BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,319,717	A→G	noncoding (83/87 nt)	leuU ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,319,717	0	A	G	99.2%	914.0 / ‑3.5	268	noncoding (83/87 nt)	leuU	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (141/125); total (142/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 273 total aligned reads displayed.

GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA  >  NC_000913/3319627‑3319806

ggACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGcc                                                                                         <  2:915364/93‑1 (MQ=255)

ggACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGcc                                                                                         <  2:5153914/93‑1 (MQ=255)

ggACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGcc                                                                                         <  1:3151637/93‑1 (MQ=255)

 gACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCg                                                                                        >  2:3961197/1‑93 (MQ=255)

     agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGa                                                                                    >  1:795951/1‑93 (MQ=255)

      gatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAc                                                                                   <  2:2472971/93‑1 (MQ=255)

         acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACggg                                                                                <  1:403336/93‑1 (MQ=255)

         acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACggg                                                                                <  2:780858/93‑1 (MQ=255)

           agatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAc                                                                              <  1:1447828/93‑1 (MQ=255)

           agatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAc                                                                              <  1:675250/93‑1 (MQ=255)

           agatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAc                                                                              >  1:1889323/1‑93 (MQ=255)

            gatacCTGACACCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             <  1:1988430/93‑1 (MQ=255)

            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             <  2:3578359/93‑1 (MQ=255)

            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             >  2:291627/1‑93 (MQ=255)

            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             <  2:5204969/93‑1 (MQ=255)

               acCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGa                                                                          <  2:6424130/93‑1 (MQ=255)

                ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGaa                                                                         >  2:3005750/1‑93 (MQ=255)

                ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGaa                                                                         <  1:2592880/93‑1 (MQ=255)

                 cTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAc                                                                        >  1:1243777/1‑93 (MQ=255)

                 cTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAc                                                                        <  1:5394260/93‑1 (MQ=255)

                  tGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAcc                                                                       <  2:2447699/93‑1 (MQ=255)

                   gACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAccc                                                                      >  1:520010/1‑93 (MQ=255)

                   gACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAccc                                                                      <  2:1094158/93‑1 (MQ=255)

                    aCAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCg                                                                     <  2:4930194/93‑1 (MQ=255)

                     cAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGt                                                                    >  1:1058103/1‑93 (MQ=255)

                      aaCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTa                                                                   <  2:138500/93‑1 (MQ=255)

                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  >  1:1560845/1‑93 (MQ=255)

                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  >  1:3857003/1‑93 (MQ=255)

                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  >  2:2219621/1‑93 (MQ=255)

                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACGTGCACCCGTaa                                                                  >  1:2233525/1‑93 (MQ=255)

                        ccATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAg                                                                 >  1:2820108/1‑93 (MQ=255)

                         cATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGc                                                                >  1:4113224/1‑93 (MQ=255)

                          aTTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGcc                                                               >  1:1261202/1‑93 (MQ=255)

                          aTTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGcc                                                               >  1:3396643/1‑93 (MQ=255)

                           ttCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGccc                                                              <  1:2608779/93‑1 (MQ=255)

                            tCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCt                                                             <  1:1159527/93‑1 (MQ=255)

                            tCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCt                                                             >  1:5150499/1‑93 (MQ=255)

                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            >  2:4308452/1‑93 (MQ=255)

                                cAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                         >  2:2799180/1‑93 (MQ=255)

                                cAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                         <  2:4811355/93‑1 (MQ=255)

                                 aGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTgg                                                        <  2:2738903/93‑1 (MQ=255)

                                   aCCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                      <  2:1796509/93‑1 (MQ=255)

                                    ccAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCa                                                     <  2:1224755/93‑1 (MQ=255)

                                     cAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAACCGTAAGCCCTATTGGGCAc                                                    >  1:1186279/1‑93 (MQ=255)

                                      aGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACt                                                   >  2:4718817/1‑93 (MQ=255)

                                      aGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACt                                                   <  1:5021787/93‑1 (MQ=255)

                                       ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTa                                                  <  2:4562385/93‑1 (MQ=255)

                                          ccAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccc                                               >  2:3873193/1‑92 (MQ=255)

