BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,155,035	A→G	T203A (ACG→GCG)	argH →	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,155,035	0	A	G	99.6%	785.4 / ‑4.6	233	T203A (ACG→GCG)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (96/136); total (96/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

Only 100 of 242 total aligned reads displayed.

TTGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGT  >  NC_000913/4154946‑4155124

ttGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCgg                                                                                        >  1:1057584/1‑93 (MQ=255)

  gagaTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGcc                                                                                      <  1:5650435/93‑1 (MQ=255)

  gagaTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGcc                                                                                      <  1:4699185/93‑1 (MQ=255)

   agaTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCt                                                                                     <  1:3657898/93‑1 (MQ=255)

    gaTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTa                                                                                    <  2:6277598/93‑1 (MQ=255)

    gaTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTa                                                                                    >  2:3519602/1‑93 (MQ=255)

    gaTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTa                                                                                    <  1:1536428/93‑1 (MQ=255)

    gaTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTa                                                                                    <  1:5032988/93‑1 (MQ=255)

     aTGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTAt                                                                                   <  2:5916536/93‑1 (MQ=255)

      tGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATg                                                                                  >  1:2688079/1‑93 (MQ=255)

       gCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGa                                                                                 >  1:1687541/1‑93 (MQ=255)

         tGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGaaa                                                                               >  1:4787818/1‑93 (MQ=255)

         tGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGaaa                                                                               <  1:183993/93‑1 (MQ=255)

         tGGCGCGTCATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGaaa                                                                               <  1:5827632/93‑1 (MQ=255)

          ggCGCGTGATGAAAGCGGTTTGCAGGATTCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAAt                                                                              <  1:5051839/93‑1 (MQ=255)

          ggCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAAt                                                                              >  2:1603811/1‑93 (MQ=255)

          ggCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAAt                                                                              >  1:787514/1‑93 (MQ=255)

          ggCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAAt                                                                              <  1:2557117/93‑1 (MQ=255)

            cgcgTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCg                                                                            >  2:4509174/1‑93 (MQ=255)

              cgTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGAc                                                                          <  2:1200087/93‑1 (MQ=255)

               gTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGAcc                                                                         >  2:5870938/1‑93 (MQ=255)

               gTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGAcc                                                                         <  1:5377433/93‑1 (MQ=255)

                tgatgaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCg                                                                        >  2:1171291/1‑93 (MQ=255)

                tgatgaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCg                                                                        <  1:5857420/93‑1 (MQ=255)

                tgatgaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCg                                                                        >  1:3757888/1‑93 (MQ=255)

                    gaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCTACCGTGaa                                                                    >  2:2541354/1‑93 (MQ=255)

                    gaAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGaa                                                                    >  1:3973203/1‑93 (MQ=255)

                       aGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAg                                                                 <  1:3994851/93‑1 (MQ=255)

                       aGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAg                                                                 <  1:4956753/93‑1 (MQ=255)

                        gCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGt                                                                >  1:1870980/1‑93 (MQ=255)

                          cGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTa                                                              <  2:3956115/93‑1 (MQ=255)

                           gTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAg                                                             <  1:5572087/93‑1 (MQ=255)

                            tttGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGCACAGTTAGc                                                            >  2:4065900/1‑93 (MQ=255)

                             ttGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCa                                                           <  1:2655147/93‑1 (MQ=255)

                             ttGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCa                                                           <  1:5514436/93‑1 (MQ=255)

                              tGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAg                                                          >  2:5033389/1‑93 (MQ=255)

                               gCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAgg                                                         >  1:4488836/1‑93 (MQ=255)

                                cAGGATGCGCTTAAGCGTCTGGATGTCAGCCGGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggc                                                        <  1:2388968/93‑1 (MQ=255)

                                cAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggc                                                        <  1:6423101/93‑1 (MQ=255)

                                cAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggc                                                        <  1:4963440/93‑1 (MQ=255)

                                cAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggc                                                        <  1:2010718/93‑1 (MQ=255)

                                 aGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggct                                                       >  2:3320652/1‑93 (MQ=255)

                                   gATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggctgg                                                     >  2:2017500/1‑93 (MQ=255)

                                    aTGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggctggc                                                    >  2:2135616/1‑93 (MQ=255)

                                    aTGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAggctggc                                                    <  2:1369664/93‑1 (MQ=255)

                                       cgcTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGctggctgg                                                 <  1:435253/93‑1 (MQ=255)

                                       cgcTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGctggctgg                                                 <  1:1257675/93‑1 (MQ=255)

                                          ttAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCt                                              >  2:1873763/1‑93 (MQ=255)

                                          ttAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCt                                              <  1:999098/93‑1 (MQ=255)

                                           tAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCtt                                             >  1:3440564/1‑93 (MQ=255)

                                           tAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCtt                                             <  2:3890941/93‑1 (MQ=255)

                                            aaGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCttt                                            >  1:4824711/1‑93 (MQ=255)

                                             aGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTg                                           >  1:2185157/1‑93 (MQ=255)

