BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,021,684	Δ1 bp	coding (542/609 nt)\|coding (1/243 nt)	b4556 → tatD →	hypothetical protein Mg‑dependent DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,021,683	0	C	.	99.6%	1034.2 / ‑3.9	221	coding (541/609 nt)	b4556	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (98/122); minor base G (0/1); total (98/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTC  >  NC_000913/4021591‑4021771

ttcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAc                                                                                          <  1:5968786/93‑1 (MQ=255)

ttcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAc                                                                                          >  1:179875/1‑93 (MQ=255)

 tcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcg                                                                                         <  1:1884618/93‑2 (MQ=255)

 tcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcg                                                                                         >  2:483314/1‑92 (MQ=255)

  cttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcga                                                                                        <  1:802566/93‑3 (MQ=255)

   ttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgac                                                                                       >  1:5992265/1‑90 (MQ=255)

    tcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgacg                                                                                      >  1:3439456/1‑89 (MQ=255)

    tcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgacg                                                                                      <  2:5724903/93‑5 (MQ=255)

    tcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgacg                                                                                      >  1:337499/1‑89 (MQ=255)

        tGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGa                                                                                 <  2:1658591/93‑1 (MQ=255)

         gCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGAc                                                                                >  2:6112542/1‑93 (MQ=255)

         gCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGAc                                                                                <  1:3794235/93‑1 (MQ=255)

          cTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACt                                                                               >  2:2062345/1‑93 (MQ=255)

           tttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTg                                                                              <  1:4932014/93‑1 (MQ=255)

           tttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTg                                                                              <  2:4161869/93‑1 (MQ=255)

            ttACCGGCACACGCGAAGATATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTgg                                                                             <  1:1132846/93‑1 (MQ=255)

            ttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTgg                                                                             <  1:5105918/93‑1 (MQ=255)

             tACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGa                                                                            <  1:5721740/93‑1 (MQ=255)

              aCCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAg                                                                           >  1:5536817/1‑93 (MQ=255)

                cGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCt                                                                         <  2:5168980/93‑1 (MQ=255)

                  gCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                       <  1:691480/93‑1 (MQ=255)

                  gCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                       <  2:3920443/93‑1 (MQ=255)

                    acacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCgg                                                                     >  2:5019231/1‑93 (MQ=255)

                    acacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCgg                                                                     <  1:5394980/93‑1 (MQ=255)

                     cacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCggg                                                                    >  1:4201346/1‑93 (MQ=255)

                      acGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGa                                                                   >  1:1537466/1‑93 (MQ=255)

                       cgcgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGaa                                                                  <  2:3819054/93‑1 (MQ=255)

                       cgcgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGaa                                                                  <  2:1365611/93‑1 (MQ=255)

                        gcgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGaaa                                                                 <  1:4502831/93‑2 (MQ=255)

                        gcgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAAc                                                                 <  2:3280184/93‑1 (MQ=255)

                         cgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACt                                                                >  1:3268277/1‑93 (MQ=255)

                         cgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACt                                                                <  1:667863/93‑1 (MQ=255)

                          gAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtt                                                               <  1:2287495/93‑1 (MQ=255)

                          gAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtt                                                               <  1:3219060/93‑1 (MQ=255)

                           aaGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACttt                                                              <  2:602687/93‑1 (MQ=255)

                           aaGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACttt                                                              >  1:780699/1‑93 (MQ=255)

                           aaGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACttt                                                              >  2:1142676/1‑93 (MQ=255)

                            agagATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtttt                                                             >  2:781812/1‑93 (MQ=255)

                             gagaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTg                                                            >  1:6254185/1‑93 (MQ=255)

                                 tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGt                                                        <  2:5090849/93‑1 (MQ=255)

                                 tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGt                                                        <  1:6063091/93‑1 (MQ=255)

                                 tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGt                                                        <  1:1710004/93‑1 (MQ=255)

                                  gCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGtt                                                       <  2:5207975/93‑1 (MQ=255)

                                  gCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGtt                                                       >  2:6402185/1‑93 (MQ=255)

                                      gcgtgcgtGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAtt                                                   <  2:5865185/93‑1 (MQ=255)

                                       cgtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTc                                                  <  1:4877751/93‑1 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                 <  2:889228/93‑1 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                 >  1:465413/1‑93 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                 <  2:451423/93‑1 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                 >  1:4623127/1‑93 (MQ=255)

