BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	254,298	T→C	T473T (ACA→ACG)	pepD ←	aminoacyl‑histidine dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	254,298	0	T	C	100.0%	926.9 / NA	266	T473T (ACA→ACG)	pepD	aminoacyl‑histidine dipeptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (149/117); total (149/117)

Only 100 of 280 total aligned reads displayed.

GATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCAT  >  NC_000913/254208‑254387

   gAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCa                                                                                      >  1:499515/1‑93 (MQ=255)

   gAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCa                                                                                      <  1:1594239/93‑1 (MQ=255)

    aaaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAg                                                                                     <  1:4164261/93‑1 (MQ=255)

    aaaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAg                                                                                     <  2:6235326/93‑1 (MQ=255)

     aaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGt                                                                                    >  1:4010209/1‑93 (MQ=255)

     aaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGt                                                                                    >  2:5099/1‑93 (MQ=255)

      aCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTa                                                                                   >  1:2554633/1‑93 (MQ=255)

       cTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTaa                                                                                  >  2:4324246/1‑93 (MQ=255)

        tGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTGAt                                                                                 >  2:1400358/1‑93 (MQ=255)

        tGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAAt                                                                                 >  2:4246988/1‑93 (MQ=255)

        tGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAAt                                                                                 <  2:739262/93‑1 (MQ=255)

        tGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAAt                                                                                 >  2:650298/1‑93 (MQ=255)

        tGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAAt                                                                                 <  1:6092898/93‑1 (MQ=255)

            ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTAGTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGAcc                                                                             >  2:3432344/1‑93 (MQ=255)

            ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGAcc                                                                             <  2:5458767/93‑1 (MQ=255)

            ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGAcc                                                                             >  2:22559/1‑93 (MQ=255)

            ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGAcc                                                                             >  2:2949188/1‑93 (MQ=255)

            ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGAcc                                                                             <  2:3783882/93‑1 (MQ=255)

             gAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCt                                                                            >  2:4765784/1‑93 (MQ=255)

              aaGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTa                                                                           <  1:2618837/93‑1 (MQ=255)

              aaGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTa                                                                           <  1:5150823/93‑1 (MQ=255)

               aGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTCCTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTAc                                                                          <  2:3307959/93‑1 (MQ=255)

                ggCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACg                                                                         >  1:715324/1‑93 (MQ=255)

                 gCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGc                                                                        <  2:2869464/93‑1 (MQ=255)

                  cGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCt                                                                       <  2:789247/93‑1 (MQ=255)

                   gATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCtt                                                                      >  2:4548823/1‑93 (MQ=255)

                   gATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCtt                                                                      <  1:1018059/93‑1 (MQ=255)

                    aTTAACCGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCttt                                                                     <  1:2299069/93‑1 (MQ=255)

                    aTTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCttt                                                                     <  1:5712287/93‑1 (MQ=255)

                    aTTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCttt                                                                     <  1:3985924/93‑1 (MQ=255)

                     atAAACGCCATCCGTCAGCAAATCAAATAATTACTGCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTc                                                                    <  1:1445430/92‑1 (MQ=255)

                       aaaCGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGa                                                                  >  1:2740386/1‑93 (MQ=255)

                          cGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGAtgt                                                               <  1:696871/93‑1 (MQ=255)

                            ccATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGa                                                             >  2:5745311/1‑93 (MQ=255)

                            ccATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGa                                                             >  1:5458386/1‑93 (MQ=255)

                             cATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGaa                                                            >  1:3505840/1‑93 (MQ=255)

                              aTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAAc                                                           >  1:3714221/1‑93 (MQ=255)

                              aTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAAc                                                           >  2:6349750/1‑93 (MQ=255)

                              aTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAAc                                                           >  2:6391226/1‑93 (MQ=255)

                              aTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAAc                                                           >  2:4665565/1‑93 (MQ=255)

                                ccGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACtt                                                         <  2:177240/93‑1 (MQ=255)

                                ccGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACtt                                                         >  2:1497067/1‑93 (MQ=255)

                                ccGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACtt                                                         <  1:5919339/93‑1 (MQ=255)

                                 cGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTg                                                        >  2:4891597/1‑93 (MQ=255)

                                  ggCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGc                                                       <  1:491648/93‑1 (MQ=255)

                                  ggCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGc                                                       >  1:2894813/1‑93 (MQ=255)

                                   gcagcaAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCt                                                      <  2:3914820/93‑1 (MQ=255)

                                    cagcaAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTc                                                     >  2:3764401/1‑93 (MQ=255)

                                     agcaAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCa                                                    <  2:3221130/93‑1 (MQ=255)

                                     agcaAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCa                                                    >  1:441077/1‑93 (MQ=255)

