Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5590TA24.2% 569.4 / 55.4 290intergenic (+90/+62)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (63/141);  new base A (65/0);  total (147/143)
Fisher's exact test for biased strand distribution p-value = 1.05e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 307 total aligned reads displayed.

TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698
                                                                                                                                           |                                                                                                                                            
tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCNTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                              <  1:6163974/140‑1 (MQ=255)
tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                              <  1:5443636/140‑1 (MQ=255)
 ctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctc                                                                                                                                             >  2:1872143/1‑140 (MQ=255)
  tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAACcact                                                                                                                                            >  1:2332143/1‑140 (MQ=39)
    ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAACCCGCTcc                                                                                                                                          >  1:2629790/1‑140 (MQ=255)
       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACAGCCCGTACGGTCCCCTCACCCAAACCCGCAccccc                                                                                                                                       >  2:9872160/1‑139 (MQ=38)
       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                                       <  2:1651691/140‑1 (MQ=255)
         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                     <  2:10092184/140‑1 (MQ=255)
         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCGCACCCCaaa                                                                                                                                     >  1:10019623/1‑140 (MQ=255)
           actCGCATCACCTTAACTAGTGATAATAACAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                                   <  1:7823248/138‑1 (MQ=255)
                  gcACGTTAACTATTGATAATCAAAGACATTAACCGGAGGCCGGCTGAGATGCGCCAGCATCGCATGCGGCGATGCTGGCGCGTTGAATGTTAGATCCCGTACGGTGCCCTCTCCCTGACCCGCTCCCCAAAGGGGCgg‑gg                                                                                                                           >  2:730221/2‑140 (MQ=255)
                      cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCCAACCCCCAAAGGGCGA‑gggggc                                                                                                                       >  1:4270663/1‑138 (MQ=37)
                         aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCgg                                                                                                                    >  2:10441779/1‑139 (MQ=255)
                          aCTATTGATAATAAAAGACATTAACCNTAGGCCGNATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACCTTCCCCTCACCCTAACCCTCTCCCNAAAGGGGCGA‑GGGGACCGtt                                                                                                                   <  1:6102202/140‑1 (MQ=255)
                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCCCCCAAAACCACACCCCCCACGGGCGG‑GGGGGCCggg                                                                                                                   >  1:47939/1‑138 (MQ=25)
                             aTTGATAATAAAAGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTACCCGCACCCAAACCCACACCCCCAAGGGGGGA‑GGGGCCCGGTCAg                                                                                                                >  2:3379480/1‑140 (MQ=37)
                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCAACCCCAAAACCGCAACCCCCAGGGGCGG‑GGGGGCCCTTCAGt                                                                                                               >  1:802391/1‑140 (MQ=25)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTTCATCCCGTACGTTCCACTCACCCTAACCCTCTCCCCACAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                              >  2:6390276/1‑140 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACCGGGCGA‑GGGGACCGTTCAGTa                                                                                                              <  2:1178398/140‑1 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                              <  1:3228963/140‑1 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCTCACCCCAAAGGGGCGA‑GGGGACCGTTCAGTa                                                                                                              >  2:5325894/1‑140 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCAAACCCGCTACCCAAATGGGGCA‑TGGGGCCGTGCAgga                                                                                                              >  1:220662/1‑138 (MQ=37)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACCCCCCAACCCACACCCCCAACGGGCGC‑GGGGACCGGTCAGTa                                                                                                              >  1:9250285/1‑140 (MQ=37)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACAACCCAAAAGGGCGA‑GGGGGCCCGTCAGTa                                                                                                              >  1:11000756/1‑140 (MQ=37)
                               tGAGAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGA‑GGGGACCGGTCAGTa                                                                                                              >  2:4994485/1‑140 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTAc                                                                                                             <  1:2956581/140‑1 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCGCTCCCCAAAGGGGCGA‑GGGGACCGGTCAGTaa                                                                                                             >  2:2158486/1‑139 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTAc                                                                                                             >  1:7909150/1‑140 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAAACCACACCCCAAAGGGGCGA‑GGGGACCGTTAAGTAc                                                                                                             >  2:5953688/1‑140 (MQ=38)
                                    ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCCAAACCCCAAGGGGCGC‑GGGGGCCGGTCAGGACCGac                                                                                                         >  1:8190648/1‑139 (MQ=25)