                                             aGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTg                                           >  1:4327773/1‑93 (MQ=255)

                                              gCGTCTGGTTGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGGGCGGCCTATGAAATCCACCGTGAACAGATAGCAGGCTGGCTTGGCTTTGc                                          >  1:6318764/1‑93 (MQ=255)

                                              gCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGc                                          <  1:3259866/93‑1 (MQ=255)

                                               cGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCt                                         >  1:965110/1‑93 (MQ=255)

                                                 tCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTc                                       <  1:1437256/93‑1 (MQ=255)

                                                  cTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCg                                      <  1:5716105/93‑1 (MQ=255)

                                                  cTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCg                                      <  2:4128902/93‑1 (MQ=255)

                                                   tGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCgg                                     >  2:3802212/1‑93 (MQ=255)

                                                    ggATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGCACAGTTAGCAGGCCGGCTGGGCTTTGCTTCggg                                    >  2:4377181/1‑92 (MQ=255)

                                                    ggATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGc                                    <  2:4887867/93‑1 (MQ=255)

                                                    ggATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGc                                    <  1:4086217/93‑1 (MQ=255)

                                                    ggATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGc                                    <  2:248227/93‑1 (MQ=255)

                                                     gATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCcgcg                                   >  1:1044527/1‑93 (MQ=255)

                                                       tGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGAc                                 >  1:1114171/1‑93 (MQ=255)

                                                         tCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGGTTTGCTTCGGCGAccc                               >  1:3524601/1‑93 (MQ=255)

                                                            gCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTa                            >  1:3600937/1‑93 (MQ=255)

                                                            gCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTa                            <  2:4915735/93‑1 (MQ=255)

                                                            gCCCGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTa                            >  2:365388/1‑93 (MQ=255)

                                                               cGCTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACa                         <  2:531858/93‑1 (MQ=255)

                                                                 cTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGt                       >  2:5388401/1‑93 (MQ=255)

                                                                 cTAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGt                       >  2:5092536/1‑93 (MQ=255)

                                                                  tAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtc                      <  2:765170/93‑1 (MQ=255)

                                                                  tAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtc                      >  1:700409/1‑93 (MQ=255)

                                                                  tAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtc                      <  1:3942576/93‑1 (MQ=255)

                                                                  tAGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtc                      <  2:2185557/93‑1 (MQ=255)

                                                                   aGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtct                     >  2:2402040/1‑93 (MQ=255)

                                                                   aGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtct                     <  1:4509174/93‑1 (MQ=255)

                                                                   aGGCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtct                     <  2:2685350/93‑1 (MQ=255)

                                                                    ggCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtctc                    >  1:1281973/1‑93 (MQ=255)

                                                                    ggCTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGtctc                    >  1:3371589/1‑93 (MQ=255)

                                                                    ggCTGTGGCGCGCTGGCGGGAGCGGACTATGAAATCGAGCGTGAACAGTTAGCAGGCTGGGTGGGCTTTGCTTCGGCGACCCGTAACAGcctt                    >  2:4762407/1‑92 (MQ=255)

                                                                      cTGTGGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGa                  <  1:2950126/93‑1 (MQ=255)

                                                                       tgtgGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGAc                 <  1:929428/93‑1 (MQ=255)

                                                                        gtgGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACa                <  1:4238962/93‑1 (MQ=255)

                                                                         tgGCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAg               <  1:3132988/93‑1 (MQ=255)

                                                                          ggCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGGACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGc              >  2:2380527/1‑93 (MQ=255)

                                                                          ggCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGc              >  2:3239242/1‑93 (MQ=255)

                                                                          ggCGCGCTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGc              <  2:2803354/93‑1 (MQ=255)

                                                                           gcgcgcTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCg             >  2:222911/1‑93 (MQ=255)

                                                                            cgcgcTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGt            <  2:5570138/93‑1 (MQ=255)

                                                                             gcgcTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGtt           <  1:2050140/93‑1 (MQ=255)

                                                                               gcTGGCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTc         <  2:2842140/93‑1 (MQ=255)

                                                                                   gtgggAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGAc     <  1:3024927/91‑1 (MQ=255)

                                                                                   gCGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGAc     <  2:3170233/93‑1 (MQ=255)

                                                                                    cGGGAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGAcc    >  2:341155/1‑93 (MQ=255)

                                                                                     gggAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCg   >  2:1724629/1‑93 (MQ=255)

                                                                                      ggAGCGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCTTAACAGTCTCGACAGCGTTTCTGACCGt  <  1:657552/93‑1 (MQ=255)

TTGAGATGCTGGCGCGTGATGAAAGCCGTTTGCAGGATGCGCTTAAGCGTCTGGATGTCAGCCCGCTAGGCTGTGGCGCGCTGGCGGGAACGGCCTATGAAATCGACCGTGAACAGTTAGCAGGCTGGCTGGGCTTTGCTTCGGCGACCCGTAACAGTCTCGACAGCGTTTCTGACCGT  >  NC_000913/4154946‑4155124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`