Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 605,157 T→C 5.4% D1007D (GAT→GAC SAS0521 → bone sialoprotein‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857605,1570TC 5.4% 1283.2 / 12.6 354D1007D (GAT→GACSAS0521bone sialoprotein‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (143/184);  new base C (15/10);  total (159/195)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

ACTCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACA  >  BX571857/605060‑605254
                                                                                                 |                                                                                                 
actcagactcagATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                                                                   <  1:2294453/98‑1 (MQ=255)
 ctcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                  >  2:567820/1‑98 (MQ=255)
 ctcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                  >  1:472012/1‑98 (MQ=255)
  tcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTc                                                                                                 <  2:1980968/98‑1 (MQ=255)
  tcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTc                                                                                                 <  1:2408387/98‑1 (MQ=255)
    agactcagcTAGTGATTCAGATTCAGCTAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAg                                                                                               <  1:2652482/98‑1 (MQ=255)
    agactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAg                                                                                               <  2:1152912/98‑1 (MQ=40)
    agactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAg                                                                                               <  2:2441109/98‑1 (MQ=255)
      actcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                                                             <  2:622988/98‑1 (MQ=255)
       ctcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                                                            <  1:1981815/98‑1 (MQ=255)
        tcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                                                           <  2:1543790/98‑1 (MQ=255)
          agaTATTGATTCCCATTCAGACAGCGACTCAGACGCAGATAGCCACTCGGAGTCGGACAGCGATTCAGACTCCGATAGCGACTCAGATTCAGACAGCg                                                                                         <  2:2105951/98‑1 (MQ=11)
           gaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                        <  1:1735934/98‑1 (MQ=255)
           gaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                        <  1:252209/98‑1 (MQ=40)
           gaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                                                                                        >  2:229534/1‑98 (MQ=12)
            aTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                       <  2:1035294/98‑1 (MQ=40)
            aTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                       <  1:437354/98‑1 (MQ=255)
            aTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                       <  2:1583296/98‑1 (MQ=40)
             tAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                      <  1:1079771/98‑1 (MQ=255)
              aGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                     >  2:2157605/1‑98 (MQ=255)
                tGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                   >  1:971716/1‑98 (MQ=40)
                tGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                   <  1:2170317/98‑1 (MQ=255)
                tGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                   >  2:398302/1‑98 (MQ=255)
                tGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAGTCAGACAGCGATTCAg                                                                                   >  2:1693768/1‑98 (MQ=255)
                 gattcagattcaGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                  <  1:292609/98‑1 (MQ=255)
                  attcagattcagATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                 <  2:2337756/98‑1 (MQ=255)
                   ttcagattcagaTAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACt                                                                                <  1:55315/98‑1 (MQ=255)
                   ttcagattcagaTAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACt                                                                                <  2:3251314/98‑1 (MQ=255)
                      agattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg                                                                             <  1:304659/98‑1 (MQ=255)
                       gattctgatAGCGATTCAGACTCAGATCGCGTCTCAGAATCGGTCGGCGATCCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGa                                                                            <  2:3065365/98‑1 (MQ=11)
                        attcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                           >  1:1111079/1‑98 (MQ=255)
                        attcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACACAGATACAGACAGCGAACCAGACTCAGAt                                                                           >  2:2491023/1‑98 (MQ=14)
                         ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATa                                                                          <  2:180759/98‑1 (MQ=255)
                          tcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                         <  1:245206/98‑1 (MQ=255)
                            agatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                       <  1:1800170/98‑1 (MQ=255)
                            agatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATGCAGACAGCGATTCAGACTCAGATAGCg                                                                       <  1:1311580/98‑1 (MQ=255)
                             gatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGa                                                                      >  1:1357996/1‑98 (MQ=255)
                              atAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGAc                                                                     >  1:2788236/1‑98 (MQ=255)
                              atAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGAc                                                                     >  2:814846/1‑98 (MQ=255)
                              atAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGAc                                                                     <  2:2595495/98‑1 (MQ=255)
                              atAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGAc                                                                     >  1:254564/1‑98 (MQ=21)
                              atAGCGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGat                                                                     >  2:553130/1‑97 (MQ=12)
                               tAGCGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACt                                                                    <  2:2599950/98‑1 (MQ=255)
                                ggCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTc                                                                   <  2:1884817/97‑1 (MQ=255)
                                aGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTc                                                                   >  2:2727381/1‑98 (MQ=255)
                                 gCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCa                                                                  >  2:1192815/1‑98 (MQ=255)
                                  cGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAg                                                                 >  2:1462326/1‑98 (MQ=255)
                                  cGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAg                                                                 <  1:1038597/98‑1 (MQ=255)
                                  cGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAg                                                                 <  2:2869781/98‑1 (MQ=40)
                                   gactcagactcaGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGa                                                                <  2:484587/98‑1 (MQ=255)
                                    actcagactcagATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                               >  1:2952189/1‑98 (MQ=255)
                                        agactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAg                                                           <  1:3281035/98‑1 (MQ=255)
