Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 605,157 T→C 6.4% D1007D (GAT→GAC SAS0521 → bone sialoprotein‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857605,1570TC 6.4% 1461.7 / 19.0 406D1007D (GAT→GACSAS0521bone sialoprotein‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (192/179);  new base C (27/6);  total (219/187)
Fisher's exact test for biased strand distribution p-value = 8.42e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 426 total aligned reads displayed.

CTCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGAC  >  BX571857/605061‑605253
                                                                                                |                                                                                                
ctcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                                                                  <  1:3506152/97‑1 (MQ=255)
ctcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                                                                  >  1:2055362/1‑97 (MQ=255)
 tcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                 >  1:662608/1‑97 (MQ=255)
  cagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTc                                                                                                >  1:969384/1‑97 (MQ=255)
   agactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                               >  1:3115892/1‑97 (MQ=255)
   agactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                               <  1:4455142/97‑1 (MQ=255)
    gactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAg                                                                                              <  2:1928565/97‑1 (MQ=40)
     actcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                             <  2:3464506/97‑1 (MQ=40)
     actcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                             <  2:2143207/97‑1 (MQ=255)
      ctcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                                                            >  1:119397/1‑97 (MQ=255)
       tcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                                                           <  1:2725992/97‑1 (MQ=255)
         agaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGc                                                                                         <  1:3052431/97‑1 (MQ=255)
          gaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCg                                                                                        <  1:2310856/97‑1 (MQ=40)
             aGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                     <  2:4565052/97‑1 (MQ=40)
             aGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                     <  1:1497841/97‑1 (MQ=40)
             aGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                     <  1:2510681/97‑1 (MQ=39)
              gTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATGGCGACTCAGATTCAGACAGCGATTc                                                                                    <  1:906522/97‑1 (MQ=255)
              gTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                    <  2:829924/97‑1 (MQ=255)
               tGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACCGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                                                                   <  1:3287041/97‑1 (MQ=255)
               tGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                                                                   <  2:2670220/97‑1 (MQ=255)
               tGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                                                                   <  1:4278815/97‑1 (MQ=255)
               tGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                                                                   <  1:1164326/97‑1 (MQ=255)
                gattcagattcaGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGGGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                  <  2:1705934/97‑1 (MQ=255)
                gattcagattcaGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                  <  2:4416081/97‑1 (MQ=255)
                  ttcagattcagaTAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                <  2:2261522/97‑1 (MQ=255)
                   tcagattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACt                                                                               <  2:4174059/97‑1 (MQ=255)
                   tcagattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACt                                                                               >  1:104765/1‑97 (MQ=255)
                     agattcagatAGCGACTCAGCCTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                             <  2:309867/97‑1 (MQ=255)
                     agattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                             <  1:3755254/97‑1 (MQ=255)
                       attcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGa                                                                           >  2:4447932/1‑97 (MQ=18)
                        ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGAt                                                                          >  2:8874/1‑97 (MQ=21)
                        ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAATCAGACTCAGAt                                                                          >  2:1323434/1‑97 (MQ=255)
                          cagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAg                                                                        >  2:2511783/1‑97 (MQ=21)
                            gatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                      <  1:4585094/97‑1 (MQ=255)
                            gatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                      <  1:4113197/97‑1 (MQ=255)
                             acaGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGa                                                                     <  2:3387927/95‑1 (MQ=255)
                              tAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGAc                                                                    >  2:4096768/1‑97 (MQ=255)
                              tAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGAc                                                                    >  1:1274624/1‑97 (MQ=255)
                               aGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACt                                                                   <  1:211199/97‑1 (MQ=255)
                                gCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTc                                                                  >  1:565342/1‑97 (MQ=255)
                                  cactcagactcagATAGCGACTCAGATGCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAg                                                                <  1:4479886/96‑1 (MQ=255)
                                     tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                             >  2:760557/1‑97 (MQ=255)
                                      cagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTc                                                            >  2:2621420/1‑97 (MQ=255)
                                       agactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGc                                                                       >  2:4334904/1‑85 (MQ=39)
                                              gaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTg                                                    <  2:3533713/97‑1 (MQ=255)
                                              gaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTg                                                    <  1:2821458/97‑1 (MQ=255)
                                               aTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTga                                                   >  2:1173578/1‑97 (MQ=255)
                                               aTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTga                                                   >  1:4295679/1‑97 (MQ=255)
                                                tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgac                                                  <  1:3403235/97‑1 (MQ=255)
                                                tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgac                                                  >  2:1062384/1‑97 (MQ=255)
                                                tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgac                                                  <  1:3925408/97‑1 (MQ=255)
                                                 aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgact                                                 >  1:1952399/1‑97 (MQ=255)
