BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,651	(G)_5→4	coding (1008/1548 nt)	yjeF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,647	0	G	.	93.6%	830.3 / 42.3	203	coding (1004/1548 nt)	yjeF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (8/5); new base . (94/96); total (102/101)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.70e-01

Only 100 of 218 total aligned reads displayed.

GATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCC  >  NC_000913/4392555‑4392735

gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTg                                                                                          <  2:2838551/93‑1 (MQ=255)

gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTg                                                                                          <  1:5006253/93‑1 (MQ=255)

 aTGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                                                                                         >  2:3071679/1‑93 (MQ=255)

 aTGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                                                                                         <  1:3000966/93‑1 (MQ=255)

  tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                                                                                        <  1:5716886/93‑1 (MQ=255)

  tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                                                                                        <  1:6164189/93‑1 (MQ=255)

   ggTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCGGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgggg                                                                                       <  2:3165980/93‑1 (MQ=255)

    gTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgggga                                                                                      <  2:3669185/93‑2 (MQ=255)

      gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaa                                                                                    <  2:5908533/93‑1 (MQ=255)

      gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaa                                                                                    >  1:3769880/1‑93 (MQ=255)

       cgtGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaa                                                                                   >  2:6247353/3‑93 (MQ=255)

       cATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaa                                                                                   <  1:1946417/93‑1 (MQ=255)

         tGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaaaa                                                                                 <  1:5090451/93‑1 (MQ=255)

         tGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaaaa                                                                                 <  2:2239240/93‑1 (MQ=255)

          gAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaaaag                                                                                >  1:4051218/1‑92 (MQ=255)

           aaCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGc                                                                              <  1:10048/93‑1 (MQ=255)

           aaCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGc                                                                              <  1:1286922/93‑1 (MQ=255)

            aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGATGATTGGTCCCGGTCTGGGCCAGAAAGAGT‑GGGGAAAAAAGCa                                                                             <  1:6302432/93‑1 (MQ=255)

             cTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCAc                                                                            <  1:4508847/93‑1 (MQ=255)

             cTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCAc                                                                            >  1:2875045/1‑93 (MQ=255)

              tGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACt                                                                           >  2:2549372/1‑93 (MQ=255)

                aCGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGc                                                                         >  2:5573798/1‑93 (MQ=255)

                 cGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCa                                                                        <  2:5041187/93‑1 (MQ=255)

                  gATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaa                                                                       >  2:5787002/1‑93 (MQ=255)

                   aTGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaa                                                                      >  2:4436523/1‑93 (MQ=255)

                    tGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaa                                                                     >  2:4665811/1‑93 (MQ=255)

                    tGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaa                                                                     >  1:6404266/1‑93 (MQ=255)

                    tGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaa                                                                     <  2:1058065/93‑1 (MQ=255)

                    tGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaa                                                                     <  2:3252880/93‑1 (MQ=255)

                     ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaaa                                                                    <  2:1910882/93‑1 (MQ=255)

                     ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaaa                                                                    >  1:1275480/1‑93 (MQ=255)

                      gACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAg                                                                   <  2:1330597/93‑1 (MQ=255)

                        ctctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGtt                                                                 <  1:5288780/93‑1 (MQ=255)

                        ctctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGtt                                                                 >  1:2253037/1‑93 (MQ=255)

                         tctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTg                                                                >  2:3513136/1‑93 (MQ=255)

                         tctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTg                                                                >  1:3502963/1‑93 (MQ=255)

                          ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTga                                                               <  2:1449254/93‑1 (MQ=255)

                          ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTga                                                               >  2:5737873/1‑93 (MQ=255)

                           tctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGACCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTgag                                                              >  2:4531930/1‑93 (MQ=255)

                              tACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAt                                                           <  1:5484978/93‑1 (MQ=255)

                              tACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAt                                                           >  2:1839000/1‑93 (MQ=255)

                               aCCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtt                                                          >  2:5292965/1‑93 (MQ=255)

                               aCCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtt                                                          <  2:1623787/93‑1 (MQ=255)

                               aCCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtt                                                          >  2:806953/1‑93 (MQ=255)

                               aCCGAAAGCCTGGAAGGGGCCGATGTCGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtt                                                          <  1:2529550/93‑1 (MQ=255)

                                ccGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAttt                                                         >  1:2838960/1‑93 (MQ=255)

                                    aaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGc                                                     >  2:320957/1‑93 (MQ=255)

                                     aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCa                                                    >  1:2068693/1‑93 (MQ=255)

