑93 (MQ=255)
                                               aGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCa                                          >  2:8172904/1‑93 (MQ=255)
                                               aGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCa                                          <  1:5716203/93‑1 (MQ=255)
                                                gCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAg                                         >  2:3104603/1‑93 (MQ=255)
                                                 cTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAgg                                        >  2:659410/1‑92 (MQ=255)
                                                 cTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGt                                        <  1:6040642/93‑1 (MQ=255)
                                                 cTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGt                                        >  1:7656501/1‑93 (MQ=255)
                                                  tGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTTCATTCGCAGTg                                       >  2:2168112/1‑93 (MQ=255)
                                                   gggTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGc                                      >  2:2119578/1‑93 (MQ=255)
                                                    ggTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGcc                                     <  2:5118776/93‑1 (MQ=255)
                                                      tttGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGt                                   <  2:4902842/93‑1 (MQ=255)
                                                       ttGCGGCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGtt                                  <  1:974797/93‑1 (MQ=255)
                                                            gCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGt                             >  1:1383501/1‑93 (MQ=255)
                                                            gCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGt                             >  1:4012943/1‑93 (MQ=255)
                                                            gCTCTATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGt                             >  2:1101791/1‑93 (MQ=255)
                                                             ctctATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGtt                            >  2:4685148/1‑93 (MQ=255)
                                                             ctctATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGtt                            >  2:2617378/1‑93 (MQ=255)
                                                              tctATATAGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTa                           >  1:492759/1‑93 (MQ=255)
                                                               ctATATAGAACCCATAACCAGTAGGGGGCAGGTTGACCCTCTGCCCGGCTGCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTaa                          <  1:6672120/93‑1 (MQ=255)
                                                                tatataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAAc                         >  1:6299532/1‑93 (MQ=255)
                                                                tatataGAACCCATAACCAGTAGGGGGCAGGCTGAACCTCTGCACGGCTTCCAGGGAGGTCCCGATTCCATTTGCACTGCGGCTGGCGGTAAc                         >  1:7112507/1‑93 (MQ=255)
                                                                  tataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGc                       <  2:4194252/93‑1 (MQ=255)
                                                                  tataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGc                       >  2:2768785/1‑93 (MQ=255)
                                                                  tataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGc                       >  1:5488701/1‑93 (MQ=255)
                                                                  tataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGc                       <  1:6844530/93‑1 (MQ=255)
                                                                   ataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                      >  1:2273762/1‑93 (MQ=255)
                                                                   ataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                      <  1:7757992/93‑1 (MQ=255)
                                                                   ataGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGcc                      >  2:1365414/1‑93 (MQ=255)
                                                                     aGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgt                    <  2:7391370/93‑1 (MQ=255)
                                                                     aGAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgt                    <  2:5874251/93‑1 (MQ=255)
                                                                      gaccccATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgtt                   <  2:3515054/90‑1 (MQ=255)
                                                                      gAACCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCGGGCTTCAAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTACGGCCgtg                   >  1:847706/1‑92 (MQ=255)
                                                                       aaCCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgttg                  <  1:8212982/93‑1 (MQ=255)
                                                                        aCCCATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCgttgt                 <  2:1381251/93‑1 (MQ=255)
                                                                         ctcATAACCTGTAGGGTTCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGTAGCTCATTCCATTCGCAGTGCCGGTTGCGTCAACGCCggtgtt                >  2:7843037/3‑93 (MQ=255)
                                                                           cATAACCAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCt              <  2:1593444/93‑1 (MQ=255)
                                                                                ccAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTg         <  1:5257145/93‑1 (MQ=255)
                                                                                 cAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGt        >  1:7400517/1‑93 (MQ=255)
                                                                                 cAGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGt        >  1:3845249/1‑93 (MQ=255)
                                                                                  aGTAGGGGGCAGGTTGAGCCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTc       <  2:4545476/93‑1 (MQ=255)
                                                                                  aGTAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTc       <  2:4004304/93‑1 (MQ=255)
                                                                                    tAGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCa     <  2:458908/93‑1 (MQ=255)
                                                                                     aGGGGGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAg    <  2:8138691/93‑1 (MQ=255)
                                                                                      ggggGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGt   >  2:1038645/1‑93 (MQ=255)
                                                                                      ggggGCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGt   >  2:2828941/1‑93 (MQ=255)
                                                                                       ggggCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTa  >  2:7722063/1‑93 (MQ=255)
                                                                                       ggggCAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTa  <  1:4121136/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGCCATTACTCGCCTTCATAGTCATCTCATTCTGGTGGCTGTATGTAAGCTGGGTTTGCGGCTCTATATAGAACCCATAACCAGTAGGGGACAGGTTGAACCTCTGCCCGGCTTCCAGGGAGATGCTCATTCCATTCGCAGTGCCGTTGGCGTTAACGCCGTTGTTCTGACTGTCCAGTA  >  NC_000913/1252625‑1252804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