                                                  gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactc                                                <  1:14719/97‑1 (MQ=255)
                                                    gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              <  2:3964651/97‑1 (MQ=255)
                                                    gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              >  1:3488961/1‑97 (MQ=255)
                                                    gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag                                              >  2:2916940/1‑97 (MQ=255)
                                                      cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagac                                            >  2:4630744/1‑97 (MQ=255)
                                                      cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagac                                            >  2:3298498/1‑97 (MQ=255)
                                                       acAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagact                                           <  2:4462403/96‑1 (MQ=255)
                                                         aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactca                                         >  1:3251225/1‑97 (MQ=255)
                                                         aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactca                                         <  1:2386974/97‑1 (MQ=40)
                                                           aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGActcagactcaga                                       <  1:3030079/97‑1 (MQ=255)
                                                             tccgACCGCGATCCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTGAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATa                                     <  2:2816827/94‑1 (MQ=14)
                                                             tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGActcagactcagaca                                     >  2:3672694/1‑95 (MQ=255)
                                                             tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATa                                     >  1:3592022/1‑97 (MQ=255)
                                                                gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCg                                  <  2:1437347/97‑1 (MQ=255)
                                                                  cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgac                                >  1:1847369/1‑97 (MQ=255)
                                                                   aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgact                               >  1:2633432/1‑97 (MQ=255)
                                                                     cGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAGAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactca                             <  2:2558127/97‑1 (MQ=255)
                                                                     cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactca                             >  1:1630675/1‑97 (MQ=255)
                                                                      gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                            >  1:3356208/1‑97 (MQ=255)
                                                                      gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                            >  1:3550056/1‑97 (MQ=255)
                                                                      gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                            <  1:387255/97‑1 (MQ=255)
                                                                      gATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATTGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                            <  2:3765051/97‑1 (MQ=255)
                                                                        ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagac                          >  1:4644730/1‑97 (MQ=255)
                                                                         tCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagact                         >  2:2387196/1‑97 (MQ=255)
                                                                         tCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagact                         >  1:72314/1‑97 (MQ=255)
                                                                           aGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagactca                       <  1:289302/97‑1 (MQ=255)
                                                                            gccTCAGACAGTGACTCAGCTTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGactcagactcag                      <  2:517888/95‑1 (MQ=11)
                                                                             acacAGATAGCGACTCAGATTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGAGTCAGACAGAGACACAGACTCAGATAGCGacgcggactcagc                     >  2:1906699/4‑96 (MQ=14)
                                                                             aCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGActcagactcaga                     <  2:1503944/97‑1 (MQ=255)
                                                                             aCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGActcagactcaga                     >  1:4185107/1‑97 (MQ=255)
                                                                              cTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACtcagactcagac                    >  1:786979/1‑97 (MQ=255)
                                                                                cAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAg                  >  1:2454077/1‑97 (MQ=255)
                                                                                 aGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 <  1:1424103/97‑1 (MQ=255)
                                                                                  gATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                <  2:2326200/97‑1 (MQ=255)
                                                                                  gATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                <  2:1558263/97‑1 (MQ=255)
                                                                                   aTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTga               >  2:2046213/1‑97 (MQ=255)
                                                                                     aGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGGgact             >  2:1814941/1‑97 (MQ=255)
                                                                                      gCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactc            >  1:2750716/1‑97 (MQ=255)
                                                                                      gCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactc            >  1:4545568/1‑97 (MQ=255)
                                                                                        gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag          >  1:2039946/1‑97 (MQ=255)
                                                                                           tcagactcagacAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCgactcagact       >  1:2201504/1‑97 (MQ=255)
                                                                                            cAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc      >  2:3636277/1‑97 (MQ=255)
                                                                                            cAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc      >  1:4019307/1‑97 (MQ=255)
                                                                                              gATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGactcagactcag    <  1:1558139/97‑1 (MQ=255)
                                                                                               aTTCAGACCGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGActcagactcaga   >  1:173878/1‑97 (MQ=255)
                                                                                                ttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac  >  2:4273164/1‑97 (MQ=255)
                                                                                                ttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac  >  2:1358153/1‑97 (MQ=255)
                                                                                                ttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac  <  1:2670910/97‑1 (MQ=255)
                                                                                                |                                                                                                
CTCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGAC  >  BX571857/605061‑605253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: