BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,114,335	T→C	A156A (GCA→GCG)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,114,335	0	T	C	100.0%	900.6 / NA	257	A156A (GCA→GCG)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (130/127); total (130/127)

Only 100 of 264 total aligned reads displayed.

TTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCT  >  NC_000913/4114246‑4114424

ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                        >  1:560951/1‑93 (MQ=255)

ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                        <  1:4485701/93‑1 (MQ=255)

 tGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCAc                                                                                       <  1:3826161/93‑1 (MQ=255)

    tgtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCt                                                                                    >  1:5005436/1‑93 (MQ=255)

     gtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTc                                                                                   >  1:5707231/1‑93 (MQ=255)

     gtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTc                                                                                   >  2:4669762/1‑93 (MQ=255)

      tAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTcg                                                                                  <  1:833274/93‑1 (MQ=255)

       aaTCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTcgc                                                                                 >  1:3866638/1‑93 (MQ=255)

         tCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCga                                                                               <  2:4069796/93‑1 (MQ=255)

            gTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGagag                                                                            >  2:1833768/1‑93 (MQ=255)

            gTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGagag                                                                            <  2:3058780/93‑1 (MQ=255)

               acaTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCt                                                                         >  2:4160849/1‑93 (MQ=255)

               acaTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCt                                                                         <  1:2087397/93‑1 (MQ=255)

               acaTGGACACGGCCCTGAGTCATTTACCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCt                                                                         <  2:6244689/93‑1 (MQ=255)

                  tGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTcc                                                                      >  2:5980462/1‑93 (MQ=255)

                   ggACACGGCGCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCca                                                                     <  1:3619190/93‑1 (MQ=255)

                   ggACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCCCGCTCGCGAGAGCCTTCca                                                                     >  1:1834167/1‑93 (MQ=255)

                   ggACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCca                                                                     >  1:6020764/1‑93 (MQ=255)

                     acacGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCcaca                                                                   >  2:2936907/1‑93 (MQ=255)

                     acacGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCcaca                                                                   >  2:2400111/1‑93 (MQ=255)

                       acGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATg                                                                 >  1:1704682/1‑93 (MQ=255)

                       acGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATg                                                                 <  2:1782764/93‑1 (MQ=255)

                       acGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATg                                                                 <  2:5242634/93‑1 (MQ=255)

                        cGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATgg                                                                <  1:337990/93‑1 (MQ=255)

                        cGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATgg                                                                >  1:3431615/1‑93 (MQ=255)

                         ggCCCTGAGTCATTTTCCAGATAAGCCACGTATCAAGCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGt                                                               >  2:3454896/1‑93 (MQ=255)

                         ggCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGt                                                               <  1:2376019/93‑1 (MQ=255)

                         cgCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGt                                                               <  2:1652525/92‑1 (MQ=255)

                             cTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAg                                                           >  2:5859881/1‑93 (MQ=255)

                              tGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCGATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAgg                                                          >  2:4173245/1‑93 (MQ=255)

                               gAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                         <  1:2852162/93‑1 (MQ=255)

                               gAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                         <  1:5804932/93‑1 (MQ=255)

                               gAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                         <  1:1373821/93‑1 (MQ=255)

                                aGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAt                                                        <  1:6162610/93‑1 (MQ=255)

                                aGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAt                                                        <  2:3589860/93‑1 (MQ=255)

                                 gTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATc                                                       >  1:3268799/1‑93 (MQ=255)

                                 gTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATc                                                       <  1:4842430/93‑1 (MQ=255)

                                    aTTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGTCGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcac                                                    <  2:3437195/93‑1 (MQ=255)

                                     ttttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAcccctt                                                   >  1:1294538/1‑90 (MQ=255)

                                     ttttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcact                                                   >  1:4183920/1‑93 (MQ=255)

                                      tttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcactt                                                  >  2:3208655/1‑93 (MQ=255)

                                      tttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcactt                                                  <  1:4647666/93‑1 (MQ=255)

                                          cAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcact                                              <  2:1031571/93‑1 (MQ=255)

                                           aGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                             >  1:2989932/1‑93 (MQ=255)

                                           aGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                             <  2:1341234/93‑1 (MQ=255)

                                           aGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                             <  2:3069587/93‑1 (MQ=255)

                                           aGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                             >  1:6262307/1‑93 (MQ=255)

                                            gATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACttt                                            >  2:219786/1‑93 (MQ=255)

                                            gATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACttt                                            >  1:1813230/1‑93 (MQ=255)

                                                 gCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc                                       >  2:4242036/1‑93 (MQ=255)

                                                 gCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc                                       >  1:787235/1‑93 (MQ=255)

                                                   cACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCa                                     >  2:5762320/1‑93 (MQ=255)

                                                    aCGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAg                                    >  2:6478665/1‑93 (MQ=255)

                                                      gTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaa                                  <  1:2981855/93‑1 (MQ=255)

                                                       tATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaaa                                 >  2:4985136/1‑93 (MQ=255)

                                                          cAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGt                              <  2:2718087/93‑1 (MQ=255)

                                                           aaCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTa                             <  2:2683075/93‑1 (MQ=255)

                                                           aaCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTa                             <  1:1911727/93‑1 (MQ=255)

                                                           aaCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTa                             >  1:3140120/1‑93 (MQ=255)

                                                            aCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTAc                            >  1:5337774/1‑93 (MQ=255)

                                                            aCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTAc                            <  2:3895561/93‑1 (MQ=255)

                                                             ccGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACg                           >  2:763816/1‑93 (MQ=255)

                                                             ccGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACg                           <  1:398102/93‑1 (MQ=255)

                                                              cGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACgg                          <  2:6417181/93‑1 (MQ=255)

                                                              cGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACgg                          <  1:2474668/93‑1 (MQ=255)

                                                              cGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACgg                          <  1:668552/93‑1 (MQ=255)

                                                               gTACCAAACAGCAATTCGCCACGACGCGCACGCTCGCCAGAGCCTTCCACATGGTCGAGAATCCACTTTACTTTGGTGCCAGAAAAGTACggg                         <  2:542494/93‑1 (MQ=255)

                                                               gTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACggg                         <  1:2203690/93‑1 (MQ=255)

                                                               gTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACggg                         <  1:4312873/93‑1 (MQ=255)

                                                                tACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGt                        >  1:3012714/1‑93 (MQ=255)

                                                                 aCCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTc                       <  2:5815923/93‑1 (MQ=255)

                                                                 aCCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTc                       >  1:165075/1‑93 (MQ=255)

                                                                 aCCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTc                       >  1:5473536/1‑93 (MQ=255)

                                                                  ccAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCa                      <  2:5478801/93‑1 (MQ=255)

                                                                  ccAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCa                      >  1:2613697/1‑93 (MQ=255)

                                                                    aaaCAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAAt                    >  2:1256274/1‑93 (MQ=255)

                                                                    aaaCAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAAt                    >  2:2121491/1‑93 (MQ=255)

                                                                     aaCAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATc                   <  2:3122351/93‑1 (MQ=255)

                                                                       cAGCAATTCACCACGACGCGCACGCTGGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCAc                 <  1:5202105/93‑1 (MQ=255)

                                                                            aTTTACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGAc            >  1:5083941/1‑93 (MQ=255)

                                                                            aTTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGAc            >  1:1054155/1‑93 (MQ=255)

                                                                            aTTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGAc            <  2:4022837/93‑1 (MQ=255)

                                                                            aTTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGAc            <  1:3303808/93‑1 (MQ=255)

                                                                              tCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCg          <  1:2298813/93‑1 (MQ=255)

                                                                              tCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCg          >  1:5973185/1‑93 (MQ=255)

                                                                               caccacGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCgg         <  2:6101769/93‑1 (MQ=255)

                                                                                accacGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGt        >  2:1410449/1‑93 (MQ=255)

                                                                                  cacGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAt      <  1:988595/93‑1 (MQ=255)

                                                                                  cacGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAt      <  2:2810267/93‑1 (MQ=255)

                                                                                   acgacgCTCACGCCCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt     <  1:1156841/93‑1 (MQ=255)

                                                                                   acgacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt     >  1:6110400/1‑93 (MQ=255)

                                                                                   acgacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt     >  2:1497008/1‑93 (MQ=255)

                                                                                    cgacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtg    >  1:4867210/1‑93 (MQ=255)

                                                                                    cgacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAACCACCAGACCGGTATtg    <  1:1728587/93‑1 (MQ=255)

                                                                                     gacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgc   >  2:4620381/1‑93 (MQ=255)

                                                                                     gacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgc   >  2:5806893/1‑93 (MQ=255)

                                                                                     gacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgc   <  2:1096064/93‑1 (MQ=255)

                                                                                     gacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgc   <  2:200584/93‑1 (MQ=255)

                                                                                      acgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgct  >  2:5183200/1‑93 (MQ=255)

                                                                                      acgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgct  <  1:2723505/93‑1 (MQ=255)

TTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCT  >  NC_000913/4114246‑4114424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`