Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,041,671	(T)6→7	intergenic (+46/‑108)	dsbA → / → b3861	protein disulfide isomerase I/putative GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,041,665	1	.	T	100.0%	33.5 / NA	10	intergenic (+40/‑114)	dsbA/b3861	protein disulfide isomerase I/putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (2/8);  total (2/8)

TTTTTGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAGTATTCACGGGC‑TTTTTTATTATTTAATAAATATAAATACATTCTGATAATGCATCCTGCCGCTGGACATTATTTCAGCATAAGTGGATA  >  NC_000913/4041573‑4041743                                                                                              |                                                                               tttttGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGc                                                                                 <  2:1080667/93‑1 (MQ=255)   tttGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑tt                                                                              <  1:1692732/93‑1 (MQ=255)   tttGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑tt                                                                              <  1:4842709/93‑1 (MQ=255)    ttGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttt                                                                             <  2:755331/93‑1 (MQ=255)    ttGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttt                                                                             <  2:3102815/93‑1 (MQ=255)    ttGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttt                                                                             <  2:1221780/93‑1 (MQ=255)    ttGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttt                                                                             <  1:5949357/93‑1 (MQ=255)    ttGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttt                                                                             <  1:2065501/93‑1 (MQ=255)      gTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttttt                                                                           <  1:3262458/93‑1 (MQ=255)      gTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttttt                                                                           <  1:393587/93‑1 (MQ=255)      gTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttttt                                                                           <  1:2861331/93‑1 (MQ=255)        tCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttttttt                                                                         <  2:5165963/93‑2 (MQ=255)         cagcagTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑ttttttta                                                                        <  1:2594272/93‑3 (MQ=255)           gcagTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGC‑tttttttatt                                                                      <  1:4340491/93‑5 (MQ=255)             agTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGCTTTTTTtattat                                                                     <  1:4981576/93‑1 (MQ=255)             agTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGCTTTTTTtattat                                                                     <  1:5489698/93‑1 (MQ=255)              gTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGCTTTTTTTattatt                                                                    <  2:266809/93‑1 (MQ=255)               tATGCTGATCCAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGCTTTTTTTATTAttt                                                                   <  1:1692147/93‑1 (MQ=255)                aTGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGCTTTTTTTATTATTta                                                                  <  2:3077644/93‑1 (MQ=255)                 tGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGCTTTTTTTATTATTtaa                                                                 <  1:3067001/93‑1 (MQ=255)                                atatCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAATATTCACGGGCTTTTTTTATTATTTAATAAATATAAATACAt                                                  <  2:2777324/93‑1 (MQ=255)                                                        cAATGTAAATTACATAAAGCCCGTGAATATTCACGGGCTTTTTTTATTATTTAATAAATATAAATACATTCTGATAATGCATCCTGCCGCTgg                          <  2:1976598/93‑1 (MQ=255)                                                        cAATGTAAATTACATAAAGCCCGTGAATATTCACGGGCTTTTTTTATTATTTAATAAATATAAATACATTCTGATAATGCATCCTGCCGCTgg                          >  1:3390042/1‑93 (MQ=255)                                                                                gaatATTCACGGGCTTTTTTTATTATTTAATAAATATAAATACATTCTGATAATGCATCCTGCCGCTGGACATTATTTCAGCATAAGTGGata  >  1:3189860/4‑93 (MQ=255)                                                                                              |                                                                               TTTTTGTTCAGCAGTATGCTGATACAGTGAAATATCTGTCCGAGAAAAAATAATTCAATGTAAATTACATAAAGCCCGTGAGTATTCACGGGC‑TTTTTTATTATTTAATAAATATAAATACATTCTGATAATGCATCCTGCCGCTGGACATTATTTCAGCATAAGTGGATA  >  NC_000913/4041573‑4041743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