BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	323,407	A→G	G142G (GGA→GGG)	ykgG →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	323,407	0	A	G	99.0%	1020.1 / ‑1.7	304	G142G (GGA→GGG)	ykgG	putative transporter
Reads supporting (aligned to +/- strand): ref base A (0/3); new base G (147/154); total (147/157)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 327 total aligned reads displayed.

GGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTAT  >  NC_000913/323317‑323496

 gATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGc                                                                                        <  2:6274794/93‑1 (MQ=255)

 gATCAGGCGAAAGGTGCCGATGATATCTCGCAGATAGAGCAGGCTAAAGTGGGTGTCGGGTATGCTGAATATGGTTTAACCGAATCGGGGGGc                                                                                        <  2:7340631/93‑1 (MQ=255)

   tCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGt                                                                                      >  1:7749771/1‑93 (MQ=255)

      ggCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGt                                                                                   >  2:4195259/1‑93 (MQ=255)

      ggCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGCCGTGGt                                                                                   >  1:1139329/1‑93 (MQ=255)

       gCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGtt                                                                                  <  2:1551119/93‑1 (MQ=255)

        cGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTc                                                                                 >  2:3208346/1‑93 (MQ=255)

        cGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTc                                                                                 <  1:4742472/93‑1 (MQ=255)

         gAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCt                                                                                >  1:5967257/1‑93 (MQ=255)

         gAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCt                                                                                >  1:1025403/1‑93 (MQ=255)

         gAAAGGGTCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCt                                                                                <  1:8335967/93‑1 (MQ=255)

          aaaTGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtt                                                                               <  2:6336720/93‑1 (MQ=255)

          aaaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtt                                                                               <  1:6632541/93‑1 (MQ=255)

           aaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGACTATGGTTTAACCGAATCGGGGGGCGCGGCTCttt                                                                              >  1:577151/1‑93 (MQ=255)

           aaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCttt                                                                              >  2:2557432/1‑93 (MQ=255)

            aGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtttt                                                                             >  1:881956/1‑93 (MQ=255)

             ggTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCttttt                                                                            >  2:7457651/1‑93 (MQ=255)

             ggTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGGGTTGTGTATGCTGAATATGGTTTAGCCGAATCGGGGGGCGGGGtttttttt                                                                            >  2:7579259/1‑93 (MQ=255)

               tGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTc                                                                          <  1:3447854/93‑1 (MQ=255)

                gCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTcc                                                                         <  1:3525764/93‑1 (MQ=255)

                  cGAGAATATCTCGCAGGCAGAGCAGTCTAAAGTGGGTGTTGTGTATGCTAAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgc                                                                       <  2:1110161/93‑1 (MQ=255)

                  cGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgc                                                                       >  1:3675839/1‑93 (MQ=255)

                   gagaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgcc                                                                      >  1:407620/1‑93 (MQ=255)

                   gagaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgcc                                                                      >  2:5964303/1‑93 (MQ=255)

                   gagaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgcc                                                                      <  1:4454862/93‑1 (MQ=255)

                   gagaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgcc                                                                      >  1:2819585/1‑93 (MQ=255)

                    agaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccg                                                                     <  2:3287073/93‑1 (MQ=255)

                     gaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgc                                                                    >  2:3056301/1‑93 (MQ=255)

                     gaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgc                                                                    <  2:709315/93‑1 (MQ=255)

                     agatatCTCGCAGGCAGAGCAGGCTAAAGGGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgc                                                                    <  1:2193157/91‑1 (MQ=255)

                       atatCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgccg                                                                  >  2:846021/1‑93 (MQ=255)

                       atatCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgccg                                                                  <  2:5653110/93‑1 (MQ=255)

                        tatCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGa                                                                 <  1:3220633/93‑1 (MQ=255)

                         atCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAg                                                                >  1:49278/1‑93 (MQ=255)

                         atCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAg                                                                >  1:3310852/1‑93 (MQ=255)

                          tctcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGATCTTTTTTccgccgccgggg                                                               >  2:4691398/1‑90 (MQ=255)

                           ctcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAgcg                                                              <  1:7029990/93‑1 (MQ=255)

                            tcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAgcgc                                                             >  2:6447828/1‑93 (MQ=255)

                             cGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGcgcg                                                            <  2:6702011/93‑1 (MQ=255)

                              gcaggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCgg                                                           >  2:2323739/1‑93 (MQ=255)

                               gagggagCGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTACCGCCGCCGAGCGCggg                                                          <  1:2898307/92‑1 (MQ=255)

                                 ggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCg                                                        >  1:6177785/1‑93 (MQ=255)

                                 ggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCg                                                        >  1:1818417/1‑93 (MQ=255)

                                 ggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCg                                                        >  1:8688966/1‑93 (MQ=255)

                                  gcagAGCAGGCTAAAGTGGGTTTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGt                                                       <  2:2129952/93‑1 (MQ=255)

