Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2480GC32.1% 283.6 / 116.6 196intergenic (+148/+225)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base G (68/65);  new base C (63/0);  total (131/65)
Fisher's exact test for biased strand distribution p-value = 2.05e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 199 total aligned reads displayed.

TGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAC  >  NZ_CP025268/2233111‑2233386
                                                                                                                                           |                                                                                                                                           
tGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCTGGGGGCGGCCAAAAGCCGCCCCCCCCAGCCACCCCCgg                                                                                                                                            >  1:4843419/1‑140 (MQ=37)
   catcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGctc                                                                                                                                        <  1:4678934/140‑1 (MQ=255)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCACGGAGCGCGGCGGGGGGGGCCCAAACCCCCCCCCCCCCCC‑CCCCCCCggggcg                                                                                                                                        >  1:5300668/1‑137 (MQ=255)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCCGGGGGGGCCCGACGCCGCCGCCCCCCC‑CCCCCCCGGGctct                                                                                                                                       >  1:9476038/1‑140 (MQ=37)
         gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGCCCAAAGCCGCCGCCCCCAG‑CACCCCCCGGCTCAGGCGa                                                                                                                                  >  2:4434983/1‑140 (MQ=25)
         gTCCGAAAATAACGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGGGCCTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCAG‑CACCCCCGGGCTCGGGCgc                                                                                                                                  >  2:10121092/1‑139 (MQ=25)
          tCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGGTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTGTggagtc                                                                                                                                 >  2:10203581/1‑136 (MQ=255)
            cGAAAATAACGAGACTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAG‑CACCCCCGGGCTCTGGCGAACa                                                                                                                               >  2:11093095/1‑140 (MQ=255)
            cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCAGGCGAACa                                                                                                                               >  2:6704944/1‑140 (MQ=255)
            cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGGCGCCGCCCCCTG‑CACCCCCGGGCACATGCGAACa                                                                                                                               >  2:5943793/1‑140 (MQ=25)
              aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGCGGGCCAAAGCCGCCCCCCCCCC‑CACCCCCCCGCGCAGGCGAACaaa                                                                                                                             >  1:3843397/1‑140 (MQ=255)
              aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGGCCATCGCCGCCGCCCCCCG‑CCCCCCCCGGCGCAGGCGCACcaa                                                                                                                             >  2:3484455/1‑140 (MQ=255)
              aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGACGGGGGGGCCCATCGCCGCCGCCCCCAG‑CCCCCCCCGGCGCTGGccgccaaa                                                                                                                             >  1:634605/1‑133 (MQ=255)
                   aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                                                        <  2:541106/140‑1 (MQ=255)
                   aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATagc                                                                                                                        >  2:7803286/1‑140 (MQ=255)
                        gactatTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGCCAATCGCCCCCGCCCCCAG‑CCCCCCCCGGCACTGGCGAACAAAATCGCCGCTg                                                                                                                   >  2:6258960/4‑140 (MQ=21)
                            atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                                                               <  1:10684746/140‑1 (MQ=255)
                             tatCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAATGAGCGC‑GCAGGGGGCCGCCAAAACCCGCCCCCCCCAG‑CACCCCCCGGCTCTGGGGCACAAAATACCCGCTGCGCgg                                                                                                              >  2:8923550/3‑140 (MQ=255)
                                 aGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGcc                                                                                                          <  1:9250177/140‑1 (MQ=255)
                                 aGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGcc                                                                                                          <  1:9249726/140‑1 (MQ=255)
                                  gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAGCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                                         <  1:5494378/140‑1 (MQ=255)
                                  gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTACATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                                         <  1:2999030/140‑1 (MQ=255)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAG‑CACCCCCCGGCGCTGGCGAACAAAATAGCCCCTGCCCGGTGccca                                                                                                        >  2:10654269/1‑139 (MQ=18)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCCAAAGCCCCCCCCCCCAC‑CACCCCCCCGCGCATGCCAACAAAAAAACCCCAGCGCGGTGccca                                                                                                        >  2:4334754/1‑139 (MQ=16)
