BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,486,394	T→C	A278A (GCA→GCG)	yfdE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,486,394	0	T	C	99.6%	838.0 / NA	238	A278A (GCA→GCG)	yfdE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (102/135); minor base A (0/1); total (102/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

CTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCAT  >  NC_000913/2486304‑2486483

cTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAgcgc                                                                                         >  1:5545404/1‑93 (MQ=255)

 tCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGcc                                                                                        >  1:1152535/1‑93 (MQ=255)

 tCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGcc                                                                                        <  1:3568701/93‑1 (MQ=255)

 tCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGcc                                                                                        <  2:2102921/93‑1 (MQ=255)

 tCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGcc                                                                                        <  1:1680729/93‑1 (MQ=255)

 tCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGcc                                                                                        <  1:854776/93‑1 (MQ=255)

     tatatTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGc                                                                                    >  2:6256872/1‑93 (MQ=255)

      atatTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCa                                                                                   <  1:1983986/93‑1 (MQ=255)

         ttGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATaa                                                                                <  2:6159195/93‑1 (MQ=255)

          tGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAAc                                                                               >  2:5901016/1‑93 (MQ=255)

            tttAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGc                                                                             <  2:4681/93‑1 (MQ=255)

              tAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAg                                                                           <  1:1469907/93‑1 (MQ=255)

               aaGAATAGCGTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGa                                                                          <  2:2385175/93‑1 (MQ=255)

                aGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGaa                                                                         >  2:2649854/1‑93 (MQ=255)

                aGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGaa                                                                         >  1:2376228/1‑93 (MQ=255)

                  aaTAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGaaaa                                                                       >  2:5073622/1‑93 (MQ=255)

                    tAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGaaaaaa                                                                     <  1:4778618/93‑1 (MQ=255)

                    tAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGaaaaaa                                                                     <  2:437041/93‑1 (MQ=255)

                     aGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAg                                                                    >  2:5749498/1‑93 (MQ=255)

                        cTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCtt                                                                 <  1:4633616/93‑1 (MQ=255)

                        cTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCtt                                                                 >  2:2774407/1‑93 (MQ=255)

                        cTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCtt                                                                 <  2:1750199/93‑1 (MQ=255)

                         tGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTg                                                                >  2:753587/1‑93 (MQ=255)

                         tGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTg                                                                >  1:1517810/1‑93 (MQ=255)

                         tGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTg                                                                <  1:989402/93‑1 (MQ=255)

                           gTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGGTCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTc                                                              <  2:3503369/93‑1 (MQ=255)

                           gTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTc                                                              <  1:2550393/93‑1 (MQ=255)

                           gTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGAGCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTc                                                              >  1:5939886/1‑93 (MQ=255)

                            ttttGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCa                                                             >  2:4674272/1‑93 (MQ=255)

                            ttttGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCa                                                             <  2:5814989/93‑1 (MQ=255)

                            ttttGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACAAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCa                                                             >  2:5278000/1‑93 (MQ=255)

                             tttGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCAt                                                            <  2:6099824/93‑1 (MQ=255)

                             tttGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCAt                                                            <  1:2935683/93‑1 (MQ=255)

                              ttGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCAtt                                                           <  1:2151880/93‑1 (MQ=255)

                              ttGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCAtt                                                           <  2:4200134/93‑1 (MQ=255)

                               tGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTa                                                          <  1:5394407/93‑1 (MQ=255)

                                   cgcgTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACcac                                                      <  1:1072824/93‑1 (MQ=255)

                                       tAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCacaaca                                                  >  1:1776241/1‑93 (MQ=255)

                                         aaaTATTGCTGCTAAATCGGGGATCATTAGCCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACaaa                                                <  1:6351026/93‑1 (MQ=255)

                                         aaaTATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACaaa                                                >  1:376921/1‑93 (MQ=255)

                                         aaaTATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACaaa                                                >  2:5508448/1‑93 (MQ=255)

                                          aaTATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAAt                                               <  2:4989704/93‑1 (MQ=255)

                                          aaTATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAAt                                               >  2:6210559/1‑93 (MQ=255)

                                          aaTATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAAt                                               <  2:6446599/93‑1 (MQ=255)

                                           atatTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATc                                              <  1:3692302/93‑1 (MQ=255)

                                            tatTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCg                                             >  2:3570151/1‑93 (MQ=255)

                                            tatTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCg                                             >  1:4427204/1‑93 (MQ=255)

                                                gctgctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAAt                                         <  1:5593878/93‑1 (MQ=255)

                                                gctgctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAAt                                         >  1:2668038/1‑93 (MQ=255)

                                                gctgctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAAt                                         <  1:2131180/93‑1 (MQ=255)

