BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	231,829	T→C	G236G (GGT→GGC)	yafD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	231,829	0	T	C	99.5%	707.4 / NA	206	G236G (GGT→GGC)	yafD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (89/115); minor base G (1/0); total (91/115)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

GCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAA  >  NC_000913/231739‑231918

    gAAGTGTCGCTGCGCCAGGTGGGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAAc                                                                                     <  1:228461/93‑1 (MQ=255)

    gAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAAc                                                                                     <  1:2467080/93‑1 (MQ=255)

    gAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAAc                                                                                     <  1:4609658/93‑1 (MQ=255)

      aaTGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGt                                                                                   <  1:2618760/93‑1 (MQ=255)

      aaTGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGGTTGGTCGCCCGCTCGATTTTGTTTTTTACCGTGGCCTGAACGt                                                                                   >  1:20402/1‑93 (MQ=255)

         gTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAg                                                                                >  1:1470067/1‑93 (MQ=255)

           cGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTg                                                                              >  2:705104/1‑93 (MQ=255)

             cTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGaa                                                                            >  1:6323623/1‑93 (MQ=255)

              tGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAg                                                                           >  2:4843234/1‑93 (MQ=255)

              tGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAg                                                                           >  2:5291132/1‑93 (MQ=255)

               gcgcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGc                                                                          <  1:874599/93‑1 (MQ=255)

                cgcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCt                                                                         >  2:2954292/1‑93 (MQ=255)

                cgcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCt                                                                         <  1:1198821/93‑1 (MQ=255)

                   cAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCt                                                                      >  1:2901769/1‑93 (MQ=255)

                   cAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCt                                                                      <  1:2690788/93‑1 (MQ=255)

                     ggTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGt                                                                    <  2:6401617/93‑1 (MQ=255)

                     ggTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGt                                                                    >  2:4912723/1‑93 (MQ=255)

                      gTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTa                                                                   <  1:5203144/93‑1 (MQ=255)

                          gTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGGCTGGACGTCCGTGAAGCTTCTTTACTgg                                                               >  2:3423581/1‑93 (MQ=255)

                          gTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTgg                                                               <  2:2528564/93‑1 (MQ=255)

                             ttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTa                                                            <  1:269847/93‑1 (MQ=255)

                             ttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTa                                                            <  2:4497667/93‑1 (MQ=255)

                             ttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTa                                                            >  1:2084930/1‑93 (MQ=255)

                             ttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGGTa                                                            >  1:1257245/1‑93 (MQ=255)

                              tACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAc                                                           <  1:3151892/93‑1 (MQ=255)

                              tACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAc                                                           <  2:5626803/93‑1 (MQ=255)

                              tACCGATGATCAGCGCCGGCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAc                                                           <  1:5564526/93‑1 (MQ=255)

                               aCCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcg                                                          >  1:2513682/1‑93 (MQ=255)

                                ccGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcgc                                                         <  2:417781/93‑1 (MQ=255)

                                ccGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcgc                                                         >  1:4938717/1‑93 (MQ=255)

                                 cGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcgcg                                                        <  2:728130/93‑1 (MQ=255)

                                  gatgatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcgcgc                                                       <  1:6368833/93‑1 (MQ=255)

                                     gatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCt                                                    <  1:1134727/93‑1 (MQ=255)

                                     gatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCt                                                    <  2:5767280/93‑1 (MQ=255)

                                      gtCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCtt                                                   <  2:2118976/92‑1 (MQ=255)

                                      gtCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCtt                                                   <  2:3307039/92‑1 (MQ=255)

                                       tCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                  <  2:1312542/93‑1 (MQ=255)

                                       tCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTc                                                  >  2:2461722/1‑93 (MQ=255)

                                       tCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCCTc                                                  >  2:207696/1‑93 (MQ=255)

                                         aGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCg                                                <  2:3948972/93‑1 (MQ=255)

                                         aGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCg                                                >  1:621317/1‑93 (MQ=255)

                                         aGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCg                                                >  1:1864407/1‑93 (MQ=255)

                                          gcgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGa                                               >  2:3900498/1‑93 (MQ=255)

                                          gcgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGa                                               >  2:3041299/1‑93 (MQ=255)

                                           cgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                              <  1:549445/93‑1 (MQ=255)

                                           cgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                              <  1:463048/93‑1 (MQ=255)

                                           cgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGAt                                              <  2:1196462/93‑1 (MQ=255)

