BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,017	T→C	F404L (TTT→CTT)	agp →	periplasmic glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,017	0	T	C	100.0%	718.1 / NA	202	F404L (TTT→CTT)	agp	periplasmic glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (91/111); total (91/111)

Only 100 of 211 total aligned reads displayed.

GCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCG  >  NC_000913/1065927‑1066105

 cGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTg                                                                                       <  2:6062743/93‑1 (MQ=255)

  gTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGa                                                                                      >  2:4287453/1‑93 (MQ=255)

   ttAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGAt                                                                                     >  2:1310837/1‑93 (MQ=255)

   ttAACCCTGCAGGCACCTGCACAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGAt                                                                                     <  2:2752474/93‑1 (MQ=255)

    tAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATa                                                                                    <  1:5907629/93‑1 (MQ=255)

     aaCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAg                                                                                   >  1:4751096/1‑93 (MQ=255)

     aaCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAg                                                                                   >  2:2483786/1‑93 (MQ=255)

     aaCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAg                                                                                   >  1:5864401/1‑93 (MQ=255)

      aCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGc                                                                                  <  2:1527843/93‑1 (MQ=255)

      aCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGc                                                                                  <  2:6256461/93‑1 (MQ=255)

        ccTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCgt                                                                                <  2:2616667/93‑1 (MQ=255)

          tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCgtgt                                                                              >  2:4352293/1‑93 (MQ=255)

           gCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGtt                                                                             <  1:4394991/93‑1 (MQ=255)

           gCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGtt                                                                             <  2:3117811/93‑1 (MQ=255)

            cAGGCACCTGCGCAGCGTGTGACGCTTGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTtg                                                                            <  2:2032610/93‑1 (MQ=255)

               gCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTtgaat                                                                         >  1:4076300/1‑93 (MQ=255)

               gCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTtgaat                                                                         >  2:6462344/1‑93 (MQ=255)

                cACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTtgaatg                                                                        <  1:678283/93‑1 (MQ=255)

                 aCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTtgaatga                                                                       >  1:1474738/1‑93 (MQ=255)

                   cTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAg                                                                     <  2:3818086/93‑1 (MQ=255)

                   cTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGGTTCTGCCCCATGGGTAAGCTTGATAGCGTGTttaatgaaa                                                                     >  1:4682408/1‑92 (MQ=255)

                    tGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGc                                                                    <  1:5002394/93‑1 (MQ=255)

                    tGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGc                                                                    <  2:5556846/93‑1 (MQ=255)

                      cgcAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCgg                                                                  >  1:2350545/1‑93 (MQ=255)

                       gcAGCGTGTGACGCTGGAATTAAGCTGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCgct                                                                 <  2:6245545/93‑3 (MQ=255)

                         aGCGTGTGGCGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGa                                                               <  1:4902317/93‑1 (MQ=255)

                         aGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGa                                                               >  1:1728119/1‑93 (MQ=255)

                         aGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGa                                                               >  2:228362/1‑93 (MQ=255)

                         aGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGa                                                               <  2:5035443/93‑1 (MQ=255)

                          gCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGaa                                                              >  1:1771724/1‑93 (MQ=255)

                          gCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGaa                                                              >  2:5420924/1‑93 (MQ=255)

                             tgtgACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATa                                                           >  1:5391385/1‑93 (MQ=255)

                             tgtgACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATa                                                           >  2:3339342/1‑93 (MQ=255)

                              gtgACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATaa                                                          >  2:917647/1‑93 (MQ=255)

                               tgACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAAc                                                         <  1:1315280/93‑1 (MQ=255)

                                gACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATaaaa                                                        <  1:6178776/93‑3 (MQ=255)

                                gACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACa                                                        <  2:5756450/93‑1 (MQ=255)

                                gACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACa                                                        >  2:4586156/1‑93 (MQ=255)

                                gACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACa                                                        >  2:2999487/1‑93 (MQ=255)

                                gACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACa                                                        <  1:62385/93‑1 (MQ=255)

                                      ggAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAATCGGTGAAATATCAGAAAAc                                                  <  1:6277342/93‑1 (MQ=255)

                                      ggAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAAc                                                  <  1:2626895/93‑1 (MQ=255)

                                      ggAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAAc                                                  >  2:3494197/1‑93 (MQ=255)

                                       gAATTAAGCGGGTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACt                                                 <  2:2190719/93‑1 (MQ=255)

                                        aaTTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTc                                                >  2:5685322/1‑93 (MQ=255)

                                         aTTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcc                                               >  1:1940668/1‑93 (MQ=255)

                                         aTTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcc                                               <  1:5541344/93‑1 (MQ=255)

                                          ttAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTccc                                              <  1:6332328/93‑1 (MQ=255)

                                           tAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcccc                                             <  1:1050280/93‑1 (MQ=255)

