mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 547,694 | A→G | E38E (GAA→GAG) | Y75_RS02655 → | hypothetical protein |
| RA | 547,835 | +G | coding (255/261 nt) | Y75_RS02655 → | hypothetical protein |
| RA | 556,778 | A→C | F63V (TTC→GTC) | folD ← | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD |
| RA | 556,858 | A→T | L36Q (CTG→CAG) | folD ← | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD |
| MC JC | 654,214 | Δ1,199 bp | Y75_RS03225–[dcuC] | Y75_RS03225, [dcuC] | |
| RA | 987,574 | G→T | intergenic (‑170/+433) | ompF ← / ← asnS | porin OmpF/asparagine‑‑tRNA ligase |
| RA | 1,093,686 | T→C | V130A (GTA→GCA) | Y75_RS05355 → | GGDEF domain‑containing protein |
| RA | 1,197,797 | C→T | H366H (CAC→CAT) | icd → | NADP‑dependent isocitrate dehydrogenase |
| RA | 1,197,809 | C→T | T370T (ACC→ACT) | icd → | NADP‑dependent isocitrate dehydrogenase |
| MC JC | 1,301,041 | Δ1,336 bp | Y75_RS06530–Y75_RS06530 | Y75_RS06530, Y75_RS06530 | |
| RA | 1,372,264 | A→G | E112G (GAG→GGG) | Y75_RS06905 → | sugar phosphorylase |
| RA | 1,519,222 | G→T | V40V (GTG→GTT) | Y75_RS07640 → | orphan toxin OrtT |
| RA | 1,669,601 | Δ1 bp | coding (678/1221 nt) | mlc ← | DNA‑binding transcriptional repressor Mlc |
| RA | 1,979,957 | G→T | intergenic (‑46/+398) | flhD ← / ← Y75_RS09980 | flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase |
| RA | 2,005,401 | C→T | E115K (GAA→AAA) | fliC ← | H48 family flagellin FliC |
| MC JC | 2,176,983 | Δ1,199 bp | Y75_RS10980–[gatA] | Y75_RS10980, [gatA] | |
| RA | 2,245,455 | A→G | S283P (TCG→CCG) | Y75_RS11290 ← | DUF418 family protein |
| MC JC | 2,320,795 | Δ1,336 bp | Y75_RS11650–[rcsC] | Y75_RS11650, Y75_RS11650, [rcsC] | |
| RA | 2,866,111 | A→G | pseudogene (97/993 nt) | rpoS ← | RNA polymerase sigma factor RpoS |
| MC JC | 3,261,134 | Δ1,199 bp | Y75_RS16205–[tdcD] | Y75_RS16205, [tdcD] | |
| RA | 3,268,825 | A→G | T186A (ACA→GCA) | Y75_RS16240 → | YhaC family protein |
| MC JC | 3,742,936 | Δ1,336 bp | Y75_RS18560–Y75_RS18560 | Y75_RS18560, Y75_RS18560 | |
| MC JC | 3,746,911 | Δ1,199 bp | Y75_RS18585–[tnaB] | Y75_RS18585, [tnaB] | |
| MC JC | 3,750,591 | Δ1,199 bp | Y75_RS18600 | Y75_RS18600 | |
| MC JC | 3,958,123 | Δ34 bp | coding (1156‑1189/1287 nt) | Y75_RS19595 → | C4‑dicarboxylate transporter DctC |
| RA | 4,136,677 | G→T | V191V (GTC→GTA) | frlD ← | fructoselysine 6‑kinase |
| RA | 4,221,755 | G→A | A353T (GCA→ACA) | aceA → | isocitrate lyase |
| MC JC | 4,310,363 | Δ1,199 bp | Y75_RS21310–[alsK] | Y75_RS21310, [alsK] | |
| RA | 4,371,274 | (A)5→3 | pseudogene (179‑180/1304 nt) | Y75_RS21600 ← | anaerobic C4‑dicarboxylate transporter |
| JC | 4,547,860 | (TCCCTCAGTTCTACAGCGGCTCTG)1→2 | coding (66/549 nt) | fimA → | metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_007779 | 1197822 | 1213071 | 15250 | 85 [80] | [81] 82 | [icd]–[Y75_RS23470] | [icd],Y75_RS05945,Y75_RS05950,lit,Y75_RS05960,Y75_RS05965,Y75_RS24820,Y75_RS23460,Y75_RS05970,Y75_RS05975,Y75_RS05980,ymfL,Y75_RS23465,Y75_RS05995,Y75_RS06000,Y75_RS06005,Y75_RS06010,Y75_RS06015,Y75_RS06020,Y75_RS06025,Y75_RS06030,Y75_RS06035,pinE,Y75_RS06045,[Y75_RS23470] |
| * | * | ÷ | NC_007779 | 3986969 | 3988247–3987186 | 218–1279 | 140 [1] | [80] 83 | [Y75_RS19720]–Y75_RS19725 | [Y75_RS19720],Y75_RS19725 |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_007779 | 273179 = | NA (NA) | 133 (0.930) | 113/496 | 0.4 | 99.2% | intergenic (+2/+146) | Y75_RS01330/Y75_RS01335 | hypothetical protein/IS5‑like element IS5 family transposase |
