BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BX571857	1,868,342	G→A	G129D (GGT→GAT)	SAS1720 →	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BX571857	1,868,342	0	G	A	100.0%	745.5 / NA	166	G129D (GGT→GAT)	SAS1720	putative membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (72/94); total (72/94)

Only 100 of 166 total aligned reads displayed.

TCAGTTGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGGTAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGCTA  >  BX571857/1868248‑1868434

    ttGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaaga                                                                                        >  1:1751518/1‑97 (MQ=255)

    ttGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaaga                                                                                        >  1:1156939/1‑97 (MQ=255)

    ttGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaaga                                                                                        <  2:2663227/97‑1 (MQ=255)

    ttGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaaga                                                                                        >  1:1566036/1‑97 (MQ=255)

    ttGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaaga                                                                                        <  2:668550/97‑1 (MQ=255)

     tGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaagaa                                                                                       >  2:559669/1‑97 (MQ=255)

      gAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaagaag                                                                                      <  2:248271/97‑1 (MQ=255)

       aaaCTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGc                                                                                     <  2:2516267/97‑1 (MQ=255)

         aCTATGAAATCAGTTGGTCCATCTATCGCGCCAACCAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGt                                                                                   >  2:1936575/1‑97 (MQ=255)

         aCTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGt                                                                                   >  2:1915585/1‑97 (MQ=255)

          cTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGtt                                                                                  <  2:729680/97‑1 (MQ=255)

          cTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGtt                                                                                  <  1:1906127/97‑1 (MQ=255)

          cTATGAAAGCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGtt                                                                                  <  1:1338874/97‑1 (MQ=255)

           tATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTa                                                                                 <  1:956036/97‑1 (MQ=255)

           tATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTa                                                                                 >  2:823553/1‑97 (MQ=255)

            aTGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTaa                                                                                <  1:1963751/97‑1 (MQ=255)

             tGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAAc                                                                               <  1:802499/97‑1 (MQ=255)

              gAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACa                                                                              <  2:1694750/97‑1 (MQ=255)

              gAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACa                                                                              <  2:2202198/97‑1 (MQ=255)

                 aTCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAAtt                                                                           >  1:2274570/1‑97 (MQ=255)

                 aTCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAAtt                                                                           <  1:2613363/97‑1 (MQ=255)

                   cAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTCACAATTGa                                                                         >  1:2647857/1‑97 (MQ=255)

                   cAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGa                                                                         >  2:1631019/1‑97 (MQ=255)

                   cAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGGTGGTGATGTGACGATTAACAACACAGATAAAGACGCGTTCACAATTgg                                                                         >  2:387703/1‑96 (MQ=255)

                    aGTTGGTCCATCTATCGCGCCAACGAATGCTAAAGGTACATGTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGAt                                                                        <  2:2263422/97‑1 (MQ=255)

                    aGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGAt                                                                        >  1:1282263/1‑97 (MQ=255)

                    aGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGAt                                                                        >  1:1282246/1‑97 (MQ=255)

                     gTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATa                                                                       >  2:2240453/1‑97 (MQ=255)

                      ttGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAg                                                                      >  1:507265/1‑97 (MQ=255)

                      ttGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAg                                                                      >  1:497787/1‑97 (MQ=255)

                      ttGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAg                                                                      <  2:205888/97‑1 (MQ=255)

                       tGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGt                                                                     >  1:2037505/1‑97 (MQ=255)

                        ggTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGtt                                                                    >  2:1855589/1‑97 (MQ=255)

                          tCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCa                                                                  >  2:2110489/1‑97 (MQ=255)

                           ccATCTATCGCGCCAACAAATGCTAAAGGGACATTTGTCGTGGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCaa                                                                 <  2:2675975/97‑1 (MQ=255)

                            cATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAAt                                                                <  1:2213092/97‑1 (MQ=255)

                            cATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAAt                                                                <  1:1612946/97‑1 (MQ=255)

                            cATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGAGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGGTAGGTCAAt                                                                >  1:2033829/1‑97 (MQ=255)

                               cTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGtt                                                             <  2:641503/97‑1 (MQ=255)

                                 aTCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTa                                                           <  2:1191384/97‑1 (MQ=255)

                                 aTCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTa                                                           <  1:1692517/97‑1 (MQ=255)

                                  tCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTaa                                                          <  2:775399/97‑1 (MQ=255)

                                   cgcgCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTaaa                                                         <  2:1358208/97‑1 (MQ=255)

                                     cgcCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACt                                                       <  2:436333/97‑1 (MQ=255)

                                        caacaaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGaa                                                    <  1:1810035/97‑1 (MQ=255)

                                        caacaaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGaa                                                    <  1:2392868/97‑1 (MQ=255)

                                         aacaaATGCTAAAGGTACATTTGTCGTTGCTGATGTTACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGaaa                                                   <  2:596943/97‑1 (MQ=255)