                                       caaatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATc                                                  >  1:6051550/1‑93 (MQ=255)

                                       caaatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATc                                                  <  1:1934869/93‑1 (MQ=255)

                                       caaatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATc                                                  >  2:4840269/1‑93 (MQ=255)

                                       caaatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTCTCGATGTGAACTTGCTCATc                                                  >  2:505640/1‑93 (MQ=255)

                                        aaatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATcc                                                 <  1:4666498/93‑1 (MQ=255)

                                         aatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCg                                                <  1:6009021/93‑1 (MQ=255)

                                         aatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCg                                                >  2:2780177/1‑93 (MQ=255)

                                          atcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCCGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGATCTTGCTCCTCCgg                                               >  1:879605/1‑93 (MQ=255)

                                            caaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGgag                                             <  1:4778810/93‑1 (MQ=255)

                                             aaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGgaga                                            >  1:2800232/1‑93 (MQ=255)

                                             aaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGgaga                                            <  2:2062410/93‑1 (MQ=255)

                                             aaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGgaga                                            >  2:4824936/1‑93 (MQ=255)

                                             aaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGgaga                                            >  1:3627188/1‑93 (MQ=255)

                                              aataatTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGagag                                           >  1:1711873/1‑93 (MQ=255)

                                              aataatTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGagag                                           >  1:1318200/1‑93 (MQ=255)

                                               ataatTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATGCGGAGGgt                                          >  2:1164661/1‑93 (MQ=255)

                                                  atTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGtgtg                                       <  1:2254310/93‑1 (MQ=255)

                                                    tACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGa                                     <  1:6414125/93‑1 (MQ=255)

                                                      cTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGAcc                                   >  1:4304650/1‑93 (MQ=255)

                                                       ttCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCg                                  >  2:562860/1‑93 (MQ=255)

                                                       ttCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCg                                  >  2:4928289/1‑93 (MQ=255)

                                                        tCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCgg                                 >  1:3902771/1‑93 (MQ=255)

                                                          gCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTg                               >  2:567426/1‑93 (MQ=255)

                                                          gCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTg                               >  1:2329525/1‑93 (MQ=255)

                                                          gCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTg                               <  2:1727422/93‑1 (MQ=255)

                                                            cGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGAt                             <  2:3350720/93‑1 (MQ=255)

                                                               aaTTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGt                          >  1:1542349/1‑93 (MQ=255)

                                                                   tctttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGc                      <  2:2496102/93‑1 (MQ=255)

                                                                   tctttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGc                      >  1:5333377/1‑93 (MQ=255)

                                                                     tttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGccc                    <  2:3392415/93‑1 (MQ=255)

                                                                     tttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGccc                    >  2:601008/1‑93 (MQ=255)

                                                                     tttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGccc                    <  1:3419808/93‑1 (MQ=255)

                                                                     tttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGccc                    <  1:6450352/93‑1 (MQ=255)

                                                                      ttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCg                   <  1:2820146/93‑1 (MQ=255)

                                                                       tcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGa                  <  2:5145940/93‑1 (MQ=255)

                                                                       tcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGa                  <  2:5582576/93‑1 (MQ=255)

                                                                        cagcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGAt                 >  2:4373529/1‑93 (MQ=255)

                                                                        cagcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGAt                 >  1:3064354/1‑93 (MQ=255)

                                                                        cagcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCACGAt                 >  1:1113136/1‑93 (MQ=255)

                                                                        cagcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGGGTGGACCGGTGATAGTTGGCCCGAt                 >  1:1207456/1‑93 (MQ=255)

                                                                          gcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAg               >  2:223629/1‑93 (MQ=255)

                                                                              ttCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAAc           <  1:2032034/93‑1 (MQ=255)

                                                                                cagtcagCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCa         <  2:4154554/93‑1 (MQ=255)

                                                                                 agtcagCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCAt        >  2:1620737/1‑93 (MQ=255)

                                                                                  gtcagCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATg       >  2:5269261/1‑93 (MQ=255)

                                                                                  gtcagCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATg       >  2:3647058/1‑93 (MQ=255)

                                                                                    cagcagCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTc     <  2:1250018/93‑1 (MQ=255)

                                                                                     agcagcGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTcc    <  2:2581791/93‑1 (MQ=255)

                                                                                      gcagCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATACGGAGAGAGTGGACCGGTGATAGTTGGCCCGATAGCAACCATGTCCa   >  1:5979697/1‑93 (MQ=255)

                                                                                       cagCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCAt  <  2:1318200/93‑1 (MQ=255)

GATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCAT  >  NC_000913/254208‑254387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`