BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,528	T→C	Q8R (CAG→CGG)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,528	0	T	C	100.0%	717.8 / NA	207	Q8R (CAG→CGG)	araD	L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (100/107); total (100/107)

Only 100 of 210 total aligned reads displayed.

ACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGAT  >  NC_000913/66438‑66617

aCGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCgg                                                                                         <  2:6080340/93‑1 (MQ=255)

aCGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCgg                                                                                         <  1:747741/93‑1 (MQ=255)

     gcgcTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGccc                                                                                    >  1:3355885‑M1/1‑90 (MQ=255)

     gcgcTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGccc                                                                                    >  1:2807122‑M1/1‑90 (MQ=255)

     gcgcTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGccc                                                                                    >  2:1498812‑M1/1‑90 (MQ=255)

      cgcTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccg                                                                                   >  2:1648188‑M1/1‑89 (MQ=255)

      cgcTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccg                                                                                   <  2:1133374‑M1/93‑5 (MQ=255)

       gcTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccga                                                                                  <  1:5916515‑M1/93‑6 (MQ=255)

         tCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgaga                                                                                <  2:5598764‑M1/93‑8 (MQ=255)

          cgcgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagac                                                                               <  1:4484797‑M1/93‑9 (MQ=255)

           gcgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacc                                                                              >  2:712069‑M1/1‑84 (MQ=255)

            cgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagaccc                                                                             >  2:5738424‑M1/1‑83 (MQ=255)

             gATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccg                                                                            >  1:6377529‑M1/1‑82 (MQ=255)

              aTCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgc                                                                           >  2:1471128‑M1/1‑81 (MQ=255)

              aTCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgc                                                                           >  1:391317‑M1/1‑81 (MQ=255)

              aTCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgc                                                                           >  1:904825‑M1/1‑81 (MQ=255)

               tCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgca                                                                          <  1:2291026‑M1/93‑14 (MQ=255)

               tCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgca                                                                          <  2:279410‑M1/93‑14 (MQ=255)

               tCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgca                                                                          >  1:3640363‑M1/1‑80 (MQ=255)

               tCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgca                                                                          >  2:3865017‑M1/1‑80 (MQ=255)

                cAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcac                                                                         >  1:5040093‑M1/1‑79 (MQ=255)

                cAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcac                                                                         <  1:4332114‑M1/93‑15 (MQ=255)

                  aCGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgc                                                                       >  2:5048556‑M1/1‑77 (MQ=255)

                   cGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcg                                                                      >  2:1921739‑M1/1‑76 (MQ=255)

                   cGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcg                                                                      >  1:4511987‑M1/1‑76 (MQ=255)

                    ggCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgt                                                                     >  2:2173642‑M1/1‑75 (MQ=255)

                         tgccgttGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacc                                                                <  1:1256940‑M1/90‑24 (MQ=255)

                         tGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacc                                                                <  2:2376207‑M1/93‑24 (MQ=255)

                         tGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacc                                                                <  2:1981275‑M1/93‑24 (MQ=255)

                          gACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagactcgcacgcgtcgacct                                                               >  1:5199407‑M1/1‑69 (MQ=255)

                           aCGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctg                                                              <  2:896534‑M1/93‑26 (MQ=255)

                           aCGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctg                                                              <  1:1428835‑M1/93‑26 (MQ=255)

                             gTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGGCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgca                                                            <  2:4868672‑M1/93‑28 (MQ=255)

                              ttGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcag                                                           >  2:44667‑M1/1‑65 (MQ=255)

                               tGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcagg                                                          >  1:2046422‑M1/1‑64 (MQ=255)

                                gCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggt                                                         <  2:5119137‑M1/93‑31 (MQ=255)

                                gCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggt                                                         <  1:1952949‑M1/93‑31 (MQ=255)

                                gCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggt                                                         >  1:250832‑M1/1‑63 (MQ=255)

                                 ccccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggta                                                        <  1:4347190‑M1/93‑32 (MQ=255)

                                  cccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtat                                                       <  2:4322777‑M1/93‑33 (MQ=255)

                                   ccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatg                                                      >  2:5805128‑M1/1‑60 (MQ=255)

                                     aTGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatgga                                                    >  2:2577515‑M1/1‑58 (MQ=255)

                                       gtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggaga                                                  >  2:1167741‑M1/1‑56 (MQ=255)

                                       gtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggaga                                                  >  2:3110792‑M1/1‑56 (MQ=255)

                                         gAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaa                                                <  1:3290517‑M1/93‑40 (MQ=255)

                                         gAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaa                                                >  2:2621924‑M1/1‑54 (MQ=255)

                                          aGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaac                                               <  1:560290‑M1/93‑41 (MQ=255)

                                           gCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaaca                                              >  1:1501338‑M1/1‑52 (MQ=255)

                                           gCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaaca                                              <  1:1762987‑M1/93‑42 (MQ=255)

                                            gcttaccagGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacag                                             <  2:6081195‑M1/89‑43 (MQ=255)