                                              cGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcac                                           <  2:4576909/93‑1 (MQ=255)

                                               gTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATcaca                                          >  1:123442/1‑93 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATc                                       >  2:4742671/1‑93 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATc                                       <  1:2420234/93‑1 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATc                                       >  1:353755/1‑93 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATc                                       <  2:2509527/93‑1 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTAGTTACGCGCGCTCCCGATCACAATc                                       >  1:5219219/1‑93 (MQ=255)

                                                   ggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATcc                                      >  1:1031267/1‑93 (MQ=255)

                                                      gTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCt                                   <  1:3077579/93‑1 (MQ=255)

                                                      gTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCt                                   >  2:4389968/1‑93 (MQ=255)

                                                      gTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCt                                   >  1:4143994/1‑93 (MQ=255)

                                                       tttGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTa                                  <  2:2334754/93‑1 (MQ=255)

                                                         tGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACt                                <  1:3680605/93‑1 (MQ=255)

                                                         tGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACt                                >  1:1411603/1‑93 (MQ=255)

                                                          ggTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTc                               <  2:1117921/93‑1 (MQ=255)

                                                          ggTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTc                               >  1:3950173/1‑93 (MQ=255)

                                                          ggTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTc                               <  2:6158410/93‑1 (MQ=255)

                                                          ggTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTc                               >  2:6325929/1‑93 (MQ=255)

                                                           gTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCg                              >  1:6050664/1‑93 (MQ=255)

                                                            tCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGt                             <  1:3324073/93‑1 (MQ=255)

                                                              gCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTg                           <  2:363259/93‑1 (MQ=255)

                                                               cccGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGa                          >  2:821580/1‑93 (MQ=255)

                                                               cccGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGa                          <  1:4443710/93‑1 (MQ=255)

                                                                ccGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGaa                         <  1:5882858/93‑1 (MQ=255)

                                                                ccGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGaa                         >  1:5754686/1‑93 (MQ=255)

                                                                 cGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAt                        <  1:1579217/93‑1 (MQ=255)

                                                                 cGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAt                        >  2:1593907/1‑93 (MQ=255)

                                                                  gCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                       <  2:16733/93‑1 (MQ=255)

                                                                   cTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTc                      <  2:3463027/93‑1 (MQ=255)

                                                                   cTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTc                      >  1:5117439/1‑93 (MQ=255)

                                                                     cGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAg                    >  1:2845774/1‑93 (MQ=255)

                                                                     cGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAg                    >  1:5042472/1‑93 (MQ=255)

                                                                       aTTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTc                  >  1:2140184/1‑93 (MQ=255)

                                                                        ttttGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTcc                 <  1:343754/93‑1 (MQ=255)

                                                                        ttttGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTcc                 <  2:1540788/93‑1 (MQ=255)

                                                                         tttGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTccc                <  2:4484052/93‑1 (MQ=255)

                                                                           tGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCgg              <  2:5673815/93‑1 (MQ=255)

                                                                            gTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc             <  1:5247311/93‑1 (MQ=255)

                                                                            gTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc             <  1:6002158/93‑1 (MQ=255)

                                                                              tttCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCaa           <  1:5382653/93‑1 (MQ=255)

                                                                                tCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc         <  2:3195903/93‑1 (MQ=255)

                                                                                tCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc         <  1:3803132/93‑1 (MQ=255)

                                                                                tCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc         >  1:750185/1‑93 (MQ=255)

                                                                                tCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc         >  1:1163965/1‑93 (MQ=255)

                                                                                 cTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGcc        <  2:3751945/93‑1 (MQ=255)

                                                                                 cTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGcc        <  1:4849778/93‑1 (MQ=255)

                                                                                  tACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt       <  2:4978893/93‑1 (MQ=255)

                                                                                   aCCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg      >  2:276119/1‑93 (MQ=255)

                                                                                    ccGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGa     <  1:4727046/93‑1 (MQ=255)

                                                                                     cGTGGGCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCAGCTACTCGTTGAATTCAGTCCCGGCAAGTCTGat    >  2:3335295/1‑93 (MQ=255)

                                                                                      gTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata   >  1:3226922/1‑93 (MQ=255)

                                                                                       tGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataa  >  2:4081193/1‑93 (MQ=255)

                                                                                       tGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataa  >  2:5859499/1‑93 (MQ=255)

GCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAA  >  NC_000913/231739‑231918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`