breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 547,694 A→G E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,835 +G coding (255/261 nt) Y75_RS02655 → hypothetical protein
RA 556,778 A→C F63V (TTC→GTC)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
MC JC 654,214 Δ1,199 bp Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 899,330 +C coding (391/1128 nt) rlmC → 23S rRNA (uracil(747)‑C(5))‑methyltransferase RlmC
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS porin OmpF/asparagine‑‑tRNA ligase
RA 1,093,686 T→C V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,197,797 C→T H366H (CAC→CAT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,809 C→T T370T (ACC→ACT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,822 T→C L375M (TTA→CTG)  icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,824 A→G L375M (TTA→CTG icd → NADP‑dependent isocitrate dehydrogenase
MC JC 1,301,041 Δ1,336 bp Y75_RS06530Y75_RS06530 Y75_RS06530, Y75_RS06530
RA 1,372,264 A→G E112G (GAG→GGG)  Y75_RS06905 → sugar phosphorylase
RA 1,519,222 G→T V40V (GTG→GTT Y75_RS07640 → orphan toxin OrtT
RA 1,669,601 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,979,957 G→T intergenic (‑46/+398) flhD ← / ← Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← H48 family flagellin FliC
MC JC 2,176,983 Δ1,199 bp Y75_RS10980[gatA] Y75_RS10980, [gatA]
JC 2,179,217 Δ5 bp coding (435‑439/1263 nt) gatZ ← tagatose‑bisphosphate aldolase subunit GatZ
RA 2,245,455 A→G S283P (TCG→CCG)  Y75_RS11290 ← DUF418 family protein
MC JC 2,320,795 Δ1,336 bp Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,866,111 A→G pseudogene (97/993 nt) rpoS ← RNA polymerase sigma factor RpoS
MC JC 3,261,134 Δ1,199 bp Y75_RS16205[tdcD] Y75_RS16205, [tdcD]
RA 3,268,825 A→G T186A (ACA→GCA)  Y75_RS16240 → YhaC family protein
MC JC 3,742,936 Δ1,336 bp Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
MC JC 3,746,911 Δ1,199 bp Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,750,591 Δ1,199 bp Y75_RS18600 Y75_RS18600
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) Y75_RS19595 → C4‑dicarboxylate transporter DctC
RA 4,136,677 G→T V191V (GTC→GTA frlD ← fructoselysine 6‑kinase
RA 4,221,755 G→A A353T (GCA→ACA)  aceA → isocitrate lyase
MC JC 4,310,363 Δ1,199 bp Y75_RS21310[alsK] Y75_RS21310, [alsK]
RA 4,371,274 (A)5→3 pseudogene (179‑180/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter
JC 4,547,860 (TCCCTCAGTTCTACAGCGGCTCTG)1→2 coding (66/549 nt) fimA → metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1197842 1213104 15263 61 [60] [60] 61 [icd]–[Y75_RS23470] [icd],Y75_RS05945,Y75_RS05950,lit,Y75_RS05960,Y75_RS05965,Y75_RS24820,Y75_RS23460,Y75_RS05970,Y75_RS05975,Y75_RS05980,ymfL,Y75_RS23465,Y75_RS05995,Y75_RS06000,Y75_RS06005,Y75_RS06010,Y75_RS06015,Y75_RS06020,Y75_RS06025,Y75_RS06030,Y75_RS06035,pinE,Y75_RS06045,[Y75_RS23470]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 273179 =NA (NA)105 (0.940) 93/496 0.4 99.1% intergenic (+2/+146) Y75_RS01330/Y75_RS01335 hypothetical protein/IS5‑like element IS5 family transposase
?NC_007779 991031 = 1 (0.010)intergenic (‑253/‑13) pncB/pepN nicotinate phosphoribosyltransferase/aminopeptidase N
* ? NC_007779 = 274373NA (NA)96 (0.860) 86/496 0.6 97.0% intergenic (‑32/‑176) Y75_RS01335/mmuP IS5‑like element IS5 family transposase/S‑methylmethionine permease
?NC_007779 = 991034 3 (0.030)intergenic (‑256/‑10) pncB/pepN nicotinate phosphoribosyltransferase/aminopeptidase N
* ? NC_007779 380484 =NA (NA)101 (0.900) 89/496 0.5 NA coding (28/444 nt) Y75_RS01845 transferase
?NC_007779 = 381407 NA (NA)coding (518/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 381401 =NA (NA)77 (0.690) 68/492 1.6 NA coding (512/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381812 NA (NA)pseudogene (174/174 nt) Y75_RS23175 acetyltransferase
* ? NC_007779 = 1398952NA (NA)136 (1.220) 120/496 0.0 98.6% intergenic (+146/‑127) Y75_RS07025/Y75_RS07030 IS5‑like element IS5 family transposase/DUF2534 family protein
?NC_007779 = 1908959 2 (0.020)coding (191/210 nt) cspE transcription antiterminator/RNA stability regulator CspE
