BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,180	A→G	D120G (GAT→GGT)	ycfJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,180	0	A	G	99.5%	740.4 / ‑4.8	213	D120G (GAT→GGT)	ycfJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (96/116); total (96/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 217 total aligned reads displayed.

TGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGA  >  NC_000913/1167091‑1167270

tggtCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTAc                                                                                         <  2:1543495/93‑1 (MQ=255)

  gtCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACtt                                                                                       >  2:3722239/1‑93 (MQ=255)

   tCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTa                                                                                      <  1:911348/93‑1 (MQ=255)

    cgcgGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTac                                                                                     <  2:5996906/93‑1 (MQ=255)

     gcgGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTaca                                                                                    <  2:5265915/93‑1 (MQ=255)

     gcgGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTaca                                                                                    >  2:2233533/1‑93 (MQ=255)

      cgGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTacac                                                                                   <  2:1357311/93‑1 (MQ=255)

       ggTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTGTCTCCAGGAAAGCGGTACTTACACg                                                                                  >  2:3767539/1‑93 (MQ=255)

         tAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGAc                                                                                >  1:4608671/1‑93 (MQ=255)

           aaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTa                                                                              >  2:1438779/1‑93 (MQ=255)

           aaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTa                                                                              <  2:4484774/93‑1 (MQ=255)

           aaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTa                                                                              <  2:4460085/93‑1 (MQ=255)

           aaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTa                                                                              >  2:382790/1‑93 (MQ=255)

            aGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTAc                                                                             >  1:3468300/1‑93 (MQ=255)

            aGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTAc                                                                             >  1:384731/1‑93 (MQ=255)

             gATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACg                                                                            <  2:2030830/93‑1 (MQ=255)

                gTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCaa                                                                         <  2:4849116/93‑1 (MQ=255)

                 tCGCCACTTGTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAAc                                                                        <  2:5047592/93‑1 (MQ=255)

                 tCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAAc                                                                        <  2:3525449/93‑1 (MQ=255)

                   gCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTAAGCAACAg                                                                      >  2:2602354/1‑93 (MQ=255)

                   gCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGATAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAg                                                                      <  2:3029096/93‑1 (MQ=255)

                     cACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCg                                                                    >  1:1787874/1‑93 (MQ=255)

                      aCTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGt                                                                   <  2:3035154/93‑1 (MQ=255)

                      aCTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGt                                                                   <  1:2013148/93‑1 (MQ=255)

                      aCTGTGGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGt                                                                   <  2:5548401/93‑1 (MQ=255)

                       cTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCTAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGtt                                                                  <  1:125020/93‑1 (MQ=255)

                       cTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGtt                                                                  >  2:4697255/1‑93 (MQ=255)

                       cTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGtt                                                                  <  2:449953/93‑1 (MQ=255)

                       cTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGtt                                                                  <  2:4060321/93‑1 (MQ=255)

                         gttgtGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGt                                                                <  1:272114/93‑1 (MQ=255)

                          ttgtGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTa                                                               <  1:1626040/93‑1 (MQ=255)

                             tgGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTaaaa                                                            <  1:85443/93‑1 (MQ=255)

                              gggggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAAc                                                           <  2:2159995/93‑1 (MQ=255)

                               ggggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACg                                                          <  2:5448130/93‑1 (MQ=255)

                               ggggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACg                                                          >  2:115088/1‑93 (MQ=255)

                                gggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACgg                                                         <  2:460686/93‑1 (MQ=255)

                                 ggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGgt                                                        <  2:5855721/93‑1 (MQ=255)

                                  gcgcTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGgtg                                                       <  2:3266728/93‑1 (MQ=255)

                                  gcgcTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGgtg                                                       >  2:1760037/1‑93 (MQ=255)

                                  gcgcTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGgtg                                                       <  2:145229/93‑1 (MQ=255)

                                      tGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCGAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATg                                                   <  1:5788872/93‑1 (MQ=255)

                                       gggTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGa                                                  >  1:1374778/1‑93 (MQ=255)

                                       gggTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGa                                                  >  1:3610472/1‑93 (MQ=255)

                                        ggtggtGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGAc                                                 <  1:2123708/93‑1 (MQ=255)

                                          tggtggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACaa                                               >  2:6283187/1‑93 (MQ=255)

                                          tggtggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACaa                                               <  2:2375517/93‑1 (MQ=255)

