Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,448,543 +C intergenic (+48/‑90) ytfQ → / → ytfR putative LACI‑type transcriptional regulator/YtfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,448,5421.C100.0% 624.8 / NA 210intergenic (+47/‑91)ytfQ/ytfRputative LACI‑type transcriptional regulator/YtfR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (135/75);  total (135/75)

Only 100 of 223 total aligned reads displayed.

AGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCG‑G‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG  >  NC_000913/4448462‑4448623
                                                                                  |                                                                                  
agaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGc                                                                         >  1:5121479/1‑93 (MQ=37)
agaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGc                                                                         >  1:3502656/1‑93 (MQ=37)
 gaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCg                                                                        >  2:5919096/1‑93 (MQ=37)
   aTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCTATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGAt                                                                      >  1:3261779/1‑93 (MQ=25)
   aTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGAt                                                                      <  2:5275848/93‑1 (MQ=37)
   aTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGAt                                                                      >  2:1532589/1‑93 (MQ=37)
     tAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATgg                                                                    <  1:1855677/93‑1 (MQ=37)
      agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGt                                                                   >  2:6316705/1‑93 (MQ=37)
      agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGt                                                                   <  1:455451/93‑1 (MQ=37)
      agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGt                                                                   <  1:3012791/93‑1 (MQ=37)
      agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGt                                                                   >  2:2805107/1‑93 (MQ=37)
       gagaAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATggcg                                                                  >  2:5392166/1‑91 (MQ=25)
       gagaAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTg                                                                  >  1:5463264/1‑93 (MQ=37)
       gagaAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTg                                                                  >  2:4934388/1‑93 (MQ=37)
        agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGc                                                                 >  1:3345042/1‑93 (MQ=37)
        agaagaagaAAAATAGGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGc                                                                 <  2:2413753/93‑1 (MQ=25)
         gaagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCa                                                                <  2:4212691/93‑1 (MQ=37)
         gaagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCa                                                                >  2:4089406/1‑93 (MQ=37)
         gaagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCa                                                                >  1:6115491/1‑93 (MQ=37)
          aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCAc                                                               >  1:592350/1‑93 (MQ=37)
          aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCAc                                                               >  1:2048366/1‑93 (MQ=37)
          aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCAc                                                               >  1:3863995/1‑93 (MQ=37)
           agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACt                                                              >  2:5214200/1‑93 (MQ=37)
           agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACt                                                              >  2:6145389/1‑93 (MQ=37)
            gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTg                                                             >  1:4272003/1‑93 (MQ=37)
              agaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGaa                                                           >  2:3467055/1‑93 (MQ=37)
                aaaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGc                                                         >  2:5166983/1‑93 (MQ=37)
                 aaaaTATGGGGTATGGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGcc                                                        <  2:4569262/93‑1 (MQ=25)
                   aaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTg                                                      <  2:2077199/93‑1 (MQ=37)
                   aaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTg                                                      >  2:5739251/1‑93 (MQ=37)
                    atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGa                                                     <  2:2972506/93‑1 (MQ=37)
                    atatGGGGTATGGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAGGGCGCTCGCGCC‑GCATCAGGCGATGGTGCACTGAAGCCTGa                                                     <  1:5015409/93‑1 (MQ=25)
                     tatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGAt                                                    <  2:2542589/93‑1 (MQ=37)
                     tatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGAt                                                    >  1:304963/1‑93 (MQ=25)
                      atGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATg                                                   >  1:4671603/1‑93 (MQ=25)
                       tGGTGTATTGAGGGTTGCTCTGCCTGATGCCGATCCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                  <  2:1432397/93‑1 (MQ=21)
                       tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                  <  1:5912112/93‑1 (MQ=25)
                       tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                  <  2:6033665/93‑1 (MQ=25)
                        ggggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCg                                                 >  1:3696465/1‑93 (MQ=25)
                         gggTATTGAGGGTGGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGa                                                <  2:2744155/93‑1 (MQ=25)
                          ggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGAc                                               <  1:6025565/93‑1 (MQ=25)
                            tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGc                                             <  2:1889237/93‑1 (MQ=25)
                             aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCt                                            >  1:172718/1‑93 (MQ=25)
                             aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCt                                            <  2:1754872/93‑1 (MQ=25)
                              ttGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCtt                                           >  1:582904/1‑93 (MQ=25)
                              ttGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCtt                                           >  2:3131807/1‑93 (MQ=25)
                                gAGGTTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc                                         <  1:1398880/93‑1 (MQ=25)
                                gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc                                         >  2:4162660/1‑93 (MQ=25)
                                 aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                        >  1:4415003/1‑93 (MQ=25)
