BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	457,312	T→C	A221A (GCT→GCC)	clpX →	ATP‑dependent specificity component of clpP serine protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	457,312	0	T	C	100.0%	928.4 / NA	265	A221A (GCT→GCC)	clpX	ATP‑dependent specificity component of clpP serine protease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (138/127); total (138/127)

Only 100 of 288 total aligned reads displayed.

TAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTT  >  NC_000913/457222‑457401

 aaGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGtt                                                                                        >  2:160536/1‑93 (MQ=255)

 aaGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGtt                                                                                        >  2:2942475/1‑93 (MQ=255)

 aaGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGtt                                                                                        <  1:3206061/93‑1 (MQ=255)

  aGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTc                                                                                       >  1:1778046/1‑93 (MQ=255)

  aGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTc                                                                                       <  1:1915800/93‑1 (MQ=255)

   gTCAGACAACCCGTCCATTCCCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTcc                                                                                      <  1:733080/93‑1 (MQ=255)

   gTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGACGGCACGGTAGGTGCCGTTcc                                                                                      >  2:6437384/1‑93 (MQ=255)

   gTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTcc                                                                                      >  2:3011915/1‑93 (MQ=255)

     cAGACACCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCAc                                                                                    <  2:2336383/93‑1 (MQ=255)

     cAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCAc                                                                                    <  2:5616792/93‑1 (MQ=255)

      aGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCAcc                                                                                   >  1:5069424/1‑93 (MQ=255)

      aGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCAcc                                                                                   >  1:1114297/1‑93 (MQ=255)

      aGACAACCCGTCCATTACCCGAGACATTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCAcc                                                                                   <  2:6245838/93‑1 (MQ=255)

       gACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCg                                                                                  >  1:1853811/1‑93 (MQ=255)

       gACAACCCGTCCATTACAGGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCg                                                                                  >  1:1019206/1‑93 (MQ=255)

            cccGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAgg                                                                             >  2:6384637/1‑93 (MQ=255)

            cccGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAgg                                                                             >  2:2254933/1‑93 (MQ=255)

            cccGTCCATTACCCGACACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAgg                                                                             <  1:1695640/93‑1 (MQ=255)

             ccGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGt                                                                            <  2:4459815/93‑1 (MQ=255)

              cGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTg                                                                           >  1:5489340/1‑93 (MQ=255)

              cGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTg                                                                           <  1:2099105/93‑1 (MQ=255)

               gTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTgg                                                                          <  2:5819838/93‑1 (MQ=255)

               gTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTgg                                                                          >  1:6118854/1‑93 (MQ=255)

                 ccATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGc                                                                        <  2:1507330/93‑1 (MQ=255)

                  cATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCg                                                                       >  1:3787442/1‑93 (MQ=255)

                  cATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCCCATGGTGGGCg                                                                       >  1:4303577/1‑93 (MQ=255)

                   aTTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGt                                                                      >  2:3360455/1‑93 (MQ=255)

                   aTTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGt                                                                      <  1:3115327/93‑1 (MQ=255)

                     tACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTaa                                                                    >  2:5020949/1‑93 (MQ=255)

                     tACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTaa                                                                    <  2:4332159/93‑1 (MQ=255)

                        ccGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACa                                                                 >  2:3030926/1‑93 (MQ=255)

                        ccGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACa                                                                 <  2:2921974/93‑1 (MQ=255)

                          gagaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATc                                                               >  2:1806116/1‑93 (MQ=255)

                          gagaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATc                                                               >  2:940959/1‑93 (MQ=255)

                           agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCCTAAACATcc                                                              >  1:4935588/1‑93 (MQ=255)

                           acaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATcc                                                              <  2:2057629/91‑1 (MQ=255)

                            gaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCg                                                             >  1:3823893/1‑93 (MQ=255)

                             aCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCgc                                                            >  1:5757438/1‑93 (MQ=255)

                             aCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCgc                                                            <  2:600400/93‑1 (MQ=255)

                                tttCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCgcagc                                                         >  2:6121325/1‑93 (MQ=255)

                                 ttCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCgcagca                                                        >  2:76808/1‑93 (MQ=255)

                                  tCCGGTGACGGCGTACAGCAGGCACTTTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGcagcag                                                       <  2:2728137/93‑1 (MQ=255)

                                  tCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGcagcag                                                       >  2:830990/1‑93 (MQ=255)

                                     ggTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGaa                                                    <  1:5717877/93‑1 (MQ=255)

                                     ggTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGaa                                                    <  1:4033686/93‑1 (MQ=255)

                                     ggTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGaa                                                    <  1:39225/93‑1 (MQ=255)

                                       tGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGCAAACATCCGCAGCAGGAAtt                                                  >  2:376737/1‑93 (MQ=255)

                                        gAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTc                                                 >  1:140397/1‑93 (MQ=255)