                                      aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAAtt                                                                                                       <  1:6382031/140‑1 (MQ=255)
                                           aCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCGGAACCTCAACCCCAAGGGGCGA‑GGGGGACGGTGAGTAAGGGAttttggg                                                                                                  >  1:10804107/1‑136 (MQ=25)
                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGA‑GGGGACCGGTCAGTACGGTATTTAGCTTg                                                                                                >  2:8607930/1‑140 (MQ=255)
                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTAAACCTAACCCACTCCCCCAAGGGGCGA‑GGGGACCCTTCAGTACCGAAATTAGcatt                                                                                                >  2:443989/1‑137 (MQ=38)
                                              ttcaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:7967511/137‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:4353199/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:4810179/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:5953688/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:3868647/140‑1 (MQ=255)
                                              atAACCGTAGGCCGGATCAGACGCGCCATCAACGCAACCGGCGATGCTGGCTCGAAGAATTTTACCTCCCTTACGTTCCCCTCACCCTAACCCCCTCCCCAAAGGGGCGA‑GGGGACCGTTCATTACGGAATTTAGCttca                                                                                               <  2:11480496/139‑3 (MQ=25)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  1:4549473/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:3755688/140‑1 (MQ=255)
                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCGAAACCACACCCCAAAAGGGGGC‑GGGGGGCGGGCAGTACGGCAATTAGCTGGAAgggggt                                                                                        >  1:95215/1‑136 (MQ=19)
                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCCAAACCACAACCCCAAAGGGCGA‑GGGGGCCGGTCAGTACGGAAGTTAGCGTGTCGGGGCt                                                                                        >  1:2713807/1‑140 (MQ=25)
                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  1:2031144/140‑1 (MQ=255)
                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  1:8629974/140‑1 (MQ=255)
                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTAGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  2:2845338/140‑1 (MQ=255)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTGACCCTCTCCCCAAAGGGGCGA‑GGGGACCGGTCAGTGCGGAAATTATCATGACGCTGCTAACCAGc                                                                                 >  2:1417287/1‑140 (MQ=38)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCAAACCCACAACCCAAAAGGGCGAGGGGGACCG‑TAAGTTCGAGAATTAGCATGTAGGTGCTATACAGc                                                                                 >  1:8287683/1‑140 (MQ=255)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTCACCCTCTCCCCAAAGGGGCGA‑GGGGAACCTTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGc                                                                                 >  2:2510817/1‑140 (MQ=39)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTAGGTTACCCACACCCAATCCCTCTACCCAAAGGGGCGA‑GGGGACCGTTCAGTTCGGAATTTATGTTTTCGGTGCTATCCAGCa                                                                                >  2:8440538/1‑140 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                                <  2:2554864/140‑1 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTTGCTTGACGGTGCGATCCAGcc                                                                                >  1:10526344/1‑139 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                                <  2:40144/140‑1 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGAGGCTGGCGCGTTGAATTTTACATCCCGAACGTTCCCCTCACCCTCACCCGCTCCCCAAAGGGGCGA‑GGGGACCGGTCAGTAGGGCATTTAGCGTGACGGTGAGATACAGCa                                                                                >  2:7209131/1‑140 (MQ=37)
                                                                  aTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCAAAGGGGCGC‑GGGGACCCGTCAGTACGGAAATTAGCTTGACGGTGCTATACAGCATTaaa                                                                           >  2:4210155/1‑140 (MQ=255)
                                                                   tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                          <  1:9429881/140‑1 (MQ=255)
                                                                    gcgcCAGCATCGCCTCCGGCGATGCTGGCGCGTAGAATTTTACAACCCGAACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                         <  1:8911388/140‑1 (MQ=255)
                                                                    gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                         <  1:795870/140‑1 (MQ=255)
                                                                     cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                        <  1:11312216/140‑1 (MQ=255)
                                                                     cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                        <  2:3449721/140‑1 (MQ=255)
                                                                     cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                        <  1:1161548/140‑1 (MQ=255)
                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCCCCCAAACCCACTCCCCACAGGGGCGC‑GGGGGCCCGGCAGGACGGTATTTAGCTTGTCGGGGGTAACCCGGAGTAAATAcg                                                                       >  1:2998582/1‑139 (MQ=255)
                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                       <  1:11089709/140‑1 (MQ=255)
                                                                           catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                                  <  1:10077404/140‑1 (MQ=255)
                                                                           catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAACCCACTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGAAGGTGCTATACAGCATTAAATACTGGcca                                                                  >  2:9575466/1‑138 (MQ=39)
                                                                             tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                                <  1:6963410/140‑1 (MQ=255)
                                                                                catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTTAATAATGGCAATActc                                                             >  2:11031140/1‑140 (MQ=255)
                                                                                catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAACCCACACCCCAAAGGGGCGA‑GGGGACCGGTAAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAAAGGCAAAAcac                                                             >  2:4577416/1‑138 (MQ=255)