                                     tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGGCAAACGCCGCCGCCCCCTG‑CACCCCCGCGCGCTTGCGAACAAAATCGCCGCGGCGCGGTGCCCACg                                                                                                      >  2:7130509/1‑140 (MQ=18)
                                     tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATAGCCGCCGCCCCCAG‑CACCCCCCGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCg                                                                                                      >  2:6421409/1‑140 (MQ=255)
                                     tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAAGGGGCGGCCAAAAGCCGCCCCCCCCAA‑CACCCCCCGGCTAATGCGAAAAAAAACACCCCAGCACGGTTCCCACg                                                                                                      >  2:3699297/1‑140 (MQ=16)
                                       tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCCCCCCCCCCCG‑CACCCCCCCGCAAATGCCAAAAAAAAAACCCCAGCGCGGTGCCCACGGc                                                                                                    >  1:1814005/1‑140 (MQ=16)
                                       tcATCCTCTTGCGCCCCTGTCATGTGCATTGCGTCAGAGAAGCACTGGCGCCAGGAGCGC‑GCCCGGGGCGGCCAATCGCCGCCGGCCCCAG‑CCCCCCCCCGCTCGGGCGAACAAAAGCGCCGCTGCGCGGTGCCCGCggg                                                                                                    >  2:789209/1‑139 (MQ=17)
                                        cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGGCAAAACCCCCCGCCCCCAC‑CACCCCCCCGCACAGGCGAACAAAAAAGCCCCAGCCCGGTGCCCACGGcg                                                                                                   >  2:4158043/1‑139 (MQ=16)
                                        cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCATGGGGCGGCCCAACGCCCCCCCCCCCAG‑CACCCCCCCGCGCATGCGAACAAAAAAACCCCAGCGCCGTGcccaacgct                                                                                                   >  2:4158171/1‑134 (MQ=16)
                                          tCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                                                 <  1:1953430/140‑1 (MQ=255)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCATAGCCGCCCCCCCCCG‑CACCCCCCGGCTCAGGCGAACAAAAAAGCCCCGGCGCGGTGCCCACGGCGTaaacca                                                                                            >  2:2143302/1‑135 (MQ=18)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCt                                                                                           >  2:10641688/1‑140 (MQ=255)
                                                 gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGCCCAAACCCCCCCCCCCCCG‑CACCCCCCCGCGCAGGGGAAAAAAAAAACCCCAGAGCGGGGGCCCCGGCTTAT‑ACCCTa                                                                                         >  1:11412340/1‑140 (MQ=255)
                                                 gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGGCAAACGCCGCCGCCCCCAG‑CCCCCCCGGGCGCTGGCGAACAAAATCGCCGCGGCCCGGTGCCCCCGGCAGAT‑cccaga                                                                                         >  2:1845843/1‑137 (MQ=18)
                                                 gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATAGCCGCCGCCCCCAG‑CACCCCCCGGCACAGGCGCACAAAAACGCCGCAGCGCGGTGCCCACGGCTTaaaccata                                                                                          >  2:9283944/1‑133 (MQ=18)
                                                 gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTa                                                                                         <  1:9777986/140‑1 (MQ=255)
                                                   gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAG‑CACCCCCGGGCGCTGGCGAACAAAATCGCCGCGGCGCGGTGCCCTCGGCGTAT‑CCCTGACg                                                                                       >  2:1922458/1‑140 (MQ=18)
                                                     tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGGGGGCAAACCCCCCCCCCCCCAG‑AAACCCCCGGCGCAGGCGAAAAAAAAAGCCCCAGGCCGGGGCCCACGCCTTAT‑CCCATaatgc                                                                                     >  1:7917391/1‑136 (MQ=255)
                                                       cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGCCCAACGCCGCCCCCCCCCG‑CACCCCCCGGCGCAGGCGAACAAAAACACCGCGGCGCGGGGCCCACGGCGTAT‑ACCAAAAGGCTa                                                                                   >  2:1341783/1‑140 (MQ=12)
                                                         ttCATGTGCATTGCTTCATATAATCACGGGCGCATGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTAcc                                                                                 <  1:52489/139‑1 (MQ=255)
                                                         gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGGGGCCCAAACCCGCCCCCCCCTG‑GCCCCCCCGGGGCTTGCGAACCAAAACGCCGCTGCGCGGGGGCCACGGCGTAA‑CCCATAACGCTAcc                                                                                 >  2:2598267/1‑140 (MQ=12)
                                                         gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGCCAATAGCCGCCGGCCCCAG‑CACCCCCCGGCTCTGGCGGACAAAATCGCCGCGGCGCGGTGGCCACGGCGTAT‑ACCTTACGGCTTcc                                                                                 >  2:10814815/1‑140 (MQ=17)
                                                         gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCGCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTAcc                                                                                 >  2:11614813/1‑140 (MQ=255)
                                                         gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATAGCCGCTGCGCGGTGCCCTCGGCGTAA‑ACCATACGGCTaac                                                                                 >  2:5948804/1‑138 (MQ=17)