                                         aacaaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGaaa                                                   <  2:685114/97‑1 (MQ=255)

                                         aacaaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGaaa                                                   <  2:202697/97‑1 (MQ=255)

                                           caaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATc                                                 >  1:903975/1‑97 (MQ=255)

                                           caaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATc                                                 <  1:1752249/97‑1 (MQ=255)

                                           caaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATc                                                 <  1:1895178/97‑1 (MQ=255)

                                           caaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATc                                                 <  1:1987666/97‑1 (MQ=255)

                                           caaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATc                                                 <  1:1409076/97‑1 (MQ=255)

                                             aaTGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTg                                               >  1:1273011/1‑97 (MQ=255)

                                             aaTGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTg                                               >  2:1919650/1‑97 (MQ=255)

                                               tGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGt                                             >  2:1770207/1‑97 (MQ=255)

                                                 cTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGa                                           >  1:1482627/1‑97 (MQ=255)

                                                    aaGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATaa                                        <  2:1515263/97‑1 (MQ=255)

                                                      ggTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATaaaa                                      >  2:2309803/1‑97 (MQ=255)

                                                      ggTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATaaaa                                      >  2:2475374/1‑97 (MQ=255)

                                                      ggTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATaaaa                                      <  1:825013/97‑1 (MQ=255)

                                                      ggTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATaaaa                                      >  2:1347543/1‑97 (MQ=255)

                                                       gTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAAc                                     >  1:2182826/1‑97 (MQ=255)

                                                       gTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAAc                                     >  1:110767/1‑97 (MQ=255)

                                                        tACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACa                                    <  1:66348/97‑1 (MQ=255)

                                                         aCATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACAt                                   <  1:477631/97‑1 (MQ=255)

                                                         aCATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACAt                                   >  2:2603924/1‑97 (MQ=255)

                                                         aCATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACAt                                   <  2:1442984/97‑1 (MQ=255)

                                                          cATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACAtt                                  >  2:2439617/1‑97 (MQ=255)

                                                            tttGTCGTTGCTGATGTGACTATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTg                                <  2:713675/97‑1 (MQ=255)

                                                            tttGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTg                                >  1:1021737/1‑97 (MQ=255)

                                                             ttGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGa                               <  1:1756986/97‑1 (MQ=255)

                                                              tGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGaa                              >  2:2636993/1‑97 (MQ=255)

                                                              tGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGaa                              >  2:2097862/1‑97 (MQ=255)

                                                              tGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGaa                              <  2:655272/97‑1 (MQ=255)

                                                                     gCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGata                       >  1:1509422/1‑97 (MQ=255)

                                                                     gCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGata                       >  1:2342898/1‑97 (MQ=255)

                                                                        gATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGataata                    >  1:446802/1‑97 (MQ=255)

                                                                          tgtgACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACa                  >  2:8417/1‑97 (MQ=255)

                                                                          tgtgACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACa                  <  2:1899287/97‑1 (MQ=255)

                                                                          tgtgACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACa                  >  2:1474646/1‑97 (MQ=255)

                                                                              aCGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCt              >  2:1550366/1‑97 (MQ=255)

                                                                               cGATTAAAGACATAGATAAAGAAGCGTGAACACTTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATCCAGGCTc             <  2:2300035/97‑1 (MQ=255)

                                                                               cGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTc             <  1:1030004/97‑1 (MQ=255)

                                                                                gATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCa            <  2:885666/97‑1 (MQ=255)

                                                                                gATTAAAAACAAAGACAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCa            <  2:395319/97‑1 (MQ=255)

                                                                                  ttAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAAt          <  2:102667/97‑1 (MQ=255)

                                                                                   tAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATg         <  2:205518/97‑1 (MQ=255)

                                                                                   tAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATg         >  1:589760/1‑97 (MQ=255)

                                                                                   tAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATg         <  1:531065/97‑1 (MQ=255)

                                                                                    aaaaaCAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGt        >  2:1842265/1‑97 (MQ=255)

                                                                                      aaaCAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCt      <  2:1291963/97‑1 (MQ=255)

                                                                                      aaaCAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCt      <  2:1100782/97‑1 (MQ=255)

                                                                                      aaaCAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCt      >  1:715617/1‑97 (MQ=255)

                                                                                        aCAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGc    >  2:1500322/1‑97 (MQ=255)

                                                                                         cAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGCt   <  2:363130/97‑1 (MQ=255)

                                                                                         cAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGCt   <  1:195121/97‑1 (MQ=255)

                                                                                         cAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACAATTGAAGCAGATAATACAGGCTCAATGACTGCt   >  2:262042/1‑97 (MQ=255)

                                                                                          aaaGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACGGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGCTa  <  2:2221018/97‑1 (MQ=255)

TCAGTTGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGGTAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGCTA  >  BX571857/1868248‑1868434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`