                                          actcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                         <  1:867951/98‑1 (MQ=255)
                                           ctcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                        >  1:749813/1‑98 (MQ=255)
                                           ctcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                        >  2:105831/1‑98 (MQ=255)
                                            tcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                       >  1:1634921/1‑98 (MQ=255)
                                              agaCAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTg                                                     <  2:321386/98‑1 (MQ=255)
                                               gaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTga                                                    >  1:2235251/1‑98 (MQ=255)
                                               gaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                    >  2:1245532/1‑98 (MQ=255)
                                                aTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgac                                                   >  1:1200445/1‑98 (MQ=255)
                                                 tAGCGACTCAGCTTCGGTCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCACATAGCGACTCAGATTCACACAGTgact                                                  <  2:1348024/98‑1 (MQ=14)
                                                 tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgact                                                  >  1:899251/1‑98 (MQ=255)
                                                 tAGCGACTCAGATTCCGACAGCGAGTGAGACTCAGATAGTGATTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgact                                                  <  2:2361650/98‑1 (MQ=11)
                                                  cgCGACTCAGATTCGGACAGCGATGCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactc                                                 <  2:37707/97‑1 (MQ=255)
                                                  aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactc                                                 <  2:929870/98‑1 (MQ=255)
                                                   gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactca                                                >  1:203861/1‑98 (MQ=255)
                                                    cGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                               <  1:2686387/98‑1 (MQ=255)
                                                     gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcaga                                              <  1:2319531/98‑1 (MQ=255)
                                                           gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGActcagactcaga                                        >  2:2042720/1‑98 (MQ=40)
                                                             ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATa                                      >  1:168351/1‑98 (MQ=255)
                                                             ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATa                                      >  1:1151812/1‑98 (MQ=255)
                                                             ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGAGTCAGACAGCGATTCAGACTCAGATAGCGCCTCAGATTCAGACTGTGACTCAGACTCAGATa                                      <  2:311305/98‑1 (MQ=255)
                                                              tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGCTAg                                     >  2:675526/1‑98 (MQ=255)
                                                              tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAg                                     <  1:1555471/98‑1 (MQ=255)
                                                              tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGACAGTGACTCAGACTCAGATAg                                     >  2:2524782/1‑98 (MQ=255)
                                                                ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCg                                   >  2:2675171/1‑98 (MQ=255)
                                                                      cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                             >  2:1098205/1‑98 (MQ=255)
                                                                      cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                             >  2:341753/1‑98 (MQ=255)
                                                                          tCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagactc                         <  2:3273967/98‑1 (MQ=255)
                                                                             gACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGACAGTGACGCAGACTCAGATAGCGActcagactcaga                      >  2:2650686/1‑98 (MQ=255)
                                                                              ccTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACtcagactcagac                     <  2:1941347/97‑1 (MQ=255)
                                                                              aCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACtcagactcagac                     >  1:1506339/1‑98 (MQ=255)
                                                                              aCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACtcagactcagac                     >  2:1265237/1‑98 (MQ=255)
                                                                                  aGATAGCGACTCAGATTCAGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                 <  2:1559537/98‑1 (MQ=255)
                                                                                     tAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgact              <  1:718268/98‑1 (MQ=255)
                                                                                      aGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactc             >  1:260381/1‑98 (MQ=255)
                                                                                      aGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactc             <  2:2764835/98‑1 (MQ=255)
                                                                                      aGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactc             <  2:748751/98‑1 (MQ=255)
                                                                                       gCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactca            <  2:1406229/98‑1 (MQ=255)
                                                                                         gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcaga          <  1:1180649/98‑1 (MQ=255)
                                                                                          aCTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagac         >  1:3109853/1‑98 (MQ=255)
                                                                                          aCTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagac         >  2:1657985/1‑98 (MQ=255)
                                                                                           cTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact        <  2:2229619/98‑1 (MQ=255)
                                                                                           cTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact        >  1:2877393/1‑98 (MQ=255)
                                                                                           cTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact        >  1:3073442/1‑98 (MQ=255)
                                                                                            tcagactcagacAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCgacgcagagtc       >  2:1911764/1‑98 (MQ=11)
                                                                                            tCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc       >  2:2205451/1‑98 (MQ=255)
                                                                                               gATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGActcagactcaga    <  1:2733989/98‑1 (MQ=255)
                                                                                                aTTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac   <  1:613473/98‑1 (MQ=255)
                                                                                                 ttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACa  >  2:29188/1‑98 (MQ=255)
                                                                                                 |                                                                                                 
ACTCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACA  >  BX571857/605060‑605254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: