BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,154,032	T→C	Y250H (TAT→CAT)	yegN →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,154,032	0	T	C	99.3%	1013.8 / ‑4.2	301	Y250H (TAT→CAT)	yegN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (150/149); total (150/151)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 313 total aligned reads displayed.

GCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGG  >  NC_000913/2153942‑2154121

gcTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCAt                                                                                         <  1:4341794/93‑1 (MQ=255)

 cTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATc                                                                                        >  1:5012069/1‑93 (MQ=255)

  tAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATcc                                                                                       >  1:592072/1‑92 (MQ=255)

  tAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCg                                                                                       >  1:7392123/1‑93 (MQ=255)

  tAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCg                                                                                       <  1:2934982/93‑1 (MQ=255)

   aaCGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGc                                                                                      >  1:7788793/1‑93 (MQ=255)

   aaCGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGc                                                                                      >  2:1916124/1‑93 (MQ=255)

    aCGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGcc                                                                                     <  2:6410301/93‑1 (MQ=255)

     cGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCa                                                                                    <  2:5918906/93‑1 (MQ=255)

     cGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCa                                                                                    <  1:4908700/93‑1 (MQ=255)

      gTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAg                                                                                   >  2:7780984/1‑93 (MQ=255)

       ttAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGc                                                                                  <  2:1661856/93‑1 (MQ=255)

        tAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCt                                                                                 <  1:4764765/93‑1 (MQ=255)

         aaCTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTa                                                                                <  1:5881985/93‑1 (MQ=255)

         aaCTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTa                                                                                >  1:8183918/1‑93 (MQ=255)

           cTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAat                                                                              <  1:8256735/93‑1 (MQ=255)

           cTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGT‑‑CGCTTTCTGCGAACACCCACATGCAATCCTCCGAAGAGCATCGCCCGCtcatca                                                                            >  1:4640153/1‑93 (MQ=255)

           cTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGAGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAat                                                                              <  1:8514309/93‑1 (MQ=255)

            tCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTActc                                                                             >  2:7936072/1‑93 (MQ=255)

             cGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAatca                                                                            <  1:7559777/93‑1 (MQ=255)

             cGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAatca                                                                            <  2:8571652/93‑1 (MQ=255)

              ggCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAatcat                                                                           <  1:6132415/93‑1 (MQ=255)

              ggCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAatcat                                                                           <  1:3658739/93‑1 (MQ=255)

               gCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAatcatc                                                                          <  1:7592119/93‑1 (MQ=255)

               gCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAatcatc                                                                          <  1:3117013/93‑1 (MQ=255)

                 aaaaGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGc                                                                        >  1:5301903/1‑93 (MQ=255)

                  aaaGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGcc                                                                       <  1:2844020/93‑1 (MQ=255)

                  aaaGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGcc                                                                       <  1:2695698/93‑1 (MQ=255)

                   aaGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCt                                                                      >  2:5034240/1‑93 (MQ=255)

                     ggTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTAc                                                                    >  2:3687369/1‑93 (MQ=255)

                      gTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTAcc                                                                   >  1:7183778/1‑93 (MQ=255)

                      gTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTAcc                                                                   >  2:5282241/1‑93 (MQ=255)

                       tAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCa                                                                  >  2:102468/1‑93 (MQ=255)

                         gCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGa                                                                >  1:2130765/1‑93 (MQ=255)

                          ccTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCTAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGaa                                                               >  2:6240287/1‑93 (MQ=255)

                          ccTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGaa                                                               <  2:131663/93‑1 (MQ=255)

                           cTCGACGGCCCTTCCCGTTCGGTCACGCTTTCCGCGAGCTGCCAGATGCAATCCGCCGAAGAGCATCGCCATCTAATCATCGCCTACCAGAAc                                                              <  2:4460851/93‑1 (MQ=255)

                           cTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAAc                                                              <  2:2803540/93‑1 (MQ=255)

                            tCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACg                                                             >  1:8434049/1‑93 (MQ=255)

                            tCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATAGCCTACCAGAACg                                                             >  1:1079681/1‑93 (MQ=255)

                             cGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACgg                                                            <  1:6549111/93‑1 (MQ=255)

                             cGACGGCCATTCCCGTGCGGTCACGCTTTCCGCCAACGACCAGATGCAATCCGCAGAAGAGCATCGCCAGCTAATCATCGCCTACAAGAACgg                                                            <  1:4406852/93‑1 (MQ=255)

                              gACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGgc                                                           <  2:1725178/93‑1 (MQ=255)

                              gACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGgc                                                           <  1:6669615/93‑1 (MQ=255)

                               aCGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGgcg                                                          <  1:2520648/93‑1 (MQ=255)

                               aCGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTACTCATCGCCTACCAGAACGgcg                                                          >  1:3478212/1‑93 (MQ=255)

                                 ggCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGgcgcg                                                        >  1:3779719/1‑93 (MQ=255)

                                  gCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGgcgcgc                                                       >  1:5478982/1‑93 (MQ=255)

                                   cccTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGcc                                                      >  2:5419972/1‑93 (MQ=255)

                                   cccTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGcc                                                      <  2:3581312/93‑1 (MQ=255)

                                   cccTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGcc                                                      <  1:3896775/93‑1 (MQ=255)

                                   cccTGCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGcc                                                      <  2:2233115/93‑1 (MQ=255)

                                    ccTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCa                                                     >  2:6712741/1‑93 (MQ=255)

                                    ccTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCa                                                     >  2:7698070/1‑93 (MQ=255)

                                     cTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCaa                                                    >  2:1152513/1‑93 (MQ=255)

                                     cTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCaa                                                    <  2:2494496/93‑1 (MQ=255)

                                        cccGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTc                                                 <  2:3516274/93‑1 (MQ=255)

                                        cccGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTc                                                 >  1:6842277/1‑93 (MQ=255)

                                           gTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTc                                              <  1:4046808/93‑1 (MQ=255)

                                            tGCGGTCACGCTGTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCt                                             <  1:7349109/93‑1 (MQ=255)

                                             gCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTg                                            <  1:8656959/93‑1 (MQ=255)

                                                gTCACGCTTTCCTCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGc                                         >  2:2328100/1‑93 (MQ=255)

                                                gTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGc                                         <  1:1255577/93‑1 (MQ=255)

                                                 tCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCg                                        >  1:4310488/1‑93 (MQ=255)

                                                   aCGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGAt                                      >  1:5955828/1‑93 (MQ=255)

                                                    cGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATg                                     >  2:2416163/1‑93 (MQ=255)

                                                    cGCTTTCCGCGAACGACCAGAGGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATg                                     <  1:3752763/93‑1 (MQ=255)

                                                     gCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATgc                                    >  2:1330535/1‑92 (MQ=255)

                                                     ccTTTCCGCGAACGACCAGATGCAAACCGGCGAAGAGCATCGCCAGGTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGt                                    <  1:2431027/92‑1 (MQ=255)

                                                       tttCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCg                                  >  2:2610367/1‑93 (MQ=255)

                                                       cttCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCg                                  <  1:7671284/92‑1 (MQ=255)

                                                          ccGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCTAATTCGTCTGGGCGATGTCGCaa                               >  2:5739928/1‑93 (MQ=255)

                                                          ccGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCaa                               <  1:2199055/93‑1 (MQ=255)

                                                             cgAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTg                            >  2:3092325/1‑93 (MQ=255)

                                                              gAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGt                           <  1:2271283/93‑1 (MQ=255)

                                                              gAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGt                           >  2:3276203/1‑93 (MQ=255)

                                                               aaCGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTa                          >  2:4024360/1‑93 (MQ=255)

                                                               aaCGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCAAATTCGTCTGGGCGATGTCGCAACTGTa                          <  2:4483587/93‑1 (MQ=255)

                                                                aCGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTag                         <  2:1599749/93‑1 (MQ=255)

                                                                   aCCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGc                      <  2:487082/93‑1 (MQ=255)

                                                                     cAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCTCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCaa                    >  1:1799142/1‑93 (MQ=255)

                                                                     cAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCaa                    >  1:625821/1‑93 (MQ=255)

                                                                     cAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCaa                    <  2:2707666/93‑1 (MQ=255)

                                                                      aGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAg                   >  2:7070589/1‑93 (MQ=255)

                                                                         tGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTg                <  1:1399657/93‑1 (MQ=255)

                                                                           cAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCa              >  2:8298912/1‑93 (MQ=255)

                                                                           cAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCa              >  2:8115847/1‑93 (MQ=255)

                                                                            aaTCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAg             >  1:8524211/1‑93 (MQ=255)

                                                                            aaTCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAg             >  2:7261011/1‑93 (MQ=255)

                                                                             aTCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGa            >  2:6064781/1‑93 (MQ=255)

                                                                             aTCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGa            >  1:8079945/1‑93 (MQ=255)

                                                                                cgccgAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGAGCAGaaaa         >  2:6801738/1‑93 (MQ=255)

                                                                                    gAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGc     <  1:1989874/93‑1 (MQ=255)

                                                                                    gAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGc     >  1:3073931/1‑93 (MQ=255)

                                                                                     aaGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCt    <  2:8484025/93‑1 (MQ=255)

                                                                                     aaGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCt    <  2:4213258/93‑1 (MQ=255)

                                                                                     aaGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCt    <  1:5193205/93‑1 (MQ=255)

                                                                                     aaGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCt    <  2:1267232/93‑1 (MQ=255)

                                                                                      agagCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTg   <  1:6915835/93‑1 (MQ=255)

                                                                                       gagCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCttc  >  2:7587117/1‑91 (MQ=255)

GCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGG  >  NC_000913/2153942‑2154121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`