                                            gtggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGt                                             <  2:3341573/93‑1 (MQ=255)

                                              ggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCa                                           <  2:1955427/93‑1 (MQ=255)

                                                 tatGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaa                                        <  1:4734610/93‑1 (MQ=255)

                                                 tatGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaa                                        <  2:3471393/93‑1 (MQ=255)

                                                   tGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaaaa                                      >  2:4807500/1‑93 (MQ=255)

                                                    gCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaaaaa                                     >  1:330789/1‑93 (MQ=255)

                                                     ccGGTAACGAGATCCAGGGCTATCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaaaaaa                                    <  1:5518827/93‑1 (MQ=255)

                                                      cGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAAt                                   <  2:4118983/93‑1 (MQ=255)

                                                       ggTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATg                                  <  2:2988869/93‑1 (MQ=255)

                                                       cgTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATg                                  <  2:5301125/92‑1 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 <  2:3351794/93‑1 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 <  1:2244972/93‑1 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 <  1:664882/93‑1 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 >  2:5827847/1‑93 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 <  2:1595012/93‑1 (MQ=255)

                                                         tAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGCGTATGACAAGTCAGAAAAAAAGCt                                >  1:5462756/1‑93 (MQ=255)

                                                          aaCCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTc                               >  2:1213210/1‑93 (MQ=255)

                                                          aaCCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTc                               >  2:3388205/1‑93 (MQ=255)

                                                           aCCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCg                              <  2:676121/93‑1 (MQ=255)

                                                            ccAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCgg                             >  2:5658981/1‑93 (MQ=255)

                                                            ccAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCgg                             >  1:263330/1‑93 (MQ=255)

                                                             cAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGt                            >  1:4402379/1‑93 (MQ=255)

                                                             cAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGt                            <  2:607784/93‑1 (MQ=255)

                                                               gATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTa                          >  2:3650182/1‑93 (MQ=255)

                                                                aTCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTat                         <  2:2403309/93‑1 (MQ=255)

                                                                 tCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatg                        <  2:342335/93‑1 (MQ=255)

                                                                  ccAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatga                       >  1:2553729/1‑93 (MQ=255)

                                                                   cAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatgat                      <  2:1299829/93‑1 (MQ=255)

                                                                    aGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatgatg                     >  1:5037746/1‑93 (MQ=255)

                                                                    aGGGCGCTATCCAGGAAAGCGGTACTTGCGCGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatgatg                     <  1:1936562/93‑1 (MQ=255)

                                                                     gggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAATTCAGAAAAAATGCTCGGTTATGAtgt                    >  1:5202812/1‑93 (MQ=255)

                                                                     gggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGAtgt                    <  2:5410938/93‑1 (MQ=255)

                                                                     gggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGAtgt                    <  2:226177/93‑1 (MQ=255)

                                                                      ggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGAtgtg                   >  1:5423058/1‑93 (MQ=255)

                                                                      ggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGAtgtg                   >  1:1575480/1‑93 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                <  2:5904389/93‑1 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                >  2:75896/1‑93 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                >  1:204218/1‑93 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                <  1:294223/93‑1 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                <  1:3409176/93‑1 (MQ=255)

                                                                          ctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCt               >  1:1921838/1‑93 (MQ=255)

                                                                          ctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCt               >  1:5235776/1‑93 (MQ=255)

                                                                             tcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCtata            <  2:1712952/93‑1 (MQ=255)

                                                                               cAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAg          <  2:5783953/93‑1 (MQ=255)

                                                                               cAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAg          <  1:169656/93‑1 (MQ=255)

                                                                               cAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAg          >  1:4344340/1‑93 (MQ=255)

                                                                               cAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAg          >  1:5063305/1‑93 (MQ=255)

                                                                                  gAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGAtt       <  1:3185150/93‑1 (MQ=255)

                                                                                   aaaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTg      <  1:3357499/93‑1 (MQ=255)

                                                                                   aaaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTg      >  1:5339187/1‑93 (MQ=255)

                                                                                   aaaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTg      <  1:1436838/93‑1 (MQ=255)

                                                                                    aaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTgg     >  2:2809841/1‑93 (MQ=255)

                                                                                    aaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTgg     >  1:6309977/1‑93 (MQ=255)

                                                                                      gCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCg   <  1:5932034/93‑1 (MQ=255)

TGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGA  >  NC_000913/1167091‑1167270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`