                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  1:11516550/140‑1 (MQ=255)
                                                                                           gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCACAGGGGCGA‑GGGGACCGTTAAGTACGGAATTTAGCTTGTCGGTGCTATACCGCATTAAATAAAGGCAATACCCTCACGCAACAt                                                  >  2:6255311/1‑140 (MQ=37)
                                                                                              ggCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                               <  1:2039524/140‑1 (MQ=255)
                                                                                                cgcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCCTTAAATACTGGCAATACTCTCACGCCAAATACgca                                             >  2:7395293/1‑140 (MQ=255)
                                                                                                  cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                           <  2:872544/140‑1 (MQ=255)
                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  2:11040174/140‑1 (MQ=255)
                                                                                                       aaTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGA‑GGGGACCGTACAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                                      <  2:8021052/140‑1 (MQ=255)
                                                                                                              cATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa                               <  2:3309930/140‑1 (MQ=255)
                                                                                                              cATCCCGTACGTTCCCCTCACCCAAACCCTCACCCCAAAGGGGCGT‑GGGGGCCGTTCAGTACGGAATTTAGCGTGACGGGGCTATCCAGCATTTAATACTGGCAAAACTCTCACGCAAAATCCGCAGCACCGTCTCTGCa                               >  2:9322358/1‑140 (MQ=38)
                                                                                                              cATCCCGTACGTTACCCACAACCGTACCCACTCCCCCAAGGGGCGA‑GGGGACCGTTCAGGACGGAATTTAGGGTGAAGGTGCTATCCAGCATTAAATAAGTGGAATACTCTCACGCCAAATCCGCAGCCCCGTCTCTTCa                               >  2:914958/1‑140 (MQ=255)
                                                                                                                 cccGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCCATACTCTCACGCAAAATCCCCAGCACCGTCTCTTAACCg                            >  2:6557802/1‑140 (MQ=255)
                                                                                                                   cGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGCATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCGCACGCAAAATCCGCAGCACCGTCTCTTCACCGCt                          >  2:10577045/1‑140 (MQ=255)
                                                                                                                   cGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCt                          <  1:1673096/140‑1 (MQ=255)
                                                                                                                     tACGTTCCCCTCACCCTAACCCGCTCCCCAAAGGGGCGC‑GGGGACCGGTCAGTACGGAATTTAGCGTGACGGGGCTATCCAGCCGTAAATACGGGCAATACTCACACGCAAAATCCGCAGCACCGGCGCGTCACCGCTGc                        >  2:2991892/1‑140 (MQ=37)
                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                     <  1:1762099/140‑1 (MQ=255)
                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTGAGCTTGACGGTGCGATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                     <  2:7074910/140‑1 (MQ=255)
                                                                                                                         ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt                    <  2:2692455/140‑1 (MQ=255)
                                                                                                                             ccTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg                <  1:1476132/140‑1 (MQ=255)
                                                                                                                                  cccTAACCCTCAACCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAATGGCAAAACTAACACGCAAAAAACGCAGCACCCTCTCTTCACCGCAGCGATTCGGGTTAAt           >  2:5823250/1‑140 (MQ=38)
                                                                                                                                  cccAAAACCACTCCCCAAATGGGCGA‑GGGGACCGTTCAGTACCGAAATTATCTTTACGGTGCTATACAGCATTAAATAATGGCAATACACTAAAGCAAAATCCGCAGCACCGTCTATTCACCCCAGCGATTCGGGTTAAt           >  2:178097/1‑140 (MQ=255)
                                                                                                                                  accaaaCCCACTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTTACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAAGCAAAATCCGCAGCACCCTCTCTTAACCGCAGCGATTCGGGTTAAt           >  2:10964584/5‑140 (MQ=255)
                                                                                                                                   ccTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg          >  2:5203960/1‑140 (MQ=255)
                                                                                                                                      caCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       <  1:4033669/139‑1 (MQ=255)
                                                                                                                                      aaCCCTCTCCCCAAAGTGGCGA‑GGGTACCGTTCAGTACGGAATTTAGCGTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTATTGCGa       >  1:3034428/1‑140 (MQ=255)
                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGACTTTAGCTTGACGGGGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACTGCTGCGATTCGGGTTAATGCGa       >  2:908926/1‑140 (MQ=255)
                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:6820377/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      >  1:9798267/1‑140 (MQ=255)
                                                                                                                                           tctcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:4153074/1‑140 (MQ=255)
                                                                                                                                           tctcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:7169071/1‑140 (MQ=255)
                                                                                                                                           tctcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  2:5655455/1‑140 (MQ=255)
                                                                                                                                           tctcCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  2:11249979/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGA‑GGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