                                                            aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCCCCGCCCCCCG‑CACCCCCGGGCTCAGGCGAACAAAATCGCCCCTGCGCGGTGCCCACGGCGTAT‑CCCATACGGCGACCggg                                                                              >  2:971891/1‑140 (MQ=17)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAG‑CACCCCCGGGCTCAGGCGAACCAAATCGCCGCTGCGCGGTGCCCACGGCGTTT‑CCCTTACGGCGACCGGGGCg                                                                           >  2:11526937/1‑140 (MQ=14)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATATCCGCCGCCCCCAC‑CACCCCCCGGCTCTGGCGACCAAAATACCCGCTGCGCGGTGCCCACGGCGTAT‑CCCGTACTGCTACCGCGTCg                                                                           >  2:748512/1‑140 (MQ=14)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCCCCCCCAG‑CACCCCCCGGCTCATGCGAACAAAATCGCCGCTGCGCGGTGCCCACCGCTTAT‑ACCTTACGGCTACCCGGTCg                                                                           >  2:11305922/1‑140 (MQ=14)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCCCCCCCAG‑CACCCCCCGGCGCTGGCGCACCAAAAAGCCGCGGCGCGGTGCCCGCGGCTTAA‑ACCATAAGGCTACCcgggag                                                                           >  2:5846238/1‑137 (MQ=9)
                                                                 cagtGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCggg                                                                         <  2:8341173/137‑1 (MQ=255)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCCGGGACCGCCAAACCCCCCCCCCCCCGG‑CAACCCCCGGCTCAGGCCCACAACAACCCCGCAGCGCCGTGCCCACGCCAGCT‑ACCTTACCGCTAACcgggcggg                                                                         >  2:10817790/1‑140 (MQ=9)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCggg                                                                         <  2:8499494/140‑1 (MQ=255)
                                                                   ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGgcg                                                                       <  1:639762/140‑1 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGGCAAACGCCGCCCCCCCCAG‑CACCCCCCGGCACATGCCAACAAAATAGACGCGGCGCGGTGCCCTCGGCTTAT‑CCCATACGGCGACCCGGTCGGgcgc                                                                      >  2:8229576/1‑140 (MQ=14)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAACCCCCCCCCCCCCG‑CACCCCCCGGCAAAGGCGCACAAAAACGCCCCAGCGCCGGGCCCAAGGCATAT‑CCCATAACGCGACCGGGGCGGgcgc                                                                      >  2:5657304/1‑140 (MQ=9)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGgcgc                                                                      <  1:6050254/140‑1 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCCCTGCGCGGTGCCCTCGGCTTAT‑CCCTTTCGGCTACCGGGTCGGgcgc                                                                      >  2:8708185/1‑140 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAG‑CACCCCCCGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCTTAT‑ACCATACGGCTACCCGGTCGGgcgc                                                                      >  2:7664055/1‑140 (MQ=14)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAACCCGCCCCCCCCAG‑CCCCCCCCGGCACATGCGAAAAAAAAAGCCCCAGCGCCGTGCCCACCGCGTAA‑CCCATAACGCAACCCGGTCGGgcgc                                                                      >  2:5562345/1‑140 (MQ=9)
                                                                     gCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAACTCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGcgcg                                                                     <  2:8043521/140‑1 (MQ=255)
                                                                      cTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAACCCGCCGCCCCCAG‑CACCCCCCGGCACAGGCGAACAAAAAACCCCCGGCGCGGTGCCCACGGCTTAT‑ACCATAAGGCAACCCGGTCGGGcgcgc                                                                    >  2:4687820/1‑139 (MQ=9)
                                                                       ttCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAg                                                                   >  2:6896798/1‑140 (MQ=40)
                                                                         cATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCAAAC‑CCCCCCCCCCCAG‑CCCCCCCCGGGGCAGGCGACAAAAAAACCCCCAGCGCGGGGCCCACGGCGGAT‑CACGTACCGCGACCCGGGCGGGGGCGCggg                                                                 >  1:8576982/1‑139 (MQ=255)
                                                                           tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                               <  2:5630465/140‑1 (MQ=37)
                                                                             taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                             <  2:8872641/140‑1 (MQ=35)
                                                                             taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                             <  2:10922943/140‑1 (MQ=35)
                                                                             taATCACGGGCGCACGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGACCAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                             <  1:157321/140‑1 (MQ=255)
                                                                              aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                            <  2:3843397/140‑1 (MQ=34)
                                                                                tCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCAA‑CGCCGCCCCCCCCCG‑CCCCCCCCGGCGCTGGCGAAAAAAAACGCCGCGGCGCCGGGGCCACGGCGTAT‑CCCATAACGCGACCCGGTAGGGCGCCCGGGAACaaac                                                          >  2:7976637/1‑137 (MQ=255)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCCCCCCCCCCAA‑CACCCCCCCGCACTGGCGAAAAAAAAAGCCGCTGCGCGGTGGCCCCCGCGTAA‑ACCAAAACGCAACCCCGTCGGGCGCGAGGGAACaaac                                                          >  2:4632805/1‑137 (MQ=2)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAG‑CACCCCCGGGCACTGGCGAACAAAAACGCCGCTGCGCGGTGCCCTCGGCATAT‑CCCTTACGGCTAACCGGTAGGGCGCGAGGGAACAtac                                                          >  2:10696215/1‑138 (MQ=11)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCCCCGCCGCCCCCAG‑CACCCCCGGGCTCTGGCGAACAAAATACCCCCTGCCCGGTGCCCACGGCTTAT‑CCCATAAGGCTACCCGGTCGGGCGCGAGGTAAcctac                                                          >  2:7620224/1‑136 (MQ=11)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCCCCGCCCCCAG‑CACCCCCCGGCTCTGGCGAACAAAAACGCCGCTGCGCGGTGGCCTCGGCGTAT‑ACCATAAGGCAACCCGGTAGGGCGCGAGGTAACCTcc                                                          >  2:6359517/1‑140 (MQ=11)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAACCCCCCCCCCCAC‑CACCCCCCGGCGAATGCGAACAAAAAACCCCCGGCGCGGGGGCCCCGGCGTATACCCTAACGCAAACCGGG‑CGGGCGCGAGGTAACaaac                                                          >  2:4589530/1‑137 (MQ=2)