                                           tAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcccc                                             <  2:3731409/93‑1 (MQ=255)

                                           tAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcccc                                             <  1:4252625/93‑1 (MQ=255)

                                            aaGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTccccc                                            <  1:3628060/93‑1 (MQ=255)

                                              gCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCcgc                                          <  2:2931979/93‑1 (MQ=255)

                                              gCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCcgc                                          <  2:169395/93‑1 (MQ=255)

                                                 gTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgag                                       >  1:3701073/1‑93 (MQ=255)

                                                   tGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGaa                                     >  2:2803036/1‑93 (MQ=255)

                                                     cccGATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGc                                   <  2:302861/93‑1 (MQ=255)

                                                        gATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCggg                                <  2:5212332/93‑1 (MQ=255)

                                                        gATAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCggg                                <  1:1449132/93‑1 (MQ=255)

                                                         aTAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCgggg                               >  2:2657340/1‑93 (MQ=255)

                                                          tAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCggggg                              <  1:3831022/93‑1 (MQ=255)

                                                          tAGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCggggg                              >  1:3914727/1‑93 (MQ=255)

                                                           aGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGa                             >  2:1809204/1‑93 (MQ=255)

                                                           aGACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGa                             >  1:3184904/1‑93 (MQ=255)

                                                            gACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAg                            <  2:2173291/93‑1 (MQ=255)

                                                            gACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAg                            >  1:5175279/1‑93 (MQ=255)

                                                            gACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAg                            >  1:1630635/1‑93 (MQ=255)

                                                            gACGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAg                            <  1:3279373/93‑1 (MQ=255)

                                                             aCGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGt                           <  1:4444619/93‑1 (MQ=255)

                                                             aCGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGt                           >  1:873463/1‑93 (MQ=255)

                                                             aCGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCCCGAGGAGCgggggggg                           >  1:45921/1‑90 (MQ=255)

                                                              cGCTGATGTTTTCTGCCGGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTAAAATAACAGAAACCTCCCCCGCGAGAAGCGGGGGAGTc                          <  1:4027959/93‑1 (MQ=255)

                                                              cGCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTc                          >  2:80018/1‑93 (MQ=255)

                                                               gCTGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCg                         >  1:116607/1‑93 (MQ=255)

                                                               gCTGATGGTTTCTACCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCg                         <  2:6357188/93‑1 (MQ=255)

                                                                 tGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCt                       >  1:3420790/1‑93 (MQ=255)

                                                                 tGATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGGCGCt                       >  1:2700190/1‑93 (MQ=255)

                                                                  gATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTg                      >  2:4132470/1‑93 (MQ=255)

                                                                  gATGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTg                      >  1:3034896/1‑93 (MQ=255)

                                                                   aTGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTgg                     >  1:4174544/1‑93 (MQ=255)

                                                                    tGGTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGt                    <  1:3212540/93‑1 (MQ=255)

                                                                     ggTTTCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGtt                   <  1:3963980/93‑1 (MQ=255)

                                                                         tCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGCAATAACAGAAAACTTCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAAc               >  2:1919276/1‑93 (MQ=255)

                                                                         tCTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGGTaaag               >  1:2056921/1‑92 (MQ=255)

                                                                          cTGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAACCTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACg              <  1:510372/93‑1 (MQ=255)

                                                                           tGCCCGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGt             <  2:4307382/93‑1 (MQ=255)

                                                                             cccGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGttt           <  1:6220797/93‑1 (MQ=255)

                                                                              ccGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGtttt          >  1:5295841/1‑93 (MQ=255)

                                                                               cGATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGttttt         >  2:5955508/1‑93 (MQ=255)

                                                                                gATGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTa        <  1:5339898/93‑1 (MQ=255)

                                                                                 aTGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAACCTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTAc       <  2:5640435/93‑1 (MQ=255)

                                                                                 aTGGATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTAc       <  1:3747030/93‑1 (MQ=255)

                                                                                   ggATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAACCTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGt     <  2:31555/93‑1 (MQ=255)

                                                                                    gATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAACCACCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGtt    <  2:392235/93‑1 (MQ=255)

                                                                                    gATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGtt    >  1:5639731/1‑93 (MQ=255)

                                                                                    gATAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGtt    >  2:2094186/1‑93 (MQ=255)

                                                                                     aTAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTc   >  1:5125000/1‑93 (MQ=255)

                                                                                     aTAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTc   >  2:5894689/1‑93 (MQ=255)

                                                                                      tAAGCTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCg  >  2:4748456/1‑93 (MQ=255)

                                                                                      aaaGCTTGATAGTGTGTTGAATGAGGCGAAAAAAAAACAAAAACCCCCCCCGGGAGAGGGGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCg  <  2:1169834/92‑1 (MQ=255)

GCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCG  >  NC_000913/1065927‑1066105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`