BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,061,451	A→G	T144A (ACC→GCC)	torD →	TorD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,061,451	0	A	G	99.6%	890.2 / NA	262	T144A (ACC→GCC)	torD	TorD
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (133/128); minor base T (0/1); total (133/129)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

GAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGT  >  NC_000913/1061361‑1061540

   aCCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGtt                                                                                      >  2:2650707/1‑93 (MQ=255)

   aCCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGtt                                                                                      >  1:5645114/1‑93 (MQ=255)

     cAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTcc                                                                                    >  2:922837/1‑93 (MQ=255)

     cAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTcc                                                                                    >  2:1793054/1‑93 (MQ=255)

     cAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTcc                                                                                    >  2:875010/1‑93 (MQ=255)

      aGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCt                                                                                   <  1:2269535/93‑1 (MQ=255)

      aGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCt                                                                                   >  2:2449514/1‑93 (MQ=255)

       gCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTg                                                                                  >  1:4051765/1‑93 (MQ=255)

         ggCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTgcg                                                                                <  1:3135292/93‑1 (MQ=255)

         ggCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTgcg                                                                                <  1:5128251/93‑1 (MQ=255)

          gCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTgcgc                                                                               <  2:5983432/93‑1 (MQ=255)

             aTTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCgaa                                                                            <  2:2013403/93‑1 (MQ=255)

               tgcAACGAACCGGCATATCATCGGGCGATCGATCTCGAATTGCTCAGCCATCTGCATTTTTCGCAGGGAGAGGGGGCCGTTCCTGCGCgaaga                                                                          <  1:4780935/91‑1 (MQ=255)

                 cAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAAt                                                                        <  1:976289/93‑1 (MQ=255)

                 cAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAAt                                                                        <  2:723537/93‑1 (MQ=255)

                  aaCGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATc                                                                       <  2:3421568/93‑1 (MQ=255)

                   aCGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCg                                                                      >  2:2571026/1‑93 (MQ=255)

                    cGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGa                                                                     <  2:3319056/93‑1 (MQ=255)

                    cGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGa                                                                     <  1:1528930/93‑1 (MQ=255)

                     gAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGAc                                                                    <  1:1951670/93‑1 (MQ=255)

                      aaCCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACa                                                                   <  1:1287259/93‑1 (MQ=255)

                      aaCCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACa                                                                   >  2:4739149/1‑93 (MQ=255)

                       aCCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAg                                                                  >  1:1169260/1‑93 (MQ=255)

                        ccGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGt                                                                 <  1:3258119/93‑1 (MQ=255)

                        ccGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGt                                                                 >  2:4458825/1‑93 (MQ=255)

                         cGGCAGATCATCTGGGGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGtt                                                                <  1:6201555/93‑1 (MQ=255)

                         cGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGtt                                                                <  2:4778798/93‑1 (MQ=255)

                         cGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGtt                                                                <  2:5363522/93‑1 (MQ=255)

                          ggCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGttt                                                               >  1:3362875/1‑93 (MQ=255)

                          ggCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGttt                                                               <  2:1313166/93‑1 (MQ=255)

                          ggCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGttt                                                               <  2:1194834/93‑1 (MQ=255)

                           gCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTg                                                              >  1:6445498/1‑93 (MQ=255)

                             aGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCg                                                            >  2:4618838/1‑93 (MQ=255)

                             aGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCg                                                            <  1:5291733/93‑1 (MQ=255)

                              gATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCgg                                                           <  1:3485295/93‑1 (MQ=255)

                              gATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCgg                                                           >  2:357411/1‑93 (MQ=255)

                               atcatcTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCCCGAAGAATCGACAGCTTGCGGc                                                          >  1:1881661/1‑93 (MQ=255)

                                tcatcTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCa                                                         <  1:4987859/93‑1 (MQ=255)

                                tcatcTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCa                                                         <  1:1638852/93‑1 (MQ=255)

                                 catcTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCaa                                                        >  2:5725963/1‑93 (MQ=255)

                                   tcTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGGTTGCGGCaaaa                                                      >  1:2941727/1‑93 (MQ=255)

                                     tGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCaaaaaa                                                    >  1:5540511/1‑93 (MQ=255)

                                      ggCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAac                                                   >  2:1534859/1‑93 (MQ=255)

                                        ccatctatcaCGAATTGCTCAGCCATATGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAacac                                                 <  1:4556474/91‑1 (MQ=255)

                                           tctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGCGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGa                                              >  2:3047312/1‑93 (MQ=255)

                                           tctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGa                                              <  2:4215631/93‑1 (MQ=255)

                                           tctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGa                                              >  1:5771460/1‑93 (MQ=255)

                                           tctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGa                                              >  1:2373557/1‑93 (MQ=255)