                                                                                    tGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCGG‑CACCCCCGGGCTCAGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCGTACCGCAACCGGGTAGGGGGCGCGGGAACATCCCAGt                                                      >  2:6781168/1‑140 (MQ=11)
                                                                                     ggCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCGCCGCCCCCCG‑CACCCCCCGGCTCTGGGGAAAAAAAAAACCGCTGCGCGGTGCCCACGGCGTAT‑AACGAAACGCAACCCGGTCGGGCGCGCGGTAACATACCAGTa                                                     >  2:1650920/1‑140 (MQ=2)
                                                                                            ggAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                                              <  2:5594884/140‑1 (MQ=32)
                                                                                             gAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                                             <  2:8267600/140‑1 (MQ=32)
                                                                                                cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  1:7216800/140‑1 (MQ=32)
                                                                                                cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  1:4334754/140‑1 (MQ=32)
                                                                                                      aGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                                     <  1:10646332/140‑1 (MQ=32)
                                                                                                             ggcCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc                              <  2:1496097/140‑1 (MQ=32)
                                                                                                              gcCAATCGCCGCCGCCACCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGACATCGGCTTAT‑ACCTTACGGCTACAGGGTAGGGCGCGATGTAACATCCATGTAAAACGCGCCCTCAGCCCACCTCCa                             >  2:1005420/1‑140 (MQ=11)
                                                                                                                  aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTACCATCCCTGTAAAACGCGCCCTCAGCCCACAGCCAtgtg                         >  1:2289632/1‑140 (MQ=17)
                                                                                                                  aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                         >  1:10451785/1‑140 (MQ=32)
                                                                                                                   tCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAATCGCGCCCTCAGCCCACATCCATGTgg                        >  1:1496661/1‑140 (MQ=17)
                                                                                                                    cgccgccgcCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                       >  1:7840540/1‑140 (MQ=32)
                                                                                                                       cgccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg                    >  1:756654/1‑140 (MQ=32)
                                                                                                                        gccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTCCCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGc                   >  2:9458973/1‑140 (MQ=17)
                                                                                                                         ccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAATCGCGCCCTCAGCCCACATCCATGTGGGCTGcc                  >  1:7336410/1‑140 (MQ=17)
                                                                                                                              cccTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGCGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGc             <  2:5161904/140‑1 (MQ=17)
                                                                                                                                cTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCt           <  2:6593009/140‑1 (MQ=32)
                                                                                                                                 tG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt          >  2:11021638/1‑140 (MQ=32)
                                                                                                                                    cACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCATGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCa        >  2:2845395/1‑140 (MQ=17)
                                                                                                                                        cccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGa    >  2:768556/1‑140 (MQ=32)
                                                                                                                                         ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGac   >  1:1269875/1‑139 (MQ=17)
                                                                                                                                         ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGaa   >  1:9881256/1‑140 (MQ=32)
                                                                                                                                          cGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAc  >  2:4825295/1‑140 (MQ=32)
                                                                                                                                           |                                                                                                                                           
TGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAT‑CCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAC  >  NZ_CP025268/2233111‑2233386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