                                   cagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGtt                                                      <  2:5414184/93‑1 (MQ=255)

                                   cagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGtt                                                      >  2:8638303/1‑93 (MQ=255)

                                   cagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGtt                                                      <  1:2687484/93‑1 (MQ=255)

                                    agagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTc                                                     <  2:3681876/93‑1 (MQ=255)

                                    agagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTc                                                     <  2:283186/93‑1 (MQ=255)

                                     gagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCa                                                    <  2:7526983/93‑1 (MQ=255)

                                     gagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCa                                                    >  1:5017965/1‑93 (MQ=255)

                                     gagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCa                                                    <  1:8044676/93‑1 (MQ=255)

                                      agCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCAt                                                   >  1:161708/1‑93 (MQ=255)

                                       gCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCAtt                                                  >  2:6945284/1‑93 (MQ=255)

                                         aGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGa                                                <  1:6894754/93‑1 (MQ=255)

                                         aGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGa                                                >  2:3866765/1‑93 (MQ=255)

                                         aGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGa                                                <  1:6519585/93‑1 (MQ=255)

                                         aGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGa                                                <  2:2190332/93‑1 (MQ=255)

                                            cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                             <  1:8516463/93‑1 (MQ=255)

                                            cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                             >  1:3768696/1‑93 (MQ=255)

                                            cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                             <  1:383422/93‑1 (MQ=255)

                                             gaaaGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCt                                            <  2:6872208/92‑1 (MQ=255)

                                               aaGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATCGAGCCTGc                                          <  1:3028567/93‑1 (MQ=255)

                                                  tGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTcc                                       <  2:13978/93‑1 (MQ=255)

                                                  tGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTcc                                       <  1:1885539/93‑1 (MQ=255)

                                                   gggTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTccc                                      <  1:5545206/93‑1 (MQ=255)

                                                    ggTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCg                                     >  2:3404847/1‑93 (MQ=255)

                                                    ggTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCg                                     <  2:4387987/93‑1 (MQ=255)

                                                    ggTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCg                                     <  1:2341288/93‑1 (MQ=255)

                                                       gttgtGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGaa                                  <  2:6735355/93‑1 (MQ=255)

                                                        ttgtGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAat                                 >  1:3068123/1‑93 (MQ=255)

                                                          gtgtATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAatat                               <  2:1274839/93‑1 (MQ=255)

                                                             tATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATtct                            >  1:8620871/1‑93 (MQ=255)

                                                             tATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATtct                            >  1:2009841/1‑93 (MQ=255)

                                                               tGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATtctgt                          >  1:7348391/1‑91 (MQ=255)

                                                               tGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTctct                          <  2:1895723/93‑1 (MQ=255)

                                                               tGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTctct                          <  2:4311597/93‑1 (MQ=255)

                                                               tGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTctct                          >  2:5033014/1‑93 (MQ=255)

                                                                  tGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTTCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGCATATTCTCtttt                       >  2:8272969/1‑93 (MQ=255)

                                                                   gAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCttttt                      <  1:7699537/93‑1 (MQ=255)

                                                                    aaTATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTGCGCCGCCGAGCGCGGGGGTTCATTGAGCCGGTTCCGGGAATATTCACTTTCTa                     >  2:2629951/1‑93 (MQ=255)

                                                                    aaTATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTa                     <  2:6171671/93‑1 (MQ=255)

                                                                    aaTATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTa                     <  1:5307205/93‑1 (MQ=255)

                                                                     atatGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTAt                    <  1:4146762/93‑1 (MQ=255)

                                                                      tatGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATc                   >  1:7456584/1‑93 (MQ=255)

                                                                       atGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATcc                  >  1:4357009/1‑93 (MQ=255)

                                                                        tGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCt                 >  2:7986302/1‑93 (MQ=255)

                                                                        tGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCt                 <  2:3975011/93‑1 (MQ=255)

                                                                         ggTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTg                >  1:8743277/1‑93 (MQ=255)

                                                                             tAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCATTTTATCCTGCGTa            >  2:2764839/1‑93 (MQ=255)

                                                                               aCCGATTCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa          <  1:8266300/93‑1 (MQ=255)

                                                                               aCCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa          >  1:6566373/1‑93 (MQ=255)

                                                                                ccGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaaa         <  2:326364/93‑1 (MQ=255)

                                                                                  gAATCGGGGGGCTTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGc       <  1:6398128/93‑1 (MQ=255)

                                                                                    aTCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCAc     <  1:4789661/93‑1 (MQ=255)

                                                                                      cGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTa   <  1:4322334/93‑1 (MQ=255)

                                                                                      cGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTa   >  1:5728168/1‑93 (MQ=255)

                                                                                      cGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTa   <  1:7196306/93‑1 (MQ=255)

                                                                                       ggggggCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTAt  <  1:1420273/93‑1 (MQ=255)

GGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTAT  >  NC_000913/323317‑323496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`