BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,501,010	A→T	I499N (ATC→AAC)	b2383 ←	putative PTS system enzyme IIA component, enzyme I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,501,010	0	A	T	100.0%	842.1 / NA	228	I499N (ATC→AAC)	b2383	putative PTS system enzyme IIA component, enzyme I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (129/99); total (129/99)

Only 100 of 238 total aligned reads displayed.

TGACGCAGGCCATCGCGCTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGATCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTAAATACGT  >  NC_000913/2500920‑2501099

  aCGCAGGCCATCGCGCTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGttcat                                                                                       >  2:332624/1‑93 (MQ=255)

   cGCAGCCCATCGCGCTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGttcatc                                                                                      <  2:2740676/93‑1 (MQ=255)

      aGGCCATCGCGCTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAAc                                                                                   >  2:3562478/1‑93 (MQ=255)

          cATCGCGCTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTggg                                                                               >  2:218515/1‑93 (MQ=255)

           aTCGCGCTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGCGTTCATCAACTGGGc                                                                              >  1:6181297/1‑93 (MQ=255)

            tCGCGCTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCg                                                                             >  2:4423254/1‑93 (MQ=255)

              gcgcTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGtt                                                                           <  2:5921668/93‑1 (MQ=255)

              gcgcTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGtt                                                                           <  1:5641377/93‑1 (MQ=255)

               cgcTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTg                                                                          >  1:6334322/1‑93 (MQ=255)

                gcTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGc                                                                         <  1:2569423/93‑1 (MQ=255)

                 cTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGct                                                                        >  2:2149377/1‑92 (MQ=255)

                 cTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGcc                                                                        >  1:1368021/1‑93 (MQ=255)

                 cTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGcc                                                                        >  2:119795/1‑93 (MQ=255)

                 cTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGcc                                                                        >  2:1993908/1‑93 (MQ=255)

                  ttAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCg                                                                       <  1:2287385/93‑1 (MQ=255)

                   tAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGa                                                                      <  1:5254173/93‑1 (MQ=255)

                    aaGCTCAACGATCGCTTTTTGGATCTCGCATTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCgta                                                                     >  2:2318848/1‑91 (MQ=255)

                     aGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGaaa                                                                    >  1:5756503/1‑93 (MQ=255)

                      gCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAAc                                                                   <  2:3844174/93‑1 (MQ=255)

                      gCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAAc                                                                   <  2:4770675/93‑1 (MQ=255)

                        tCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTg                                                                 <  1:3117281/93‑1 (MQ=255)

                        tCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTg                                                                 >  1:3849859/1‑93 (MQ=255)

                         cAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTgg                                                                >  1:4507734/1‑93 (MQ=255)

                         cAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTgg                                                                <  1:1020073/93‑1 (MQ=255)

                          aaCGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTggc                                                               <  1:1719490/93‑1 (MQ=255)

                             gATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTggcggc                                                            <  2:5132051/93‑1 (MQ=255)

                              aTCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGgcggcg                                                           >  1:1150409/1‑93 (MQ=255)

                               tCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGgcgc                                                          <  2:4823232/93‑1 (MQ=255)

                               tCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGgcgc                                                          >  2:4653004/1‑93 (MQ=255)

                               tCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGgcgc                                                          >  1:3763345/1‑93 (MQ=255)

                                 gCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGgcgcgc                                                        >  1:4329717/1‑93 (MQ=255)

                                  cTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCa                                                       >  2:2305174/1‑93 (MQ=255)

                                   tttttGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCaa                                                      >  2:6411247/1‑93 (MQ=255)

                                   tttttGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCaa                                                      >  2:5487/1‑93 (MQ=255)

                                    ttttGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCaaa                                                     <  2:825633/93‑1 (MQ=255)

                                    ttttGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCaaa                                                     >  1:650419/1‑93 (MQ=255)

                                     tttGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGGGCGCaaaa                                                    >  2:323845/1‑93 (MQ=255)

                                     tttGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCaaaa                                                    <  2:6157518/93‑1 (MQ=255)

                                      ttGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAAt                                                   >  2:4294656/1‑93 (MQ=255)

                                         gATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGc                                                >  1:3543693/1‑93 (MQ=255)

                                         gATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGc                                                >  2:597795/1‑93 (MQ=255)

                                          aTCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGcc                                               <  2:5891625/93‑1 (MQ=255)

                                           tctcGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGccc                                              <  2:5788247/93‑1 (MQ=255)

                                           tctcGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAAATGGGGGCGCGCAAAATGGccc                                              >  1:5917354/1‑93 (MQ=255)

                                             tcGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGGGCGCAAAATGGGCCGc                                            >  1:4873174/1‑93 (MQ=255)

                                             tcGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGc                                            >  2:5335018/1‑93 (MQ=255)

                                             tcGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGc                                            <  2:5386549/93‑1 (MQ=255)

                                             tcGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGc                                            >  1:2141321/1‑93 (MQ=255)