                                      gCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaa                                                   <  2:403368/93‑1 (MQ=255)

                                         tGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc                                                >  1:316498/1‑93 (MQ=255)

                                         tGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc                                                >  1:1435522/1‑93 (MQ=255)

                                          ggAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                               >  1:6089986/1‑93 (MQ=255)

                                           gAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGa                                              <  2:1156931/93‑1 (MQ=255)

                                           gAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGa                                              <  1:3852003/93‑1 (MQ=255)

                                           gAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGa                                              >  1:5822869/1‑93 (MQ=255)

                                           gAATGGGCCGATGTGGTGGTGATTGGTCCCGATCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGa                                              <  2:5234891/93‑1 (MQ=255)

                                            aaTGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAt                                             >  1:5756331/1‑93 (MQ=255)

                                             aTGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtg                                            >  2:939375/1‑93 (MQ=255)

                                             aTGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtg                                            >  1:4496085/1‑93 (MQ=255)

                                             aTGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtg                                            >  2:2048727/1‑93 (MQ=255)

                                                ggCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTGTCGCAAACCGAtgttg                                         <  1:1564914/93‑1 (MQ=255)

                                                 gCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgttgt                                        >  2:5944923/1‑93 (MQ=255)

                                                  ccGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg                                       >  1:5180086/1‑93 (MQ=255)

                                                   cGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGAAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg                                      <  2:391606/93‑1 (MQ=255)

                                                    gATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTggg                                     >  2:889974/1‑93 (MQ=255)

                                                    gATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTggg                                     <  1:164264/93‑1 (MQ=255)

                                                     aTGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                    >  1:4815070/1‑93 (MQ=255)

                                                     aTGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                    <  1:4682994/93‑1 (MQ=255)

                                                      tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGAt                                   >  1:5349241/1‑93 (MQ=255)

                                                      tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGAt                                   <  2:4796573/93‑1 (MQ=255)

                                                      tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGAt                                   >  1:3980605/1‑93 (MQ=255)

                                                        tggtggtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGc                                 <  1:3388059/93‑1 (MQ=255)

                                                          gtggtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg                               >  1:24243/1‑93 (MQ=255)

                                                           tggtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGa                              <  1:5910557/93‑1 (MQ=255)

                                                            ggtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGAt                             <  2:495012/93‑1 (MQ=255)

                                                             gtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATg                            <  1:3453868/93‑1 (MQ=255)

                                                                  tGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTg                       <  1:4715621/93‑1 (MQ=255)

                                                                  tGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTg                       <  2:4734232/93‑1 (MQ=255)

                                                                   ggTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGa                      <  2:2386537/93‑1 (MQ=255)

                                                                   ggTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGa                      <  2:3410012/93‑1 (MQ=255)

                                                                    gTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGaa                     >  1:1722805/1‑93 (MQ=255)

                                                                     tCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAAc                    >  1:4566450/1‑93 (MQ=255)

                                                                      cccGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAAcc                   <  2:3706643/93‑1 (MQ=255)

                                                                       ccGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACct                  >  1:2341363/1‑93 (MQ=255)

                                                                         ggTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgc                <  2:6111351/93‑1 (MQ=255)

                                                                         ggTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgc                <  2:1347081/93‑1 (MQ=255)

                                                                          gTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgct               >  2:1283132/1‑93 (MQ=255)

                                                                          gTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgct               >  2:1851336/1‑93 (MQ=255)

                                                                          gTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgct               >  2:6256670/1‑93 (MQ=255)

                                                                             tGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGc            <  2:5425414/93‑1 (MQ=255)

                                                                              gggCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGca           >  2:4087432/1‑93 (MQ=255)

                                                                              gggCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGca           >  2:1071829/1‑93 (MQ=255)

                                                                               ggCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaa          <  1:4347081/93‑1 (MQ=255)

                                                                               ggCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaa          <  2:3127537/93‑1 (MQ=255)

                                                                                 ccAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatc        <  1:3907425/93‑1 (MQ=255)

                                                                                   aGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaa      <  2:1730018/93‑1 (MQ=255)

                                                                                   aGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaa      >  1:3612430/1‑93 (MQ=255)

                                                                                      aaGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATcc   >  1:637862/1‑93 (MQ=255)

                                                                                       agagT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGACCCTGCTGGCAATCAATccc  <  2:2248432/93‑1 (MQ=255)

                                                                                       agagT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATccc  >  2:1266532/1‑93 (MQ=255)

GATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCC  >  NC_000913/4392555‑4392735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`