                                                 ctgctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATc                                        <  2:1124608/93‑1 (MQ=255)

                                                  tgctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCg                                       >  2:1930238/1‑93 (MQ=255)

                                                  tgctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCg                                       >  2:5549662/1‑93 (MQ=255)

                                                  tgctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCg                                       <  2:2857395/93‑1 (MQ=255)

                                                   gctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCgg                                      <  2:4979494/93‑1 (MQ=255)

                                                    ctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGc                                     >  2:2269999/1‑93 (MQ=255)

                                                    ctAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGc                                     <  2:3054757/93‑1 (MQ=255)

                                                     tAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCt                                    <  1:740329/93‑1 (MQ=255)

                                                     tAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCt                                    <  1:2430231/93‑1 (MQ=255)

                                                      aaaTCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCtt                                   >  1:5525525/1‑93 (MQ=255)

                                                       aaTCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTa                                  >  2:2478187/1‑93 (MQ=255)

                                                         tCGGGGATCATTAGCCAGTTCCGTAAGCTCCAGAGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATc                                <  2:3882474/93‑1 (MQ=255)

                                                         tCGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATc                                <  1:5149348/93‑1 (MQ=255)

                                                          cGGGGATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATcc                               <  1:3022541/93‑1 (MQ=255)

                                                           ggggATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCt                              <  2:856884/93‑1 (MQ=255)

                                                           ggggATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCt                              >  1:4746658/1‑93 (MQ=255)

                                                            gggATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTg                             >  1:1961545/1‑93 (MQ=255)

                                                             ggATCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGa                            <  1:4030801/93‑1 (MQ=255)

                                                               aTCATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGt                          >  2:1241057/1‑93 (MQ=255)

                                                                 cATTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTAt                        <  2:5938618/93‑1 (MQ=255)

                                                                  aTTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTAtt                       >  1:2417015/1‑93 (MQ=255)

                                                                  aTTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTAtt                       <  1:3439153/93‑1 (MQ=255)

                                                                  aTTAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTAtt                       <  1:1746409/93‑1 (MQ=255)

                                                                   ttAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTg                      >  2:3398340/1‑93 (MQ=255)

                                                                   ttAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTg                      <  2:1422673/93‑1 (MQ=255)

                                                                   ttAACCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTg                      <  1:416760/93‑1 (MQ=255)

                                                                     aaCCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGaa                    <  1:4668522/93‑1 (MQ=255)

                                                                     aaCCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGaa                    >  2:4566223/1‑93 (MQ=255)

                                                                      aCCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGaaa                   >  2:2566067/1‑93 (MQ=255)

                                                                      aCCAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCATGAGTATTGaaa                   >  2:4109311/1‑93 (MQ=255)

                                                                       ccAGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGaaaa                  <  2:1344668/93‑1 (MQ=255)

                                                                         aGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACa                <  1:6407555/93‑1 (MQ=255)

                                                                         aGTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACa                <  1:5239851/93‑1 (MQ=255)

                                                                          gTTCCGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACAt               <  1:6239867/93‑1 (MQ=255)

                                                                             ccGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCaa            <  1:2338457/93‑1 (MQ=255)

                                                                              cGTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCaaa           >  1:4650520/1‑93 (MQ=255)

                                                                               gTAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCaaaa          <  1:4358728/93‑1 (MQ=255)

                                                                                tAAGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAg         <  1:3908568/93‑1 (MQ=255)

                                                                                 aaGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAgg        <  2:1656414/93‑1 (MQ=255)

                                                                                  aGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGt       <  1:3376242/93‑1 (MQ=255)

                                                                                  aGCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGt       <  1:3204543/93‑1 (MQ=255)

                                                                                   gCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGtc      >  2:4784819/1‑92 (MQ=255)

                                                                                   gCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTg      >  2:3786879/1‑93 (MQ=255)

                                                                                   gCTCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTg      >  2:4876742/1‑93 (MQ=255)

                                                                                     tCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGcc    <  2:3149616/93‑1 (MQ=255)

                                                                                     tCCAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGcc    >  1:1353880/1‑93 (MQ=255)

                                                                                      ccAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACGACAAATCGTAATCGGCTTATCCTGAGGNNTGAAAGCATGAAAGNGttngt   >  2:3232287/1‑89 (MQ=255)

                                                                                      ccAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCa   >  1:4229784/1‑93 (MQ=255)

                                                                                      ccAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCa   >  2:1528246/1‑93 (MQ=255)

                                                                                       cAGCGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCAt  <  2:4506683/93‑1 (MQ=255)

CTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCAT  >  NC_000913/2486304‑2486483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`