                                            cGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacag                                             <  1:636774‑M1/93‑43 (MQ=255)

                                              tGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagta                                           <  1:3163306‑M1/93‑45 (MQ=255)

                                               gACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtag                                          >  2:1245771‑M1/1‑48 (MQ=255)

                                                aCCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtaga                                         >  2:1294063‑M1/1‑47 (MQ=255)

                                                  cAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGgccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagaga                                       >  2:3987199‑M1/1‑45 (MQ=255)

                                                    ggTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagt                                     >  1:1317214‑M1/1‑43 (MQ=255)

                                                    ggTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagt                                     <  1:836948‑M1/93‑51 (MQ=255)

                                                     gTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagtt                                    <  1:5255619‑M1/93‑52 (MQ=255)

                                                      gtgtgtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttg                                   <  2:652507‑M1/92‑53 (MQ=255)

                                                           ttttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgata                              >  2:3300381‑M1/1‑36 (MQ=255)

                                                            tttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataa                             <  2:853737‑M1/93‑59 (MQ=255)

                                                             ttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaa                            >  2:6342733‑M1/1‑34 (MQ=255)

                                                               ggCAGCGCCAGGTTGTCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaa                          <  1:2595150‑M1/93‑62 (MQ=255)

                                                               ggCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaa                          >  2:5905226‑M1/1‑32 (MQ=255)

                                                               ggCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaa                          <  2:5249910‑M1/93‑62 (MQ=255)

                                                               ggCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggcatggagaaacagtagagagttgcggtaaaaa                          >  2:2760161‑M1/1‑32 (MQ=255)

                                                                gCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaag                         <  2:4638398‑M1/93‑63 (MQ=255)

                                                                 cAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagc                        <  1:4869329‑M1/93‑64 (MQ=255)

                                                                 cAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagc                        <  1:6085028‑M1/93‑64 (MQ=255)

                                                                   gcgcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgt                      <  1:2382591‑M1/93‑66 (MQ=255)

                                                                   gcgcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgt                      <  2:4427269‑M1/93‑66 (MQ=255)

                                                                   gcgcCAGGTTGGCTTCTAATACCCGGCGccagagacccgcacgcgtcgacgtgcaggtatggagaaacagtagagagttgcgataaaaagcgt                      <  1:6274370‑M1/93‑66 (MQ=255)

                                                                     gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtca                    >  2:543285‑M1/1‑26 (MQ=255)

                                                                     gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtca                    >  1:2658986‑M1/1‑26 (MQ=255)

                                                                     gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtca                    <  2:3691313‑M1/93‑68 (MQ=255)

                                                                     gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtca                    <  2:2256316‑M1/93‑68 (MQ=255)

                                                                      ccAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcag                   <  2:6355237‑M1/93‑69 (MQ=255)

                                                                       cAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcagg                  >  1:292034‑M1/1‑24 (MQ=255)

                                                                       cAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcagg                  >  1:2418246‑M1/1‑24 (MQ=255)

                                                                        aGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggt                 <  1:5708059‑M1/93‑71 (MQ=255)

                                                                          gTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtag               >  1:2081507‑M1/1‑21 (MQ=255)

                                                                            tGGCTTCTAATCCCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtagga             <  1:3856585‑M1/93‑75 (MQ=255)

                                                                            tGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtagga             <  1:2737976‑M1/93‑75 (MQ=255)

                                                                             ggCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggat            >  1:4379794‑M1/1‑18 (MQ=255)

                                                                              gCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatc           <  1:2965318‑M1/93‑77 (MQ=255)

                                                                              gCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatc           <  2:5295265‑M1/93‑77 (MQ=255)

                                                                              gCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatc           <  1:2552558‑M1/93‑77 (MQ=255)

                                                                              gCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatc           <  1:5266174‑M1/93‑77 (MQ=255)

                                                                                ttCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccg         >  1:5757823‑M1/1‑15 (MQ=255)

                                                                                  cTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgct       <  2:2715631‑M1/93‑81 (MQ=255)

                                                                                   tAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgcta      >  1:4158448‑M1/1‑12 (MQ=255)

                                                                                   tAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgcta      >  1:4691629‑M1/1‑12 (MQ=255)

                                                                                   tAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgcta      >  1:4986925‑M1/1‑12 (MQ=255)

                                                                                   tAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgcta      >  2:2857704‑M1/1‑12 (MQ=255)

                                                                                    aaTACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaa     <  2:4097980‑M1/93‑83 (MQ=255)

                                                                                    aaTACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaa     <  2:4787062‑M1/93‑83 (MQ=255)

                                                                                     aTACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaat    >  2:1794799‑M1/1‑10 (MQ=255)

                                                                                      tACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatc   >  1:1000921‑M1/1‑9 (MQ=255)

                                                                                       aCCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatct  >  2:3599865‑M1/1‑8 (MQ=255)

                                                                                       aCCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatct  <  2:372649‑M1/93‑86 (MQ=255)

ACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGAT  >  NC_000913/66438‑66617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`