Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BX571857	1,868,342	G→A	100%	G129D (GGT→GAT)	SAS1720 →	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BX571857	1,868,342	0	G	A	100.0%	115.6 / NA	28	G129D (GGT→GAT)	SAS1720	putative membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/19);  total (9/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTTGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGGTAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGCT  >  BX571857/1868250‑1868433                                                                                             |                                                                                            aGTTGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATaaa                                                                                         <  2:79821/97‑1 (MQ=255)   ttGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaaga                                                                                       >  2:33163/1‑97 (MQ=255)     gAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaagaag                                                                                     <  1:65823/97‑1 (MQ=255)      gagcTATGAAATCAGTTGGTCCAGCTAGCGCGCCAACAAATGCTAAAGGTACATTCGTCGTTGCTGATGTGACGATTAAAAACAAAGATTAAGAGGc                                                                                    <  2:382430/94‑1 (MQ=255)            tGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAAc                                                                              >  1:118737/1‑97 (MQ=255)            tGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAAc                                                                              <  2:379566/97‑1 (MQ=255)                  cAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGa                                                                        <  1:38943/97‑1 (MQ=255)                   aGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGAt                                                                       >  1:281703/1‑97 (MQ=255)                       ggTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGtt                                                                   >  1:36362/1‑97 (MQ=255)                             tCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGt                                                             <  2:96561/97‑1 (MQ=255)                              cTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGtt                                                            <  2:117304/97‑1 (MQ=255)                                 tCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTaa                                                         <  1:101545/97‑1 (MQ=255)                                 tCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTaa                                                         <  1:383063/97‑1 (MQ=255)                                 tCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTaa                                                         <  1:101351/97‑1 (MQ=255)                                      ccAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGa                                                    <  1:72557/97‑1 (MQ=255)                                        aacaaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGaaa                                                  <  2:187080/97‑1 (MQ=255)                                        aacaaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGaaa                                                  >  1:224978/1‑97 (MQ=255)                                                      gTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAAc                                    <  2:300512/97‑1 (MQ=255)                                                       tACCTTTGTCTTTGCTGATTTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACa                                   <  2:41637/97‑1 (MQ=255)                                                           tttGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTg                               >  1:67253/1‑97 (MQ=255)                                                           tttGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTg                               <  1:379715/97‑1 (MQ=255)                                                               tCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGc                           >  2:316572/1‑97 (MQ=255)                                                                   tGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGat                       <  2:239056/97‑1 (MQ=255)                                                                        aTGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATAc                  <  1:23325/97‑1 (MQ=255)                                                                               gATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCa           <  1:78022/97‑1 (MQ=255)                                                                                  tAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATg        >  2:285934/1‑97 (MQ=255)                                                                                        cAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGCt  <  2:2923/97‑1 (MQ=255)                                                                                        cAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGCGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGCt  >  1:178667/1‑97 (MQ=255)                                                                                             |                                                                                            AGTTGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGGTAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCTGCT  >  BX571857/1868250‑1868433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