| ? | NC_007779 | 991031 = | 1 (0.010) | intergenic (‑253/‑13) | pncB/pepN | nicotinate phosphoribosyltransferase/aminopeptidase N | |||||
| * | ? | NC_007779 | = 274373 | NA (NA) | 139 (0.970) | 118/496 | 0.3 | 99.3% | intergenic (‑32/‑176) | Y75_RS01335/mmuP | IS5‑like element IS5 family transposase/S‑methylmethionine permease |
| ? | NC_007779 | = 991034 | 1 (0.010) | intergenic (‑256/‑10) | pncB/pepN | nicotinate phosphoribosyltransferase/aminopeptidase N | |||||
| * | ? | NC_007779 | 380484 = | NA (NA) | 122 (0.850) | 100/496 | 0.9 | NA | coding (28/444 nt) | Y75_RS01845 | transferase |
| ? | NC_007779 | = 381407 | NA (NA) | coding (518/914 nt) | Y75_RS01860 | IS3‑like element IS2 family transposase | |||||
| * | ? | NC_007779 | = 1398952 | NA (NA) | 133 (0.930) | 111/496 | 0.5 | 97.8% | intergenic (+146/‑127) | Y75_RS07025/Y75_RS07030 | IS5‑like element IS5 family transposase/DUF2534 family protein |
| ? | NC_007779 | = 1908959 | 3 (0.020) | coding (191/210 nt) | cspE | transcription antiterminator/RNA stability regulator CspE | |||||
| * | ? | NC_007779 | 1772079 = | 5 (0.040) | 224 (1.570) | 153/496 | 0.0 | 97.8% | intergenic (+179/‑7) | ydiK/rprA | AI‑2E family transporter YdiK/antisense sRNA RprA |
| ? | NC_007779 | 2176983 = | NA (NA) | intergenic (‑3/‑32) | gatA/Y75_RS10980 | PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase | |||||
| * | ? | NC_007779 | = 1772082 | 4 (0.030) | 137 (0.960) | 117/496 | 0.3 | 97.2% | intergenic (+182/‑4) | ydiK/rprA | AI‑2E family transporter YdiK/antisense sRNA RprA |
| ? | NC_007779 | 1980209 = | NA (NA) | intergenic (‑298/+146) | flhD/Y75_RS09980 | flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase | |||||
| * | ? | NC_007779 | 1908956 = | 3 (0.020) | 142 (0.990) | 114/496 | 0.4 | 97.9% | coding (194/210 nt) | cspE | transcription antiterminator/RNA stability regulator CspE |
| ? | NC_007779 | 2176983 = | NA (NA) | intergenic (‑3/‑32) | gatA/Y75_RS10980 | PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase | |||||
| * | ? | NC_007779 | 3128802 = | NA (NA) | 176 (1.230) | 139/496 | 0.0 | 98.3% | coding (34/1137 nt) | Y75_RS15525 | hypothetical protein |
| ? | NC_007779 | 3187655 = | 3 (0.020) | coding (1600/2466 nt) | Y75_RS15850 | fimbrial biogenesis outer membrane usher protein | |||||
| * | ? | NC_007779 | = 3129996 | NA (NA) | 201 (1.410) | 151/496 | 0.0 | 99.0% | intergenic (+146/+1) | Y75_RS15530/Y75_RS15535 | IS5‑like element IS5 family transposase/lipopolysaccharide biosynthesis protein |
| ? | NC_007779 | = 3187658 | 2 (0.010) | coding (1603/2466 nt) | Y75_RS15850 | fimbrial biogenesis outer membrane usher protein | |||||
| * | ? | NC_007779 | = 3186081 | NA (NA) | 153 (1.080) | 120/494 | 0.3 | 99.4% | coding (26/2466 nt) | Y75_RS15850 | fimbrial biogenesis outer membrane usher protein |
| ? | NC_007779 | 3512753 = | 1 (0.010) | coding (529/1026 nt) | cytR | DNA‑binding transcriptional regulator CytR | |||||
| * | ? | NC_007779 | = 3512759 | 1 (0.010) | 171 (1.200) | 134/494 | 0.1 | 99.4% | coding (535/1026 nt) | cytR | DNA‑binding transcriptional regulator CytR |
| ? | NC_007779 | = 3744262 | NA (NA) | intergenic (‑45/+31) | Y75_RS18560/yidZ | IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ | |||||
| * | ? | NC_007779 | = 3744263 | NA (NA) | 137 (0.960) | 115/496 | 0.4 | 97.2% | intergenic (‑46/+30) | Y75_RS18560/yidZ | IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ |
| ? | NC_007779 | 3903274 = | 4 (0.030) | coding (37/825 nt) | Y75_RS19350 | protein bax | |||||
| * | ? | NC_007779 | 3746912 = | NA (NA) | 147 (1.030) | 127/494 | 0.1 | 96.7% | intergenic (‑4/‑31) | Y75_RS18580/Y75_RS18585 | tryptophan permease/IS5‑like element IS5 family transposase |
| ? | NC_007779 | 3840031 = | 5 (0.040) | coding (582/699 nt) | rfaY | lipopolysaccharide core heptose(II) kinase RfaY | |||||
| * | ? | NC_007779 | = 3748105 | NA (NA) | 191 (1.340) | 137/496 | 0.1 | 100% | intergenic (+146/+3) | Y75_RS18585/tnaB | IS5‑like element IS5 family transposase/low affinity tryptophan permease TnaB |
| ? | NC_007779 | = 3840035 | 0 (0.000) | coding (586/699 nt) | rfaY | lipopolysaccharide core heptose(II) kinase RfaY | |||||
| * | ? | NC_007779 | = 3903279 | 3 (0.020) | 148 (1.040) | 124/494 | 0.2 | 98.0% | coding (42/825 nt) | Y75_RS19350 | protein bax |
| ? | NC_007779 | = 4504190 | NA (NA) | intergenic (+10/+167) | Y75_RS22280/Y75_RS22285 | IS3‑like element IS2 family transposase/phosphoethanolamine transferase | |||||
| * | ? | NC_007779 | 3909304 = | 4 (0.030) | 156 (1.090) | 136/496 | 0.1 | 97.5% | intergenic (‑20/‑346) | Y75_RS19370/xylA | D‑xylose ABC transporter substrate‑binding protein/xylose isomerase |
| ? | NC_007779 | = 3988379 | NA (NA) | coding (746/783 nt) | Y75_RS19730 | hypothetical protein | |||||
| * | ? | NC_007779 | = 3909307 | 4 (0.030) | 143 (1.000) | 118/496 | 0.3 | 97.3% | intergenic (‑23/‑343) | Y75_RS19370/xylA | D‑xylose ABC transporter substrate‑binding protein/xylose isomerase |
| ? | NC_007779 | 3987185 = | NA (NA) | pseudogene (3/379 nt) | Y75_RS19720 | hypothetical protein | |||||
| * | ? | NC_007779 | = 3986968 | 1 (0.010) | 139 (0.970) | 119/496 | 0.3 | 99.3% | pseudogene (220/379 nt) | Y75_RS19720 | hypothetical protein |
| ? | NC_007779 | 3987185 = | NA (NA) | pseudogene (3/379 nt) | Y75_RS19720 | hypothetical protein | |||||
| * | ? | NC_007779 | 4310363 = | NA (NA) | 85 (0.600) | 74/496 | 2.8 | 98.8% | intergenic (‑287/‑32) | yjcS/Y75_RS21310 | alkyl sulfatase YjcS/IS5‑like element IS5 family transposase |
| ? | NC_007779 | = 4540919 | 1 (0.010) | intergenic (+208/+375) | Y75_RS22495/Y75_RS22500 | hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase | |||||
| * | ? | NC_007779 | 4310363 = | NA (NA) | 154 (1.080) | 130/496 | 0.1 | 97.5% | intergenic (‑287/‑32) | yjcS/Y75_RS21310 | alkyl sulfatase YjcS/IS5‑like element IS5 family transposase |
| ? | NC_007779 | 4546841 = | 4 (0.030) | coding (125/597 nt) | fimE | type 1 fimbria regulatory protein FimE | |||||
| * | ? | NC_007779 | = 4311556 | NA (NA) | 129 (0.910) | 108/494 | 0.6 | 98.5% | intergenic (+145/+1) | Y75_RS21310/alsK | IS5‑like element IS5 family transposase/allose kinase |
| ? | NC_007779 | 4540915 = | 2 (0.010) | intergenic (+204/+379) | Y75_RS22495/Y75_RS22500 | hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase | |||||
| * | ? | NC_007779 | = 4311557 | NA (NA) | 160 (1.120) | 133/496 | 0.1 | 98.2% | pseudogene (921/921 nt) | alsK | allose kinase |
| ? | NC_007779 | = 4546844 | 3 (0.020) | coding (128/597 nt) | fimE | type 1 fimbria regulatory protein FimE | |||||