                                           tctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGa                                              >  2:5918792/1‑93 (MQ=255)

                                           tctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGa                                              <  2:5877846/93‑1 (MQ=255)

                                            ctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGAc                                             <  1:846342/93‑1 (MQ=255)

                                            ctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGAc                                             <  1:4847000/93‑1 (MQ=255)

                                            ctatctCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGAc                                             <  1:2061146/93‑1 (MQ=255)

                                               tctcGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGgc                                          <  1:6001888/93‑1 (MQ=255)

                                               tctcGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGgc                                          >  2:4414032/1‑93 (MQ=255)

                                               tctcGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGgc                                          >  1:4186769/1‑93 (MQ=255)

                                                 tcGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGgcgc                                        >  2:2947141/1‑93 (MQ=255)

                                                 tcGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGgcgc                                        >  2:4318386/1‑93 (MQ=255)

                                                    aaTTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCtgt                                     <  2:3252280/93‑1 (MQ=255)

                                                      ttGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTgg                                   >  2:2220764/1‑93 (MQ=255)

                                                       tGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGc                                  <  2:5061492/93‑1 (MQ=255)

                                                        gCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCa                                 <  1:4502841/93‑1 (MQ=255)

                                                         cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCaa                                <  1:1101532/93‑1 (MQ=255)

                                                          tCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAAt                               <  2:4922461/93‑1 (MQ=255)

                                                           cAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATg                              <  2:1132138/93‑1 (MQ=255)

                                                           cAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATg                              >  2:3580426/1‑93 (MQ=255)

                                                           cAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATg                              <  1:3278386/93‑1 (MQ=255)

                                                            aGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATgg                             >  1:1756613/1‑93 (MQ=255)

                                                            aGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATgg                             >  2:5489474/1‑93 (MQ=255)

                                                            aGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATgg                             >  1:781595/1‑93 (MQ=255)

                                                             gCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGt                            >  2:5004619/1‑93 (MQ=255)

                                                             gCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGt                            >  2:3955233/1‑93 (MQ=255)

                                                              cgaTCTGCATTTTTCGCTGGGAGAGAGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAGAACACTGACGGCGCTGTGGCAATGGtt                           <  1:1272718/91‑1 (MQ=255)

                                                              ccATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGtt                           >  1:2558166/1‑93 (MQ=255)

                                                              ccATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGtt                           <  1:354443/93‑1 (MQ=255)

                                                              ccATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGACAAAAAACACTGACGGCGCTGTGGCAATGGtt                           <  1:1076747/93‑1 (MQ=255)

                                                               cATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTa                          >  2:4128374/1‑93 (MQ=255)

                                                                 tCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTAcc                        >  2:1049719/1‑93 (MQ=255)

                                                                   tGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCag                      >  1:5890985/1‑93 (MQ=255)

                                                                    gCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCaga                     >  2:605321/1‑93 (MQ=255)

                                                                     cATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCagag                    <  1:280227/93‑1 (MQ=255)

                                                                      aTTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGt                   <  1:6116735/93‑1 (MQ=255)

                                                                       tttttCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGtt                  <  2:1524592/93‑1 (MQ=255)

                                                                        ttttCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGttt                 <  1:4911502/93‑1 (MQ=255)

                                                                          ttCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTttgt               <  2:5380715/93‑1 (MQ=255)

                                                                           tCGCTGGGAGAGGTGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTttgtt              <  1:2432683/93‑1 (MQ=255)

                                                                             gCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTgc            >  1:3725025/1‑93 (MQ=255)

                                                                              cTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTgcg           >  1:265430/1‑93 (MQ=255)

                                                                                gggAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGcgcg         >  2:1895712/1‑93 (MQ=255)

                                                                                 ggAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGt        >  2:3290122/1‑93 (MQ=255)

                                                                                 ggAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGt        <  2:6250696/93‑1 (MQ=255)

                                                                                 ggAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGt        <  1:4376965/93‑1 (MQ=255)

                                                                                  gagaGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGtt       >  2:6444077/1‑93 (MQ=255)

                                                                                   agagGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTg      <  1:3043095/93‑1 (MQ=255)

                                                                                   agagGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTg      >  2:2743734/1‑93 (MQ=255)

                                                                                    gagGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTgt     <  1:2120076/93‑1 (MQ=255)

                                                                                    gagGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTgt     <  2:3747995/93‑1 (MQ=255)

                                                                                    gagGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTgt     <  2:6169320/93‑1 (MQ=255)

                                                                                      ggggGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTgtcg   >  2:2872884/1‑93 (MQ=255)

                                                                                       ggggCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTgtcgt  >  2:4025301/1‑93 (MQ=255)

GAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGT  >  NC_000913/1061361‑1061540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`