* ? NC_007779 1908956 =3 (0.030)125 (1.120) 107/496 0.1 97.7% coding (194/210 nt) cspE transcription antiterminator/RNA stability regulator CspE
?NC_007779 2176983 = NA (NA)intergenic (‑3/‑32) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
* ? NC_007779 = 2293447NA (NA)94 (0.840) 79/496 0.9 100% intergenic (‑32/‑387) Y75_RS11495/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
?NC_007779 2634172 = 0 (0.000)coding (1285/1371 nt) xseA exodeoxyribonuclease VII large subunit
* ? NC_007779 = 26341751 (0.010)99 (0.880) 86/496 0.6 99.0% coding (1288/1371 nt) xseA exodeoxyribonuclease VII large subunit
?NC_007779 = 3129996 NA (NA)intergenic (+146/+1) Y75_RS15530/Y75_RS15535 IS5‑like element IS5 family transposase/lipopolysaccharide biosynthesis protein
* ? NC_007779 = 3186081NA (NA)114 (1.020) 93/494 0.4 98.3% coding (26/2466 nt) Y75_RS15850 fimbrial biogenesis outer membrane usher protein
?NC_007779 3512753 = 2 (0.020)coding (529/1026 nt) cytR DNA‑binding transcriptional regulator CytR
* ? NC_007779 3226437 =2 (0.020)119 (1.060) 105/496 0.1 98.4% intergenic (+114/‑20) Y75_RS16025/ygjJ amino acid permease/protein YgjJ
?NC_007779 3746911 = NA (NA)intergenic (‑3/‑32) Y75_RS18580/Y75_RS18585 tryptophan permease/IS5‑like element IS5 family transposase
* ? NC_007779 = 32264403 (0.030)128 (1.140) 104/496 0.1 97.7% intergenic (+117/‑17) Y75_RS16025/ygjJ amino acid permease/protein YgjJ
?NC_007779 = 3262328 NA (NA)intergenic (+146/+3) Y75_RS16205/tdcD IS5‑like element IS5 family transposase/propionate kinase
* ? NC_007779 = 35127593 (0.030)137 (1.230) 113/494 0.1 97.9% coding (535/1026 nt) cytR DNA‑binding transcriptional regulator CytR
?NC_007779 = 3744262 NA (NA)intergenic (‑45/+31) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
* ? NC_007779 = 3744263NA (NA)112 (1.000) 96/496 0.3 97.4% intergenic (‑46/+30) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
?NC_007779 3903274 = 3 (0.030)coding (37/825 nt) Y75_RS19350 protein bax
* ? NC_007779 3746912 =NA (NA)170 (1.530) 120/494 0.0 99.4% intergenic (‑4/‑31) Y75_RS18580/Y75_RS18585 tryptophan permease/IS5‑like element IS5 family transposase
?NC_007779 3840031 = 1 (0.010)coding (582/699 nt) rfaY lipopolysaccharide core heptose(II) kinase RfaY
* ? NC_007779 = 3748105NA (NA)148 (1.320) 118/496 0.0 99.3% intergenic (+146/+3) Y75_RS18585/tnaB IS5‑like element IS5 family transposase/low affinity tryptophan permease TnaB
?NC_007779 = 3840035 1 (0.010)coding (586/699 nt) rfaY lipopolysaccharide core heptose(II) kinase RfaY
* ? NC_007779 = 39032790 (0.000)142 (1.270) 122/494 0.0 100% coding (42/825 nt) Y75_RS19350 protein bax
?NC_007779 = 4504190 NA (NA)intergenic (+10/+167) Y75_RS22280/Y75_RS22285 IS3‑like element IS2 family transposase/phosphoethanolamine transferase
* ? NC_007779 3909304 =4 (0.040)128 (1.140) 107/496 0.1 97.0% intergenic (‑20/‑346) Y75_RS19370/xylA D‑xylose ABC transporter substrate‑binding protein/xylose isomerase
?NC_007779 = 3988379 NA (NA)coding (746/783 nt) Y75_RS19730 hypothetical protein
* ? NC_007779 = 39093074 (0.040)135 (1.210) 109/496 0.1 97.1% intergenic (‑23/‑343) Y75_RS19370/xylA D‑xylose ABC transporter substrate‑binding protein/xylose isomerase
?NC_007779 3987185 = NA (NA)pseudogene (3/379 nt) Y75_RS19720 hypothetical protein
* ? NC_007779 4310363 =NA (NA)64 (0.570) 57/496 2.7 98.5% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 = 4540919 1 (0.010)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 4310363 =NA (NA)108 (0.970) 90/496 0.5 98.2% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 4546841 = 2 (0.020)coding (125/597 nt) fimE type 1 fimbria regulatory protein FimE
* ? NC_007779 = 4311556NA (NA)99 (0.890) 88/494 0.5 99.0% intergenic (+145/+1) Y75_RS21310/alsK IS5‑like element IS5 family transposase/allose kinase
?NC_007779 4540915 = 1 (0.010)intergenic (+204/+379) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 = 4311557NA (NA)115 (1.030) 96/496 0.3 99.1% pseudogene (921/921 nt) alsK allose kinase
?NC_007779 = 4546844 1 (0.010)coding (128/597 nt) fimE type 1 fimbria regulatory protein FimE