                                          aGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCtt                                               >  1:5601376/1‑93 (MQ=255)

                                          aGGAGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCtt                                               <  1:5977322/93‑1 (MQ=255)

                                                aCAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGt                                         <  1:3482060/93‑1 (MQ=255)

                                                 catcagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGtt                                        <  2:3967733/90‑1 (MQ=255)

                                                 cagcagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGtt                                        <  2:6149825/93‑1 (MQ=255)

                                                 cagcagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGtt                                        <  2:4948089/93‑1 (MQ=255)

                                                  agcagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTg                                       >  2:1309788/1‑93 (MQ=255)

                                                  agcagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTg                                       >  2:1255414/1‑93 (MQ=255)

                                                  agcagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTg                                       >  1:5112852/1‑93 (MQ=255)

                                                    cagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGAt                                     <  1:1559098/93‑1 (MQ=255)

                                                     agGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATa                                    >  1:993427/1‑93 (MQ=255)

                                                     agGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATa                                    >  1:5108919/1‑93 (MQ=255)

                                                     agGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATa                                    >  2:3725659/1‑93 (MQ=255)

                                                     agGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGCGCACATCCGCAGCAGGAATTCTTGCAGGTTGATa                                    >  2:1906228/1‑93 (MQ=255)

                                                      ggCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATAc                                   <  2:313817/93‑1 (MQ=255)

                                                        cACTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACct                                 <  2:5362851/93‑1 (MQ=255)

                                                         aCTGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACctc                                >  2:16805/1‑93 (MQ=255)

                                                           tGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTa                              <  1:4644749/93‑1 (MQ=255)

                                                           tGTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTa                              <  2:410210/93‑1 (MQ=255)

                                                            gTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTaa                             <  2:4684272/93‑1 (MQ=255)

                                                            gTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTaa                             >  1:4798350/1‑93 (MQ=255)

                                                            gTTGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTaa                             <  1:4189654/93‑1 (MQ=255)

                                                             ttGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAg                            >  1:6242978/1‑93 (MQ=255)

                                                              tGAAACTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGa                           <  1:1309788/93‑1 (MQ=255)

                                                               gAAACTGATCGAAGGCACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGAt                          >  1:1430221/1‑93 (MQ=255)

                                                                aaaCTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATc                         >  1:5677718/1‑93 (MQ=255)

                                                                aaaCTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATc                         >  2:5130992/1‑93 (MQ=255)

                                                                aaaCTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGAAGGAATTCTTGCAGGTTGATACCTCTAAGATc                         >  1:2747841/1‑93 (MQ=255)

                                                                 aaCTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATcc                        >  2:909319/1‑93 (MQ=255)

                                                                 aaCTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATcc                        <  2:452637/93‑1 (MQ=255)

                                                                 aaCTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATcc                        >  2:2560379/1‑93 (MQ=255)

                                                                  aCTGATGGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATTCCTCTAAGATCCt                       <  2:5107963/93‑1 (MQ=255)

                                                                  aCTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCt                       <  1:441720/93‑1 (MQ=255)

                                                                   cTGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTg                      >  2:5484819/1‑93 (MQ=255)

                                                                    tGATCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGt                     <  1:2439914/93‑1 (MQ=255)

                                                                      aTCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGttt                   >  1:2617261/1‑93 (MQ=255)

                                                                       tCGAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTa                  >  2:5194512/1‑93 (MQ=255)

                                                                         gAAGGTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTAtt                >  1:64592/1‑93 (MQ=255)

                                                                            ggTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTtgt             <  2:2239339/93‑1 (MQ=255)

                                                                            ggTACGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTtgt             >  1:6390510/1‑93 (MQ=255)

                                                                               aCGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGc          <  2:4725898/93‑1 (MQ=255)

                                                                               aCGGTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGc          >  1:4504123/1‑93 (MQ=255)

                                                                                 ggTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCgg        <  1:2452661/93‑1 (MQ=255)

                                                                                  gTAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGt       <  1:3009360/93‑1 (MQ=255)

                                                                                   tAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTg      <  2:5696163/93‑1 (MQ=255)

                                                                                   tAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTg      <  2:3575189/93‑1 (MQ=255)

                                                                                   tAGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTg      <  2:2170711/93‑1 (MQ=255)

                                                                                    aGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGc     >  1:2210716/1‑93 (MQ=255)

                                                                                    aGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGc     >  1:6148020/1‑93 (MQ=255)

                                                                                    aGCTGCCGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAAATCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGc     >  1:6474454/1‑93 (MQ=255)

                                                                                       tgccgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCATGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGtt  <  1:1038614/93‑1 (MQ=255)

                                                                                       tgccgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGtt  <  2:187305/93‑1 (MQ=255)

TAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTT  >  NC_000913/457222‑457401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`