                                             tcGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGc                                            >  1:757284/1‑93 (MQ=255)

                                              cGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCa                                           <  1:1944525/93‑1 (MQ=255)

                                               gCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAg                                          >  1:5713793/1‑93 (MQ=255)

                                               gCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAg                                          >  1:5953646/1‑93 (MQ=255)

                                               gCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAg                                          >  2:4257240/1‑93 (MQ=255)

                                                ccTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGt                                         <  1:5892776/93‑1 (MQ=255)

                                                ccTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGt                                         <  1:2091069/93‑1 (MQ=255)

                                                 cTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGtt                                        >  1:5441835/1‑93 (MQ=255)

                                                 cTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGtt                                        <  1:4234867/93‑1 (MQ=255)

                                                  tttCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTg                                       >  2:378441/1‑93 (MQ=255)

                                                  tttCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTg                                       >  2:4699010/1‑93 (MQ=255)

                                                  tttCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTg                                       >  1:1452218/1‑93 (MQ=255)

                                                     cACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGt                                    >  2:4505564/1‑93 (MQ=255)

                                                     cACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGt                                    <  1:3772835/93‑1 (MQ=255)

                                                      aCCCATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTg                                   <  1:3962720/93‑1 (MQ=255)

                                                         cATAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCgg                                <  1:20403/93‑1 (MQ=255)

                                                          aTAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGa                               <  2:4074028/93‑1 (MQ=255)

                                                          aTAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGa                               >  1:1236943/1‑93 (MQ=255)

                                                           tAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGaa                              >  2:4219102/1‑93 (MQ=255)

                                                           tAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGaa                              >  2:3966793/1‑93 (MQ=255)

                                                           tAAGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGaa                              <  1:4413401/93‑1 (MQ=255)

                                                             aGATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACa                            <  1:284112/93‑1 (MQ=255)

                                                              gATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAg                           >  1:5457905/1‑93 (MQ=255)

                                                              gATCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAg                           >  2:2566316/1‑93 (MQ=255)

                                                              gATCTAATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAg                           <  2:4157089/93‑1 (MQ=255)

                                                                tCTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGc                         >  2:2520991/1‑93 (MQ=255)

                                                                 cTGATCGAGTCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGcc                        <  1:3420772/93‑1 (MQ=255)

                                                                 cTGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGcc                        <  2:135524/93‑1 (MQ=255)

                                                                  tGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCa                       <  2:2927329/93‑1 (MQ=255)

                                                                  tGATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCa                       >  2:1552084/1‑93 (MQ=255)

                                                                   gATCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAg                      >  2:2841015/1‑93 (MQ=255)

                                                                    aTCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGc                     <  2:366892/93‑1 (MQ=255)

                                                                    aTCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGc                     <  2:3013319/93‑1 (MQ=255)

                                                                     tCGAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCa                    <  2:3686225/93‑1 (MQ=255)

                                                                       gAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCaaa                  <  1:4064801/93‑1 (MQ=255)

                                                                       gAGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCaaa                  >  2:6127388/1‑93 (MQ=255)

                                                                        aGGCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAAt                 >  2:3645529/1‑93 (MQ=255)

                                                                         ggCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAAtt                <  2:1764259/93‑1 (MQ=255)

                                                                         ggCTGTGAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAAtt                >  2:6071212/1‑93 (MQ=255)

                                                                             gtgAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCgg            <  1:3028964/93‑1 (MQ=255)

                                                                              tgAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACCGGCCAGCAACTTCCggg           >  2:162969/1‑93 (MQ=255)

                                                                              tgAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCggg           <  2:5751170/93‑1 (MQ=255)

                                                                               gAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGt          >  1:3405949/1‑93 (MQ=255)

                                                                               gAACCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGt          <  2:2454390/93‑1 (MQ=255)

                                                                                aaCCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTa         <  1:1541490/93‑1 (MQ=255)

                                                                                 aCCATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTaa        >  2:2896130/1‑93 (MQ=255)

                                                                                  ccATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTaaa       >  1:3372399/1‑93 (MQ=255)

                                                                                  ccATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTaaa       >  2:4323563/1‑93 (MQ=255)

                                                                                   cATCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTAAAt      <  1:4631890/93‑1 (MQ=255)

                                                                                    aTCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTAAATa     <  1:5113578/93‑1 (MQ=255)

                                                                                     tCGGGTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTAAATAc    >  2:4355645/1‑93 (MQ=255)

                                                                                       gggTTCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTAAATACGt  >  2:4866730/1‑93 (MQ=255)

TGACGCAGGCCATCGCGCTTAAGCTCAACGATCGCTTTTTGGATCTCGCCTTTCACCCATAAGATCTGATCGAGGCTGTGAACCATCGGGATCATCAACTGGGCGTTGCCGAAACTGGCGGCGCGCAAAATGGCCCGCAGTTGAGTGCGGAACAGGCCAGCAAATTCCGGGTAAATACGT  >  NC_000913/2500920‑2501099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`