                                           cAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                              <  1:3553398/93‑1 (MQ=255)

                                            aaaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                             >  1:2658973/1‑93 (MQ=255)

                                              aaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTc                                           >  2:252174/1‑93 (MQ=255)

                                               aCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCa                                          <  1:3884041/93‑1 (MQ=255)

                                                cGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                         <  2:2995594/93‑1 (MQ=255)

                                                cGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                         >  2:6335674/1‑93 (MQ=255)

                                                cGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                         <  1:2103619/93‑1 (MQ=255)

                                                cGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACCTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                         >  1:2902514/1‑93 (MQ=255)

                                                   aaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGt                                      <  2:6275698/93‑1 (MQ=255)

                                                   aaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGt                                      <  2:4459642/93‑1 (MQ=255)

                                                    aaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTa                                     <  2:6025759/93‑1 (MQ=255)

                                                     aaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAg                                    >  1:1358084/1‑93 (MQ=255)

                                                      aaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGc                                   <  1:3296200/93‑1 (MQ=255)

                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                  >  1:6124701/1‑93 (MQ=255)

                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                  >  2:2583996/1‑93 (MQ=255)

                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATCGGGCACTACCACCTCAAGGTAGCg                                  <  1:6224245/93‑1 (MQ=255)

                                                        gACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgt                                 <  1:2500538/93‑1 (MQ=255)

                                                         aCGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtg                                >  2:4534543/1‑93 (MQ=255)

                                                          cGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtgt                               >  2:2000515/1‑93 (MQ=255)

                                                          cGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtgt                               <  1:5621868/93‑1 (MQ=255)

                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              <  1:2408067/93‑1 (MQ=255)

                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              >  2:3240653/1‑93 (MQ=255)

                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              <  1:15448/93‑1 (MQ=255)

                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              >  1:4833658/1‑93 (MQ=255)

                                                                tCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCa                         <  1:1931691/93‑1 (MQ=255)

                                                                tCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCa                         <  2:4913520/93‑1 (MQ=255)

                                                                 cAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCaa                        >  2:858554/1‑93 (MQ=255)

                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      >  2:1703636/1‑93 (MQ=255)

                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      >  2:5620264/1‑93 (MQ=255)

                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      <  2:4166359/93‑1 (MQ=255)

                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      >  1:461799/1‑93 (MQ=255)

                                                                     gTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcc                    >  1:1696536/1‑93 (MQ=255)

                                                                     gTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcc                    <  2:379028/93‑1 (MQ=255)

                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  1:3178037/1‑93 (MQ=255)

                                                                        tctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccacc                 >  1:5513442/1‑93 (MQ=255)

                                                                        tctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccacc                 >  2:3342078/1‑93 (MQ=255)

                                                                        tctTTTCTGGAATTTGGTGACGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccaca                 >  2:709417/1‑92 (MQ=255)

                                                                          ttttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCcaccac               >  2:1625874/1‑93 (MQ=255)

                                                                            ttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt             <  2:5460090/93‑1 (MQ=255)

                                                                            ttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt             <  1:4198423/93‑1 (MQ=255)

                                                                             tCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTc            <  1:5753644/93‑1 (MQ=255)

                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           >  1:2317811/1‑93 (MQ=255)

                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           <  2:6117878/93‑1 (MQ=255)

                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           <  1:743651/93‑1 (MQ=255)

                                                                               tGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCgg          >  2:1885580/1‑93 (MQ=255)

                                                                                ggAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc         <  1:965632/93‑1 (MQ=255)

                                                                                  aaTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       >  2:3039026/1‑93 (MQ=255)

                                                                                   aTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACg      >  1:6424129/1‑93 (MQ=255)

                                                                                    tttGGTGCCGAGGACGGGACTTGAACCCGTAAGACCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACAACCTCCGCACgg     >  1:3745036/1‑93 (MQ=255)

                                                                                      tGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACTGAt   <  1:3979224/93‑1 (MQ=255)

                                                                                      tGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAt   <  2:1133833/93‑1 (MQ=255)

                                                                                      tGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAt   >  1:5912519/1‑93 (MQ=255)

GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA  >  NC_000913/3319627‑3319806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`