                                  ggtttGCTATGCCTGATGCCGATTGGTAGGCGGAAGAAGGCGCTCGCGCC‑GCACCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcg                                       <  1:5505612/90‑1 (MQ=21)
                                  gggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcg                                       >  2:2447480/1‑93 (MQ=25)
                                   ggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                      >  1:2564788/1‑93 (MQ=25)
                                   ggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                      <  1:889559/93‑1 (MQ=25)
                                     ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt                                    <  2:774639/93‑1 (MQ=25)
                                      tgatATGCCTGTTGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTc                                   <  1:695115/90‑1 (MQ=21)
                                      tGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTc                                   <  2:3726098/93‑1 (MQ=25)
                                       gCTATGCCTGATGCCGATTCGTAGGCTGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt                                  >  2:6394915/1‑93 (MQ=21)
                                         tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa                                >  2:5028851/1‑93 (MQ=25)
                                          aTGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTAt                               >  2:2005146/1‑93 (MQ=21)
                                           tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc                              >  1:222789/1‑93 (MQ=21)
                                            gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             >  2:3315186/1‑93 (MQ=21)
                                            gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             >  2:3584288/1‑93 (MQ=21)
                                              cTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg                           >  1:6092452/1‑93 (MQ=21)
                                              cTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg                           >  1:1318856/1‑93 (MQ=21)
                                              atGATGCCGATTCGTAGGCGGGATAGGGCGCTCGCG‑CGGCATCGGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg                           <  1:2483812/92‑1 (MQ=21)
                                               tGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc                          >  1:50743/1‑93 (MQ=21)
                                               ggATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc                          <  2:4857922/92‑1 (MQ=21)
                                                gATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑TCATCGGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGcc                         <  2:1499121/93‑1 (MQ=21)
                                                gATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGcc                         <  1:1022002/93‑1 (MQ=21)
                                                gATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGcc                         >  2:3022217/1‑93 (MQ=21)
                                                 aTGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCt                        >  1:6202518/1‑93 (MQ=21)
                                                 aTGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCt                        <  2:4664065/93‑1 (MQ=21)
                                                  tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa                       >  1:5626370/1‑93 (MQ=21)
                                                  tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa                       <  1:3652658/93‑1 (MQ=21)
                                                  tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa                       >  2:1281497/1‑93 (MQ=21)
                                                   gCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTAc                      <  2:487827/93‑1 (MQ=21)
                                                     cGATTCGTAGGCCGGATAGGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg                    <  1:3470401/93‑1 (MQ=21)
                                                     cGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg                    <  2:3752176/93‑1 (MQ=21)
                                                      gATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                   >  2:5143517/1‑93 (MQ=21)
                                                      gATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                   >  1:4888919/1‑93 (MQ=21)
                                                      gATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                   >  2:2173903/1‑93 (MQ=18)
                                                      gATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                   <  1:2731756/93‑1 (MQ=21)
                                                       aTTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                  >  1:4146983/1‑93 (MQ=18)
                                                       aTTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                  <  2:1699976/93‑1 (MQ=18)
                                                        ttCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGa                 >  1:513088/1‑93 (MQ=21)
                                                         tCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAg                <  2:911382/93‑1 (MQ=21)
                                                           gTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCa              >  1:2891166/1‑93 (MQ=21)
                                                           gTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCCACTGGGAgac              >  2:362933/1‑91 (MQ=18)
                                                            tAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAc             >  1:1236580/1‑93 (MQ=21)
                                                             aGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACg            >  2:4768402/1‑93 (MQ=21)
                                                             aGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACg            >  1:3186288/1‑93 (MQ=21)
                                                              ggCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGc           >  2:379782/1‑93 (MQ=21)
                                                              ggCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGc           >  2:1147612/1‑93 (MQ=21)
                                                                ccGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCtt         <  1:2405822/93‑1 (MQ=21)
                                                                 cGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCttt        >  1:1894725/1‑93 (MQ=21)
                                                                    aTAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTaca     >  1:938210/1‑93 (MQ=25)
                                                                     tAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTacac    >  2:3197327/1‑93 (MQ=25)
                                                                     tAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTacac    <  1:1266728/93‑1 (MQ=25)
                                                                      aaGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACAcc   <  1:4344468/93‑1 (MQ=25)
                                                                       aGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCg  >  1:402262/1‑93 (MQ=25)
                                                                                  |                                                                                  
AGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCG‑G‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG  >  NC_000913/4448462‑4448623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