                                          gcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcgg                                               <  2:3459512/93‑1 (MQ=255)

                                          gcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcgg                                               >  2:910122/1‑93 (MQ=255)

                                          gcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcgg                                               <  1:1509959/93‑1 (MQ=255)

                                           cgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcggc                                              >  2:1533723/1‑93 (MQ=255)

                                                 acATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaaa                                        >  2:6089354/2‑93 (MQ=255)

                                                  cATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaaaa                                       >  2:1822924/1‑93 (MQ=255)

                                                    tGGAATTTATATCAGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGGACTTTTGCCGTTGATTCCGGCGGAAAAAtt                                     >  2:3967122/1‑93 (MQ=255)

                                                     ggAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGGAAAATTa                                    >  1:4805413/1‑93 (MQ=255)

                                                      gAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAc                                   >  1:1891156/1‑93 (MQ=255)

                                                       aaTTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACt                                  <  1:2240952/93‑1 (MQ=255)

                                                        aTTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTg                                 <  1:1445552/93‑1 (MQ=255)

                                                         tttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGa                                <  1:2207523/93‑1 (MQ=255)

                                                         tttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGa                                >  1:2018430/1‑93 (MQ=255)

                                                          ttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAt                               <  1:1303035/93‑1 (MQ=255)

                                                          ttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAt                               <  2:1266007/93‑1 (MQ=255)

                                                           tataTCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATc                              <  1:3094648/93‑1 (MQ=255)

                                                           tataTCGGCATTACCGGTTGGGTTTGCGATG‑AGCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATc                              <  1:2791283/93‑1 (MQ=255)

                                                             tatCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGa                            >  2:2027926/1‑93 (MQ=255)

                                                             tatCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGa                            >  1:995589/1‑93 (MQ=255)

                                                                cGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAAc                         <  2:2245992/93‑1 (MQ=255)

                                                                cGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAAc                         >  1:2643014/1‑93 (MQ=255)

                                                                   cATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGa                      <  1:2540190/93‑1 (MQ=255)

                                                                      tACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgc                   >  1:4252472/1‑93 (MQ=255)

                                                                       aCCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgcg                  >  2:5310166/1‑93 (MQ=255)

                                                                        ccGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGACACTGATgcgc                 >  2:676089/1‑93 (MQ=255)

                                                                        ccGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgcgc                 >  2:5407011/1‑93 (MQ=255)

                                                                        ccGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgcgc                 >  1:567837/1‑93 (MQ=255)

                                                                         cGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGcc                <  1:1034810/93‑1 (MQ=255)

                                                                         cGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGcc                <  2:2817574/93‑1 (MQ=255)

                                                                         cGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGcc                <  2:2553175/93‑1 (MQ=255)

                                                                          ggTTGGGTTTGCGATGAA‑CGACGCGGACTGGGGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCg               >  2:1699842/1‑93 (MQ=255)

                                                                          ggTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCg               >  1:2993188/1‑93 (MQ=255)

                                                                          ggTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCg               >  2:2870803/1‑93 (MQ=255)

                                                                           gTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGt              <  1:2084719/93‑1 (MQ=255)

                                                                            ttGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTa             <  2:4303654/93‑1 (MQ=255)

                                                                            ttGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTa             <  1:5012539/93‑1 (MQ=255)

                                                                              gggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATc           <  1:1389656/93‑1 (MQ=255)

                                                                              gggTGTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATc           <  1:5938682/93‑1 (MQ=255)

                                                                               ggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCt          <  1:3620445/93‑1 (MQ=255)

                                                                                    gCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTcc     >  1:1683066/1‑93 (MQ=255)

                                                                                     cGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTccc    <  1:6452519/93‑1 (MQ=255)

                                                                                      gATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCt   >  2:3651473/1‑93 (MQ=255)

                                                                                      gATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCt   <  1:5205129/93‑1 (MQ=255)

                                                                                      gATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCt   <  1:2929504/93‑1 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  >  2:5794773/1‑93 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  >  2:213783/1‑93 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  >  2:5279590/1‑93 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  <  2:4667009/93‑1 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  <  1:1173599/93‑1 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGCAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  <  2:3050819/93‑1 (MQ=255)

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTC  >  NC_000913/4021591‑4021771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`